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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 31, Issue 4 - Dec 2004
Volume 31, Issue 3 - Sep 2004
Volume 31, Issue 2 - Jun 2004
Volume 31, Issue 1 - Mar 2004
Selecting the target year
Interspecific Pollination of Oriental, Martagon and Trumpet Group as Male Parent in Lilium spp.
Lee, Ji-Yong ; Yoon, Eui-Soo ; Lim, Yong-Pyo ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 95~101
DOI : 10.5010/JPB.2004.31.2.095
This study was undertaken to study the effect of interspecific pollination of L. longiflorum and L.
formolongi as the female parent with Oriental, Martagon and Trumpet group as the male parent by cut-style pollination. In the interspecific pollination of L. longiflorum cv. Gelria and Lorina with Oriental group as the male parent, the corresponding fruits obtained immature embryo were 1, 8, and the mean number of embryo per fruit were 11.0, 3.0, respectively. In the interspecific pollination of L.
formolongi cv. Raizan, the corresponding fruits obtained immature embryo were 17, and the mean number of embryo per fruit were 4.0. On the other hand, in the interspecific pollination between L. longiflorum and L.
formolongi as the female parent and Martagon and Trumpet group as the male parent, the pollination of L.
formolongi as the female parent and L. henryi of Trumpet group as the male parent were obtained only 2 fruits, however no embryo.
Sterility of Intersectional OA Lily Hybrids by Somatic Chromosome Doubling and Production of Subsequent Progeny
Chung, Mi-Young ; Chung, Jae-Dong ; Jee, Sun-Ok ; Tuyl J. M. Van ; Lim, Ki-Byung ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 103~108
DOI : 10.5010/JPB.2004.31.2.103
This study was carried out to develop the efficient methods for the subsequent progeny of intersectional hybrid between Oriental and Asiatic lily hybrids. The pollen fertility and germination ability of 3 different allotetraploids (OAOA) after somatic chromosome doubling was ranged from 0 to 80 percent on artificial pollen germination medium. The number of BC
progeny using allotetraploid of F
OA-hybrid as male and female parent was different. The efficiency of BC
progeny production was increased when F
OA-hybrids was used as male rather than as female parent. And in back crosses of F
OA-hybrids with the Asiatic and Oriental hybrids, Asiatic hybrids showed higher efficiency on BC
progeny production. The ploidy level between 2x or 4x Asiatic hybrid and allotetraploid F
OA-hybrid was determined and showed higher progeny production in 2x-4x crosses rather than 4x-4x.
Selection of Transgenic Potato Plants Expressing Both CuZnSOD and APX in Chloroplasts with Enhanced Tolerance to Oxidative Stress
Tang, Li ; Kwon, Suk-Yoon ; Sung, Chang-K ; Kwak, Sang-Soo ; Lee, Haeng-Seoon ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 109~113
DOI : 10.5010/JPB.2004.31.2.109
In order to develop transgenic potato plants with enhanced tolerance to multiple stress, we constructed the transformation vector expressing both superoxide dismutase and ascorbate peroxidase genes in chloroplasts under the control of a stress-inducible SWPA2 promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system. Transgenic potato plants were regenerated on MS medium containing 100mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of foreign genes into the potato genome. When potato leaf discs were subjected to methyl viologen (MV) at 10
M, transgenic plants showed higher tolerance than non-transgenic or vector-transformed plants. To further study we selected the transgenic plant lines with enhanced tolerance against MV. These plants will be used for further analysis of stress-tolerance to multiple environmental stresses.
