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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 33, Issue 4 - Dec 2006
Volume 33, Issue 3 - Sep 2006
Volume 33, Issue 2 - Jun 2006
Volume 33, Issue 1 - Mar 2006
Selecting the target year
Production of Human Serum Albumin in Chloroplast-Transformed Tobacco Plants
Ko, Suk-Min ; Kim, Hyun-Chul ; Yoo, Byung-Ho ; Woo, Je-Wook ; Chung, Hwa-Jee ; Choi, Dong-Woog ; Liu, Jang-R. ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 233~236
DOI : 10.5010/JPB.2006.33.4.233
Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5`UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.
Somatic Embryogenesis and Plant Regeneration in Barley (Hordeum vulgare L.)
Park, Tae-Il ; Um, Mi-Ok ; Kim, Young-Jin ; Hwang, Jong-Jin ; Choi, Kyung-Gu ; Yun, Song-Joong ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 237~242
DOI : 10.5010/JPB.2006.33.4.237
Commercial cultivars and elite germplasms of barely (Hordeum vulgare L) are still recalcitrant to genetic transformation because of the lack of an efficient regeneration system. In this study, we established an efficient plant regeneration procedure from embryogenic calli derived from mature embryos. Callus induction from germinated mature embryos was best as over 95% in CIM medium (CI medium containing
dicamba) under dark incubation. Development of embryogenic callus was highest as over 50% in CI3D medium (EC medium supplemented with
2,4-D). The highest regeneration of plants from embryogenic callus (40%) was obtained with CIS medium (
). These plant regeneration conditions could be useful in improving barley transformation efficiency.
Micropropagation of Tilia amurensis via Repetitive Secondary Somatic Embryogenesis
Kim, Tae-Dong ; Choi, Yong-Eui ; Lee, Byoung-Sil ; Kim, Young-Joung ; Kim, Tae-Su ; Kim, In-Sik ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 243~248
DOI : 10.5010/JPB.2006.33.4.243
A optimal procedure for plant production via repetitive secondary somatic embryogenesis in Tilia amurensis is described. Somatic embryos were induced directly from the culture of zygotic embryos on medium with 1.0 mg/l 2,4.-D. Repetitive secondary somatic embryos formed on the surface of the cotyledons and hypocotyls except for the radicles when explants of somatic embryos were cultured on medium with 4.0 mg/l 2,4-D. The highest frequency of secondary embryo-genesis was obtained in the cotyledons (90%) and hypocotyls(83.33%) on MS medium with 1.0 mg/L 2,4-D. The average number of secondary embryos per explant was 25.74 in cotyledon and 24.92 in hypocotyl. When the cotyledon and hypocotyl segments from somatic embryos at different developmental stages were cultured on MS medium with 1.0 mg/L 2,4-D, the highest frequency of secondary embryogenesis was obtained from late cotyledonary secondary embryos. Somatic embryos were transferred to MS basal medium and then they germinated within 2 to 4 weeks of culture. Germinated somatic embryos grew normally into plantlets on WPM medium, producing new shoots. The converted plantlets were acclimatized on artificial soil mixture. These results indicate that the repetitive secondary somatic embryogenesis in T amurensis can offer the possibility to use in vitro culture system for the micropropagation.
Effect of Ethylene Inhibitors on In Vitro Shoot Multiplication and their Impact on Ethylene Production in Cucumber (Cucumis sativus L.)
Vasudevan A. ; Selvaraj N. ; Ganapathi A. ; Anbazhagan V. Ramesh ; Choi, C.W. ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 249~255
DOI : 10.5010/JPB.2006.33.4.249
Effects of ethylene inhibitors like silver nitrate
, cobalt chloride
and Salicylic acid (SA) on multiple shoot induction and their impact on ethylene production using embryonal cotyledon cultures of Cucumis sativus L. were examined. The optimum concentration of
, separately, induced maximum number of shoots on Murashige and Skoog`s (MS) medium supplemented optimally with
NAA. Among the three ethylene inhibitors tested,
produced maximum number of shoots when compared to
and SA Ethylene production was monitored in all the treatments with
and it was observed that the treatment with
alone showed increase in ethylene production when compared to
and SA Even though ethylene concentration was the highest in
treated explants, maximum number of shoots was obtained.
