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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 36, Issue 4 - Dec 2009
Volume 36, Issue 3 - Sep 2009
Volume 36, Issue 2 - Jun 2009
Volume 36, Issue 1 - Mar 2009
Selecting the target year
Metabolic engineering for production of industrial oils in transgenic plants
Lee, Kyeong-Ryeol ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 97~105
DOI : 10.5010/JPB.2009.36.2.097
Seed storage lipids of plants, essential for seed germination as energy supplier, have been used for humankind and animal as nutrition sources. Fatty acids of vegetable oils have the characters appropriate for industry based on their chain length, the position and the number of double bonds. So they are used as raw materials for lubricants, cosmetics, soaps, paints and plastics or as energy source such as bio-diesel. However, there is a limit that applies vegetable oils from typical oil crops for industrial uses, mainly because of the mixture of five common fatty acids. Therefore, identification of unusual fatty acids for industrial uses from diverse plant resources and metabolic engineering to produce unusual fatty acids have been carried out in Arabidopsis as a model for the study of oilseed biology. Here, we discuss the unusual fatty acids for industrial uses, the genes synthesizing them in lipid metabolism, and the current limits in production of transgenic plants accumulating unusual fatty acid in their seeds. In addition, we describe our work on metabolic engineering of Brassica napus for the production of the unusual fatty acid ricinoleic acid in the seed, because of its industrial uses.
A survey of the genetic components introduced into approved GM crops
Woo, Hee-Jong ; Chung, Chan-Mi ; Shin, Kong-Sik ; Ji, Hyeon-So ; Lee, Ki-Jong ; Suh, Seok-Chul ; Kweon, Soon-Jong ; Cho, Yong-Gu ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 106~114
DOI : 10.5010/JPB.2009.36.2.106
Genetic components introduced into approved GM crops are a key subject for safety assessment and provide a basis for the development of detection methods for GM crops. In order to understand the genetic components in approved GM crops comprehensively, we screened the genetic vector maps of GM crops that had been approved for commercialization around the world. A total of 64 varieties from 5 major GM crop species (maize, canola, cotton, soybean, and tomato) were subjected to analysis. The genetic components included genes, promoters, terminators, and selection marker. This survey may be useful for researchers who develop GM crops and methods for detecting GM crops.
Molecular biological characteristics and analysis using the specific markers of leaf folder-resistant GM rice
Shin, Kong-Sik ; Lee, Si-Myoung ; Lim, Sun-Hyung ; Woo, Hee-Jong ; Cho, Hyun-Suk ; Lee, Kyeong-Ryeol ; Lee, Myung-Chul ; Kweon, Soon-Jong ; Suh, Seok-Cheol ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 115~123
DOI : 10.5010/JPB.2009.36.2.115
In recent years, several genetically modified (GM) crops have been developed worldwide through the recombinant DNA technology and commercialized by various agricultural biotechnological companies. Commercialization of GM crops will be required the assesment of risks associated with the release of GM crops. In advance of the commercial release of GM crops, developer should submit the several information on GM crops for approval. In this study, we carried out to provide the molecular data for the risk assessment of GM rice containing insect-resistant gene, modified Cry1Ac (CryIAc1). Through the molecular analysis with CryIAc1 induced GM rice, we confirmed the steady integration and expression of transgene, the transgene copy number, the adjacent region sequences of inserted gene into rice genome, and the transgene stability in progenies. For the qualitative PCR detection methods, specific primer pairs were designed on the basis of integration sequences, and construct- and event-specific detection markers were developed for leaf folder-resistant rice, Cr7-1 line. From these results, we demonstrated that the molecular data and the PCR detection methods of leaf folderresistant GM rice could be acceptable to conduct the biosafety and environment risk assessment.
