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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 34, Issue 4 - Dec 2007
Volume 34, Issue 3 - Sep 2007
Volume 34, Issue 2 - Jun 2007
Volume 34, Issue 1 - Mar 2007
Selecting the target year
Effect of BA Concentrations and Culture Methods on in Vitro Plant Multiplication from Shoot-Tip Culture of Wasabia japonica
Park, Yun-Young ; Cho, Moon-Soo ; Lee, Young-Deuk ; Chung, Jong-Bae ; Park, Shin ; Jeong, Byeong-Ryong ; Park, Sang-Gyu ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 1~6
DOI : 10.5010/JPB.2007.34.1.001
Effect of BA concentrations and culture methods on in vitro plant multiplication from shoot-tip cultures of Wasabia japonica was studied. Shoot-tips with leaf primordia and apical meristem were cultured on MS basal medium for all the experiments. Liquid medium for 2 weeks followed by semi-solid medium for 4 weeks containing 1.0 mg/L BA was the best to number of shoots (22.8) and shoot length (3.5 cm). Shoots proliferated could be divided into ca. 5 to 11 of cultures for the multiplication of plantlets. Divided plantlets showed root formation (90%) well onto MS basal medium without growth regulators like IBA and NAA. After rooting, all the plantlets transferred into the pots containing composed soil (bio-media Co., peatmoss
, coir dust
) and grown well into whole plants with multiple shoots.
Plant Regeneration from Floral Stem Cultures of Nymphoides indica (L.) O. Kuntze. via Somatic Embryogenesis
Oh, Myung-Jin ; Min, Sung-Ran ; Liu, Jang-Ryol ; Kim, Suk-Weon ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 7~10
DOI : 10.5010/JPB.2007.34.1.007
Plant regeneration system from floral stem of Mymphoides indica via somatic embryogenesis was established. After four weeks of culture onto 1/2MS medium containing 2,4-D, pale-yellow globular structures and calluses were formed on the cut surface of floral stem explants. Upon transfer to 1/2MS basal medium, pale-yellow globular structures were developed into somatic embryos and normal plantlets. These results indicated that pale-yellow globular structures and calluses from floral stem were globular embryos and embryogenic calluses, respectively. The frequency of embryogenic callus formation from floral stem was reached to nearly 100% when floral stem was cultured onto 1/2Ms medium supplemented with low concentration of 2,4-D (0.1 to 0.3 mg/L). However, the higher concentration of 2,4-D resulted in decrease of the frequency of embryogenic callus formation. In this study, low concentration of 2,4-D had a stimulative role in embryogenic callus formation, whereas BA showed inhibitory role in callus formation. In comparison to floral stem, leaf explants showed low frequency of embryogenic callus formation. The highest frequency of embryogenic callus formation from leaf explants was 9.5% when leaf explants were cultured onto 1/2MS medium supplemented with 0.3 mg/L of 2,4-D. The plant regeneration system of Nymphoides indica established in this study, might be applied to mass proliferation, conservation of genetic resources and genetic transformation for molecular breeding.
CGMMV Tolerance Test of CGMMV-CP Trangenic Watermelon Rootstock and Establishment of Transgenic Line
Park, Sang-Mi ; Kwon, Jung-Hee ; Lim, Mi-Young ; Shin, Yoon-Sup ; Her, Nam-Han ; Lee, Jang-Ha ; Ryu, Ki-Hyun ; Harn, Chee-Hark ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 11~17
DOI : 10.5010/JPB.2007.34.1.011
Previously developed transgenic watermelon rootstocks (gongdae) inserted by CGMMV-CP were examined to test the virus tolerance levels. In the restricted plastic house, the
watermelon rootstock showed tolerance to CGMMV until 70 days after inoculation on the leaves while the non-transformed watermelon rootstock became susceptible at 20 days after inoculation. In the field, tolerance efficiency of transgenic rootstocks maintained up to 40% at 71 days after contamination with CGMMV in the soil while all of the non-transformed rootstocks became susceptible at 37 days with the same condition. In the same field, transgenic rootstocks showed more tolerance to CGMMV than the non-transformed rootstocks as those were inoculated on the leaves, but it showed only 10 days delay before being susceptible. Therefore, transgenic rootstocks have a characteristic of delay effect against CGMMV susceptibility, rather than resistance character. From
rootstocks homozygous for the CGMMV-CP horticulturally favorable individuals were selected for further breeding and a transgenic line was finally obtained at the
. A material transfer experiment was conducted to find out if the DNA, RNA or expressed protein in the transgenic rootstocks could move to the grafted scion (non-transformed watermelon, Super-Kumcheon). PCR, northern, and western blot analysis were performed and no evidence of transferring of those materials from rootstock to scion was ever found.
