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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 31, Issue 4 - Dec 2004
Volume 31, Issue 3 - Sep 2004
Volume 31, Issue 2 - Jun 2004
Volume 31, Issue 1 - Mar 2004
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Development of Transgenic Soybean Using Agrobacterium tumefaciens
Cho, Mi-Ae ; Choi, Dong-Woog ; Liu, Jang-Ryol ; Clemente Tom ; Choi, Pil-Son ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 255~259
DOI : 10.5010/JPB.2004.31.4.255
Agrobacterium tumefaciens-mediated cotyledonary node transformation was used to produce transgenic soybean. Cotyledonary node explants of three cultivars and one genotype were co-cultivated with strains Agrobacterium (LBA4404, GV3101, EHA101, C58) containing the binary vectors (pCAMBIA3301 and pPTN289) carrying with CaMV 35S promoter-GUS gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depend on bacteria strain. The EHA101 strain of the bacterial strains employed gave the maximum efficiency (3.6%). One hundred-six lines transformed showed the resistance in glufosinate. Histochemical GUS assay showed that at least 11 plants transformed with the GUS gene were positive response. The soybean transformants were obtained from the Thorne (5 plants), 1049 (5 plants) and Bakun (1 plant), respectively. Southern blot analysis and leaf painting assay revealed that the GUS and bar gene segregated and expressed in their progeny.
Expression in Successive Generations of bar Gene Introduced in Petunia
Ha, Young-Min ; Park, Sang-Mi ; Kim, Zhoo-Hyeon ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 261~266
DOI : 10.5010/JPB.2004.31.4.261
This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacterium-mediated transformation, in successive generation, or after crossing or back-crossing. Some of different 25 transgenic plants were used in crossing and back-crossing to wild type, or repeated-selfing to T
generation. On the processing of experiment, it was found that some lines lost their resistant ability to herbicide basta, or showed non-Mendelian segregation mode: produced much more susceptible segregants than resistant plants. Even though there are exceptional cases, which was off from expected, the genetic stability of bar gene introduced could be confirmed strongly, because in almost case, the segregation of resistant and susceptible plants to basta was done under Mendelian-law according to single gene dominant model.
Agrobacterium- mediated Genetic Transformation and Plant Regeneration of Sweetpotato (Ipomoea batatas)
Lim, Soon ; Yang, Kyoung-Sil ; Kwon, Suk-Yoon ; Paek, Kee-Yoeup ; Kwak, Sang-Soo ; Lee, Haeng-Soon ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 267~271
DOI : 10.5010/JPB.2004.31.4.267
Transformed sweetpotato (Ipomoea batatas (L.) Lam. cv. Yulmi) plants were developed from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105/pCAMBIA2301 harboring genes for intron
-glucuronidase (GUS) and kanamycin resistance. Transient expression of GUS gene was found to be higher when embryogenic calli were co-cultivated with Agrobacterium for 2 days. The co-cultured embryogenic calli transferred to selective MS medium containing 1mg/L 2,4-D, 100mg/L kanamycin, and 400mg/L claforan. These embryogenic calli were subcultured to the same selection medium at 4 weeks interval. Kanamycin-resistant calli transferred to hormone-free MS medium with kanamycin gave rise to somatic embryos and then converted into plantlets in the same medium. Southern blot analysis confirmed that the GUS gene was inserted into the genome of the sweetpotato plants. A histochemical assay revealed that the GUS gene was preferentially expressed in the leaf, petiole, and vascular tissue and tip of root.
Molecular Cloning and Characterization of Outer Envelope Membrane Protein from Salicornia herbacea
Ermawati Netty ; Cha, Joon-Yung ; Liang, Yingshi ; Jung, Min-Hee ; Shin, Dongjin ; Lee, Byung-Hyun ; Lee, Kon-Ho ; Son, Daeyoung ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 273~278
DOI : 10.5010/JPB.2004.31.4.273
Complementary DNA encoding chloroplast outer envelope membrane protein (OEP) from the halophyte Salicornia herbacea has been cloned and sequenced. The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa. The expression level of ShOEP increased by salt, drought and ABA treatments. ShOEP expression was largely induced in roots and shoots by high salts. The biological function of ShOEP was examined by yeast complementation. ShOEP can suppress Na
sensitivity of yeast mutant (cnb
) in the presence of salt. These results suggest that ShOEP is a salt inducible gene and may have functions in the regulation of plant salt stress.ant salt stress.
