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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
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Volume & Issues
Volume 32, Issue 4 - Dec 2005
Volume 32, Issue 3 - Sep 2005
Volume 32, Issue 2 - Jun 2005
Volume 32, Issue 1 - Mar 2005
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Transgenic Plants with Enhanced Tolerance to Environmental Stress by Metabolic Engineering of Antioxidative Mechanism in Chloroplasts
Kwon Suk-Yoon ; Lee Young-Pyo ; Lim Soon ; Lee Haeng-Soon ; Kwak Sang-Soo ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 151~159
DOI : 10.5010/JPB.2005.32.3.151
Injury caused by reactive oxygen species (ROS), known as oxidative stress, is one of the major damaging factors in plants exposed to environmental stress. Chloroplasts are specially sensitive to damage by ROS because electrons that escape from the photosynthetic electron transfer system are able to react with relatively high concentration of
in chloroplasts. To cope with oxidative stress, plants have evolved an efficient ROS-scavenging enzymes such as superoxide dismutase (SOD) and ascorbate peroxidase (APX), and low molecular weight antioxidants including ascorbate, glutathione and phenolic compounds. To maintain the productivity of plants under the stress condition, it is possible to fortify the antioxidative mechanisms in the chloroplasts by manipulating the antioxidation genes. A powerful gene expression system with an appropriate promoter is key requisite for excellent stress-tolerant plants. We developed a strong oxidative stress-inducible peroxidase (SWPA2) promoter from cultured cells of sweetpotato (Ipomoea batatas) as an industrial platform technology to develop transgenic plants with enhanced tolerance to environmental stress. Recently, in order to develop transgenic sweetpotato (tv. Yulmi) and potato (Solanum tuberosum L. cv. Atlantic and Superior) plants with enhanced tolerance to multiple stress, the genes of both CuZnSOD and APX were expressed in chloroplasts under the control of an SWPA2 promoter (referred to SSA plants). As expected, SSA sweetpotato and potato plants showed enhanced tolerance to methyl viologen-mediated oxidative stress. In addition, SSA plants showed enhanced tolerance to multiple stresses such as temperature stress, drought and sulphur dioxide. Our results strongly suggested that the rational manipulation of antioxidative mechanism in chloroplasts will be applicable to the development of all plant species with enhanced tolerance to multiple environmental stresses to contribute in solving the global food and environmental problems in the 21st century.
The Use of Glufosinate as a Selective Marker for the Transformation of Cucumber (Cucumis sativus L.)
Cho Mi-Ae ; Song Yun-Mi ; Park Yun-Ok ; Ko Suck-Min ; Min Sung-Ran ; Liu Jang-Ryol ; Choi Pil-Son ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 161~165
DOI : 10.5010/JPB.2005.32.3.161
Agrobacterium tumefaciens-mediated cotyledonary explants transformation was used to produce transgenic cucumber. Cotyledonary explants of cucumber (c.v., Eunchim) were co-cultivated with strains Agrobaderium (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 355 promoter-gus gene as reporter and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selectable marker. There was a significant difference in the transformation frequency depending Agrobacterium strains. The EHA101 of bacterial strains employed gave the maximum frequency (0.35%) for cucumber transformation. Histochemical gus and leaf painting assay showed that 15 individual lines were transgenic with the gus and bar gene. Southern blot analysis also revealed that the gus gene was successfully integrated into each genome of transgenic cucumber.
Molecular Characterization of Cinnamate 4-Hydroxylase gene in Red Hot Pepper (Capsicum annuum L.)
