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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 36, Issue 4 - Dec 2009
Volume 36, Issue 3 - Sep 2009
Volume 36, Issue 2 - Jun 2009
Volume 36, Issue 1 - Mar 2009
Selecting the target year
World agricultural crop supplies and Korea`s food security
Chung, Chang-Ho ; Kyung, Kyu-Hang ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 301~308
DOI : 10.5010/JPB.2009.36.4.301
Higher agricultural commodity prices are a particular concern for food importing countries like Korea that has a very low self-sufficiency ratio. Korean people eat approximately 4.5 million metric tons of rice each year, which is met without a problem by domestic production. The domestic production of corn and soybean which are important raw materials for commercial food processing and livestock feed is only minimal. Demands of corn and soybean in Korea are approximately 7.2 million and 1.3 million metric tons per year, respectively. Since Korean consumers are reluctant to accept biotech (GM) foods, Korean food processors are fighting an up-hill battle in purchasing non-biotech (non- GM) crops which are becoming scarce.
Production of biopharmaceuticals in transgenic plant cell suspension cultures
Kwon, Jun-Young ; Cheon, Su-Hwan ; Lee, Hye-Ran ; Han, Ji-Yeon ; Kim, Dong-Il ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 309~319
DOI : 10.5010/JPB.2009.36.4.309
Transgenic plant cell cultures for the production of biopharmaceuticals including monoclonal antibodies, recombinant proteins have been regarded as an alternative platform in addition to traditional microbial fermentation and mammalian cell cultures. Plant-made pharmaceuticals (PMPs) have several advantages such as safety, cost-effectiveness, scalability and possibility of complex post-translational modifications. Increasing demand for the quantity and diversity of pharmaceutical proteins may accelerate the industrialization of PMP technology. Up to date, there is no plant-made recombinant protein approved by USFDA (Food and Drug Administration) for human therapeutic uses due to the technological bottlenecks of low expression level and slight differences in glycosylation. Regarding expression levels, it is possible to improve the productivity by using stronger promoter and optimizing culture processes. In terms of glycosylation, humanization has been attempted in many ways to reduce immune responses and to enhance the efficacy as well as stability. In this review article, all these respects of transgenic plant cell cultures were summarized. In addition, we also discuss the general characteristics of plant cell suspension cultures related with bioreactor design and operation to achieve high productivity in large scale which could be a key to successful commercialization of PMPs.
Current status on Miscanthus for biomass
Seo, Sang-Gyu ; Lee, Jeong-Eun ; Jeon, Seo-Bum ; Lee, Byung-Hyun ; Koo, Bon-Cheol ; Suh, Sae-Jung ; Kim, Sun-Hyung ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 320~326
DOI : 10.5010/JPB.2009.36.4.320
The carbon dioxide concentration of the atmosphere is projected to increase by almost 50% over the first 50 years of this century. The major cause of this increase is continued combustion of fossil fuels. As a result, the significant changes in climate that have already occurred will be amplified, in particular a global temperature increase. Renewable energy production has a central role to play in abating net
emissions to a level that will arrest the development of global warming. Especially, biomass crops are becoming increasingly important as concerns grow about climate change and the need to replace carbon dioxideproducing fossil fuels with carbon-neutral renewable sources of energy. To succeed in this role, biomass crop has to grow rapidly and yield a reliable, regular harvest. A prime candidate is Miscanthus, or Asian elephant grass, a perennial species that produces over 3 metres of bamboo-like stems in a year. Miscanthus species are typically diploid or tetraploid. Hybrids between species with different ploidy levels result in the highly productive triploid hybrids, M.
giganteus. Here we will detail the Miscanthus characteristics desired of a biomass fuel crop.
