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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 35, Issue 4 - Dec 2008
Volume 35, Issue 3 - Sep 2008
Volume 35, Issue 2 - Jun 2008
Volume 35, Issue 1 - Mar 2008
Selecting the target year
Characterization of SID2 that is required for the production of salicylic acid by using β-GLUCURONIDASE and LUCIFERASE reporter system in Arabidoposis
Hong, Mi-Ju ; Cheong, Mi-Sun ; Lee, Ji-Young ; Kim, Hun ; Jeong, Jae-Cheol ; Shen, Mingzhe ; Ali, Zahir ; Park, Bo-Kyung ; Choi, Won-Kyun ; Yun, Dae-Jin ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 169~176
DOI : 10.5010/JPB.2008.35.3.169
Salicylic acid(SA) is a phytohormone that is related to plant defense mechanism. The SA accumulation is triggered by abiotic and biotic stresses. SA acts as a signal molecular compound mediating systemic acquired resistance and hypersensitive response in plant. Although the role of SA has been studied extensively, an understanding of the SA regulatory mechanism is still lacking in plants. In order to comprehend SA regulatory mechanism, we have been transformed with a SID2 promoter:GUS::LUC fusion construct into siz1-2 mutant and wild plant(Col-0). SIZ1 encodes SUMO E3 ligase and negatively regulates SA accumulation in plants. SID2(SALICYLIC ACID INDUCTION DEFICIENT2) is a crucial enzyme of SA biosynthesis. The Arabidopsis SID2 gene encodes isochorismate synthase(ICS) that controls SA level by conversion of chorismate to isochorismate. We compared the regulation of SID2 in wild-type and siz1-2 transgenic plants that express SID2 promoter:GUS::LUC constructs respectively. The expressions of
-GLUCURONIDASE and LUCIFERASE were higher in siz 1-2 transgenic plant without any stress treatment. SID2 promoter:GUS::LUC/siz1-2 transgenic plant will be used as a starting material for isolation of siz1-2 suppressor mutants and genes involved in SA-mediated stress signaling pathway.
Characterization of Oszinc626, knock-out in zinc finger RING-H2 protein gene, in Ac/Ds mutant lines of rice(Oryza sativar L.)
Park, Seul-Ah ; Jung, Yu-Jin ; Ahn, Byung-Ohg ; Yun, Doh-Won ; Ji, Hyeon-So ; Park, Yong-Hwan ; Eun, Moo-Young ; Suh, Seok-Cheol ; Lee, Soon-Youl ; Lee, Myung-Chul ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 177~183
DOI : 10.5010/JPB.2008.35.3.177
Ac/Ds mutant lines of this study were transgenic rice plants, each of which harbored the maize transposable element Ds together with a GUS coding sequence under the control of a promoterless(Ds-GUS). We selected the mutants that were GUS expressed lines, because the GUS positive lines will be useful for identifying gene function in rice. One of these mutants was identified knock-out at Oszinc626(NP_001049991) gene, encoding a RING-H2 zinc-finger protein, by Ds insertion. In this mutant, while primary root development is normal, secondary root development from lateral root was very poor and seed development was incomplete compare with normal plant. RING zinc-finger proteins play important roles in the regulation of development in a variety of organisms. In the plant kingdom, a few genes encoding RING zinc-finger proteins have been documented with visible effects on plant growth and development. The consensus of the RING-H2(C3-H2-C3 type) domain for this group of protein is
. Oszinc626 encodes a predicted protein product of 445 amino acids residues with a molecular mass of 49 kDa, with a RING-zinc-finger motif located at the extreme end of the C-terminus. RT-PCR analysis indicated that the expression of Oszinc626 gene was induced by IAA, cold, dehydration, high-salinity and abscisic acid, but not by 2,4-D, and the transcription of Oszinc626 gene accumulated primarily in rice immature seeds, root meristem and shoots. The gene accumulation patterns were corresponded with GUS expression.
