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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
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Volume & Issues
Volume 32, Issue 4 - Dec 2005
Volume 32, Issue 3 - Sep 2005
Volume 32, Issue 2 - Jun 2005
Volume 32, Issue 1 - Mar 2005
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Recent Studies on the Edible Plant Vaccine for Prophylactic Medicine against Microorganism-Mediated Diseases
Hahn Bum-Soo ; Jeong Young-Jae ; Roh Kyung-Hee ; Park Jong-Sug ; Cho Kang-Jin ; Kim Yong-Hwan ; Kim Jong-Bum ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 233~241
DOI : 10.5010/JPB.2005.32.4.233
Plants have considerable advantages for the production of antigenic proteins because they provide an inexpensive source of protein and an easy administration of vaccine. Since a publication describing edible plant vaccine of HBsAg in 1992, a number of laboratories around the world have studied the use of plants as the bioreactor to produce antigenic proteins of human or animal pathogens. Over the last ten years, these works have been mainly focused on three major strategies for the production of antigenic proteins in plants: stable genetic transformation of either the nuclear or plastid genome, or transient expression in plants using viral vectors. As many antigenic proteins have been expressed in tobacco, also several laboratories have succeeded to express genes encoding antigenic proteins in other crop plants: potato, tomato, maize, carrot, soybean and spinach. At present many works for the production of edible plant vaccine against bacteria-mediated diseases have mostly performed the studies of enterotoxins and adhesion proteins. Also the development of new-type antigens (pili, flagella, surface protein, other enterotoxin and exotoxin etc.) is required for various targets and more efficacy to immunize against microorganism pathogens. Many works mostly studied in experimental animals had good results, and phase I clinical trial of LTB clearly indicated its immunogenic ability. On the other hand, edible plant vaccines have still problems remained to be solved. In addition to the accumulation of sufficient antigen in plants, human health, environment and agriculture regulation should be proven. Also oral tolerance, the physiological response to food antigens and commensal flora is the induction of a state of specific immunological unresponsiveness, needs to be addressed before plant-derived vaccine becomes a therapeutic option.
Cloning of Coat Protein Gene from Korean Isolate Potato Leafroll Virus (PLRV) and Introduction into Potato (Solanum tuberosum)
Seo Hyo-Won ; Yi Jung-Yoon ; Park Young-Eun ; Cho Ji-Hong ; Hahm Young-Il ; Cho Hyun-Mook ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 243~250
DOI : 10.5010/JPB.2005.32.4.243
The coat protein gene (AF296280) of the Korean isolate Potato leafroll virus (PLRV) was cloned and the open reading frame (627 bp) was transformed into potato (Solanum tuberosum cv. Superior). Out of seventeen individual transgenic lines, five lines were identified to confer resistance to PLRV through the five generation's selection program in the greenhouse as well as isolated trial field. Successful introduction and genetic stability of coat protein gene in the genome of potato were confirmed by polymerase chain reaction (PCR), Southern blot hybridization and northern blot hybridization. Some of the transgenic lines were highly resistant to PLRV but did not show any resistance to less homologous Potato virus Y (PVY). Our results suggest that the resistance to PLRV is due to homology dependent gene silencing by sense strand coat protein gene. In addition, the results of field test through five generations showed that there were no significant differences comparing to nontransgenic potatoes in the morphological aspect of shoot as well as tuber, Ho remarkable differences were also observed in the major agronomic characters and yields except for the resistance to PLRV.
Proteomic Analysis of Cytokinin Induced Proteins in Arabidopsis
Liang Ying-Shi ; Cha Joon-Yung ; Ermawati Netty ; Jung Min-Hee ; Bae Dong-Won ; Lee Chang-Won ; Son Dae-Young ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 251~256
DOI : 10.5010/JPB.2005.32.4.251
Cytokinins are essential plant hormones that play crucial roles in various aspects of plant growth and development. To better understand the molecular mechanisms of cytokinin action, we identified cytokinin related proteins by a proteomic approach. Proteins extracted from control and trans-zeatin treated Arabidopsis seedlings were separated and analyzed by two dimensional gel analysis. Differentially expressed protein spots were identified with peptide mass fingerprinting based on matrix assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and database searching, We obtained ten up-regulated and one down-regulated proteins upon t-zeatin treatment. The expression of the following proteins was induced; pollen allergen like protein, L-ascorbate peroxidase, tetrapyrrole methylase family protein, SGT1 protein homolog, disease resistance related protein, maternal embryogenesis control protein, paxneb related protein, gluthathione S-transferase and IAA amino acid hydrolase homolog.