Recent advances in the development of biotech bentgrass
Hwang, Ok-Jin ; Kim, Jeong-Il ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 327~335
DOI : 10.5010/JPB.2009.36.4.327
Creeping bentgrass (Agrostis stolonifera L.) is economically important as the principal turfgrass species for golf course greens and fairways in temperate climates around the world. As the utilization area of the turfgrass species increases recently, the demand for new and improved cultivars increases. Thus, substantial progress has been made in applying modern biotechnology to develop genetically engineered (i.e., biotech) creeping bentgrass with new traits that eluded the breeders. This review article addresses the advances made in developing biotech creeping bentgrass, which are categorized in the following topics: (i) genetic transformation of creeping bentgrass, (ii) development of various biotech creeping bentgrasses by genetic engineering, and (iii) progresses in the deregulation of herbicideresistant creeping bentgrass.
Recent advance in genetic transformation of tall fescue
Lee, Ki-Won ; Lee, Sang-Hoon ; Kim, Kyung-Hee ; Lee, Byung-Hyun ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 336~343
DOI : 10.5010/JPB.2009.36.4.336
Tall fescue is an open-pollinated, perennial, cool season grass species widely used for forage and turf. Tremendous progress has been made in genetic transformation of tall fescue in the past decade. Methods for generating transgenic tall fescue plants have been developed based on biolistic transformation and Agrobacterium-mediated transformation. Potentially useful agronomic genes have been tested to environmental stress tolerance, herbicide tolerance and improve forage quality in tall fescue plants. We review progress in biotechnological improvement of tall fescue and discuss future molecular breeding of this species.
Development of genetic transformation method of Korean soybean
Jeon, Eun-Hee ; Chung, Young-Soo ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 344~351
DOI : 10.5010/JPB.2009.36.4.344
Current status of soybean transformation method in Korera was reviewed with recent publications. Most frequently used method for genetic transformation was Agrobacterium-mediated transformation on cotyledonary node which is most popular method used in foreign country. In addition to this, various methods such as sonicationmediated transformation, in planta transformation, and transformation on meristem tissue of germinating seed, have been tried in Korea, even though their efficiencies on repeatability and stability were relatively low. Based on the promising results developed recently by reviewer, several important considerations for successful soybean transformations were suggested. They are 1) proper genotype screening, 2) targeting transformation on exact point, 3) multiple shoot formation, 4) efficient selection pressure, 5) successful shoot elongation, 6) efficient root formation. These are the basic requirements for stable and highly efficient soybean transformation of Korean soybean.
Metabolic engineering for production of ginsenosides in Panax ginseng
Kim, Tae-Dong ; Kim, Yun-Soo ; Han, Jung-Yeon ; Lim, Soon ; Choi, Yong-Eui ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 352~359
DOI : 10.5010/JPB.2009.36.4.352
Panax ginseng roots produce triterpene saponins called ginsenosides, which are high value secondary metabolites and has been used as drugs, detergents, sweeteners, and cosmetics. In the recent years plant cell, tissue and organ cultures have developed as important alternative sources for the saponin production in Panax ginseng. Adventitious roots and hairy roots have been successfully induced and cultured for the improvement of saponin contents. Genetic and metabolic engineering to regulate saponin biosynthesis in P. ginseng might be important way to improve the medicinal values of P. ginseng. Here we introduced the protocol of genetic transformation and recent progress of functional characterization of genes involved in saponin biosynthesis in P. ginseng.
Development of genetically modified crops based on considerations of risk assessment and management
Kim, Chang-Gi ; Jeong, Soon-Chun ; Yoon, Won-Kee ; Park, Kee-Woong ; Choi, Kyung-Hwa ; Kim, Hwan-Mook ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 360~365
DOI : 10.5010/JPB.2009.36.4.360
Over the last five years, we have conducted research on risk assessment of domestically developed genetically modified (GM) crops and found a number of factors which could delay risk assessment process. In this review, we described such cases and discussed the problem of transgene cassette integration, the lack of information on vectors, the poor quality control in seed production and absence of bioinformatic analysis on amino acid sequence homology before GM crop development. To solve these problems, we have suggested the introduction of the screening system of elite event before risk assessment process and quality control strategies for GM seed production. In addition, we suggested that the developers of GM crops should understand the importance of risk assessment and management for the commercialization of those crops and consider the biological and ecological characteristics of host plants. Consistent communications may need to be established between GM crop developers, risk assessors and risk managers at the initial stages of GM crop development to reduce trial-and-errors.
