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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 32, Issue 4 - Dec 2005
Volume 32, Issue 3 - Sep 2005
Volume 32, Issue 2 - Jun 2005
Volume 32, Issue 1 - Mar 2005
Selecting the target year
Molecular Mechanism of Plant Immune Response
Kwon Tack-Min ; Nam Jae-Sung ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 73~83
DOI : 10.5010/JPB.2005.32.2.073
Disease resistance in plants is often controlled by gene-for-gene mechanism in which avirulence (avr) gene products encoding by pathogens are specifically recognized, either directly or indirectly by plant disease resistance (R) gene products and sequential signal transduction pathways activating defense responses are rapidly triggered. As a results, not only exhibit a resistance against invading pathogens but also plants maintain the systemic acquired resistance (SAR) to various other pathogens. This molecular interaction between pathogen and plant is commonly compared to innate immune system of animal. Recent studies arising from molecular characterization of a number of R genes from various plant species that confer resistance to different pathogens and corresponding avr genes from various pathogens resulted in the accumulation of a wealth of knowledge on molecular mechanism of gene-for-gene interaction. Furthermore, new technologies of genomics and proteomics make it possible to monitor the genome-wide gene regulation and protein modification during activation of disease resistance, expanding our ability to understand the plant immune response and develop new crops resistant to biotic stress.
Agronomic Characteristics of Transgenic Japonica Rice 'Milyang 204' with Herbicide Resistance Gene (bar)
Jeong Eung-Gi ; Yi Gi-Hwan ; Won Yong-Jea ; Park Hyang-Mi ; Cheon Nam-Soo ; Choi Jun-Ho ; Ku Yeon-Chung ; Han Chang-Deok ; Eun Mu-Yeong ; Kim Tae-Sas ; Nam Min-Hee ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 85~90
DOI : 10.5010/JPB.2005.32.2.085
This study was conducted to investigated the major characteristics of genetically modified rice of 'Milyang 204' originated from Dongjinbyeo compared to a non-transgenic rice varieties Dongjinbyeo and Jun-ambyeo. Basta resistant transgenic rice lines carrying bar gene produced by the Yeongnam Agricultural Research Institute were evaluated for their agronomic characters. The transgenic Japonica rice of 'Milyang 204' showed inferior phenotypic traits compared to a non-transgenic rice variety Dongjinbyeo and Junambyeo. On the basis of UPOV (Union Internationale Pour la Protaection des Obtentions Vegetables) and NSMO(National Seed Management Office) the transgenic 'Milyang 204' showed difference in some traits out of some agronomic traits, such as leaf color, angle of flag leaf, number of spikelets, culm length, white core and white belly compared to the non-transgenic varieties rice.
Production of Transgenic Maize (Zea mays L.) Using Agrobacterium tumefaciens-Mediated Transformation
Cho Mi-Ae ; Park Yun-Ok ; Kim Jin-Suck ; Park Ki-Jin ; Min Hwang-Ki ; Liu Jang-Ryol ; Clemente Tom ; Choi Pil-Son ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 91~95
DOI : 10.5010/JPB.2005.32.2.091
Agrobacterium tumefaciens-mediated immature embryo transformation was used to produce transgenic maize. Immature embryo of Hi II genotype were co-cultivated with strains Agrobacterium tumefaciens (C58C1) containing the binary vectors (pPTN290) carrying with Ubiquitin promoter-GUS gene as reporter gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent. Seven embryogenic callus lines transformed showed the resistance in paromomycin antibiotics. Histochemical GUS assay showed that 7 individual lines transformed with the GUS gene were positive response among the transformants. Southern blot analysis revealed that the nptll gene segregated and expressed in their progeny.
