Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 36, Issue 4 - Dec 2009
Volume 36, Issue 3 - Sep 2009
Volume 36, Issue 2 - Jun 2009
Volume 36, Issue 1 - Mar 2009
Selecting the target year
Strategies of development of environmentally friendly industrial sweetpotato on marginal lands by molecular breeding
Kim, Myoung-Duck ; Ahn, Young-Ock ; Kim, Yun-Hee ; Kim, Cha-Young ; Lee, Jeung-Joo ; Jeong, Jae-Cheol ; Lee, Haeng-Soon ; Mok, Il-Gin ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 197~201
DOI : 10.5010/JPB.2009.36.3.197
The food self-support rate on the basis of cereals in Korea is approximately 27%, which will threaten the national food security. The dramatic increase in population accompanied by rapid industrialization in developing countries has caused imbalances in the supply of food and energy. To cope with these global crises over food and energy supplies as well as environmental problems, it is urgently required to develop new environmentally friendly industrial crop varieties to be grown on marginal lands including desertification areas for sustainable development. Sweetpotato (Ipomoea batatas (L.) Lam.) ranks seventh in annual production among food crops in the world. Its wide adaptability on marginal lands and rich nutritional content provide a high potential for preventing malnutrition and enhancing food security in the developing countries. In addition, sweetpotato can be developed as a bioreactor to produce valuable industrial materials including bio-ethanol, functional feed and antioxidants by molecular breeding. In this respect, we focus on the molecular breeding of sweetpotato with multi-function on marginal lands. The strategies for development of environmentally friendly industrial sweetpotato will be introduced and discussed.
Genetic resources of sweetpotato for industrial use
Mok, Il-Gin ; Zhao, Donglan ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 202~206
DOI : 10.5010/JPB.2009.36.3.202
In many countries including China and U.S., researchers are developing methods to use sweetpotato as raw material for biofuel. We consider the sweetpotato is not only a source of green fuel, it eventually will provide various material including paper, adhesives, biodegradable plastics, and secondary metabolites. Sweetpotato is one of the high efficiency crop because it yields more calories per unit area than either maize or potato, and it requires the shortest growing cycle of the root crops grown in the tropics. Sweetpotato is the most useful crop for the coming starchbased industry era. Sweetpotato genetic resources are collected, characterized, evaluated, and maintained by U.S., China, Japan, and the International Potato Center. New varieties of sweetpotato using the proper genetic resources and molecular breeding will be developed to cope with the global food and energy in 21st century.
Current status on metabolic engineering of starch in sweetpotato
Ahn, Young-Ock ; Yang, Kyoung-Sil ; Kim, Sun-Hyung ; Kwak, Sang-Soo ; Lee, Haeng-Soon ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 207~213
DOI : 10.5010/JPB.2009.36.3.207
Starch serves not only as an energy source for plants, animals, and humans but also as an environmentally friendly alternative for fossil fuels. Progress in understanding of starch biosynthesis, and the isolation of many genes involved in this process have enabled the genetic modification of crops in a rational manner to produce novel starches with improved functionality. Starch is composed of two glucose polymers, amylose and amylopectin. The amylose and amylopectin ratio in starch affects its physical and physicochemical properties. Alteration in starch structure can be achieved by modifying genes encoding the enzymes responsible for starch biosynthesis and starch hydrolysis. Here, we describe recent findings concerning the starch modification in sweetpotato. Sweetpotato [Ipomoea batatas (L.) Lam] ranks seventh in annual production among food crops in the world as an important starch source. To develop transgenic sweetpotato plants with modifying starch composition, we constructed transformation vectors overexpressing granule bound starch synthase I and inhibiting amylopectin synthesis genes such as starch branching enzyme and isoamylase under the control of 35S promoter, respectively. Transformation of sweetpotato (cv. Yulmi) is in progress.