Selection of High Anthocyanin-Producing Cells from Habituated Callus Derived from Purple Sweetpotato
Park, Hye-Jeong ; Kim, Yoon-Sil ; Park, Hyeon-Yong ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 19~24
DOI : 10.5010/JPB.2007.34.1.019
Callus induction using leaf of purple sweetpotato (PSP) was decreased when subcultured. So we selected habituated callus in MS medium supplemented with
(2,4-dichlorophenoxyacetic acid) after 6 months of cultures (without subculture). It grew faster and easier than any other callus. It was able to proliferate in MS hormone free solid and liquid medium without any growth regulators and subculture limits. During subculture in liquid medium, a purple mottled spot formed in one of habituated cell aggregates without any treatment. This purple cell aggregates were carefully separated from habituated cell aggregates, and then subcultured by selecting purple cell aggregates for more than 2 years to be isolated. The color value of the pigment extracted of culture was 1.0 mg/mL, which was close to that of a pigment extracted from storage root, which was 1.5 mg/mL. This purple cell aggregates could therefore be used for the industrial mass production of anthocyanin.
Plant Regeneration from Zygotic Embryos Cultures of Lilium Lancifolium Thunb. Via Bulblet Formation
Kim, Kyung-Hee ; Liu, Jang-Ryol ; Kim, Suk-Weon ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 25~29
DOI : 10.5010/JPB.2007.34.1.025
Plant regeneration system from zygotic embryos (2n
D-amino Acid Oxidase (DAO) Gene as a Novel Selection Marker for Plant Transformation
Lim, Sun-Hyung ; Woo, Hee-Jong ; Lee, Si-Myung ; Jin, Yong-Moon ; Cho, Hyun-Suk ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 31~36
DOI : 10.5010/JPB.2007.34.1.031
Though higher plants car not metabolize D-amino acid, many prokaryotes and eukaryotes have the D-amino acid metabolism. Therefore, we transformed tobacco plants with D-amino acid oxidase (DAO), which can metabolize D-amino acid, and confirmed that transgenic tobacco plants might metabolize D-amino acid. Transgenic tobacco plants were survived a high concentration of D-serine, however non-transgenic plants were not grown on D-serine medium. From Southern and Northern blot analysis, transgenic tobacco plants selected on D-serine medium were confirmed by insert and expression of transgene.
tobacco seeds derived
tobacco plants selfing were grown on D-serine medium and showed normal phenotype compared to wild tobacco plants. Transgenic tobacco plants displayed the metabolic capability of D-serine. Therefore, we suggested that DAO is useful selectable marker gene for plant transformation.
Genetic Transformation of Watermelon (Citrullus vulgaris Schard.) by Callus Induction
Kwon, Jung-Hee ; Park, Sang-Mi ; Lim, Mi-Young ; Shin, Yoon-Sup ; Harn, Chee-Hark ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 37~45
DOI : 10.5010/JPB.2007.34.1.037
The genetic transformation of watermelon by Agrobacterium has been known very difficult and a few successful cases have been reported by obtaining the direct shoot formation. However, since this direct shoot formation is not guaranteed the stable transformation, the stable transformation with reproducibility is required by a different approach such as a callus induced manner. The best conditions for inducing the callus from cotyledon and root explants of watermelon were 2 mg/L zeatin + 0.1 mg/L IAA and 2 mg/L BA + 0.1 mg/L 2,4-D, respectively. The GFP expression in the callus was identified and monitored through fluorescent microscopy after transformation with pmGFP5-ER vector. Paromomycin rather than kanamycin was used for selecting the nptll gene expression because it was more effective to select the watermelon explants. Four different callus types were observed and the solid green callus showed stronger GFP expression. The highest frequency of GFP expression in the callus developed from cotyledon was 9.0% (WM8 inbred line), while the highest frequency from root was 8.3% (WM6 inbred line). The WMV-CP was transformed using the method of GFP transformation and the genetic transformation of WMV-CP was confirmed by PCR and Southern blot analysis. Here we present a system for callus induction of watermelon explant and the callus induced method would facilitate the establishment of stable watermelon transformation.