Antioxidative Responses of Transgenic Tobacco Plants Expressing both Superoxide Dismutase and Ascorbate Peroxidase in Chloroplasts to Several Herbicides
Kim Jin-Seog ; Lee Byung-Hoi ; Kwon Suk-Yoon ; Kim Yun-Hee ; Kim So-Hee ; Cho Kwang-Yun ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 97~103
DOI : 10.5010/JPB.2005.32.2.097
Antioxidative responses of transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts was investigated with several herbicides. In greenhouse test, tolerance of SOD/APX-overexpressed tobacco (CA) to photosystem (PS) I inhibitor paraquat was increased by about 40%. However, any response differences between CA and wild type (WT) tobacco was not observed in a treatment with PS II inhibitors (bromoxynil, diuron and bromacil), chlorophyll biosynthesis inhibitor(oxyfluorfen), carotenoid biosynthesis inhibitor (fluridone) and 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibitor (glyphosate). This tendency was also similar in the growth chamber test of low light intensity, using paraquat and diuron. That is, increased antioxidant activity of CA was shown only in paraquat treatment. When paraquat was foliar-treated to 6 to 9-leaf stage plant, the third to fourth placed leaf from shoot tip showed relatively higher antioxidant activity. Ascorbate supplemented to paraquat solution alleviated the phytotoxicity with a similar range in both CA and WT. In conclusion, CA specifically responded to oxidative stress induced by paraquat among tested herbicides in a whole plant assay.
Optimization of Treatment Concentration and Screening of Exogenous Plant Growth Regulators for Improvement of Salidroside Yield in Rhodiola sachalinensis A. Bor Cell Suspension Cultures
Choi Hye-Jin ; Kim Su-Jeong ; Hwang Baik ; Ahn Jun-Cheul ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 105~109
DOI : 10.5010/JPB.2005.32.2.105
To enhance salidroside productivity from a cell suspension cultures of Rhodiola sachalinensis, various combinations of auxin (NAA) and cytokinins (BA, Kinetin) concentration and addition of
, TDZ, zeatin, spermine and spermidine at a hormone combination to be established were examined. NAA/BA combination is superior to NAA/kinetin combination in biomass and salidroside content. Maximum salidroside production (
medium) was obtained from
medium with 1 mg/L NAA and 5 mg/L BA. The adding of
) was beneficial for salidroside accumulation and the highest productivity of salidroside,
, was obtained from
medium supplemented with 1 mg/L NAA, 5 mg/L BA and 0.1 mg/L
. On TDZ, zeatin, spermine and spermidine, expectant results were not obtained except a little affirmative effect of spermidine (
at 1 mg/L).
Effects of Aeration Rate and Sparger Type on Growth and Ginsenoside Accumulation in Bioreactor Cultures of Ginseng Adventitious Root(Panax ginseng C.A. Meyer)
Kim Yun-Soo ; Hahn Eun-Joo ; Shin Cha-Gyun ; Paek Kee-Yoeup ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 111~116
DOI : 10.5010/JPB.2005.32.2.111
The two different ways to supply air inside the bioreactor were examined in the adventitious root cultures of Panax ginseng C.A. Meyer. First, the aeration rate varied at 0.05, 0.1, 0.2 and 0.3 vvm, respectively which were supplied during the whole culture period. Second, the amount of air supply was increased from 0.05 to 0.3 vvm at 10-day intervals in proportion to the root growth. Both the root growth and the ginsenoside accumulation were maximized to 175.8 g dry wt. of root growth and 4.3 mg/g dry wt. of ginsenoside accumulation when the aeration rate was increased gradually. The effect of the sparger pore size (15, 30 and
) in the bioreactor was also investigated, which suggested the greatest root growth (175.9 g dry wt.) in the
-sized sparger and the highest ginsenoside content (4.3 mg/g dry wt.) in the
size. Finally, the diameter of a sparger (
-sized) varied at 1.5, 3.0, 5.0 and 8.0 cm, respectively. The highest root growth (191.9 g dry wt.) and the ginsenoside content (4.9 mg/g dry wt.) were obtained in the sparger diameter of 8.0 cm.
Yellowish Friable Embryogenic Callus (YFEC) Production and Plant Regeneration from Immature Embryo Cultures of Domestic Maize Cultivars and Genotypes (Zea may L.)