Morphological and genetic differences among white-, red- and blue colored root lines in Codonopsis lanceolata
Kim, Ji-Ah ; Bae, Kee-Hwa ; Kwon, Hye-Kyoung ; Yi, Jae-Seon ; Choi, Yong-Eui ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 124~129
DOI : 10.5010/JPB.2009.36.2.124
In general, the root color of Codonopsis lanceolata is white, but red or blue-colored root is found at a low frequency in nature. Red or blue-colored roots have scarcity value, thus farmers wish to produce colored roots. The factors for determining the color of roots are unclear whether the color is controlled by genetically or simply by environmentally such as soil environment. Using in vitro culture system which is advantageous for setting of the same culture condition, we analyzed the physiological and morphological characteristics and genetic differences among red-, blue- and white lines of C. lanceolata. In the red colored roots, stems of in vitro cultured plantlet were colored in dark red pigment. Histological analysis revealed that the red pigment was accumulated in the outer cortex layer of the stem and determined as anthocyanin. Chlorophyll contents in red root lines were higher than those in white- and blue root lines. Plantlets from red roots were smaller in both shoot length and total leaf area than those from white- and blue roots. Genetic differences among the three different colored C. lanceolata were determined by RAPD (Randomly Amplified Polymorphic DNA) analysis. Each line of colored roots had clear DNA polymorphism. These results indicate that the occurrence of red- and blue colored roots in nature was determined by genetic factors rather than soil enviromental conditions.
Structural analysis of expressed sequence tags inimmature seed of Oryza sativa L.
Yoon, Ung-Han ; Lee, Gang-Seob ; Lee, Jung-Sook ; Hahn, Jang-Ho ; Kim, Chang-Kug ; Kikuch, Shoshi ; Satoh, Kouji ; Kim, Jin-A ; Lee, Jeong-Hwa ; Lee, Tae-Ho ; Kim, Yong-Hwan ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 130~136
DOI : 10.5010/JPB.2009.36.2.130
Rice (Oryza sativa) is the most important staple crop in Korea. With its small genome size of 389Mb, rice is a model plant for genome research. We analyzed expressed sequence tag (EST) clones from immature seeds of rice (cv. Ilpum) at 20 days after heading. The 25,668 EST clones were clustered by using SeqMan program and 7,509 clones were selected as unique clones. We compared the 7,509 unique genes with KOME database including the 32,127 FL-cDNA in rice. Finally, 4,990 clones were homologous and 2,519 clones non-homologous to FL-cDNA clones. In addition, we mapped the 7,509 cDNA clones by using TIGR rice pseudomolecule version 5. Ultimately, 7,347 clones were matched to be significant clones related to the TIGR rice pseudomolecules, but 162 clones were unmapped. For the clustering of orthologous group genes, we further analyzed the 7,509 EST clones from immature seeds using NCBI clusters of orthologous groups database. Among the clones, 4,968 clones were categorized into information storage and processing, cellular processes and signaling, metabolism and poorly characterized genes, proportioning 799 (14.89%), 1,536 (28.3%), 1,148 (21.2%) and 1,936 (35.7%) clones to the previous four categories, respectively.
Factors for high frequency plant regeneration in tissue cultures of Indian mustard (Brassica juncea L.)
Bhuiyan, Mohammed Shafi Ullah ; Min, Sung-Ran ; Choi, Kwan-Sam ; Lim, Yong-Pyo ; Liu, Jang-Ryol ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 137~143
DOI : 10.5010/JPB.2009.36.2.137
An efficient system for high frequency plant regeneration was established through investigating various factors such as plant growth regulator combinations, explant types and ages, and addition of
influenced on shoot regeneration in Brassica juncea L. cv. BARI sarisha-10. Murashige and Skoog (MS) medium supplemented with 0.1 mg/L NAA (1-naphthaleneacetic acid) and 1 mg/L BA (6-benzyladenine) showed the maximum shoot regeneration frequency (56.67%) among the different combinations of NAA and BA. Explant type, explant age, and addition of
also significantly affected shoot regeneration. Of the four type of explants (cotyledon, hypocotyl, root, and leaf explants)- cotyledon explants produced the highest shoot regeneration frequency and hypocotyls explants produced the highest number of shoots per explant, whereas root explants did not produce any shoot. The cotyledonary explants from Four-day-old seedlings showed the maximum shoot regeneration frequency and number of shoots per explant. Shoot regeneration frequency increased significantly by adding
to the medium. Two mg/L
appeared to be the best for shoot regeneration with the highest shoot regeneration frequency (86.67%) and number of shoots per explant (7.5 shoots). Considerable variation in shoot regeneration from cotyledonay explants was observed within the B. juncea L. genotypes. The shoot regeneration frequency ranged from 47.78% for cv. Shambol to 91.11% for cv. Rai-5. In terms of the number of shoots produced per explant, B. juncea L. cv. Daulot showed the maximum efficiency. MS medium supplemented with 0.1 mg/L NAA showed the highest frequency of rooting. The regenerated plantlets were transferred to pot soil and grown to maturity in the greenhouse. All plants were fertile and morphologically identical with the source plants.