Proteomic analysis of Korean ginseng(Panax ginseng C. A. Meyer) following exposure to salt stress
Kim, Sun-Tae ; Bae, Dong-Won ; Lee, Kyung-Hee ; Hwang, Jung-Eun ; Bang, Kyong-Hwan ; Kim, Young-Chang ; Kim, Ok-Tae ; Yoo, Nam-Hee ; Kang, Kyu-Young ; Hyun, Dong-Yun ; Lim, Chae-Oh ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 185~193
DOI : 10.5010/JPB.2008.35.3.185
We evaluated the response to salt stress of two different ginseng lines, STG3134 and STG3159, which are sensitive and tolerant, respectively, to salt treatment. Plants were exposed to a 5 dS/m salt solution, and chlorophyll fluorescence was measured. STG3134 ginseng was more sensitive than STG3159 to salt stress. To characterize the cellular response to salt stress in the two different lines, changes in protein expression were investigated using a proteomic approach. Total protein was extracted from detached salt-treated leaves of STG3134 and STG3159 ginseng, and then separated by two-dimensional polyacrylamide gel electrophoresis(2-DE). Approximately 468 protein spots were detected by 2-DE and Coommassie brilliant blue staining. Twenty-two proteins were found to be reproducibly up- or down-regulated in response to salt stress. Among these proteins, twelve were identified using MALDI-TOF MS and ESI-Q-TOF and classified into several functional groups: photosynthesis-related proteins(oxygen-evolving enhancer proteins 1 and 2, rubisco and rubisco activase), detoxification proteins(polyphenol oxidase) and defense proteins(
-1,3-glucanase, ribonuclease-like storage protein, and isoflavone reductase-like protein). The protein levels of ribonuclease-like storage protein, which was highly induced in STG3159 ginseng as compared to STG3134, correlated tightly with mRNA transcript levels, as assessed by reverse-transcription(RT)-PCR. Our results indicate that salinity induces changes in the expression levels of specific proteins in the leaves of ginseng plants. These changes may, in turn, playa role in plant adaptation to saline conditions.
Cryopreservation of zygotic embryos of wild yams(Dioscorea spp.) in Korea
Shin, Jong-Hee ; Kang, Dong-Kyoon ; Lim, Jae-Ha ; Sohn, Jae-Keun ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 195~201
DOI : 10.5010/JPB.2008.35.3.195
A simplified technique that cryoprotects zygotic embryos by desiccation was developed for germplasm conservation of wild yam species(Dioscorea spp.) in Korea. Comparative studies with three other cryogenic techniques were conducted. The maximum survival of zygotic embryos were achieved at a frequency of 96.6% when embryos were cryopreserved by the desiccation method. For the successful cryopreservation of yam zygotic embryos, those that were excised from immature/mature seeds were dried in the air stream of a laminar flow cabinet for 30 min at room temperature and then directly immersed in liquid nitrogen.
In vitro micropropagation of Philodendron cannifolium
Han, Bong-Hee ; Park, Byoung-Mo ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 203~208
DOI : 10.5010/JPB.2008.35.3.203
In order to micropropagate uniform plantlets of Philodendron cannifolium in vitro, the shoot tips were cultured on MS media supplemented with
mg/L BA or
mg/L thidiazuron(TDZ). The adventitious multi-bud clusters from basal part of shoots were formed on MS media containing
mg/L BA or
mg/L TDZ. But the shoots grown on MS media with TDZ showed necrosis by the lack of chlorophyll. The adventitious multi-bus clusters were cut into
mm sections and cultured on MS media containing BA and TDZ for shoot proliferation. Shoots were proliferated vigorously on MS medium supplemented with
mg/L BA with up to 30 shoots. But abnormally swollen hard calli were formed from basal parts of shoots on MS media with TDZ and high concentration of BA(10.0 mg/L). The proliferated shoots on same media also showed necrosis by the lack of chlorophyll. The shoot growth and rooting were favorable on MS media containing
mg/L IBA. The rooted plantlets were acclimatizated effectively in soil mixed with perlite 1:vermiculite 1 or vermiculite alone. Fifteen mL of liquid medium containing 10 g/L activated charcoal and 30 g/L sucrose were added in same vessels after small shoots were proliferated to stimulate shoot growth and rooting. After 8 weeks in culture, the shoots were dipped into high concentration of IBA solution. and planted in soil mexed with perlite 1:vermiculite 1. The shoot growth and rooting were favorable in dipping treatments of
ppm IBA solutions for 10 sec.
Shoot regeneration from internode sections of Ardisia pusilla DC.
Lee, Su-Young ; Kim, Young-Soon ; Han, Bong-Hee ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 209~213
DOI : 10.5010/JPB.2008.35.3.209
This study was conducted to regenerate shoots from internode sections(about 1mm in thickness) of Ardicia pusilla de Candolle. Internode sections were cultured on MS medium supplemented with TDZ or both TDZ and IBA. At one month after culture, primodium, which looks like protocorm like body(PLB) of orchid, appeared around swollen internodes. And then it grew and changed into the shape similar to granule of orange at two or three months after culture. At four to five months after culture, explants covered with them became a cluster, and then multiple shoots were regenerated from them. Primodia formation was the best when internode was cultured on MS medium supplemented with 0.25
thidiazuron(TDZ) and 0.5
indole-3-butyric acid(IBA). That internodes were cultured on MS medium supplemented with either higher concentration of TDZ than that of IBA, or equal concentration of TDZ and IBA, or TDZ only was little effective for primodia formation.