High Frequency Somatic Embryogenic Callus Induction and Plant Regeneration from Various Indica Rice Genotypes
Hoque Md. Enamul ; Mansfield John W. ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 257~262
DOI : 10.5010/JPB.2006.33.4.257
The paper evaluated the behavior of in vitro culture responses from a diverse set of Indica rice (Oryza sativa L.) genotypes. Significant differences were found in embryogenic callus induction frequency, callus growth and plant regeneration frequency when mature embryos of 11 cultivars, breeding lines and land races were compared. Genotype as well as plant growth regulator influenced the plant regeneration frequency. Callus induction frequency was not correlated with callus growth as well as plant regeneration frequency. The regenerated plants could grow to normal, fertile plants after they were successfully established in soil.
In Vitro Regeneration of Pongamia pinnata Pierre
Sujatha, K. ; Hazra, Sulekha ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 263~270
DOI : 10.5010/JPB.2006.33.4.263
Pongamia pinnata Pierre is a tree legume, having potential in production of raw material for biodiesel. A protocol for in wk propagation of this plant was standardized using seedling explants. Growth regulators (GR) including gibberellic acid
, thidiazuron (TDZ), and Adenine sulphate (Ads) were tested for optimum germination of seeds. Removal of seed coat prior to germination, controlled fungal growth partially but enhanced bacterial growth. Antibiotic cefotaxime was ineffective in controlling bacterial contamination. Seedling derived nodal explants and cotyledon nodes with attached cotyledons were excised and cultured for induction of shoots. Optimum sprouting and multiplication of shoot buds were obtained in MS medium supplemented with
BA. These buds differentiated and rooted on medium devoid of GR. Optimum growth of Pongamia seedling was obtained in cotton plugged culture vessels. Reculturing of the cotyledon node explants produced more shoots from the same site. This process of removing shoots and reculturing of cotyledon node was followed for eight passages yielding 4 to 8 shoots in each cycle. The shoots (75%) rooted on half strength MS basal medium supplemented with 0.22% charcoal. All plants survived on transfer to soil. This is the first report on in vitro regeneration of Pongamia pinnata. This report demonstrates the possibility of coupling more than one parameter in single experiment to hasten the process of standardization. The process of cycling the nodal explant repeatedly for production of large number of shoots from single meristem may find application in genetic transformation experiments wherein meristems are used for transformation.
Use of Paromomycin as a Selectable Marker for the Transformation of Chinese Cabbage
Cho, Mi-Ae ; Min, Sung-Ran ; Ko, Suck-Min ; Liu, Jang-Ryol ; Lee, Jun-Haeng ; Choi, Pil-Son ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 271~276
DOI : 10.5010/JPB.2006.33.4.271
Hypocotyl explants of Chinese cabbage (us. `Jeong Sang` and `Seoul`) produced adventitious shoots on Murashige and Skoog (MS) basal medium supplemented with 4mg/L
, 5 mg/L acetosyringone, 4 mg/L 6-benzyladenine and 3mg/L alpha-naphthaleneacetic acid (SI) after cocoultivation with strains of Agrobacterium tumefaciens (LBA4404) harboring the pCAMBIA1301 and the
containing hygromycin-resistance gene and paromomycin-resistance gene as a selectable marker genes, respectively. There was a significant difference in the frequency of transgenic plants depending on antibiotics and cultivars used. Paromomycin was better than hygromycin, and cultivar `Jeong-sang` was higher than `c.v. Seoul` in the frequency of transgenic plants. In particular, the highest frequency (0.70%) of transgenic plants was obtained from selection medium (SI) containing 100mg/L paromomycin in c.v., `Jeong-sang` GUS positive response were obtained 9 plants and 3 plants from the cultivars, `Jeong-sang` and `Seoul`, respectively. They were grown to maturity in a greenhouse and normally produced
seeds. GUS histochemical assay for progeny
revealed that the transgenes were expressed in the plant genome.