Transfer of SOD2 or NDP kinase 2 genes into purebred lines of petunia
Lee, Su-Young ; Han, Bong-Hee ; Noh, Eun-Woon ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 144~148
DOI : 10.5010/JPB.2009.36.2.144
The transfer of Mn-Superoxide Dismutase (SOD2) gene, complex gene (SA) of CuZnSOD and ascorbate peroxidase (APX), and NDP kinase 2 (NDPK2) gene into Korean 4 cultivars (cvs. Millenium White, Glory Blue, Glory Red, and Glory Purple) and 15 purebred lines of petunia was conducted using Agrobaterium-mediated technique. Two (Wongyo A2-16 and A2-36) of 15 purebred lines and one (cv. Glory Red) of 4 cultivars were effective for the transfer of SOD2 gene. The putative transgenic plants survived on the 2nd selection medium were 124. From PCR analysis, 118 (derived from 4 cultivars and 2 purebred lines) of 124 plants were confirmed to contain marker (npt II ) gene, while 58 of 118 plants did not have target genes. There were no plants with both npt II and SA genes. Twenty seven of 28 SOD2 transgenic plants were re-confirmed as transformants by Sothern analysis. SOD2 and NDPK2 genes were expressed in the transgenic petunias as the ratio of 77.8 to 100.0 % and 23.5%, respectively. T1 seeds were obtained from 36 acclimated transgenic plants (SOD2 34 plus NDPK2) in a glasshouse by self-pollination.
Effects of polyamines on hydrogen peroxide-scavenging enzymes in radish seedling plants under paraquat stress
Jin, Chang-Duck ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 149~156
DOI : 10.5010/JPB.2009.36.2.149
Application of exogenous polyamines (PAs) reduced the paraquat (PQ)-induced cotyledon injuries in radish seedling plants with 1 mM spermidine (Spd) being the most effective protectant. PQ injury symptoms in the cotyledons, e.g., large accumulation of
, and losses of fresh weight, chlorophyll, and proteins, were significantly alleviated. Likewise, analysis of
-scavenging enzymes such as catalase (CAT) and guaiacol peroxidase (GPX) showed that pretreatment with Spd among PAs remarkably increased total CAT activity and strongly retarded PQ-induced rapid decline in total GPX activity. In a native gel assay, one CAT isozyme (CAT1) and two GPX isozymes (GPX1 and a newly synthesized GPX isozyme) proved to be more responsible for PQ tolerance, as manifested by the strong increases in their activities by Spd pretreatment. Based on these results, we can suggest that PAs (especially 1 mM Spd) may function as antioxidant protectors by invoking CAT and GPX enzymes which control the endogenous
level in radish cotyledons exposed to PQ.