Production of Transgenic Melon from the Cultures of Cotyledonary-Node Explant Using Agrobacterium-Mediated Transformation
Cho Mi-Ae ; Song Yun-Mi ; Park Yun-Ok ; Ko Suck-Min ; Min Sung-Ran ; Liu Jang-Ryol ; Lee Jun-Haeng ; Choi Pil-Son ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 257~262
DOI : 10.5010/JPB.2005.32.4.257
Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic melon. Cotyledonary-node explants of melon (Cucumis melo L. cv. Super VIP) were co-cultivated with Agrobacterium strains (LBA4404, GV3101, EHA101) containing the binary vector (pPTN289) carrying with CaMV 35S promoter-gus gene as reporter gene and NOS promoter-bar gene conferring resistance to glufosinate (herbicide Basta) as selective agent, and the binary vector (pPTN290) carrying with Ubiquitin promoter-GUS gene and NOS promoter-nptll gene conferring resistance to paromomycin as selective agent, respectively. The maximum transformation efficiency (0.12%) was only obtained from the cotyledonary-node explants co-cultivated with EHA101 strain (pPTN289) on selection medium with 5 mg/L glufosinate and not produced a transgenic melon from the cotyledon or cotyledonary-node co-cultivated with other strains. Finally, five plants transformed showed the resistance in glufosinate antibiotic and the GUS positive response in leaf (
), flower (
), seeds (
) and plantlet (
). Southern blot analysis revealed that the gus gene integrated into each genome of transgenic melon.
Transgenic Sweetpotato (Ipomoea batatas) Expressing Spike Gene of Porcine Epidemic Diarrhea Virus
Yang Kyoung-Sil ; Lim Soon ; Kwon Suk-Yoon ; Kwak Sang-Soo ; Kim Hyun-Soo ; Lee Haeng-Soon ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 263~268
DOI : 10.5010/JPB.2005.32.4.263
Porcine epidemic diarrhea virus (PEDV) causes acute enteritis in pigs of all ages and is often fatal for neonates. In order to develop sweetpotato plants expressing PEDV antigen, we constructed the vector expressing spike gene of PEDV under the control of sweetpotato sporamin promoter or constitutive CaMV 35S promoter. The spike protein region of PEDV was synthesized by PCR and linked to each promoter, Transgenic sweetpotato [Ipomoea batatas (L.) Lam. cv. Yulmi] plants were developed from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. The co-cultured embryogenic calli transferred to selective MS medium containing 1 mg/L 2,4-D, 100 mg/L kanamycin, and 400 mg/L claforan. These embryogenic calli were subcultured to the same selection medium at 3 weeks interval. Kanamycin-resistant calli transferred to hormone-free MS medium with kanamycin gave rise to somatic embryos and then converted into plantlets in the same medium. Southern blot analysis confirmed that the spike gene of PEDV was inserted into the genome of the sweetpotato plants. RT-PCR revealed that the spike gene of PEDV was highly expressed in transgenic sweetpotato plants.
Expression and Purification of Toll-like Receptor 9 Cytoplasmic Domain in Pichia patoris
Lee Kyun-Young ; Lee Kon-Ho ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 269~273
DOI : 10.5010/JPB.2005.32.4.269
Toll-like receptors (TLR) are important components of innate immunity in the defense against pathogens. TLRs recognize pathogen-associated common molecular patterns. TLRs are similar to the receptors involved in defense responses in plants. TLR protein is a type 1 membrane protein, consisting of an extracellular domain containing leucine-rich repeats and a cytoplasmic domain. The cytoplasmic domain delivers ligand recognition signals that result in production of anti-microbial agents. The cytoplasmic domain (amino acid 858-1032) of toll-like receptor 9 has been expressed using methylotrophic yeast Pichia pastoris. The protein expression was confirmed by Western-blot, N-terminal sequencing and MALDl-TOF mass spectrometry. The proteins have been purified by nickel affinity, cation exchange and gel-filtration chromatography.