Impact of Physical, Chemical and Biological Factors on Lily (Lilium longiflorum cv. Georgia) Pollen Growth and GUS Expression Via Agro-infiltration
Park, Hee-Sung ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 279~283
DOI : 10.5010/JPB.2004.31.4.279
To lily (Lilium longflorum cv. Georgia) pollen, impacts by some physical, chemical and biological factors were examined in respects of its growth and transient gene expression via agro-infiltration. Rolling movement in liquid medium or vacuum pressure during Agro-infiltration was regarded as a impact that should be minimized for normal pollen growth. Pollen growth was maintained well in relatively broad range of temperature (19 to 27
) or pH (5.0 to 8.0). Chemical factors such as cefotaxime (up to 300mg/L), acetosyringone (up to 800
M) and syringealdehyde (up to 800
M) did not show any harmful effects but kanamycin severely did even at concentration as low as 25mg/L in some cases. For GUS gene expression, acetosyringone at 200 to 400
M slightly improved the efficiency while syringealdehyde did not. Brief agro-infiltration followed by 18 hr of co-incubation of pollen along with Agrobacterium was suggested as a condition basically required for the transient expression system using lily pollen regardless of the presence of acetosyringone.
Construction of FMDV VP1 Gene Using Artificial DNA Synthesis and Transformation of Nicotiana tabacum Using Agrobacterium Vector System
Lee, Eun-Jung ; Lim, Hee-Young ; Kim, Sung-Hoon ; Kang, Kyung-Sun ; Park, Young-Doo ; Yun, Choong-Hyo ; Yoon, Byoung-Su ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 285~293
DOI : 10.5010/JPB.2004.31.4.285
FMDV is a viral pathogen that caused foot-and-mouth disease in animals. VP1 is a major capsid protein of FMDV. It is known as one of best materials for the FMDV diagnosis and for the development of protein vaccine. In this study, 633 bp of VP1 gene was modified for the expression of VP1 in plant, based on the VP1 DNA sequence from FMDV taiwan O type and from FMDV isolated vietnam. The. deduced DNA fragment was artificially synthesized using the multiple fragment extension with long-nucleotides. A new plant transgenic vector system, pCAMBIA139011 was constructed on the basis of pBI12l and pCAMBIA1390. Using this vector system and GFP gene or modified VP1 gene, each target gene was introduced into Nicotiana tabacum. The insertion of whole target gene was successfully confirmed in each transgenic plant named GFP-A7 and VP1-4, respectively. The expression level of each gene was estimated by RT-PCR and Real-Time PCR using VP1, GFP specific primers.
Induction of Hariy Root and Bioreactor Culture of Lycium chinense
Bae, Ki-Hwa ; Kim, Yun-Soo ; Jeong, Jae-Hun ; Kim, Young-Seon ; Choi, Yong-Eui ; Yoon, Eui-Soo ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 295~300
DOI : 10.5010/JPB.2004.31.4.295
This article was conducted to induce the transgenic hairy roots and determine the effect of culture conditions on optimum growth of hairy roots by Agrobacterium rhizogenes strain, 15834 in Lycium chinense Miller. Hairy roots of L. chinense Miller. were induced from leaf segments by co-cultivation with A. rhizogenes. When the hairy roots were cultured in various MS medium strength and sucrose concentrations, the highest growth of hairy roots was observed in half-strength MS media supplemented with 3% sucrose, respectively. In air lift bioreactor cultures, the liquid medium contained with 1/2 MS and 3% sucrose was also the best for optimum growth of hairy roots.