Micropropagation of Philodendron wend-imbe through Adventitious Multi-bud Cluster Formation
Han, Bong-Hee ; Yae, Byeoung-Woo ; Goo, Dae-Hoe ; Yu, Hee-Ju ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 115~119
DOI : 10.5010/JPB.2004.31.2.115
In order to micropropagate uniform plantlets of Philodendron wend-imbe, the shoot tips were cultured on media supplemented with 0.5-10.0mg/L BA or 0.01-1.0 mg/L thidiazuron (TDZ). The multi-bud clusters from basal part of shoots formed vigorously on media containing 5.0-10.0 mg/L BA or 0.05-0.1 mg/L TDZ. Shoot formation from the bud cluster sections (5-7mm) was achieved favorably on medium with 5.0mg/L BA and 20 g/L sucrose. Lowering of sucrose in medium to 20 g/L was effective for the inhibition of callus growth from basal part of shoots. Growth of shoots and their rooting were favorable on media containing 1.0-2.0 mg/L IBA or 0.1mg/L NAA. The rooted plantlets were acclimatizated effectively in soil mixed with perlite 1: peat moss 1 or peat moss alone.
Mass Propagation of Dicentra spectabilis L. Lemaire Through In vitro Suspension Culture
Lee, Kang-Seop ; Sim, Ock-Kyeong ; Shin, Jeong-Sun ; Choi, Yong-Eui ; Kim, Ee-Yup ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 121~126
DOI : 10.5010/JPB.2004.31.2.121
Bleeding heart (Dicentra spectabilis L. Lemaire) is one of the most valuable wild flower in Korea. This work was conducted for the mass production of somatic embryos through suspension culture and more effective plant regeneration system in Dicentra spectabilis. High-frequency embryogenic callus proliferation was achieved in SH liquid medium supplemented with 1 mg/L 2,4-D. Half-strength SH medium was suitable concentration for somatic embryo induction and germination. About 5,000 embryos were produced per 250
flask after 4 weeks of culture. Germination rate of somatic embryos was decreased when GA
was added in medium. The plantlets showed a 58％ survival rate when transferred to pots after 1 month of culture. The results indicate that micropropagation procedure via somatic embryogenesis can be applied for an efficient mass propagation of Dicentra spectabilis.
High Efficiency Bioreactor Culture System for Mass Proliferation and Bulblet Formation of Allium victorialis var. platyphyllum Makino
Park, So-Young ; Lee, Wi-Young ; Ahn, Jin-Kwon ; Kwon, Young-Jin ; Park, Hae-Chin ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 127~132
DOI : 10.5010/JPB.2004.31.2.127
A suitable bioreactor culture system for shoot proliferation and bulblet formation of Allium victorialis var. platyphyllum Makino was established. Uptake of soluble carbohydrates in different bioreactor culture systems was also analyzed during the entire culture period. Optimal conditions for multiple shoot formation were determined in raft culture (RC) and modified raft culture system (MRC) (13-15 per explant) in which the explants were placed on a net contacting liquid medium. For bulblet formation and enlargement, 93.4％ of shoot clumps formed bulblets at the basal part. Furthermore, they were uniform in size when cultured with ebb & flood system (E&FS). Bulblets harvested from RC and MRC showed vigorous rooting, however, their growth was not uniform. Whereas soluble carbohydrate contents in the bulblets cultured in E&FS were low, starch content was high. Sucrose, glucose and fructose concentrations in the medium of E&FS culture system decreased as bulblet formation and enlargement proceeded, suggesting that external sucrose is taken up to by the cells before it is hydrolyzed.
Effects of Growth Regulators and Culture Environment on ex vitro Rooting and Acclimatization of Apple Rootstock in vitro Propagated
Kwon, Soon-Il ; Kim, Jeong-Hee ; Kang, In-Kyu ; Kim, Mok-Jong ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 133~138
DOI : 10.5010/JPB.2004.31.2.133
Growth of M.9 (Malus domestica Bark. cv) and M.26 (Malus domestica Bark. cv) of dwarf apple rootstock, cultured on MS agar medium in a vessel with ventilating stopper (VS) and then in vivo rooting and acclimatization under combined-treatment by some materials with IBA, were investigated. Concentration of
and ethylene in the vessel with VS was lower then in the vessel with non-VS. Change of temperature and humidity in the vessel with VS was repeated by light condition. Stomatal pares of tissue in the vessel with VS were immediately closed after plantlets were exposed to room humidity but those in the vessel with non-VS were opened after 20 minutes exposure to room humidity. Leaf area and chloroplast index of tissue in the vessel with VS was higher then in the vessel with non-VS. In vivo rooting ratio and acclimatization ratio of M.9 and M.26 was highest in 300mg/L IBA＋3％ sucrose dip-treatment among other combined- treatments.