Quantitative Analysis of Transient Expression in Tah Tasai Chinese Cabbage (Brassica campestris var. narinosa) Seedlings Following Agrobacterium-Mediated Transformation
Shin Dong-Il ; Park Hee-Sung ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 275~279
DOI : 10.5010/JPB.2005.32.4.275
Tah tasai chinese cabbage (Brassica campestris var. narinosa), a vegetable plant popularly consumed as several-days-old seedlings in oriental countries, can be easily cultivated using a simple appliance. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration (agroinfiltration) resulted in a successful transient GUS gene expression in tah tasai chinese cabbage seedlings. Pre-germinated seeds were found to be more susceptible to Agrobacterium infection than one-day-old or two-days-old seedlings. We also demonstrated that hydrogen peroxide (HPO) treatment increased GUS expression especially for two-days-old seedlings. In ELISA using seedlings transformed with hepatitis B surface antigen (HBsAg) DNA by agroinfiltration, HBsAg protein synthesis increased more than two folds by HPO treatment to two-days-old seedlings in comparison to the mock-treated pre-germinated seeds.
In vitro Mass Propagation of Ardisia pusilla DC.
Kang Gwan-Ho ; Oh Owel-Sun ; Goo Dae-Hoe ; Eun Jong-Seon ; Kim Hyung-Moo ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 281~285
DOI : 10.5010/JPB.2005.32.4.281
To establish the mass proliferation system of Ardisia pusilla DC, the shoot tips of Ardisia pusilla DC were cultured on the MS and half-strength MS medium supplemented with
mg/L BA or
mg/L thidiazuron(TDZ), respectively. A few multiple shoot formation observed when the shoots were cultured on MS medium containing TDZ. However, the frequency of multiple shoot formation was reached up to 82.4%, when the shoots were cultured on half-strength MS medium supplemented with 0.5 mg/L BA. Also the number of shoot per explant was 7.1. To promote rooting from multiple shoot, newly formed shoots were transferred to half-strength MS medium containing 0.5 mg/L IBA or 0.5 mg/L NAA, respectively. Regenerated plantlets were grown to normal mature plants in soil.
Plant Regeneration from Cell Suspension Culture Using Leaf Callus in Actinidia deliciosa X A. arguta Clone 118
Kim Yong-Wook ; Moon Heung-Kyu ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 287~292
DOI : 10.5010/JPB.2005.32.4.287
Calli were induced by culturing the leaf segment of Actinidia deliciosa
A. arguta clone 118 on MS medium supplemented with 0.5 mg/L 2,4-D, 0.1 mg/L NAA and 0.05 mg/L BA for 8 weeks in light condition. The induced calli were inoculated in liquid MS medium containing 0.5 mg/L 2,4-D, 0.1 mg/L NAA, 0.05 mg/L BA and 3% sucrose to establish cell suspension culture. The cells at the exponential stage and the stationary stage could be observed between 5-11 days and after that 12 days in culture, respectively. The fresh weight of callus induced from the suspended cells did not vary much among the media containing eight different combinations of plant growth regulators tested. The highest frequency of shoot induction (88.3%) was observed in MS medium containing 2.0 mg/L zeatin. Either BA or zeatin mixed with thidiazuron (TDZ) seemed to be effective in shoot induction. The induced shoots were transferred to MS medium containing 0.2 mg/L zeatin for further shoot growth. And then the shoots were transferred to Standardi (ST) medium containing 1.0 mg/L indolebutyric acid (IBA) for rooting. Plantlets could be obtained through cell suspension culture of Actinidia deliciosa