Pollen-Mediated Gene Flow between Glufosinate Ammonium-Tolerant GM and Non-GM Rice
Lee, Seung-Yeob ; Kim, Min-Soo ; Kim, Hyo-Jin ; Ahn, Jeong-Ho ; Baek, So-Hyeon ; Shin, Woon-Chul ; Kim, Hyun-Soon ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 47~53
DOI : 10.5010/JPB.2007.34.1.047
To assess the risk of genetically modified (GM) rice on the agricultural ecosystem, agronomic characteristics, pollen longevity and outcrossing rate between GM (Iksan 483 and Milyang 204) and non-GM (their wild types and female parents) varieties were investigated using the bar gene as a tracer marker in paddy field. The agronomic characteristics of two GM rice were similar to their female-parents (non-GM rice) except heading date and 1,000 grain weight of Iksan 483, and they did not show a difference by the introgression of the bar gene as the genetic traits of rice varieties. Pollen viability was more than 90% just after shedding, and it was rapidly decreased below 50% at 5 minutes after shedding both GM and non-GM varieties. The Pollen longevity was lost after 30 minutes of anthesis. When the distance of gene flow from GM to non-GM rice detected to 6 m from the edge of GM rice plant, the maximum distance of pollen dispersal was 4.5m and 3.9m in Iksan 483 and Milyang 204, respectively, and that was increased in order of west, south, east, and north to the dominant wind direction, west-south. Mean outcrossing rate was very low as 0.003 and 0.001% within 1.5 m from the edge of Iksan 483 and Milyang 204, and the GM hybrids by the pollen dispersal did not detected over 4.5 m from the edge of GM rice plant. The results may help to establish the strategy which reduce the risk of pollen-mediated gene flow between GM and non-GM rice.
Agrobacterium-Mediated Genetic Transformation of Pepper for the Development of Blight Resistant Cultivar
Kwon, Tae-Ryong ; Lee, Moon-Jung ; Han, Jung-Sul ; Shin, Dong-Hyun ; Oh, Jung-Youl ; Kim, Kyung-Min ; Kim, Chang-Kil ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 55~59
DOI : 10.5010/JPB.2007.34.1.055
The study was carried out to develop transformants resisting to Phyophthora blight disease in the domestic pepper cultivar Subicho. In transforming of syn600 promoter with elicitin gene using Agrobacterium (LBA4404/pBI101 syn600-syn
-elicitin) to cotyledons of pepper, rate of shoot formation in `Subicho` was 11.1% in medium containing 3 mg/L zeatin and 0.05 mg/L NAA, and also 12.8% in medium containing combination of 4 mg/L zeatin and 0.05 mg/L MAA. For PCR reaction using elicitin gene primer of transformants regenerated from cotyledons, we detected a specific band of 536 bp, and also showed strong signal at position of 536 bp in accordance with NPTII gene used as probe in Southern blot. Transformants pepper shown resistance to blight fungus was inoculated to seedlings of the
transformants by concentration (density: zoo spore
Characterization of Salt Tolerant Rice Mutant Lines Derived from Azetidine-2-Carboxylic Acid Resistant Cell Lines Induced by Gamma Ray Irradiation
Song, Jae-Young ; Kim, Dong-Sub ; Lee, Geung-Joo ; Lee, In-Sok ; Kang, Kwon-Kyoo ; Yun, Song-Joong ; Kang, Si-Yong ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 61~68
DOI : 10.5010/JPB.2007.34.1.061
To develop rice (Oryza sativa L.) cultivars to be planted on salt-affected sites, cell lines with enhanced proline content and resistance to growth inhibition by Azetidine-2-carboxylic acid (AZCA), a proline analogue, were screened out among calli irradiated with gamma ray of 50, 70, 90, and 120 Gy. The calli had been derived from embryo culture of the cultivar Donganbyeo. Selected AZCA resistant lines that had high proline accumulation were used as sources for selection of NaCl resistant lines. To determine an optimum concentration for selection of NaCl resistant lines, Donganbyeo seeds were initially cultured on the media containing various NaCl concentrations (0 to 2.5%) for 40 days, and 1.5% NaCl concentration was determined as the optimum concentration. One hundred sixteen salt-tolerant (ST) lines were selected from bulked 20,000 seeds of the AZCA resistant
seeds in the medium containing 1.5% NaCl. The putative 33 lines (