Effects of Growth Regulators and Sucrose Concentrations on the Bulblet Formation through In Vitro Culture of Scale Segment in Nerine bowdenii
Lee, Seung-Yeob ; Ahn, Jeong-Ho ; Park, Yun-Jum ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 139~143
DOI : 10.5010/JPB.2004.31.2.139
The twin-scale segments of nerine (Nerine bowdenii) were cultured to investigate the influence of NAA, BA and sucrose concentrations on in vitro bulblet formation. The formation of bulblets from twin-scale segments showed a good response both the percentage of bulblet formation and the number of bulblets per explant on MS medium supplemented with 1mg/L NAA and 2 mg/L BA. Formation of bulblet showed the highest efficiency on medium containing 30g/L, and the formation of bulblets was strongly inhibited on medium containing over 90g/L. When the twin-scale segments formed bulblets were subcultured three times to the same medium by 60 day subculture interval, the number of bulblets per explant was 6.5, 7.3 and 8.2 in order of first, second and third. The bulblets over 3mm in diameter were hypertrophied and rooted after transferring to the hormone-free MS medium. The plantlets over 50mm in height were successfully acclimatized in the soil mixed with the same volume of vermiculite and perlite, and the survival rate was over 95％.
Anatomical Characteristics of Hyperhydric Shoots Occuring in In Vitro Culture of Peace Poplar
Kang, Hyo-Jin ; Moon, Heung-Kyu ; Park, So-Young ; Kim, Pan-Gi ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 145~149
DOI : 10.5010/JPB.2004.31.2.145
We investigated the anatomical aspects of vitrification in peace poplar. Comparisons were made with regard to characteristics occurring between hyperhydric and normal shoots in shoot proliferation cultures on MS medium containing 0.2 mg/L BA. Compared with normal plants, hyperhydric plants had thick, curled, and dark green leaves. Hyperhydric stems were thicker and shorter than those of normal stems. When examined under the microscopes, the mesophyll palisade cells of hyperhydric leaves were vacuolated, whereas those of normal leaves contained normal and enriched vacuole with cytoplasm. Generally, the hyperhydric leaves showed poorly developed palisade parenchyma, and revealed irregular and bigger sized intercellular structures in both palisade and spongy parenchyma as well as epidermis cells compare to those of normal leaves. In addition, the hyperhydric leaves had lower stomatal density and bigger sized cell. Vascular tissues of hyperhydic stems were less differentiated because of poorly lignified xylem tissue. The greatly expanded cortical cells and pith appeared to be the main cause of thick stems as compared with normal stems.
Recent Studies of Edible Plant Vaccine for Prophylactic Medicine against Virus-mediated Diseases
Hahn, Bum-Soo ; Park, Jong-Sug ; Kim, Hyeong-Kuk ; Ha, Sun-Hwa ; Cho, Kang-Jin ; Kim, Yong-Hwan ; Kim, Jong-Bum ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 151~161
DOI : 10.5010/JPB.2004.31.2.151
Transgenic plants have been studied as delivery system for edible vaccine against various diseases. Edible plant vaccines have several potential advantages as follows: an inexpensive source of antigen, easy administration, reduced need for medical personnel, economical to mass produce and easy transport, heat-stable vaccine without refrigerator, generation of systemic and mucosal immunity and safe antigen without fetal animal-virus contaminants. The amount of recombinant antigens in transgenic plants ranged from 0.002 to 0.8％ in total soluble protein, depending on promoters for the expression of interested genes and plants to be used for transformation. Throughout the last decade, edible plant vaccine made notable progresses that protect from challenges against virus or bacteria. However edible plant vaccines have still problems that could be solved. First, the strong promoter or inducible promoter or strategy of protein targeting could be solved to improve the low expression of antigens in transgenic plants. Second, the transformation technique of target plant should be developed to be able to eat uncooked. Third, marker-free vector could be constructed to be more safety. In this review we describe advances of edible plant vaccines, focusing on the yields depending on plants/promoters employed and the results of animal/clinical trials, and consider further research for the development of a new plant-derived vaccine.
