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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 31, Issue 4 - Dec 2004
Volume 31, Issue 3 - Sep 2004
Volume 31, Issue 2 - Jun 2004
Volume 31, Issue 1 - Mar 2004
Selecting the target year
Development of Doubled-haploid Population and Construction of Genetic Map Using SSR Markers in Rice
Kim, Kyung-Min ; Nam, Wu-Il ; Kwon, Yong-Sham ; Sohn, Jae-Keun ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 179~184
DOI : 10.5010/JPB.2004.31.3.179
A doubled-haploid (DH) population was developed through anther culture of F
plants obtained from a cross between a japonica cultivar, `Nagdongbyeo`, as male parent and a indica cultivar, `Samgangbyeo`, as female parent. Segregation modes for plant length, culm length, panicle length, third internode length, and days to heading in the DH lines showed nearly normal distribution with wide range of variation. A molecular map with 136 simple sequence repeat (SSR) markers was constructed using the DH population. The total map distance was 1,909 cM and the average interval of marker distance was 14 cM.
Development of an Agrobacterium-mediated Transient Expression System for Intact Leaves of Chili Pepper
Seong, Eun-Soo ; Joung, Young-Hee ; Choi, Doil ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 185~190
DOI : 10.5010/JPB.2004.31.3.185
We established a transient gene expression system in chili pepper leaves based on Agrobacterium-mediated transformation of GUS gene. For the best GUS transient expression, two step culture system was adopted. When the Agrobacterium tumefaciens cell density of pre-culture was
0.3, the cells were harvested and diluted to
0.8 with virulence induction medium after cell harvested. The addition of acetosyringone (200
M) in virulence induction step was a key factor for successful transient expression. Additionally, Younger leaves showed more effective transient expression than older leaves. Temporally, the strongest intensity of GUS expression was detected at 2 days after infiltration. These results demonstrate that Agrobacterium-mediated transient expression can be used for a simple in vivo assays of plant promoters, transcription factors and furthermore provide efficient protocol for chili pepper transformation.
Selection of Transgenic Potato Plants Expressing NDP Kinase 2 Gene with Enhanced Tolerance to Oxidative Stress
Li, Tang ; Kwon, Suk-Yoon ; Yun, Dae-Jin ; Kwak, Sang-Soo ; Lee, Haeug-Soon ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 191~195
DOI : 10.5010/JPB.2004.31.3.191
Arabidopsis NDPK2 (AtNDPK2) is a key singaling component that regulate cellular redox state and known to enhance multiple stress tolerance when over-expressed in Arabidopsis plant (Moon et al. 2003). In order to develop transgenic potato plants with enhanced tolerance to multiple stresses, we placed an AtNDPK2 cDNA under the control of a stress-inducible SWPA2 promoter or enhanced CaMV 35S promoter. Transgenic potato plants (cv. Superior and Atlantic) were generated using an Agrobacterium-mediated transformation system and selected on MS medium containing 100 mg/L kanamycin. Genomic Southern blot analysis confirmed the incorporation of AtNDPK2 cDNA into the potato genome. When potato leaf discs were treated with methyl viologen (MV) at 10
M, transgenic plants showed higher tolerance to MV than non-transgenic or vector-transformed plants. The NDPK2 transgenic potato plants will be further used for analysis of stress-tolerance to multiple environmental stresses.
Micropropagation of Lilium longiflorum `Geogia` by Using Bioreactor.
Han, Bong-Hee ; Suh, Eun-Jung ; Yae, Byeoung-Woo ; Yu, Hee-Ju ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 197~201
DOI : 10.5010/JPB.2004.31.3.197
Shoot clusters were induced from bulb scales of Lilium longiflorum `Geogia`, and proliferated on medium containing 0.5 mg/L BA and 0.5 mg/L IAA. Thereafter, these shoot clusters were cultured in 5 L air-lift bioreactors to form and grow normal bulblets. Number of bulblets increased on medium with 30 g/L sucrose, but growth of bulblets was effective on medium with 60 g/L sucrose. The number of bulblets from shoot clusters had no differences, though bulblet growth was very effective on medium with between full and double strength of MS salts. The inoculation of 100 g shoot clusters as a cultural material was suitable for formation and growth of bulblets in 5 L bioreactors. Air-lift type was more effective for the formation and growth of bulblets than that in ebb and flood one, and 200∼300 mL
injection of air was suitable in growth of bulblets.