Kim Kye-Won ; Ha Sun-Hwa ; Cho Kang-Jin ; Kim Eun-Ju ; Lee Min-Kyung ; Yu Jae-Ju ; Kim Jong-Guk ; Lee Shin-Woo ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 167~173
DOI : 10.5010/JPB.2005.32.3.167
Three different cDNAS for cinnamate 4-hydroxylase (C4H) which are involved in the second step of the general phenylpropanoid pathway were isolated and designated as pc4h1 (1,755 bp), pc4h2 (1,655 bp), and pc4h3 (1,316 bp), respectively. The nucleotide sequence analysis revealed that both pc4h1 and pc4h2 clones encode polypeptides of 505 amino acids frame but pc4h3 clone was truncated at the 5'-end of coding region. The alignment of the deduced amino acid sequences showed that PC4H1 and PC4H2 are highly homologous (95.8% identical) with each other and contain three conserved domains which are typical in cytochrome P450 monooxygenase: proline-rich region, threonine-containing binding pocket for the oxygen molecule, and heme binding region. In addition, result of the phylogenic tree analysis revealed that both pepper C4Hs belong to Class 1. pc4h2 transcription was strongly induced in wounded fruit (400%) and root (200%) relative to its very low basal level but not in leaf or stem tissue. In case of pc4h1, the basal level of transcription was higher than pc4h2 but induction by wounding was lower in fruit and root while leaf and stem tissues did not respond to wounding. The basal level of pc4h3 transcripts was not, if any, detectable and response to wounding was not observed.
High Frequency Plant Regeneration from Leaf Explant Cultures of Domestic Cultivated Strawberry (Fragaria x ananassa Duch)
Cho Mi-Ae ; Choi Kyu-Myeong ; Ko Suck-Min ; Min Sung-Ran ; Chung Hwa-Ji ; Liu Jang-Ryol ; Choi Pil-Son ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 175~179
DOI : 10.5010/JPB.2005.32.3.175
To develop a high efficiency plant regeneration system from in vitro cultures of strawberry, cv. Yeobong, petiole and leaf explants were cultured on MS basal medium containing a combination of 0.5 mg/L IBA and 3.2 mg/L kinetin or zeatin or benzyl amino purine (BAP) for 6 weeks, and leaf explants with dark pretreatment for a week (
), 2 weeks (
), and 4 weeks (
) were cultured on medium supplemented with 0.5 mg/L IBA and 3.2 mg/L zeatin under 16 hr photoperiod for 6 weeks. Shoot organogenesis was observed from the greenish calli containing minimal anthocyanin formed at proximal cutting edges of the leaf explant (57%) when cultured adaxial side on the medium, whereas was directly formed from a cutting edges of petiole explant (6.3%). Frequency of callus formation and shoot organogenesis at large size of leaf explant (
) was higher than small size (
), and dark pretreatment significantly improved the frequency of leaf explants that produced calli and shoots. The maximum frequency (87%) for shoot organogenesis was obtained from the leaf explants that transferred to a 16 hr photoperiod condition after the initial 4 weeks dark period. The improved frequency (87%) in comparision with control without dark pretreatment (27%). When the shoots were transferred to 1/2 MS basal medium, formed roots with 20 d of culture. The rooted plants were subsequently transferred to the pots and to the field.
Changes of Root Physiology of Tissue Cultured M.9 Apple Rootstock after Layering
Kwon Soon-Il ; Kim Mok-Jong ; Kang In-Kyu ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 181~186
DOI : 10.5010/JPB.2005.32.3.181
This work was conducted to evaluate the effects of rooting on tissue cultured M.9 (Malus domestica Bork. cv, tcM.9) after layering in field. We investigated an appearance period of first root in shoot, rooting ratio, contents of indole-3-acetic acid (IAA), abscisic acid (ABA), inorganic matters, sugars, and lignin in rooting areas of stems by layering. First root in shoot of tcM.9 and natural M.9 appeared 25 and 30 days after layering (DAL), respectively. Rooting ratio was much higher in tcM.9 than in natural M.9. The content of IAA was higher in tcM.9 than in natural M.9 before layering, but it was reversed at 20 and 30 DAL. In contrast, the content of ABA was much higher in natural M.9 than in tcM.9 in case of both before and 10 and 20 DAL. The contents of N, B, Mn, and Zn were significantly higher in tcM.9 than in natural M.9 both before and 10 and 20 DAL. The contents of sugars in tcM.9 had the similar pattern of the contents of inorganic materials. There were statistically significant differences in the contents of sucrose and glucose at 30 DAL as well as the content of maltose at 20 and 30 DAL. The content of lignin was significantly higher in tcM.9 than in natural M.9 before layering and 10 and 30 DAL while there was no difference 20 DAL. Therefore, improvement of rooting ability in the tissue cultured root stock M.9 might be due to the changes of inorganic matters or lignin rather than that of sugars and hormones.