Contents of low molecular weight antioxidants in the leaves of different sweetpotato cultivars at harvest
Ahn, Young-Ock ; Kim, Sun-Ha ; Lee, Haeng-Soon ; Lee, Joon-Seol ; Ma, Daifu ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 214~218
DOI : 10.5010/JPB.2009.36.3.214
Sweetpotato [Ipomoea batatas (L.) Lam] leaves are excellent source of low molecular weight antioxidants such as polyphenols, anthocyanins and carotenoids compared to other leafy vegetables. Endogenous antioxidants in sweetpotato help our bodies to prevent ageing, heart diseases and cancer. In this study, to develop the proper cultivars for the functional feed materials, we investigated the contents of anthocyanin,
-carotene, and polyphenols as well as DPPH radical scavenging activity in leaves of 14 different cultivars at the time of the harvest. They showed a diverse antioxidation activity. In DPPH radical scavenging activity, cultivars of Nanjing 9, Yulmi and Shinzami showed higher activity, whereas cv. Huiza 6 showed the lowest. Cultivars of Shinzami and Shinhwangmi had the highest anthocyanin (3.5 mg/g fr wt) and polyphenol (15.8 mg/g fr wt) content, respectively. Interestingly, there was a high correlation between cultivars with colorful pigments in storage roots and antioxidants activity in leaves. These results suggest that sweetpotato leaves with high antioxidant activity at harvest would be suitable for functional feed materials.
Selection of oxidative stress-tolerant sweetpotato cultivars for cultivation on marginal lands
Kim, Yun-Hee ; Park, Sung-Chul ; Yang, Kyoung-Sil ; Zhou, Zhilin ; Zhao, Donglan ; Ma, Daifu ; Jeong, Jae-Cheol ; Lee, Haeng-Soon ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 219~223
DOI : 10.5010/JPB.2009.36.3.219
Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress.
Development of transgenic sweet potato producing human lactoferrin
Min, Sung-Ran ; Kim, Jae-Wha ; Jeong, Won-Joong ; Lee, Young-Bok ; Liu, Jang R. ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 224~229
DOI : 10.5010/JPB.2009.36.3.224
Human lactoferrin is an iron-binding glycoprotein with many biological activities, including the protection against microbial and virus infection and stimulation of the immune system. We introduced a human lactoferrin (hLf) cDNA under the control of 35S promoter into sweet potato by particle bombardment. Transgenic plants were regenerated via somatic embryogenesis. Transgenic plants were produced typical tuberous roots in soil. PCR, Southern and northern analyses confirmed that the hLf cDNA was incorporated into the plant genome and was properly expressed in plants. Western blot analysis showed that the 80 kDa full length hLf protein was produced in transgenic tuberous roots. Overall results indicated that sweet potato would be an excellent host to produce human therapeutic proteins.
Effect of TIBA, PCIB and phloroglucinol on somatic embryo maturation and germination in Japanese larch (Larix leptolepis)
Kim, Yong-Wook ; Moon, Heung-Kyu ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 230~235
DOI : 10.5010/JPB.2009.36.3.230
The effect of auxin transport inhibitor (TIBA and PCIB) or auxin synergist (phloroglucinol) on somatic embryo maturation and germination in Japanese larch (Larix leptolepis) was examined. The addition of 15.8 mg/L ABA+5.0 mg/L PCIB showed most promoted the maturation of cotyledon -staged somatic embryos (177.7/90 mg ESM). In contrast, with treatment of 5.0 mg/L PCIB or 5.0 mg/L TIBA, no somatic embryos were obtained. Considering from this result, PCIB or TIBA alone could not substitute for exogenously supplied ABA for maturation of somatic embryos. In the test of below concentration of 5.0 mg/L PCIB, the highest results were recorded in 15.8 mg/L ABA+2.0 mg/L PCIB (109.3/90 mg ESM) or 15.8 mg/L ABA+5.0 mg/L PCIB (103.7/90 mg ESM). However, 5.0 mg/L phloroglucinol (0/90 mg ESM) or no ABA addition (3/90 mg ESM) had little influence on somatic embryos maturation. In germination study, the highest frequency of plantlet regeneration obtained from the somatic embryos which had matured on 15.8 mg/L ABA+5.0 mg/L PCIB (67.9%). However, either 5.0 mg/L PCIB nor 5.0 mg/L TIBA resulted in obtained from plantlets.
Somatic embryogenesis and in vitro plant regeneration from various explants of the halophyte Leymus chinensis (Trin.)