Effects of Sucrose Concentration on the Production of hGM-CSF in Transgenic Plant Cell Suspension Culture
Lee, Jin-Ok ; Shim, Doo-Hee ; Joo, Chi-Un ; Kim, Dong-Il ; Lee, Dong-Geun ; Lee, Jae-Hwa ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 163~167
DOI : 10.5010/JPB.2004.31.2.163
The effects of sucrose concentration on the secretion of hGM-CSF, total protein and protease into the medium were investigated in transgenic tobacco cells. The dry cell weight (11.22 g/L), hGM-CSF (181.53
/L) and total protein (66.8 mg/L) were detected as highest at 30 g/L sucrose and protease activity (2660 U/L) was highest at 120 g/L sucrose after 5-day culture. However after 10-day culture, the maximum dry cell weight (28.36 g/L) was found at 60 g/L sucrose while the maximum hGM-CSF (95
/L) was appeared at 150 g/L sucrose. The total protein and protease activity was secreted as 52.28mg/L and 3430 U/L, respectively in the same culture.
The Induction Time of Sucrose Active Transport System during the Phloem Cell Development in Suspension Cultures of Streptantus tortus Cotyledon
Cho, Bong-Heuy ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 169~173
DOI : 10.5010/JPB.2004.31.2.169
Parenchyma cells of Streptanthus tortus suspension cultures possessed the different transport system for aldose-formed D-glucose and for ketose-formed D-fructose.
value for D-glucose and D-fructose were 0.28mM and 15.02mM, respectively.
value of D-mannose was 0.44 mM which is similar to the D-glucose transport system, but D-mannose was transported also through its own special uptake system. Parenchyma cells possessed the transport system of L-glucose, but the function of L-glucose was not known at all. Protoplast of parenchyma cells possessed only the monosugars transport system, but didn`t possess the disugars, sucrose transport system. Early developing phloem protoplasts possessed glucose and sucrose transport system at the same time. On the contrary, in the complete developed phloem cells disappeared preexisted glucose transport system in the parenchyma cells, only new induced sucrose transport system existed.ted.
Expression of a Human Histone H1.5 in Transgenic Tobacco Cultured Cells
Kim, Kee-Yeun ; Kwon, Suk-Yoon ; Song, Jae-Young ; Lee, Haeng-Soon ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 31, issue 2, 2004, Pages 175~178
DOI : 10.5010/JPB.2004.31.2.175
Transgenic tobacco (Nicotiana tabacum L. cv Bright Yellow-2) cell lines expressing a human histone H1.5 (referred to as hH1.5), which suppress collagen-induced rheumatoid arthritis, were developed under the oxidative stress-inducible peroxidase (SWPA2) promoter. Tobacco BY-2 cells were transformed by Agrobacterium-mediated method. The kanamycin-resistant calli were selected on the modified MS medium containing 150mg/L kanamycin and 300mg/L claforan. Transgenic cell lines were confirmed by PCR and northern blot analysis. Recombinant hH1.5 (rhH1.5) protein (42 kDa) was also detected by Western blot analysis, showing a different molecular weight of human hH1.5 (32 kDa). These results suggested that a hH1.5 gene was properly introduced in tobacco cultured cells under the control of SWPA2 promoter. The further characterization of rhH1.5 protein remains to be studied.