generation) considered with salt-tolerance were further analyzed for salt tolerance, amino acid and ion contents, and expression patterns of the salt tolerance-related genes. Out of the 33 lines, 7 lines were confirmed to have superior salt tolerance. Based on growth comparison of the entries, the selected mutant lines exhibited greater shoot length with average 1.5 times, root length with 1.3 times, root numbers with 1.1 times, and fresh weight with 1.5 times than control. Proline contents were increased maximum 20%, 100% and 20% in the leaf, seed and callus, respectively, of the selected lines. Compared to control, amino acid contents of the mutants were 24 to 29%, 49 to 143%, 32 to 60% higher in the leaf, seed and callus, respectively. The ratio of
for most of the ST-lines were lower than that of control, ranging from 1.0 to 3.8 for the leaf and 11.5 to 28.5 for the root, while the control had 3.5 and 32.9 in the leaf and root, respectively. The transcription patterns for the P5CS and NHXI genes observed by RT-PCR analysis indicated that these genes were actively expressed under salt stress. The selected mutants will be useful for the development of rice cultivar resistant to salt stress.
High Frequency Plant Regeneration from the Cultures of Cotyledon Explants of Perilla (Perilla frutescens L.)
Kim, Kyung-Min ; Lee, Hyeon-Suk ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 69~73
DOI : 10.5010/JPB.2007.34.1.069
A reliable and effective tissue culture system was established for Perilla frutescens (perilla) using different cultivar, explant source, and growth regulator composition in medium. MS medium supplemented with 2.0 mg/L BA and 0.1 mg/L NAA was maximum at shoot induction. Cotyledon explants formed more shoots than hypocotyl explants. The frequency of plant regeneration through organogenesis was higher (22.8%) than that (6.1%) of somatic embryogenesis. Five genotypes of perilla were screened for the feasibility of shoot regeneration, cotyledon explant of `Manbaek` showed the highest shoot induction at a frequency of 27.3% among the tested cultivars.
Evaluation of horizontal gene transfer from genetically modified zoysiagrass to the indigenous microorganisms in isolated GMO field
Bae, Tae-Wung ; Lee, Hyo-Yeon ; Ryu, Ki-Hyun ; Lee, Tae-Hyeong ; Lim, Pyung-Ok ; Yoon, Pill-Yong ; Park, Sin-Young ; Riu, Key-Zung ; Song, Pill-Soon ; Lee, Yong-Eok ;
Journal of Plant Biotechnology, volume 34, issue 1, 2007, Pages 75~80
DOI : 10.5010/JPB.2007.34.1.075
The release of genetically modified organisms (
) into the environment has the potential risks regarding the possibility of gene transfer from
to natural organisms and this needs to be evaluated. This study was conducted to monitor the possible horizontal gene transfer from herbicide-resistant zoysiagrass (Zoysia japonica Steud.) to indigenous microorganisms. We have first examined the effect of field-released GM zoysiagrass on the microbial flora in the gut of locust (Locusts mlgratoria). The microbial flora was analyzed through determining the 165 rDHA sequences of microorganisms. The comparison of the microbial flora in the gut of locusts that were captured at the field of GM zoysiagrass and of wild-type revealed that there is no noticeable difference between these two groups. This result indicates that the GM zoysiagrass does not have negative impact on microbial flora in the gut of locust. We then investigated whether the horizontal gene transfer occurred from GM zoysiagrass to microbes in soil, rhizosphere and faecal pellets from locusts by utilizing molecular tools such as Southern hybridization and polymerase chain reaction (PCR). When the total DNAs isolated from microbes in GM zoysiagrass and in wild-type zoysiagrass fields were hybridized with probes for bar or hpt gene, no hybridization signal was detected from both field isolates, while the probes were hybridized with DNA from the positive control. Absence of these genes in the FNAs of soil microorganisms as well as microbes in the gut of locust was further confirmed by PCR. Taken together, our data showed that horizontal gene transfer did not occur in this system. These results further indicate that frequencies of transfer of engineered plant DNA to bacteria are likely to be negligible.