Sun, Yan Lin ; Hong, Soon-Kwan ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 236~243
DOI : 10.5010/JPB.2009.36.3.236
The halophyte Leymus chinensis (Trin.) is a perennial rhizome grass (tribe Gramineae) that is widely distributed throughout China, Mongolia and Siberia. This study was conducted to investigate an optimal condition for plant regeneration from mature seeds, leaf base segments, and root segments in L. chinensis. Plant growth regulators affecting embryogenic callus induction and plant regeneration were investigated by four-factor-three-level [L9 (34)] orthogonal test in this study. The effects of explants types (mature seeds, leaf base segments and root segments), callus types, medium types were examined in this study. Wild type (WT) and Jisheng No. 1 plants (JS) were used for primary callus induction. A clear explants difference was seen during callus induction; mature seeds were considered as the preferred explants; and the highest frequency of callus induction was obtained in Medium 6 using mature seeds as explants in WT. Plant regeneration ability was evaluated by frequencies of green callus forming, shooting, rooting, and shooting with roots. Effect of α-naphthalene acetic acid (NAA) on shoot regeneration was remarkable with the highest frequency of 70.8% in WT after 2-month culture. The medium with 0.2- 0.5 mg/L NAA was found to have the highest shoot induction. All regenerated shoots were successfully rooted when transferred on half-strength Murashige and Skoog (MS) basal medium. The acclimatized plantlets were grown to mature with flowering and seeds setting in green house conditions.
Different oxidative burst patterns occur during host and nonhost resistance responses triggered by Xanthomonas campestris in pepper
Kwak, Youn-Sig ; Han, Ki-Soo ; Lee, Jung-Han ; Lee, Kyung-Hee ; Chung, Woo-Sik ; Mysore, Kirankumar S. ; Kwon, Young-Sang ; Kim, Hee-Kyu ; Bae, Dong-Won ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 244~254
DOI : 10.5010/JPB.2009.36.3.244
The hypersensitive reaction (HR) is the most common plant defense reaction against pathogens. HR is produced during both host- and nonhost-incompatible interactions. Several reports suggest that similarities exist between host and nonhost resistances. We assayed the pattern of generation of reactive oxygen species (ROS) and scavenging enzyme activities during nonhost pathogen-plant interactions (Xanthomonas campestris pv. campestris/Capsicum annuum L.) and incompatible host pathogen-plant interactions (Xanthomonas campestris pv. vesicatoria race1/Capsicum annuum L.). Both
accumulated much faster during nonhost resistance when compared to the host resistance. The scavenging enzyme activities of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) were also different during the host- and nonhost-incompatible interactions. CAT activity was much higher during nonhost resistance, and several new isozymes of SOD and POX were detected during nonhost resistance when compared to the host resistance. Lipoxygenase (LOX) activity was higher in host resistance than nonhost resistance during the early stages of infection. Interestingly, the nitric oxide (NO) radical accumulated equal amounts during both host and nonhost resistance at early stages of infection. Further studies are needed to determine the specific pathways underlying these differences between host and nonhost resistance responses.
Differential expression of soybean SLTI100 gene encoding translation elongation factor 1A by abiotic stresses
Chung, Eun-Sook ; Cho, Chang-Woo ; So, Hyun-A ; Yun, Bo-Hyun ; Lee, Jai-Heon ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 255~260
DOI : 10.5010/JPB.2009.36.3.255
The translation elongation factor 1A, eEF1A, catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome by a GTP-dependent mechanism. By subtractive suppression hybridization technique, we have isolated a soybean low-temperature inducible gene, SLTI100 encoding translation elongation factor 1A. Multiple sequence alignments and phylogenic analysis showed that SLTI100 and other eEF1As originated from diverse organisms are highly conserved. RNA expression of SLTI100 was specifically induced by low temperature, high salt, ABA, or drought stress. Based on the subcellular localization of the corresponding gene product fused to GFP, we were able to confirm that SLTI100-GFP was restricted to the nucleus and cytoplasm. We propose that soybean eEF1A may play an important role in translational regulation during abiotic stress responses in plants.