Feasibility of Determining the Ripeness of Strawberry Fruit Flesh by Fourier Transform Infrared Spectroscopy
Min, Sung-Ran ; Kwak, Chul-Won ; Kim, Suk-Weon ; Jeong, Won-Joong ; Chung, Hwa-Jee ; Choi, Pil-Son ; Ko, Suk-Min ; Park, Sang-Kyu ; Chung, Hoe-Il ; Liu, Jang, R. ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 277~281
DOI : 10.5010/JPB.2006.33.4.277
Fourier transform - infrared spectroscopy (FT-IR) provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when whole cell extracts are analyzed. We attempted to determine the ripeness of strawberry fruit flesh by FT-IR. Fruit ripeness was divided into four developmental stages based on fruit skin color: `yellow-green`, `pink-green`, `pink`, and `red` stages. Principal component analysis of FT-IR data of inside fruit flesh extracts clustered samples of four different developmental stages into three discrete groups: (1) `yellow-green` group, (2) `pink-green` group, and (3) `pink` and `red` group. The most remarkable difference between four different developmental stages was found in the carbohydrate fingerprint region
of the FT-IR spectrum, indicating that differences in carbohydrate compounds represented the ripeness of strawberry fruit. Overall results indicate that FT-IR in combination with PCA enables discrimination of the ripeness of strawberry fruit flesh.
Metabolic Discrimination of Rice Cultivars and Relative Quantification of Major Sugar Compounds Using
H NMR Spectroscopy Combined by Multivariate Statistical Analysis
Kim, Suk-Weon ; Koo, Bon-Cho ; Kim, Jong-Hyun ; Liu, Jang-Ryol ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 283~288
DOI : 10.5010/JPB.2006.33.4.283
Discrimination of 5 rice cultivars (Sangjubyeo
, and Simbaek-hetero
) using metabolic profiling was carried out. Whole cell extracts from each cultivar were subjected to
NMR spectroscopy. When spectral data were analyzed by principal component analysis, 5 cultivars were clustered into 3 groups: SJ, DJ + SB, and HM + SH. Thecultivars showed great difference in carbohydrate region of
NMR spectra, suggesting that qualitative and quantitative differences in carbohydrate compounds play a major role in discrimination of the cultivars. In addition, it was readily possible to determine relative quantification of major carbohydrates including sucrose, glucose, maltose from spectral data of the cultivars. SJ showed 2 to 4 times higher content of maltose than the other rice cultivars. Overall results indicate that metabolic discrimination of rice cultivars using
NMR spectroscopy combined by multivariate statistical analysis can be used for rapid discrimination of numerous rice cultivars and simple quantitative analysis system of major carbohydrate compounds in rice grains.
Use of Simple Sequence Repeat (SSR) Markers for Variety Identification of Tomato (Lycopersicon esculentum)
Kwon, Yong-Sham ; Park, Eun-Kyung ; Bae, Kyung-Mi ; Yi, Seung-In ; Park, Soon-Gi ; Cho, Il-Ho ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 289~295
DOI : 10.5010/JPB.2006.33.4.289
This study was carried out to evaluate the suitability of simple sequence repeat (SSR) markers for varietal identification and genetic diversity in 28 commercial tomato varieties. The relationship between marker genotypes and 28 varieties was analyzed. Of the 219 pairs of SSR primers screened against ten tomato varieties, 18 pairs were highly polymorphic with polymorphism information content (PIC) ranging from 0.467 to 0.800. Among the polymorphic loci, two to nine SSR alleles were detected for each locus with an average of 3.3 alleles per locus. Genetic distances were estimated according to Jaccard`s methods based on the probability that the amplified fragment from one genotype would be present in another genotype. These varieties were categorized into cherry and classic fruit groups corresponding to varietal types and genetic distance of cluster ranging from 0.35 to 0.97. The phonogram discriminated all varieties by marker genotypes. The SSR markers proved to be useful variety identification and genetic resource analysis of tomato.