MdMADS2 - transgenic chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) showing the reduction of the days to flowering
Han, Bong-Hee ; Lee, Su-Young ; Choi, Seong-Youl ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 366~372
DOI : 10.5010/JPB.2009.36.4.366
This study was conducted to develop new lines expressing the characteristic of early flowering by introducing MdMADS2 gene in chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) ‘Zinba`. Transformation of chrysanthemum was conducted by Agrobacterium tumefaciens LBA4404 harboring the binary vector containing MdMADS2 controlled by double CaMV 35S promoters. Ninety three shoots were regenerated from 1,463 leaf segment explants cultured on the first selection medium (MS basal salts + 1.0 mg/L BA + 0.5 mg/L IAA + 10 mg/L kanamycin + 400 mg/L cefotaxime, pH 5.8) after co-cultivation, and 20 out of the 93 shoots rooted on the second selection medium containing 20 mg/L kanamycin and 400 mg/L cefotaxime. Many escapes (98.6%) were removed on the selection stage for rooting. Nineteen lines were confirmed as transgenic plant with transgene by PCR analysis. Six transgenic plants flowered 2-11 days earlier than non-transgenic plant without big change of phenotype, and especially, 3 (Mo-7, Mo-11, Mo-17) out of 6 transgenic lines showed a significant reduction in days to flowering compared to non-transgenic plant. Introduction and expression of MdMADS2 gene in them were confirmed by Southern and real-time PCR analyses, respectively.
Selection of the fittest varieties of chrysanthemum (Dendranthema grandiflorum Kitamura) and set of culture condition for efficient transformation
Kang, Chan-Ho ; Yun, Seung-Jung ; Han, Bum-So ; Han, So-Gon ; Kown, Sung-Hwan ; Song, Young-Ju ; Jang, Mi-Hyang ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 373~383
DOI : 10.5010/JPB.2009.36.4.373
To set efficient transformation system in chrysanthemum, thirty-four chrysanthemum (Dendranthema grandiflorum Kitamura) varieties were collected and cultured for shoot regeneration. Five varieties, ‘Shuho-no-chikara`, ‘Zinba`, ‘Baekma`, ‘Pink pride` and ‘Keumsu` of them were selected, because they had a high shoot regeneration efficiency. MS medium containing 1.0 mg/L NAA and BA respectively was very adequate for shoot regeneration in those varieties. MS medium with 3.0 mg/L NAA and 1.0 mg/L kinetin in ‘Shuho-no-chikara` and the medium with 0.5 mg/L NAA and 3.0 mg/L BA in ‘Keumsu` were also suitable for shoot regeneration. The most efficient callus induction and shoot regeneration were obtained on MS medium. Shoot regeneration was enhanced more than 8% on MS medium with 0.3% phytagel and 10-15 mg/L putrescine. The best cultural material for shoot regeneration was stem. When stem was used as a culture material, shoot regeneration rate was increased more than 26% and the days to shoot regeneration was shortened about 14 days.