Debates on the isolation distances to segregate fields with GM crops from fields with non-GM crops for the establishment of their coexistence
Lee, Shin-Woo ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 261~267
DOI : 10.5010/JPB.2009.36.3.261
The coexistence policy of GM and non-GM crops is still on the debates in EU since “the recommendation on guidelines for the development of national strategies and best practices to ensure the coexistence of GM crops with conventional and organic farming” has been reported in 2003. The major issues are maximum tolerance level of GMO admixture and minimum isolation distances of GM fields with others including conventional, organic and seeds production. Majority of member states in EU proposed that the tolerance level of GMO admixture must be more strictly controlled, in particular in the fields for organic crops and seeds production. To this end, it was proposed that minimum isolation distances to segregate GM crops from fields with organic crops and seeds production need to be further extended than those of conventional crops since cross pollination with other crops adjacent GM fields is known as the most prevalent source for GMO contamination. In these circumstances, it is strongly suggested that the current legislations need to be revised including the minimum isolation distances of fields for each species before field cultivation for a commercial GM crop is approved for the first time in South Korea.
Cholera Toxin B Subunit-Porphyromonas gingivalis Fimbrial Antigen Fusion Protein Production in Transgenic Potato
Lee, Jin-Yong ; Kim, Mi-Young ; Jeong, Dong-Keun ; Yang, Moon-Sik ; Kim, Tae-Geum ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 268~274
DOI : 10.5010/JPB.2009.36.3.268
Porphyromonas gingivalis, the gram-negative anaerobic oral bacterium, initiates periodontal disease by binding to saliva-coated oral surface. The cholera toxin B subunit (CTB) genetically linked to FimA1 (1-200 aa) or FimA2 (201-337 aa) of the P. gingivalis fimbrial antigen were introduced into Solanum tuberosum cells by Agrobacterium tumefaciens-mediated transformation method. The integration of CTB-FimA1 or CTB-FimA2 fusion genes were confirmed in the chromosome of transformed leaves by genomic DNA PCR amplification method. Synthesis and assembly of the CTB-FimA fusion proteins into oligomeric structures with pentamer size was detected in transformed tuber extracts by immunoblot analysis. The binding activities of CTB-FimA fusion proteins to intestinal epithelial cell membrane receptors were confirmed by GM1-ganglioside enzyme-linked immunosorbent assay (GM1-ELISA). The ELISA showed that the expression levels of the CTB-FimA1 or CTB-FimA2 fusion proteins were 0.0019, 0.002% of the total soluble protein in transgenic tuber tissues, respectively The synthesis of CTB-FimA monomers and their assembly into biologically active oligomers in transformed potato tuber tissues demonstrates the feasibility of using edible plants for the production of enterocyte targeted fimbrial antigens that could elicit mucosal immune responses.
Comparison of chrysanthemum cultivars based on direct shoot regeneration rates in tissue culture
Han, Bong-Hee ; Lee, Su-Young ; Park, Byoung-Mo ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 275~280
DOI : 10.5010/JPB.2009.36.3.275
Direct shoot regeneration from leaf or internode or petiole segments was conducted in 33 cultivars of chrysanthemum. Shoot regeneration rates varied according to cultivars, culture media, and explant types. The high shoot regeneration rate of more than 70% in 15 cultivars (‘Pink Pangpang’, ‘Orange Memory’, ‘Relance’, ‘Zinba’, ‘Beakma’, ‘Innocence’, ‘Sunny Pangpang’, ‘Euro Yellow’, ‘Dublin’, ‘Boramae’, ‘Peak’, ‘Euro White’, ‘Vesuvio White’, ‘Linneker Salmon’ and ‘Pink Pride’) and 2 ones (‘Forward’ and ‘Agason’) was obtained from the segments of leaves and internodes, respectively, cultured on MS medium containing 1.0 mg-
BAP, 0.5 mg-
IAA and 30 g-
sucrose. That in 6 cultivars (‘Shuhonochikara’, ‘Hakunosen’, ‘Whitney Pangpang’, ‘Plaisir D’Amour’, ‘Grace’ and ‘Kumsu’) was observed from the segments of leaves or internodes cultured on 1/2 MS medium 1.0 mg-
BAP, 0.5 mg-
IAA and 15 g-
sucrose In case of 3 cultivars (‘Ilweol’, ‘Puma White’ and ‘Sharon’), when internode explants excised from mother plants, which were pre-cultured on MS medium containing 2 g-
activated charcoal and 30 g-
sucrose for two months in the dark, and cultured on MS medium containing 1.0 mg-
BAP, 0.5 mg-
IAA and 30 g-
sucrose, that was shown. Seven cultivars including ‘Puma Yellow’, ‘Argus’, ‘Yes Morning’, ‘Whiparam’, ‘Hakunohikari’, ‘Charming Eye’ and ‘Moon light’ requires more improved culture conditions. Tissues with the highest shoot regeneration rate were in descending order, leaf, petiole, and internode segments.
