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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 35, Issue 4 - Dec 2008
Volume 35, Issue 3 - Sep 2008
Volume 35, Issue 2 - Jun 2008
Volume 35, Issue 1 - Mar 2008
Selecting the target year
Journal of Plant Biotechnology will be revolved toward the most rapid publication in the world plant science community in 2008: from submission to publication within two weeks
Liu, Jang-R. ; Min, Byung-Hoon ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 1~3
DOI : 10.5010/JPB.2008.35.1.001
To revolve the Journal of Plant Biotechnology into the most rapid publication in the world plant science community in 2008 consistent with high standards, we set two plans: reorganization of the Editorial Board with members devoted to the new plan and adoption of e-Journal system, a total solution ensuring from the online submission to publication.
Guideline for managing research facilities and LMOs for R&D by the Act on transboundary movement of LMOs, etc,.
Jang, Ho-Min ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 5~12
DOI : 10.5010/JPB.2008.35.1.005
The transgenic technologies and their product (living modified organisms) have been developed and commercialized enough to get much attention in terms of their potentials to solve the current global difficulties such as shortage of food and energy. Furthermore, they are expected to make a big role in improving human health levels and creating bio-economy as innovative tools to pursue environmentally sound economic development. However, for the technologies and products to be developed and used in such a way that they continuously give a good impact to human society, first and foremost safety issues surrounding them should be dealt with. Every stage from in-house R&D, pilot field application to on the shelves should be managed to ensure safety following them because many consumers tend to have fear before they get the right or needed information on the modern biotechnology. In this sense, managing research facilities and LMOs for R&D from the point of safety is very crucial in that they are in the early stage of technology or product development. This paper especially deals with those to be complied with by researchers according to the Act on transboundary movement of LMOs, etc, entered into the effect from Jan. 1 2008.
Risk assessment and variety registration of transgenic crops
Lee, Keun-Pyo ; Kim, Dong-Hern ; Kweon, Soon-Jong ; Baek, Hyung-Jin ; Ryu, Tae-Hun ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 13~21
DOI : 10.5010/JPB.2008.35.1.013
Final regulatory steps for commercialization of transgenic crops are risk assessment and variety registration. The risk assessment of transgenic crops requires broad network of scientists, high cost and long term. Developers of transgenic crops, therefore, face to additional challenges to consider theoretical and strategic aspects on risk assessment. The general concept for risk assessment of genetically modified organisms was derived from chemical risk assessment. Due to the complexity of organisms, however, comparative approaches that are substantial equivalence and familarity have been developed. In practical view, the integration of risk assessment is more difficult than the evaluation of each risk factors involving gene flow, toxicity and allergenicity. Variety registration of transgenic crops requires the results of risk assessment compared with non-GM crops and agronomic data analyzed with standard varieties. For economic and fast commercialization, risk assessment process should combined with condition of cultivation test for variety registration.
Current status of development and event-dependent genetic analysis of genetically modified crops in Korea
Jeong, Soon-Chun ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 23~29
DOI : 10.5010/JPB.2008.35.1.023
Development of genetically modified crops using modern biotechnology is regarded as a promising way to combat with ever-increasing human population. Korea attempted to develop its own genetically modified crops essentially for the past 20 years, however no example of commercialization has been announced. Here, I briefly summarized current status of development and risk assessment of genetically modified crops in Korea. Then, I attempted to identify a death valley in the process of commercialization. Based on experience of risk assessment of 15 different genetically modified organisms, I suggested that lack of the screening of elite events may serve as a death valley.