Efficient Callus Culture and Plant Regeneration from Mature Seed of Tall Fescue (Festuca arundinacea Schreb.)
Kim Do-Hyun ; Lee Dong-Gi ; Lee Sang-Hoon ; Woo Hyun-Sook ; Lee Ki-Won ; Choi Myung-Suk ; Lee Byung-Hyun ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 187~193
DOI : 10.5010/JPB.2005.32.3.187
In an effort to optimize tissue culture conditions for genetic transformation of tall fescue (Festuca arundinacea Schreb.), an efficient plant regeneration system from seed-derived calli was established. MS medium containing 6 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.1 mg/L benzyladenine (BA) were optimal for embryogenic callus formation from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency above 50% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 1 mg/L 2,4-D and 3 mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. 'Kentucky-31' showed to have high frequencies of embryogenic callus induction and plant regeneration up to 58.3 and 50%, respectively. Addition of sucrose to the regeneration medium as a carbon source increased regeneration frequency up to 55%. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of tall fescue through genetic transformation.
Effect of Cytokinin and Putrescine on Plant Regeneration from Leaf Explant of Rhodiola sachalinesis A. Bor
Bae Ki-Hwa ; Lim Soon ; Yoon Eui-Soo ; Shin Cha-Gyun ; Kim Yoon-Young ; Kim Yun-Soo ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 195~199
DOI : 10.5010/JPB.2005.32.3.195
The effects of cytokinin and putrescine on adventitious shoot induction from leaf explant of Rhodiola sachalinensis A. Bor were investigated. Among cytokinin used in this experiment, BA was more effective on adventitious shoot induction and shoot elongation than kinetin. Especially, 1 mg/L BA was the best to increase adventitious shoot induction (71%) and shoot elongation (3.0 mm). In addition, 100 mM putrescine in MS medium with 1 mg/L BA was higher in adventitious shoot induction (93%) and shoot elongation (3.8 mm) than single treatment of 1 mg/L BA. Adventitious shoots induced in this experiment rooted on 1/2 MS medium and acclimated over 95% on composed soil (peatmoss:sand=1:1).
Effect of BA and NAA on Adventitious Bud Induction From In Vitro Germinant Eucalyptus pellita
Kim Ji-Ah ; Moon Heung-Kyu ; Kang Ho-Duck ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 201~207
DOI : 10.5010/JPB.2005.32.3.201
This study was conducted to examine the effect of BA and NAA on adventitious bud induction from in vitro germinants E. pellita. The capacity of adventitious bud formation greatly depends on juvenility and explants origin; the more juvenile materials are the better ability to form adventitious buds even in in vitro raised plantlets. In case of in vitro germinants, 7 day old plantlets showed a better morphological response than did 14 day old ones in the induction of adventitious buds. The capacity to show morphological response was in decreasing order : cotyledons> petioles> roots. Ho adventitious buds formed when root segments were used as culture material. And optimum medium appeared to be MS + 0.5 mg/L BA and 0.2 mg/L NAA. Adventitious buds could be developed into multiple shoots and regenerated normal plantlets on DKW medium plus 0.2 mg/L BA and 0.01 mg/L NAA.