A. arguta clone 118.
The Role of Nitric Oxide on the Growth Regulation of Chinese Cabbage (Brassica campestris L.) Primary Leaves
Ham Jeong-Hun ; Jin Chang-Duck ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 293~300
DOI : 10.5010/JPB.2005.32.4.293
The possible role of nitric oxide (NO)-induced cell division was investigated to explain the physiologycal effects of a NO donor, sodium nitroprusside (SNP) on the growth of primary leaves in chinese cabbage seedling plants. Exogenous treatment of SNP to chinese cabbage plants for 8 days at different concentrations (0, 200, 500 and 1000
) affected the leaf growth in a concentration-dependent manner, showing a maximum growth at
. In accordance with leaf growth responses, the chlorophyll and soluble protein contents increased strongly to 142% and 134% of control at
SNP, respectively. However, a very little decrease in chlorophyll and a 13%> decrease in protein were observed at
SNP. In addition, the content of DNA and RNA also increased maximumly to 142% and 139% of the control at
SNP, respectively, whereas they decreased to 80% and 84% of the control at
SNP. With respect to the development of enzymes related to cell wall synthesis,
SNP led to the maximum activities in both phenylalanine ammonia-lyase (212% of the control) and guaiacol peroxidase (134% of the control). However, the activities of both enzymes were not modified significantly at
SNP. In conclusion, these results suggest that the enhancement of leaf growth in chinese cabbage plants by SNP at the effective concentration was probably due to the NO ability in the induction of cell division.
Shoot Proliferation and Plant Regeneration from Suspension-Cultured Cells of Dianthus gratianopol
Kim Joon-Chul ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 301~306
DOI : 10.5010/JPB.2005.32.4.301
Conditions for efficient organogenesis and plant regeneration from Dianthus gratianopol suspension cultured cells were established. Shoot-forming calli of glossy surface, pale green and knobby type were selected from leaf explant-derived calli and were suspension-subcultured every week in CP liquid medium with 1.0 mg/L 2,4-D and 0.5 mg/L BAP. Combinations of 1.0 mg/L 2,4-D and 0.5 mg/L BAP, and 1.5 mg/L 2,4-D and 0.5 mg/L BAP were effective for the induction of regenerative callus from the suspension cultured cell clusters. Multiple shoot primordia were initiated from the green spots of these regenerative callus and formed shoots on MS medium with 1.0 mg/L TDZ and 0.5 mg/L PAA. Shoot regeneration frequency (calli regenerating at least one shoot) was about 87%. For plant regeneration, proliferated shoots were excised and transferred to MS medium with 0.1 mg/L NAA for root initiation after 9 weeks of culture. The regenerants were potted in soil and formed the flowering buds and petals. Also, adventitious shoots were formed from the excised green shoot primordia of regenerative callus and these shoots proliferated successfully and regenerated to whole plants.
Effect of Light on Anthocyanin Biosynthesis in Callus Culture of Purple Sweetpotato
Park Hyae-Jeong ; Kim Jung-Suk ; Park Hyeon-Yong ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 307~311
DOI : 10.5010/JPB.2005.32.4.307
The anthocyanin biosynthesis in callus culture of purple sweet potato (Ipomoea batatas L. cv. Borami) was investigated under growth in different light intensity and light emitting diodes (LED) treatment. Pigmented calli were induced from leaf explants cultured on MS agar medium supplemented with
2,4-D under light condition. The color value of these calli extracted after
weeks of cultures was
. Irradiation intensity is an important factor for anthocyanin biosynthesis. The optimal anthocyanin accumulation occurred on light intensity of 3000 lux. Light irradiation of 3000 lux and blue light treatment for 2 h resulted in a significant enhancement of anthocyanin accumulation. This value was 1.4 fold that the control.
Effect of Various Carbon Sources on the Production and Stabilization of hGM-CSF in Transgenic Plant Suspension Culture
Lee Jae-Hwa ;
Journal of Plant Biotechnology, volume 32, issue 4, 2005, Pages 313~319
DOI : 10.5010/JPB.2005.32.4.313
The effects of various carbon sources on the secretion of hGM-CSF, total protein and protease into the medium were investigated in transgenic tobacco cells. The dry cell weight (11.2 g/L) and wet cell weight (310.8 g/L) were highest at 30 g/L glucose after 5-day culture but, the dry cell weight (13.4 g/L) and wet cell weight (480 g/L) were highest at 30 g/L sucrose after 10-day culture. The total protein (110.3 mg/L), protease activity (3950 U/L) and total secreted hGM-CSF (56 mg/L) were highest at 30 g/L sucrose after 10-day culture. Stabilization of the total secreted protein and hGM-CSF in various carbon source concentrations was determined. Total secreted protein was most stabilized in the medium containing sucrose. However, the loss of the total protein was increased with the concentrations of high level in medium containing sorbitol, mannitol, fructose, and glucose. hGM-CSF was more stabilized in the medium containing sucrose than in the medium containing sorbitol, mannitol, fructose, glucose.