An analysis of research trends on living modified organisms in Korea through questionnaire surveys
Yi, Hoon-Bok ; Choi, Kyung-Hwa ; Chung, Soon-Gee ; Kim, Yong-Ho ; Kim, Hwan-Mook ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 31~39
DOI : 10.5010/JPB.2008.35.1.031
We analyzed the current research trends of living modified organisms (LMO) by questionnaires in the interest of making biosaftey laws and policies in Korea. We executed a pre-survey at the Crop Functional Genomics 2004 conference and obtained LMO research information from 423 LMO research organizations, including 32 national research institutes, 314 universities, and 77 industries. We found that the total 59 kinds of hosts including 26 kinds of plants, 15 kinds of animals, and 18 kinds of microbes were used for LMO research and E. coli was the most common host. The risk of the most experimental hosts was below a biosafety level of 1 (73.8%) and 2 (25.9%). LMO development use purpose was implemented in various developmental uses: 51.3% in test and research use, 19% in health and medical use, and 12.9% in agriculture use. The experiment product, waste product, and products of host for LMO development were 327.2, 223.6, and 13.5 in number of plants; 280.6, 52.4, and 8.7 in number of animals; and
in microbes in 2004. The survey results about how to possess the LMO were very unreliable, because only 10.6% of the researchers returned the questionnaires. Consequently, we strongly suggest the scientific organizations as well as scientists should have more interests in biosafety of LMO research and an LMO biosafety management system should be developed for Korea's future biotechnology.
Expression of laccase in transgenic tobacco chloroplasts
Yoo, Byung-Ho ; Lim, Jong-Min ; Woo, Je-Wook ; Choi, Dong-Woog ; Kim, Sun-Ha ; Choi, Kwan-Sam ; Liu, Jang-Ryol ; Ko, Suk-Min ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 41~45
DOI : 10.5010/JPB.2008.35.1.041
Laccase (EC 18.104.22.168) is a small group of enzymes that catalyze the oxidation of a broad range of phenolic compounds including hazardous and recalcitrant pollutants in the environment. This study attempted to develop an efficient system for production of a recombinant laccase by chloroplast genetic transformation of tobacco. Chloroplast transformation vector was constructed and introduced into the tobacco chloroplast genome using particle bombardment. Chloroplast-transformed plants were subsequently regenerated. PCR and southern blot analyses confirmed stable integration of the laccase gene into the chloroplast genome. Northern blot analysis revealed that mRNA of the laccase gene was highly expressed in chloroplast-transformed plants.
In vitro seed germination and callus formation on flower bud of Korean mistletoe ( Viscum album L. var. cololatum [Kom.] Ohwi)
Kim, Suk-Weon ; Ko, Suk-Min ; Liu, Jang-R. ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 47~53
DOI : 10.5010/JPB.2008.35.1.047
Effects of growth regulators and culture conditions on seed germination, haustorium development, and callus formation of Korean mistletoe (Viscum album var. coloratum (Kom.) Ohwi) were described. Histological examination showed that seed of V. album contained one or two zygotic embryos with rod shape, and actively dividing cells were mainly distributed in radicle region rather than cotyledon of zygotic embryo. The most significant factor for seed germination and haustorium development of V. album was the requirement of the light. Various growth regulators examined in this study failed to substitute the effect of the light on seed germination. The frequency of callus formation was highest at 27.3% when flower buds were cultured onto B5 medium containing
IAA. Explants from other organs were recalcitrant in forming calluses. Culture conditions described in this study could be applied for production of useful metabolites and multiplication of V. album in future.
The characterization of transgenic Chrysanthemum under low temperature condition
Choi, In-Young ; Han, Soo-Gon ; Kang, Chan-Ho ; Song, Young-Ju ; Lee, Wang-Hyu ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 55~61
DOI : 10.5010/JPB.2008.35.1.055
Previous studies on genetic transformation of chrysanthemum using cold regulated gene (BN115) have been conducted and the PCR and Real-Time PCR based method to determine the presence of the transferred cold regulated gene in the chrysanthemum was established. To check whether over-expression of BN115 gene in transgenic chrysanthemum will enhance their tolerance to cold stress, the transgenic chrysanthemum were grown under low temperature condition and several cold signalling including growth characteristics, stoma size and shape, SPAD value and ion leakage test were investigated. The transgenic chrysanthemum in the low temperature growth chamber grow much faster in term of the height, number and size of the leaves than those of wild-type plants and damage of transgenic plant caused by the low temperature was much less than that of wild-type plants. The stoma type and size of transgenic plant leaves grown at
were much similar to of wild-type plant cultured on
It has been found that SPAD value of transgenic plants was much higher than those of wild-type, but the EC density being lower under low temperature condition.