Endoreduplication Pattern of Somatic Embryos and Variants Occurrence Affected by Pre-existed Endoreduplicated Cells in Doritaenopsis
Park, So-Young ; Paek, Kee-Yoeup ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 297~302
DOI : 10.5010/JPB.2006.33.4.297
In general, the proliferation of orchids via somatic embryos has been used for mass production of somatic clones because of high propagation efficiency. In spite of high propagation rate, this method often brings somaclonal variation, especially polyploid frequency. Therefor we here concentrated to investigate the relationship between endopolyploidization patterns of explants and the occurrence of tetraploid variant in clonally proliferated Doritaenopsis via somatic embryo regeneration system. In the fully developed somatic embryo, upper part contained 2C to 16C while middle and lower parts showed 2C to 32C DNA content. Two-week-old embryo contained 2C to 16C, whereas those regenerated after 4 to 10-week-old contained 2C to 64C nuclei. Results showed that endoreduplication was variable depending upon tissue types, ages, and parts in one species. lower part of somatic embryo having high endoreduplication degree increased the regeneration of tetraploid variants by about 3-fold comparing to upper part of somatic embryo culture. polyploid frequency occurrence might be closely related to the high levels of endoreduplication of somatic embryos used as explant. It suggested that the upper part of somatic embryo having comparatively low endoreduplication degree is suitable for the stable in vitro propagation system.
Multiple Shoot Induction from Radicle-derived Callus and in Vitro Propagation of Silene Acaulis Subsp. Arctics
Seo, Hyo-Won ; Yi, Jung-Yoon ; Park, Young-Eun ; Kang, Sung-Ho ; Chung, Ho-Sung ; Kim, Ji-Hee ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 303~307
DOI : 10.5010/JPB.2006.33.4.303
We describe here an efficient in vitro propagation method of Silene acaulis subsp. arctica (Caryophyllaceae), one of the higher arctic angiosperms, through the multiple shoot regeneration after callus induction from the radicle. The seeds of S. acaulis subsp. arctica collected from Svalbard, the Norwegian Arctic, were germinated and calli were induced from the radicle on solid MS media supplemented with 0.25mg/L 2,4-D and 1mg/L
Two weeks after callus induction, the multiple shoots were efficiently regenerated on the MS media supplemented with 0.25 g/L BA and 0.05mg/L HPh. The total biomass increment of regenerated shoots increased most efficiently of S. acaulis subsp. afctica was showed the maximum efficiency in at
on 1/2 MS salt strength. The multiple regenerated plantlets of S. acaulis subsp. arctics were grown to normal plants on soil.
Improvement of Selection Efficiency for Bacterial Blight Resistance Using SNP Marker in Rice
Shin, Woon-Chul ; Baek, So-Hyeon ; Seo, Chun-Sun ; Kang, Hyeon-Jung ; Kim, Chung-Kon ; Shin, Mun-Sik ; Lee, Gang-Seob ; Hahn, Jang-Ho ; Kim, Hyun-Soon ;
Journal of Plant Biotechnology, volume 33, issue 4, 2006, Pages 309~313
DOI : 10.5010/JPB.2006.33.4.309
Discovery of single nucleotide polymorphisms (SNPs), including small insertions and deletions, is one of the hot topics in genetic research. The most common type of sequence variant consists of single base differences or small insertions and deletions at specific nucleotide positions. Significance of SNPs in rice is increasing for genetic research, positional cloning and molecular breeding.
170 lines and
194 lines derived from Sangjuchalbyeo/HR13721-53-3-1-3-3-2-2 Were used for Searching SNP markers related to bacterial blight resistance. Sangjuchalbyeo is susceptible to bacterial blight, but HR13721-53-3-1-3-3-2-2 has Xa1 gene resistant to bacterial blight. Individual lines were inoculated with
race of bacterial blight and resistant or susceptible was evaluated after 3 weeks from inoculation. The genotypes of population were analysed by PCR-RFLP for SNP marker developing. The segregation of
population showed almost 3:1, 1:1 ratio, respectively. Analysis of genotype using SNP marker is capable of confirming resistance for
race and genotype through amplifying the gene using 16PFXal primer and digested the PCR product with Eco RV. There were close relation between resistance test for
race and SNP marker genotype. Especially, DNA analysis using SNP marker is capable of judging homozygote/heterozygote in
population compared with resistant test for Kl race. So, it seems to improve the selection efficiency in disease resistant breeding.