Characterization, detection and identification of transgenic chili pepper harboring coat protein gene that enhances resistance to cucumber mosaic virus
Seo, Sang-Gyu ; Kim, Ji-Seong ; Jeon, Seo-Bum ; Shin, Mi-Rae ; Kang, Seung-Won ; Lee, Gung-Pyo ; Hong, Jin-Sung ; Harn, Chee-Hark ; Ryu, Ki-Hyun ; Park, Tae-Sung ; Kim, Sun-Hyung ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 384~391
DOI : 10.5010/JPB.2009.36.4.384
Previously, two events (H15 and B20) of transgenic pepper (Capsicum annuum L.) that enhanced resistance to Cucumber mosaic virus (CMV) by the introduction of CMV coat protein (CP) gene were constructed. Presently, a single copy number of the CP gene was revealed in H15 and B20 by Southern blot. To predict possible unintended effects due to transgene insertion in an endogenous gene, we carried out sequencing of the 5`-flanking region of the CP gene and a Blastbased search. The results revealed that insertion of the transgene into genes encoding putative proteins may occur in the H15 and B20 transgenic event. Mutiplex polymerase chain reaction (PCR) for simultaneous detection and identification of transgenic pepper was conducted with a set of nine primers. Both transgenic event were differentiated from non-transgenic event by the presence of 267 bp and 430 bp PCR products indicative of CP gene specific primer pairs and primer pairs targeting the CP gene and 35S promoter. H15 and B20 uniquely possessed a 390 bp and 596 bp PCR product, respectively. The presence of a 1115 bp product corresponding to intrinsic pepper actin gene confirmed the use of pepper DNA as the PCR template. The primer set and PCR conditions used presently may allow the accurate and simple identification of CMV resistant transgenic pepper.
Mass production of the seedlings of Dendrobium moniliforme using bioreactor culture
Whang, Sung-Soo ; Koo, Ja-Choon ; Choi, Kyung ; Park, Kwang-Woo ; Kang, Kyung-Won ; Choi, Eun-Gyung ; Kim, Jae-Whune ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 392~396
DOI : 10.5010/JPB.2009.36.4.392
Protocorms were newly formed from the culture of axillary buds, obtained in the seedlings of Dendrobium moniliforme in vitro. Its formation ratio was calculated to 43.7% on MS medium containing 1.0 mg/L BA. To test their survival ratio, we gradually increased the inoculation of transplant populations from single to more than three, and then found that the ratio in three populations went up as high as 95.2% rather than those of one or two. In bioreactor, explant obtained from the axillary bud grew well in lower concentration as 1/4 MS medium, while clearly grew slow in a little bit high concentration as 1/2 MS medium. We found that the explant of axillary bud, obtained from the Dendrobium moniliforme seedlings, would grow five times after culturing in a bioreactor for six weeks in 1/4 MS medium.
Characterization of a gene encoding ornithine carbamoyltransferase from rice
Islam Sikdar, Shafiqul ; Kim, Jung-Sup ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 397~402
DOI : 10.5010/JPB.2009.36.4.397
Ornithinine carbamoyltransferase (OTC) is an enzyme that catalyzes the key step in arginine biosynthesis in bacteria and plants. OTC is also involved in the urea cycle and deficiency of the enzyme in human leads to disease. The argF gene encoding OTC has been reported in many bacteria and few plants. Here we report the characterization of a gene encoding OTC from rice (OsOTC). Analysis of a cDNA sequence from rice revealed that the full-length open reading frame of OsOTC consisted of 367 amino acids, corresponding to a protein of approximately 39.7 kDa. The predicted amino acid sequence of OsOTC harbor distinct five OTC signature sites and is highly homologous to that of enzymes of plants, animals and many bacterial OTCs. Expression of OsOTC in argF mutants of Escherichia coli showed that the gene was able to functionally complement to the mutant. These results suggest that the OsOTC encode a protein for ornithine carbamoyltransferase in rice.
Plant regeneration from callus derived root of northen type in garlic (Allium sativum L.)