Influences by position of node and existence of leaf on microtuberization in node culture of potato
Hwang, Hye-Yeon ; Lee, Young-Bok ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 63~68
DOI : 10.5010/JPB.2008.35.1.063
Single-node stem pieces ca. 1 cm in length containing a axillary bud were obtained from in vitro plants of potato (Solanum tuberosum L.). The influences by a position of the node and the existence of a leaf at the node were observed in the single-node culture on the 8% sucrose MS medium. The effect of CCC was also investigated for the microtuberization. The apical part node was excellent in the tuberization not to mention shoot length, fresh weight, diameter, the number of node on the in vitro culture of a single-node than the lower part. The differences in the diameter of a tuber formed in the part of the axillary bud on all treatments including the cultivation of the apical part node were not recognized. However, the fresh weight of the tuber showed high value in the tuber formed at the axillary bud of shoot apex part. At 20 days after cultivation, tuberization was promoted in the new stolen that developed from the bud of node with a leaf under SD condition of 8 hours at
. The tuberization from axillary bud of the single-node without leaf was inhibited at high temperature of
regardless of daylength. Whereas, tuberization at
was similar without the difference under SD condition but the tuber formation ratio were low. CCC 500 mg/L promoted tuberization and the effect was also showed even under LD condition at
. The inhibiton of tuberization under LD and high temperature condition could be solved by treatment with CCC.
Development of herbicide tolerant soybean using Agrobacterium tumefaciens
Lee, Ki-Jong ; Park, Hong-Jae ; Yi, Bu-Young ; Lee, Kyeong-Ryeol ; Kim, Myung-Sik ; Woo, Hee-Jong ; Jin, Yong-Moon ; Kweon, Soon-Jong ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 69~74
DOI : 10.5010/JPB.2008.35.1.069
This study aims to establish the efficient soybean transformation system and develop soybean [Glycine max (L.) Merill] transformants using cotyledonary node explants. The cotyledonary node of soybean were co-cultivated with Agrobacterium tumefaciens strains (KYRT1, EHA105). These strains contain the binary vector pCAMBIA3301 which carries a herbicide-resistant far gene. Korean cultivars (Danbaekkong, Eunhakong) and foreign cultivars (Jack, Peking) were the most efficient in regenerating cotyledonary node. Therefore, they were chosen for the transformation. Results showed that the T-DNA transfer reached up to 60% and transformation efficiency reached up to 3% in the cotyledonary node explants from Jack cultivar, co-cultivated with EHA105 strain. Histochemical GUS evaluation showed that 12 individual lines, transformed with the 현 gene, have positive response. The transformed soybeans have been confirmed in the
generation through phenotypic assay using herbicide
and Southern blot analysis.
Introduction of two-step culture method for multiple seed bulb development from shoot tip culture of garlic (Allium sativium L.)
Hwang, Hye-Yeon ; Lee, Young-Bok ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 75~80
DOI : 10.5010/JPB.2008.35.1.075
In vitro culture of shoot tip of garlic (Allium sativium L. cv. Seosan) was carried out to find medium condition of the induction of multiple shoots and bulbing for muliproduction of virus-free seed bulbs. For this work, tank culture was introduced. In shoot tip culture on MS solid medium the induction of multiple shoots and bulbing were better by adding 3% sucrose than 8%. Supplementation with 2mg/L 2ip and 0.2 mg/L IAA in this medium was effective. Three point three shoots including 2.7 bulbs were formed from a shoot tip after cultivation for 30 days on this medium. Bulbing of garlic in liquid culture with plastic water tank of 20L supplied air at the side of the lower part was better by adding 3% sucrose than 8% by subculture for 45 days with shoots obtained from shoot tip culture for 30 days on soid MS medium. Shoot growth was vigorous at 3% sucrose however bulb growth was more effective on the medium of 8% sucrose. Because of the effectiveness on solid medium added 3% sucrose, 2 mg/L 2ip and 0.2 mg/L IAA for initial production of multi-shoot in stem tip culture and the effectiveness in liquid culture with water tank for growth of bulbs, the method of two-step culture could be introduced for the multiple production of seed bulb of high quality. It was more desirable by supply of 0.2 mg/L BA and 0.02 mg/L NAA at tank culture time. But growth of the bulbs became poor by increasing concentration of NAA of the medium.