High Frequency Shoot Formation and Plant Regeneration from Cotyledonary Hypocotyl Explants of Boxthorn (Lycium chinense Mill.) Seedlings
Jo, Man-Hyun ; Ham, In-Ki ; Lee, Bong-Chun ; Kim, Jae-Whune ; Lee, Won-Seok ; Kwon, Suk-Yoon ; Lee, Haeng-Soon ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 203~207
DOI : 10.5010/JPB.2004.31.3.203
To establish high frequency shoot formation from two cultivars (Cheongyangjaerae and Myungan) of boxthorn (Lycium chinense Mill.), hypocotyl segments with cotyledons from seedlings were used as explants. High frequency adventitious shoot formation (more than 80%) were obtained from hypocotyl segments with cotyledon on MS medium supplemented with 1.0 mg/L zeatin, when precultured for 3 weeks under dark conditions followed by transfer to light conditions. But there was no shoot induction in the explants cultured without preculture under dark conditions. Roots were induced from the shoots when transferred to rooting medium supplemented with 1.0 mg/L IAA for 4 weeks. Regenerated plantlets were grown to normal mature plants in soil.
Effects of Membrane Filter and Sucrose Concentrations on the Growth of Balloon Flower (Platycodon grandiflorum A. DC.) Plantlets In Vitro
Choi, So-Ra ; Kim, Myung-Jun ; Eun, Jong-Seon ; Ahn, Min-Sil ; Lim, Hoi-Chun ; Ryu, Jeong ; You, Dong-Hyun ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 209~217
DOI : 10.5010/JPB.2004.31.3.209
The shoots of balloon flower (Platycodon grandiflorum A. DC.) in vitro germinated from seeds were cultured on MS basal medium containing 0.1 mg/L NAA under the various sucrose concentrations and with/without membrane filter (MF) on the lid of vessel. The growth responses were checked to obtain healthy plantlets. The
concentration in vessel without MF were higher than those with MF. The
concentration without MF was increased as days in culture went by whereas the
concentration was decreased. The plant growth with MF and high sucrose concentration was good. Fresh and dry weight of plantlets cultured in sucrose 4.5% with MF were higher than those in no sucrose without MF. Also the content of chlorophyll of plantlets cultured with MF was high and the content of sugar was shown a similar results and a remarkable difference between MF treatments, especially. Stomata cultured with MF was closer than that without MF and mesophyll of leaf were more developed with MF or in high sucrose concentration. When the plantlets were transplanted in the pot at 25
, 75% relative humidity and low PPFD (photosynthetic photon flux density), the percentage of survival after 13 days without MF was 0% but it was 100% with MF regardless of sucrose concentrations.
Mass Propagation of Somatic Embryos and Plantlets of Aralia elata through Bioreactor Culture
Lee, Won-Seok ; Choi, Eun-Gyung ; Kim, Jae-Whune ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 219~223
DOI : 10.5010/JPB.2004.31.3.219
Embryogenic calli were induced from petioles of Aralia elata on MS solid medium supplemented with 1.0 mg/L 2,4-D. When embryogenic calli were transferred to MS liquid medium supplemented with 1.0 mg/L 2,4-D, embryogenic cells and embryogenic cell clusters were developed after 2 weeks of culture. Embryogenic cells were filtered through a 250
sieve and the passed cells were proliferated and maintained in MS liquid medium supplemented with 1.0 mg/L 2,4-D. Embryogenic cell clusters entrapped on the sieve were transferred to 1/2 MS liquid medium without plant growth regulators, globular-shaped embryos were developed from embryogenic cell clusters after 2 weeks of culture. Numerous early stage somatic embryos could be developed to heart-shaped, torpedo-shaped, cotyledonary embryos and plantlets in 5 L bioreactor. Above results suggest that effective somatic embryo proliferation can be achieved via bioreactor culture systems in Aralia elata.
Assessment of Nucleus-DNA Damage in Red Pepper Cells Treated with γ-Radiation through Comet Assay
An, Jung-Hee ; Back, Myung-Hwa ; Kim, Jae-Sung ; Jeong, Jeong-Hag ; Kwon, Soon-Tae ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 225~230
DOI : 10.5010/JPB.2004.31.3.225
We employed single cell gel electrophoresis method (comet assay) to analyze the degree of nucleus-DNA damage in the leaves of red pepper (Capsicum annuum L.) seedlings exposed to
CO v-radiation stress. Nucleus-DNA damage was measured as the ratio of tail length (T) to head length (H) in individual comet image isolated from pepper leaf cell. The T/H ratio of control-cells and treated-cells at 50 or 100 Gy were 1.28 and 3.54 or 3.39, respectively, suggesting that nuclei of pepper cells were severely damaged in the integrity of DNA strand by the treatment of enhanced v-radiation. The percentage of head-DNA in control-cells was 76.8%, whereas those of 50 and 100 Gy treated-cells were 55.9% and 59.9%, respectively. Pretreatment of low dose (4 to 20 Gy) radiation to seeds decreased DNA-damage in the leaves of seedlings treated with high dose radiation at 50 or 100 Gy. In this experiment, we developed a sensitive, reliable and rapid method for evaluating genotoxic effect in the nuclei of plant cells by employing comet assay.