Transgenic Siberian Ginseng Cultured Cells That Produce High Levels of Human Lactoferrin
Jo Seung-Hyun ; Kwon Suk-Yoon ; Kim Jae-Whune ; Lee Ki-Teak ; Kwak Sang-Soo ; Lee Haeng-Soon ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 209~215
DOI : 10.5010/JPB.2005.32.3.209
Lactoferrin is an iron-binding glycoprotein with many biological roles, including the protection against microbial and virus infection, stimulation of the immune system. We developed the transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing the human lactoferrin (hLf) protein following Agrobacterium tumefaciens-mediated transformation. A construct containing a targeting signal peptide from tobacco endoplasmic reticulum fused to hLf cDNA under the control of an oxidative stress-inducible SWPA2 promoter was engineered. Transgenic Siberian ginseng cultured cells to produce a recombinant hLf protein were successfully generated and confirmed by PCR and Southern blot analysis. ELISA and western blot analysis showed that full length-hLf protein was synthesized in the transgenic cells. The production of hLf increased proportionally to cell growth and reached a maximal (up to 3% of total soluble proteins) at the stationary phase. These results suggest that the transgenic Siberian ginseng cultured cells in this study will be biotechnologically useful for the commercial production of medicinal plant cell cultures to produce hLf protein.
In vitro Culture of Adventitious Roots from Dioscorea nipponica Makino for the Production of Steroidal Saponins
An Jung-Hee ; Son Kun-Ho ; Sohn Ho-Yong ; Kwon Soon-Tae ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 217~223
DOI : 10.5010/JPB.2005.32.3.217
Effects of growth regulators on growth of adventitious roots and accumulation of steroidal saponins, such as dioscin, prosapogenin A and prosapogenin C, in cultures of Dioscorea nipponica were determined. The maximum growth of adventitious roots was observed in MS medium supplemented with 30 g/L sucrose and 1.0 mg/L NAA. Addition of BA in combination with NAA appeared to be no effective in the growth of adventitious roots. Among the twenty different adventitious roots formed from different seeds, strain No. 10 was selected based on production ability of dioscin, and its stability through the successive liquid culture. During the first 4 weeks of incubation, contents of steroidal saponins in adventitious roots were negligible but the contents were markedly increased at 5 weeks of incubation. Dioscin and prosapogenin C content in IBA-treated adventitious roots were significantly higher than those in NAA-treated roots. However, content of prosapogenin A was not significantly different among NAA or IBA level. Results provide that liquid culture of adventitious roots of D. nipponica have a potential for mass production of dioscin including prosapogenin A and prosapogenin C.
Increasement of Secondary Metabolites and Antioxidative Activity in Panax ginseng Adventitious Root by Methyl Jasmonate
Lim Soon ; Bae Ki-Hwa ; Shin Cha-Gyun ; Kim Yoon-Young ; Kim Yun-Soo ;
Journal of Plant Biotechnology, volume 32, issue 3, 2005, Pages 225~231
DOI : 10.5010/JPB.2005.32.3.225
This study was initiated to investigate the impacts of methyl jasmonate (MeJA) on adventitious root growth of Panax ginseng, the production of secondary metabolites, such as ginsenosides and phenolic compounds, and antioxidative activity. Among various concentrations of MeJA,
MeJA increased the ginsenosides accumulation to 26.6 mg/g dry wt, about 8 times higher than the control in ginseng adventitious roots (GAR). In addition,
MeJA increased the accumulation of phenolic compounds to 0.38 mg/g dry wt, about 3 times higher than control in GAR. This MeJA treatment was more effective in conditioned medium (CM) which obtained in bioreactor after 40 days of culture than in fresh medium (FM). Treatment of
MeJA in CM increased the accumulation of ginsenosides (1.7 times) and phenolic compounds (1.2 times) more than in FM, respectively. Consequently, these high accumulation of ginsenosides and phenolic compounds by MeJA led to increase the antioxidative activities expressed to the DPPH scavenging activity (over
). The DPPH scavenging activity in control was