Ahn, Yul-Kyun ; Kim, Do-Sun ; Yoon, Moon-Kyoung ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 403~406
DOI : 10.5010/JPB.2009.36.4.403
This study was conducted to develop an effective production of callus induction and plant regeneration system for garlic transformation. The best callus production occurred on in vitro root segment initially cultured on MS medium with 1.0 mg/L 2,4-D and 0.2 mg/L IAA in both ‘Danyang` and ‘Euseong`. The frequency of callus formation were 81.2% ‘Danyang` and 76.1% ‘Euseong`. Eight weeks after callus induction, callus lines were transferred to regeneration medium during 7 weeks. The best shoot regeneration medium was MS supplemented with 5 mg/L Kinetin and 1 mg/L NAA for ‘Danyang` and MS supplemented with 10 mg/L BAP for ‘Euseong`. The frequency of shoot regeneration were 51.5% ‘Danyang` and 56.6% ‘Euseong` The plantlets were acclimatized and transferred to the greenhouse with almost survival. This in vitro regeneration system should be useful for garlic transformation.
Isolation and characterization of Brcpi1 gene encoding phytocystatin from chinese cabbage (Brassica rapa L.) seedlings
Jung, Yu-Jin ; Cho, Yong-Gu ; Kang, Kwon-Kyoo ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 407~414
DOI : 10.5010/JPB.2009.36.4.407
A cDNA clone encoding phytocystatin was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (name as Brcpi1; GenBank accession no.: EF079953) had a total length of 881 bp with an open reading frame of 609 bp, and encoded predicted polypeptide of 203 amino acid (aa) residues including a putative N-terminal signal peptide. Other relevant regions found its sequence included the G and PW conserved aa motifs, and the consensus LARFAV sequence for phytocystatins and the reactive site QVVAG. The BrCPI1 protein shared 95, 94, 81, 80 and 78% identity with other CPI proterins isolated from Brassica oleracea (BoCPI-1), Arabidopsis thaliana (AtCY SB), Glycine max (GmCPI), Oryza sativa (OsCYS-2) and Zea may (ZmCPI) at amino acid level, respectively. Southern blot analysis showed that Brcpi1 was a low copy gene. Expression pattern analysis revealed that Brcpi1 was a tissue-specific expressing gene during reproductive growth and strongly expressed at mature seedling stages. Furthermore, overexpression of Brcpi1 in transgenic Arabidopsis was enhanced tolerance to salt and cold stresses. Meanwhile the juvenile seedling of Brcpi1 transgenic plants was not affected by various concentrations ABA in MS medium. Taken together, the results showed that Brcpi1 functioned as a cysteine protease inhibitor and it exhibited a protective agent against diverse types of abiotic stress, which induced this gene in a tissue- and stress-specific manner.
Characterization of flavonoids specific gene expression in the petals of Dianthus caryophyllus (carnation)
Hur, Suel-Hye ; Ahn, Byung-Joon ; Joung, Hyang-Young ; Hyung, Nam-In ; Min, Byung-Whan ;
Journal of Plant Biotechnology, volume 36, issue 4, 2009, Pages 415~422
DOI : 10.5010/JPB.2009.36.4.415
This study aimed to develop carnation cultivars with new coloring system. We used four genes of Petunia hybrida - chalcone synthase (CHS), flavanone 3-hydroxylase (FHT), dihydroflavonol 4-reductase (DFR), and anthocyanidin synthase (ANS) - as probes, in order to isolate four genes from carnations (Dianthus Caryophyllus). The isolated genes were used as probes in order to select mutants out of collected carnations, using Northern blot analysis. The Northern blot analysis revealed 10 DFR mutants - Gumbyul, Eunbyul, Ballatyne, Crystal, Eugenia, Koreno, Imp. White Sim, West Crystal, White Alpine, and White Charotte. Six among the selected 10 cultivarswere excluded from the target cultivars, because Eugenia, Imp. White Sim, and White Alpine were proved to be double mutants of DFR and ANS, Koreno was considered to be a double mutant of DFR and CHS, and Gumbyul and Ballatyne were proved to be double mutants of DFR and CHI (Chalcone isomerase). Consequently, we selected five DFR mutants, including Virginie, which was already selected as a DFR mutant. Finally, we measured DFR activities in order to confirm the selection, and the results showed that all of the five cultivars - Eunbyul, Crystal, West Crystal, White Charotte, and Virginie - had got no DFR activity.