Analysis of germinating seed stage expressed sequence tags in Oryza sativa L.
Yoon, Ung-Han ; Lee, Gang-Seob ; Kim, Chang-Kug ; Lee, Jung-Sook ; Hahn, Jang-Ho ; Yun, Doh-Won ; Ji, Hyeon-So ; Lee, Tae-Ho ; Lee, Jeong-Hwa ; Park, Sung-Han ; Kim, Gun-Wook ; Seo, Mi-Suk ; Kim, Yong-Hwan ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 281~288
DOI : 10.5010/JPB.2009.36.3.281
Seed germination is the important stage to express many genes for regulation of energy metabolism, starch degradation and cell division from seed dormancy state. For the functional analysis of seed germination mechanisms, we were analyzed the rice cDNA clones (Oryzasativa cultivar Ilpum) obtained from seed imbibition during 48 hours. Total number of 18,101 Expressed Sequence Tags (ESTs) were clustered using SeqMan program. Among them, 8,836 clones were identified as unique clones. We identified the chitinase gene specifically expressed in seed germination and amylase gene involved to starch degradation from the full length cDNA analysis, and several genes were registered to NCBI GeneBank. To analyzed the commonly expressed genes between inmature seed and germinated seed, 25,66 inmature ESTs and 18,101 germinated ESTs were clustered using SeqMan program and identified 2,514 clones as commonly expressed unigene. Among them, alpha-glubulin and alcohol dehydrogenase I were supposed to LEA genes only expressed in the immature and germinated seed stages. For the clustering of orthologous group genes, we further analyzed the 8,836 EST clones from germinating seeds using NCBI clusters of orthologous groups database. Among the clones, 5,076 clones were categorized into information storage and processing, cellular processes and signaling, metabolism and poorly characterized genes, proportioning 783 (14.29%), 1,484 (27%), 1,363 (24.8%) and 1,869 (34%) clones to the previous four categories, respectively.
Inheritance and expression of transgene in SOD2-Transgenic petunia descendants and their morphological traits
Lee, Su-Young ; Han, Bong-Hee ; Cho, A-Young ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 289~293
DOI : 10.5010/JPB.2009.36.3.289
This study was conducted to determine the inheritance and expression of transgene in descendants (
generation) of SOD2-transgenic petunia by PCR and RT-PCR analysis. The trangene was segregated as Mendelian inheritance pattern (3:1 or 1:0) in most of
generation lines. Transgenic homozygous lines were obtained in T2 generation. It was identified that the transgene expressed stably in examined all plants of 6
lines. The representative morphological traits (plant height, flower diameter, and flower color) of
plants were compared with those of non-transgenic plants.
Mass production and application of activation tagged hairy root lines for functional genomic of secondary metabolism in ginseng
Choi, Dong-Woog ; Chung, Hwa-Jee ; Ko, Suk-Min ; In, Dong-Soo ; Song, Ji-Sook ; Woo, Sung-Sick ; Liu, Jang R. ;
Journal of Plant Biotechnology, volume 36, issue 3, 2009, Pages 294~300
DOI : 10.5010/JPB.2009.36.3.294
Activation tagging that uses T-DNA vectors containing multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene is a powerful tool to determine gene function in plants. This approach has been successfully applied in screening various types of mutations and cloning the corresponding genes. We generated an activation tagged hairy root pool of ginseng (Panax ginseng C.A. Meyer) in an attempt to isolate genes involved in the biosynthetic pathway of ginsenoside (triterpene saponin), which is known as the major active ingredient of the root. Quantitative and qualitative variation of ginsenoside in activation tagged hairy root lines were profiled using LC/MS. Metabolic profiling data enabled selection of a specific hairy root line which accumulated ginsenoside at a higher level than other lines. The relative expression level of several genes of triterpene biosynthetic pathway in the selected hairy root line was determined by real time RT-PCR. Overall results suggest that the activation tagged ginseng hairy root system described in this study would be useful in isolating genes involved in a complex metabolic pathway from genetically intractable plant species by metabolic profiling.