Effect of different light sources and ventilation on in vitro shoot growth and rooting of a rare and endangered species, Tsuru-rindo(Tripterospermum japonicum)
Moon, Heung-Kyu ; Park, So-Young ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 215~221
DOI : 10.5010/JPB.2008.35.3.215
Effects of light generated by LEDs on shoot growth and rooting of Tsuru-rindo(Tripterospermum japonicum) were evaluated. Apical shoots(one or two node with 3-4 leaves) were cultured on MS basal medium with 3% sucrose and maintained for four weeks under five different light qualities: fluorescent lamp(F), 100% red LED(R), 70% red LED+30% blue LED(R7B3), 50% red LED+50% blue(R5B5), or 100% blue LED(B). Rooting was promoted by both red light and fluorescent lamp, and the effect was further promoted under the ventilation. Red light enhanced shoot node elongation, whereas blue light appeared to suppress it. Growth of shoots and leaves were enhanced under the ventilation irrespective of the different light qualities. Under the ventilated condition, total fresh weight of plants was highest in R7B3 LED as 257.7 mg per plant. Dry matters, which are used for index of plant growth, were lowest under red light, whereas it was highest under blue light. The dry matter was inclined to getting higher by ascending the ratio of blue light and red light. Total chlorophyll content was highest in both R7B3 LED and R5B5 LED under ventilation as 29.5 and 31.2, respectively. Above results suggest that light quality optimization could be an important factor to foster in vitro growth of the species. Ventilation treatment appeared to be another important factor to induce normal shoot growth and rooting.
In vitro multiple shoot proliferation and plant regeneration in rose(Rosa hybrida L.)
Lee, Su-Young ; Jung, Ji-Hye ; Kim, Jeong-Hee ; Han, Bong-Hee ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 223~228
DOI : 10.5010/JPB.2008.35.3.223
This study was conducted to investigate an optimal condition for shoot proliferation and regenerate shoots from in vitro leaflet and embryogenic calli from in vitro roots in rose. The effect of BAP on shoot proliferation was somewhat different depending upon genotypes or gelling agents. Leaflets with petiole cut from donor shoots which had been cultured in MS medium supplemented with 0.1
NAA for six weeks was effective for regeneration of adventitious buds(ABs) as well as shoot elongation of Rosa hybrida cv. Sweet Pink. Culturing seven leaflet explants per petri plate(
) was effective for regeneration of ABs. Embryogenesis was shown in the calli induced from roots of Rosa hybrida cv. Sweet Pink cultured in the SH medium supplemented with 11
2, 4-D for four weeks. Color of calli induced from roots was yellow although their color was a little different as type of basal medium.
In vitro propagation of a rare and endangered species, Echinosophora koreensis Nakai, by axillary bud culture
Moon, Heung-Kyu ; Kim, Yong-Wook ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 229~234
DOI : 10.5010/JPB.2008.35.3.229
An efficient micropropagation was established by using axillary bud explants from two-year-old tree(Echinosphorea koreensis Nakai), which has been known as a rare and endangered species. Among various basal media tested, DKW medium was shown to be the best for axillary shoot elongation. The addition of both BA and TDZ to the medium induced 6 to 10 shoots per explant during eight weeks of culture, without showing any abnormal morphology at the shoot proliferation stage. However, high concentration of TDZ(>0.05 mg/L) appeared to cause hyperhydration on either leaf or shoot at the later developmental stage. Approximately 20% of shoots produced roots by the addition of 1.0 mg/L NAA but not by IBA(
mg/L). Ex vitro micro-cuttings were better source for root induction; up to 58.6% of the micro-cuttings rooted when 100 mg/L IBA was applied to the soil(vermiculite). More than 90% of plantlets with roots were successfully acclimatized and grew normally in the field. Therefore, we suggest that this endangered tree species can be effectively micropropagated by axillary bud culture system developed in this study.
Effects of a variety of treatments affecting Chinese cabbage protoplast culture, and plant regeneration from protoplast-derived callus
Han, Jeung-Sul ; Yoon, Moo-Kyeong ; Jeong, Mi-Hye ;
Journal of Plant Biotechnology, volume 35, issue 3, 2008, Pages 235~243
DOI : 10.5010/JPB.2008.35.3.235
Here we describe a procedure for Chinese cabbage protoplast culture and effects of various treatments. Chinese cabbage protoplasts were isolated from different parts of young seedlings as using an enzyme mixture, of which yield was maximized in seven hours around after digestion. The highest rate of initial cell division followed by micro-callus formation was obtained in the medium with 1.0 mg/L 2,4-D, 0.5 mg/L NAA, and 1.0 mg/L BA when the cotyledon-derived protoplasts were cultured. Initiation of cell division and micro-callus proliferation significantly depended upon Chinese cabbage genotype under a same culture circumstances. The micro-calli developed from cotyledon tissue of Norang-Bom cultivar successfully grew toward callus colonies on the solidified medium with 0.2 mg/L zeatin and 0.1 mM spermidine. The callus colonies generated de novo shoots at the maximum frequency of 4.3% on the medium with 5.0 mg/L BA and 1.0 mg/L NM. Our results might be helpful for further studies to enhance the regeneration efficiency in Chinese cabbage protoplast culture.