Morphological Development and Histology of Multiple Shoots and Microbulbs of Garlic Cultured in Bioreactors
Kim, Eun-Kyung ; Hahn, Eun-Joo ; Paek, Kee-Yoeup ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 301~306
DOI : 10.5010/JPB.2004.31.4.301
Multiple shoots of garlic (Allium sativum L.) were propagated in bioreactors containing MS medium supplemented with 2% sucrose for 3 weeks. Microbulbs were induced on MS medium supplemented with 0.1mg/L NAA and 11% sucrose for 9 weeks. For multiple shoot proliferation, leaves in the shoot must be removed before cultures. When the multiple shoots were cultured without removal of leaves, more than 90% of hyperhydricity and no microbulb formation were observed. Histological observation also indicated irregular size and shape of the cells in hyperhydricity of the shoot. Microbulbs were strarted to form from the shoot after 7 weeks of culture by protuberance of adventitious shoot buds followed by inner periclinal divisions and simultaneous anticlinical division in the epidermis of meristematic bulge. Analysis of ploidy level indicated no phenotypic variations in both multiple shoots and microbulbs induced from the mother plant, suggesting genetic homogeneity among the regenerants.
Development of an Automated Control System for Bioreactor using the Plant Tissue Culture
Chung, Seok-Hyun ; No, Daehyun ; Kang, Changho ; Kang, Sukwon ; Han, Bong-Hee ; Lee, Gee-Myung ; Na, Young-Sun ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 307~312
DOI : 10.5010/JPB.2004.31.4.307
The bioreactor system for the large-scale plant tissue culture was developed to control the pH concentration and DO (dissolved oxygen), and air flowrate. The system controlling the proper air flow rate for each bulblet growth stage and monitoring the contamination of bioreactor using the pH change was controled by computer program. For the uniform bulblet distribution in bioreactor, the proper air flow rate was 300 cc/min at the beginning of bulblet culture, 400 cc/min after 20 days, 500 cc/min after 40 days, 600 cc/min after 60days, and 700 cc/min after 80 days. It was possible to maintain the pH concentration within 5.5
0.5 during the culture by control system of bioreactor.
Stress-induced Activity of Matrix Metalloproteinase in Tobacco Plants
Oh, In-Suk ; So, Sang-Sup ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 313~317
DOI : 10.5010/JPB.2004.31.4.313
Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases produced by a variety of cell type, and have a fundamental role in the degradation and remodeling of extracellular matrix. In this study, we screened the secretion of MMPs in leaves of different developmental stages and in response to environmental stress using tobacco. Compare with fully maturing leaves and older leaves, the rate of MMPs activity was high in expanding and younger leaves. It is tempting to speculate that MMPs may be involved in tissue modeling, which must occur during leaf expansion. The MMPs activity in tobacco leaves grown in the presence of stressors showed a significantly increase at salinity treatment and pathogen infection. The MMPs activity in salinity and pathogen treatment increased respectively, by 1.2- and 1.5-fold with respect to the control. These results suggest that MMPs may be involved in plant defence against adverse environment and pathogenic infection.
Characterization of 65 kD Protein in Latex Excreted from Euphorbia lathyris
Park, Hee-Sung ;
Journal of Plant Biotechnology, volume 31, issue 4, 2004, Pages 319~323
DOI : 10.5010/JPB.2004.31.4.319
Soluble latex protein fraction excreted from Euporbia lathyris laticifer was resolved by 10% SDS-polyacrylamide gel electrophoresis to identify distinctively displayed latex major protein bands including ELp65, ELp55, ELp43, ELp32 and ELp23. Among them, ELp65 was purified by ammonium sulfate precipitation, gel permeation chromatography and ion exchange chromatography. Its N-terminal amino acid sequencing revealed its homology to the leading region of mature peptide of tomato p69a subtilisin-like protease, suggesting a certain role involved in plant defense system. In the analysis of Southern blot hybridization using PCR-amplified tomato p69a probe DNA, E. lathyris genome was suggested to have a gene family consisting of 3-5 gene members putatively encoding subtilisin-like proteases.