High frequency plant proliferation via direct fronds regeneration of Korean endemic duckweed species
Oh, Myung-Jin ; Park, Jong-Mi ; Ko, Suk-Min ; Liu, Jang R. ; Kim, Suk-Weon ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 157~162
DOI : 10.5010/JPB.2009.36.2.157
High frequency plant proliferation system via direct frond regeneration of endemic duckweed plants Lemna paucicostata and Spirodela polyrhiza was established. Fronds of L. paucicostata and S. polyrhiza were able to multiply half-strength MS basal medium without plant growth regulators. However, addition of BA at a range of 1 to 3 mg/L was more effective than high concentration of BA treatments for fronds proliferation. Also half-strength MS salts was suitable for the fronds proliferation. Increase of salts concentration had inhibitory effect on fronds proliferation. Also the frequency of callus formation from fronds of L. paucicostata was 3.3%, when they cultured onto 1/2 MS medium supplemented with 1 mg/L of BA. Similarly the frequency of callus formation from S. polyrhiza was very low. After subculture of white globular structures derived from fronds of L. paucicostata, numerous globular somatic embryos and calluses were developed onto the surface of fronds. However these somatic embryos did not fully develop into normal plants when transferred to 1/2 MS basal medium. Therefore direct frond regeneration system was more efficient for mass proliferation of L. paucicostata and S. polyrhiza. The plant regeneration system of L. paucicostata and S. polyrhiza established in this study, might be applied to mass proliferation and genetic transformation for molecular breeding.
Induction and in vitro proliferation of adventitious roots in Dendropanax morbifera
Bae, Kee-Hwa ; Kim, Ji-Ah ; Choi, Yong-Eui ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 163~169
DOI : 10.5010/JPB.2009.36.2.163
Dendropanax morbifera (Araliaceae) is an endemic species in Korea and distributed in the southern part of Korea. The roots and stems of this plant have been used for folk medicine for the treatment of migraine headache, dysmenorrheal, and remove wind dampness and for Vanishes production. Production of adventitious roots in D. morbifera by in vitro cultures could be used as alternatives materials. Leaf, stem, and root segments from D. morbifera seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the leaf segment. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximum induction of adventitious root was obtained on medium with 1.0 mg/L IBA. In liquid culture, growth of root was best 1/2MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. This study demonstrated for the first time to produce adventitious roots in D. morbifera.
Effect of cytokinin on adventitious shoot formation and plant regeneration from explants of Pulsatilla koreana NAKAI
Liam, Yu-Ji ; Iin, Guan-Zhe ; Kim, Won-Bae ; Yoo, Dong-Lim ; Zhao, Xiao-Mei ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 170~173
DOI : 10.5010/JPB.2009.36.2.170
Leaf and petiole explants of Pulsatilla koreana NAKAI were cultured on MS medium supplemented with various concentrations of zeatin, kinetin or BAP combined with 0.05 mg/L IAA. After 6 weeks of culture, effects of cytokinin on adventitious shoot formation from explants were investigated. The highest frequency of shoot formation was obtained when petiole explants were cultured on medium with 0.5 mg/L zeatin and 0.05 mg/L IAA. Regenerated shoot were transferred on to root induction medium. The best root formation was observed at 1/2 MS medium with 1.5 mg/L NAA. Rooted plantlets were transplanted to a mixture of perlit and soil (1:3), where they were successfully acclimatized.
In vitro plant regeneration from axillary buds of Hibiscus syriacus L.
Jeon, Seo-Bum ; Kang, Seung-Won ; Kim, Wan-Soon ; Lee, Gung-Pyo ; Kim, Sun-Hyung ; Seo, Sang-Gyu ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 174~178
DOI : 10.5010/JPB.2009.36.2.174
Presently, we report a simple, reproducible and high frequency plant regeneration in Hibiscus syriacus L. using axillary buds. H. syriacus was regenerated from axillary buds directly or through a callus phase. Regenerated shoots were directly induced from young and fresh axillary buds cultured on Murashige and Skoog medium (MS) supplemented with 0.01 mg/L of the growth regulator thidiazuron (TDZ) after 2 weeks of culture. Directly induced shoots were transferred to hormone-free MS medium and root development was observed after 6 weeks. On the other hand, old and stale axillary buds were regenerated to shoots via callus induction on MS medium containing 0.01–2 mg/L TDZ after 4 weeks. A TDZ concentration of 0.01 mg/L was most effective in callus formation. Green callus was transferred to MS medium containing 0.01 mg/L α-naphthalene acetic acid (NAA) and 0.5 mg/L benzylaminopurine (BA). After 4 weeks, callus had developed into multiple shoots. Plantlets were formed from 10 week cultures of single shoots on hormone-free MS medium. Regenerated plantlets were cultured on MS medium for one month and then transferred to pots containing garden soil. Potted plants were acclimatized for one month and grown to maturity under greenhouse conditions. The present study has shown that various concentrations of plant growth regulator can be effective for in vitro plant regeneration of H. syriacus. The direct and indirect regeneration protocol presented here will be useful for understanding the manipulation and propagation of H. syriacus.