Cho Mi-Ae ; Park Yun-Ok ; Kim Jin-Suck ; Park Ki-Jin ; Min Hwang-Ki ; Liu Jang-Ryol ; Choi Pil-Son ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 117~121
DOI : 10.5010/JPB.2005.32.2.117
Immature embryos of 3 cultivars (Du Me Chal, Mi Baek Chal, Heug Jeom Chal) and 5 genotypes (HW1, KL103, HW3, HW4, KW7) were cultured on medium containing MS salts, Eriksson's vitamins, 1 mg/L 2,4-dichlorophenoxyacetic acid, 25 mM proline, 100 mg/L casamino acid, 3 mM MES, 1.7 mg/L
and 20 g/L sucrose (SIM). Frequency of somatic embryo formation on explant of immature embryos showed in HW1 (45.20%), KL103 (5.75%), HW3 (37.20%), HW4 (30.10%), KW70 (55.20%), Mi Baek Chal (18.74%), Heug Jeom Chal (22.41%), Du Me Chal (36.72%) and Hi II type (<10%), respectively. Yellowish friable embryogenic callus (YFEC) such as type II callus of Hi II genotype only produced from the HW3 and Heug Jeom Chal, whereas other cultivars and genotypes were directly formed somatic embryos with late-embryonic stages or expanded yellowish compact somatic embryo with morphological abnormality. The yellowish friable embryogenic callus (YFEC) could be proliferated on the same medium, which were maintained embryogenic capacity for 6 months over. Upon transfer to first regeneration and second regeneration medium, somatic embryos converted to plantlets at a frequency of approximately 100%. However, the expanded somatic embryos with abnormal morphology were slowly proliferated when subcultured on the same medium, and some of them were degenerated or converted to plantlets at a frequency of approximately 25%. Accordingly, The Heug Jeom Chal and HW3 genotype will be further used for development of high frequency transformation system in domestic maize germplasm.
Bulblet Differentiation through the Formation of Friable Embryogenic Callus from Bulb Scales of Lilium longiflorum 'Nellie White'
Han Bong-Hee ; Lee Soo-Young ; Shu Eun-Jung ; Woo Jong-Gyu ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 123~128
DOI : 10.5010/JPB.2005.32.2.123
A series of experiments were performed to establish regeneration system through friable embryogenic callus (FFC) of Lilium longiflorum 'Nellie White'. Only hard and regular callus was induced from bulb scales on medium containing 2.0 mg/L dicamba and
g/L sucrose. The induced hard callus was subcultured on medium with 2.0 mg/L dicamba and 30 g/L sucrose, and used as a material for induction of FEC. In order to induce FEC, induced hard and regular callus was chopped into
segments, and re-cultured on medium with 2.0 mg/L dicamba and 90 g/L sucrose. FEC was induced from chopped hard calli by the subcultures of two months interval. The induction rate of FEC was enhanced when hard callus was subcultured on same medium. FEC was proliferated more than 5 times on medium with
dicamba and 90 g/L sucrose. Bulblet differentiation from FEC was very favorable on MS medium supplemented with 0.1 mg/L BA, 1.0 mg/L NAA and 30 g/L maltose, but many differentiated bulblets were changed to vitrificated ones. The differentiation of normal bulblets was most effective on medium containing
activated charcoal and 30 g/L sucrose.
Effect of Growth Regulators and Osmoticums on Somatic Embryogenesis and Plants Regeneration in Aralia elata Cultivar 'Zaoh'
Kim Ji-Ah ; Moon Heung-Kyu ; Kim Yong-Wook ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 129~134
DOI : 10.5010/JPB.2005.32.2.129
Effective micropropagation system via somatic embryognesis was established for a Phytophthora resistant Aralia elata cultivar. Different kinds of growth regulators were needed to induce embryogenic callus with different explant sources. When leaf explants were used, a combination of 2,4-D, TDZ and L-glutamine was needed, whereas when petiole and root explants needed only 1.0 mg/L 2,4-D. Embryogenic callus induction rate under the optimum culture condition was 75.0%, 67.0% and 83.0% from leaf, petiole and root segment, respectively. Somatic embryo germination and plantlet conversion rate appeared to be influenced greatly by various osmoticums. More than 90% of embryos germinated when treated with sucrose, glucose and maltose. However, the highest conversion rate (72%) was recorded on medium with 2% sucrose only. The converted plantlets grew normally on 1/2MS basal medium, were acclimatized on artificial soil mixture and survived more than 95% in the greenhouse condition. The results suggest that the species can be clonally propagated through in vitro culture system via somatic embryogenesis.