Plant regeneration from protoplasts-derived from embryogenic callus of Citrus
An, Hyun-Joo ; Lee, Dong-Hoon ; Lee, Ji-Hyun ; Choi, Young-Hun ; Kang, Byoung-Cheorl ; Park, Hyo-Guen ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 81~86
DOI : 10.5010/JPB.2008.35.1.081
This study describes conditions for plant regeneration from protoplasts-derived from embryogenic callus of satsuma mandarin. Plants were generated via somatic embryogenesis. Protoplasts isolated directly from nucellar callus induced from immature ovule of satsuma mandarin cv. Okitsu (Citrus unshiu Marc.) were cultured in 0.6M
medium. Cell division and plating efficiency were affected by protoplast culture method. The liquid over solid method was the most effective for formation of microcalli. Most of microcalli grew rapidly and transferred onto embryoid formation medium. Optimum embryoid formation medium was MT medium containing 1.5 g/L malt extract, 0.146 M sucrose and the medium for plantlet regeneration was MS medium containing 0.09M sucrose, 1.0 mg/L
. No differences were noticed in growth habits and leaf characters such as shape, thickness, and colour between protoplast-derived plants and nucellar seedlings. This plant regeneration system from protoplasts-derived from embryogenic callus provides an alternative way for producing new scion and rootstock cultivar from citrus species which can not be crossed.
Transgenic tobacco culture cells expressing spike protein gene of porcine epidemic diarrhea virus
Yang, Kyoung-Sil ; Kim, Hyeon-Soo ; Kwon, Suk-Yoon ; Kwak, Sang-Soo ; Lee, Haeng-Soon ;
Journal of Plant Biotechnology, volume 35, issue 1, 2008, Pages 87~94
DOI : 10.5010/JPB.2008.35.1.087
Porcine epidemic diarrhea virus (PEDV) is an infectious and highly contagious virus of swine. In order to develop the transgenic tobacco culture cells producing PEDV antigen protein, four vectors expressing PEDV spike protein (SP) gene under the control of a CaMV 35S promoter were constructed. Four fragments of the SP region of PEDV, SP1 (444 bp, 1487-1930 bp), SP2 (1.7 kb, 2300-3987 bp), SP3 (1.4 kb, 1559-2950 bp), and SP4 (2.6 kb, 9-2643 bp) were amplified by PCR and then C-MYC tag was fused to the end of each SP gene, respectively. These cassettes are inserted into the pCAMBIA2300 (named as 35S::SP1-M, 35S::SP2-M 35S::SP3-M, and 35S::SP4-M, respectively). Tobacco (cv. BY-2) cultured cells were transformed by co-cultivation with Agrobacterium tumefaciens harboring expression vector. We selected kanamycin-resistant calli and checked for the presence of the introduced SP gene using PCR, resulting 70% of them showed the foreign gene. We selected the lines with high-level expression of PEDV antigen protein based on dot blot analysis. Southern blot analysis confirmed that the PEDV SP gene was integrated into the genome of the tobacco cultured cells. Northern blot analysis showed that the introduced gene was highly expressed in transgenic cultured cells. Transgenic tobacco cultured cells-derived antigen induced immunogenicity in mice as determined by a plaque reduction neutralization assay. These results suggest that the vectors expressing PEDV spike protein gene in this study will be useful for the development of transgenic plants and cultured cells producing PEDV antigene protein.