Effect of reactive oxygen species on floral senescence in Hibiscus syriacus L.
Seo, Sang-Kyu ; Kim, Sun-Hyung ; Lee, Gung-Pyo ; Kang, Seung-Won ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 179~183
DOI : 10.5010/JPB.2009.36.2.179
To understand the effect of reactive oxygen species (ROS) on floral senescence in Hibiscus syriacus L., we have investigated change in relative water potential, malondialdehyde (MDA) content,
content and the activity of antioxidative enzymes in the petals during flower opening and senescence. Hibiscus flowers were achieved full bloom at early morning and started to in-rolling and showed petal in-rolling over than 50% at 24 h and 36 h after full bloom, respectively. The flower was a decrease in fresh weight by 30% and showed water loss with floral senescence. MDA content and activity of antioxidative enzymes such as APX, GR and CAT were showed no significant change until 36 h after full bloom. In the flower 48 h after full bloom that showed complete petal in-rolling and wilting, however, activity of antioxidative enzymes and
content was greatly increased as compared with 0 h after full bloom. These results suggest that reactive oxygen species are related to accelerating the later senescence more than inducing the early senescence during Hibiscus flower senescence.
Influence of pretreatment medium, fresh medium addition, and culture plate size on the production of embryos in isolated microspore culture of hot pepper (Capsicum annuum L.)
Park, Eun-Joon ; Kim, Jin-Ae ; Kim, Moon-Za ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 184~192
DOI : 10.5010/JPB.2009.36.2.184
The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment (
), high frequency embryo production was obtained when the sucrosestarvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from
cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production.
Establishment of high frequency plant regeneration system from leaf explants of Pinellia koreana via bulblets formation
Oh, Myung-Jin ; Park, Jong-Mi ; Lee, Bu-Youn ; Choi, Pil-Son ; Tae, Kyoung-Hwan ; Kim, Suk-Weon ;
Journal of Plant Biotechnology, volume 36, issue 2, 2009, Pages 193~196
DOI : 10.5010/JPB.2009.36.2.193
Pinellia koreana K-H Tae & J-H Kim is a recently discovered Korea endemic medicinal plant species whose natural habitat is rapidly destroyed by industrial development. Described in this paper are culture conditions for high frequency plant regeneration via bulblet formation from leaf explant cultures of P. koreana. Leaf explants formed white nodular structures and off-white calluses at a frequency of 91.2% when cultured on MS medium supplemented with 2 mg/L BA and 0.5 mg/L NAA. However, the frequency of white nodular structures and off-white calluses formation was slightly decreased with an increasing concentration of NAA up to 4 mg/L, where the frequency reached 31.7%. Most petiole explants did not form white nodular structures and off-white calluses except the combination treatment of 2 mg/L BA and 2 mg/L NAA. Upon transfer onto MS basal medium, over 90% of nodular structures gave rise to numerous bulblets and developed into plantlets. Plantlets regenerated from bulblets were transplanted to potting soil and grown to maturity at a survival rate of over 95% in a growth chamber. Therefore, the in vitro plant regeneration system of P. koreana obtained in this study will be useful for mass propagation and long-term preservation of genetic resources of P. koreana.