Astaxanthin Biosynthesis in Transgenic Arabidopsis by Using Chyb Gene Encoding β-Carotene Hydroxylase
Lee, Ho-Jae ; Kang, Kwon-Kyoo ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 231~237
DOI : 10.5010/JPB.2004.31.3.231
Oxycarotenoids are oxygenated carotenoids that perform critical roles in plants.
-Carotene hydroxylase adds hydroxyl groups to the
-rings of carotenes and has been cloned from several bacteria and plants including Arabidopsis. This study was carried out to investigate the effect of
-carotene hydroxylase gene (Chyb) on the oxycarotenoids biosynthesis in the transgenic Arabidopsis. Construct of pGCHYB containing Chyb was established onto Gateway vector system (pENTR3C gateway vector and pH2GW7 destination vector). Arabidopsis thaliana (cv. Columbia) was transformed with Agrobacterium tumerfacience GV3101 harboring pGCHYB construct driven by 35S promoter and hygromycin resistant gene. Seven hundred bases paired PCR products, indicating the presence of Chyb gene, were found in the transformants by PCR analysis using Chyb primers. Hygromycin resistance assay showed that transgenes were stably inherited to next generation. The overexpression of the Chyb gene resulted in the decrease carotenoid content. Especially, astaxanthin unusual oxycarotenoid in wild type Arabidopsis was detected in the transgenic plants. This means that decreased carotenoids might be converted into astaxanthin metabolism with the aid of silent gene in the host.
Phytochrome Signal Transduction Regulates Anthocyanin Biosynthesis in Cell Suspension Cultures of Vitis vinifera
Choi, Kwan-Sam ; Kim, Sun-Kyung ; In, Jun-Gyo ; Shin, Dong-Ho ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 239~248
DOI : 10.5010/JPB.2004.31.3.239
This experiment was carried out to confirm that phytochrome regulates anthocyanin bio-synthesis during cell suspension culture system of grape or not. In suspension culture of grape, maximum accumulation of anthocyanin was observed at the stationary phase under continuous white light condition. From mono-chromatic light interruption for 24h at the 4th or 7th day on the suspension cultured cells, the anthocyanin accumulation was highly enhanced at the light interruption at 7th day than 4th day under all monochromatic light treatment. However, the cell growth patterns were not affected by any light treatment. In the darkness, the anthocyanin synthesis was very low but remarkably increased by blue light or red light irradiation. However, the increase of anthocyanin accumulation by blue or red light was suppressed by far-red light in the suspension cells of grape. This suppression by far-red light on the anthocyanin synthesis also observed on the cells treated red or far-red light alternatively. These results implied that phytochrome regulation system may be involved in the anthocyanin biosynthesis of the suspension grape cells. By RNA expression analysis, chalcone synthase (CHS) gene was expressed highly by blue and red light but low by far-red light. The synergistic increase of CHS gene expression was also observed at the treatment of blue light followed by red for 24h. This result may explain the increase of anthocyanin accumulation in B/R treatment. Although the expression of phytochrome gene (PHYA or PHYB) was not highly increased by all light treatment (blue, red, and far-red light) the expression of both PHYA gene and PHYB gene was increased a little in cells treated red or far-red light. In grape suspension cells, the red light enhanced the anthocyanin synthesis, whereas the far-red light was suppressed. Although it was not confirmed whether or not phytochrome gene is activated in anthocyanin accumulating grape cells, we believed that anthocyanin biosynthesis in grape cells may be regulated under phytochrome signal transduction system.
Effects of Various Bioreactors on Growth and Ginsenoside Accumulation in Ginseng Adventitious Root Cultures(Panax ginseng C.A. Meyer)
Kim, Yun-Soo ; Hahn, Eun-Joo ; Paek, Kee-Yoeup ;
Journal of Plant Biotechnology, volume 31, issue 3, 2004, Pages 249~253
DOI : 10.5010/JPB.2004.31.3.249
The type of air lift bioreactor affected the root growth in ginseng adventitious root cultures. Among bioreactors used in this experiment, bulb type bubble bioreactor (BU) was the best to increase root growth (41.92 g dry weight). The kLa value representing the oxygen transfer capacity from medium to explants (6.98 h
) in BU with 5 cm bubble column was higher than other bioreactors. On the other hand, cylindric tube bioreactor (CT) without bubble column resulted in minimum root growth (38.55 g dry weight) and kLa value (5.25 h
). Furthermore, the root growth (50.30 g dry weight) in BU with 10 cm bubble column more increased than 5 cm bubble column. However, the kLa value do not affected the secondary metabolite such as ginsenosides. These results show that the bubble column in air lift bioreactor increase kLa value and increased kLa value stimulate the growth of ginseng adventitious roots.