Relationship of Cotyledon Number with Procambium Differentiation in Somatic Embryogenesis of Codonopsis lanceolata L.
Choi Pil-Son ; Soh Woong-Young ; Cho Duck-Yee ; Liu Jang-Ryol ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 135~138
DOI : 10.5010/JPB.2005.32.2.135
Embryogenic callus was obtained from cotyledonary explants of Codonopsis lanceloata on Murashige & Skoog's medium supplemented with 1 mg/L 2,4-D. Suspension cultures of the embryogenic calli were grown on a shaker at 100 strokes/min, and then the calli were subcultured for 2 weeks in 2,4-D-free medium to produce somatic embryo. In somatic embryos at the globular stage, cotyledon initials began to differentiate themselves in the near distal end of the procambial strand. Dicotyledons, tricotyledon, tetracotyledon and fused cotyledon were differentiated from the distal ends of two, three, four and circular procambial strands, respectively. Nearly circular procambial strand in lower hypocotyls were independently differentiated into two, three, four procambial tissues at cotyledonary node and cotyledons to form somatic embryos with dicotyledon, tricotyledon, tetracotyledon. If the distal subepidermal cells of globular embryo exclusively became cotyledon initials, the torpedo or cotyledonary embryo was characterized by somatic embryos with fused cotyledon.
Effect of Growth Retardants on Endogenous ABA-like Substance Content and GA-like Substance Activity of Sedirea japonica Seedlings Cultured In Vitro
Cho Dong-Hoon ; Jee Sun-Ok ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 139~144
DOI : 10.5010/JPB.2005.32.2.139
This experiment was conducted to identify the effect of several plant growth retardants on endogenous ABA-like substance content and GA-like substance activity in seedlings of Sedirea japonica cultured in vitro. When seedlings of Sedirea japonica were treated with low concentration of 0.05 mg/L Uniconazole, 0.1 mg/L Ancymidol and 0.3 mg/L Paclobutrazol, the content of ABA-like substances of the leaf was lower than that of the control. However, the activity of GA-like substances was similar or higher in treated seedlings. In the mid and high concentrations of three kinds of growth retardants, the ABA-like substance content was increased, but GA-like substance activity was inhibited. The content of ABA-like substances in the root was lower in 0.05 and 0.2 mg/L Uniconazole, 0.2 mg/L Ancymidol and 0.1 mg/L Paclobutrazol treatments than that of the control, but in the mid and high concentration treatments, the content was increased. GA-like substance activity in low concentration was increased but in the mid and high concentration, the activity was inhibited compared with low concentration treatment.
Effect of Growth Retardants on Free Sugar and Protein Content of Sedirea japonica Seedlings Cultured In Vitro
Cho Dong-Hoon ; Jee Sun-Ok ;
Journal of Plant Biotechnology, volume 32, issue 2, 2005, Pages 145~149
DOI : 10.5010/JPB.2005.32.2.145
This experiment was conducted to identify the effect of several plant growth retardants on changes of endogenous free sugar and protein content in seedlings of Sedirea japonica cultured in vitro. The content of free sugar in the leaf was decreased as the treated growth retardant concentration was increased. Glucose content was higher than fructose and sucrose content in the leaf. Free sugar content of the root was increased as concentrations of growth retardants were increased. Sucrose content was higher compared with the content of fructose and glucose. The content of protein in the leaf was decreased as the growth retardants concentration was increased, but the tendency of protein content in the root was contrary to that in the leaf.