Bang-Poong and related species are an important herbal medicine. However, it is difficult to determine the commercial dry material through anatomical and chemotaxonomical characteristics. Here, we used a PCR-based technique for an accurate discrimination of Bang-Poong and related species. With the RAPD primers, 215 RAPDSs(random amplified polymorphic DNAs) were obtained, and 98% of them showed polymorphic patterns. RAPDs from the four primers were appropriate for the discrimination of S. divaricata
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, those from the six primers for P. japonicum

, those from the four primers for P. terebinthaceum

, and those from the six primers for G. littoralis Fr.

. The specific bands from the primer 425 were obtained and used to develop SCAR (sequence characterized amplified region) markers, based on the sequence information of the RAPD markers. The SCAR primers generated a 215 bp fragment specific to Peucedanum terebinthaceum

, and a 177 bp and a 300 bp fragment specific to G. littoralis Fr.

. As a result, the three SCAR markers were able to discriminate from two Bang-Poong related species.