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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 37, Issue 4 - Dec 2010
Volume 37, Issue 3 - Sep 2010
Volume 37, Issue 2 - Jun 2010
Volume 37, Issue 1 - Mar 2010
Selecting the target year
Improvement of floral scent of ornamentals via metabolic engineering
Kang, Seung-Won ; Seo, Sang-Gyu ; Ryu, So-Young ; Pak, Chun-Ho ; Lee, Gung-Pyo ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 1~11
DOI : 10.5010/JPB.2010.37.1.001
Floral scent emitted from many plants is the key factor for pollinator attraction and defense for survival in nature and is important industrial materials for perfumery as well. It is a complex mixture of various organic molecules with a high volatility or a high vapor pressure. In general, floral scents are divided into three categories, aliphatics, terpenoids, and phenylpropanoids/benzenoids, based on its origin. About 1,700 scent compounds have been identified and their biochemistry and molecular biology also have elucidated their biosynthesis from various flowering plants during the last ten years. In addition to improvement of vase life, flower color and shape, and/or disease resistance, floral scent is coming up to the major breeding target for improvement of marketability. Therefore, metabolic engineering can be an important tool in near future and may be able to facilitate the breeding program for novel cultivar selection and improvement of marketability of floricultural crops.
Present and prospect of plant metabolomics
Kim, Suk-Weon ; Kwon, Yong-Kook ; Kim, Jong-Hyun ; Liu, Jang-R. ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 12~24
DOI : 10.5010/JPB.2010.37.1.012
Plant metabolomics is a research field for identifying all of the metabolites found in a certain plant cell, tissue, organ, or whole plant in a given time and conditions and for studying changes in metabolic profiling as time goes or conditions change. Metabolomics is one of the most recently developed omics for holistic approach to biology and is a kind of systems biology. Metabolomics or metabolite fingerprinting techniques usually involves collecting spectra of crude solvent extracts without purification and separation of pure compounds or not in standardized conditions. Therefore, that requires a high degree of reproducibility, which can be achieved by using a standardized method for sample preparation and data acquisition and analysis. In plant biology, metabolomics is applied for various research fields including rapid discrimination between plant species, cultivar and GM plants, metabolic evaluation of commercial food stocks and medicinal herbs, understanding various physiological, stress responses, and determination of gene functions. Recently, plant metabolomics is applied for characterization of gene function often in combination with transcriptomics by analyzing tagged mutants of the model plants of Arabidopsis and rice. The use of plant metabolomics combined by transcriptomics in functional genomics will be the challenge for the coming year. This review paper attempted to introduce current status and prospects of plant metabolomics research.
Review of property and utilization of oil crop for biodiesel
Jang, Young-Seok ; Kim, Kwang-Soo ; Lee, Yong-Hwa ; Cho, Hyeon-Jun ; Suh, Sae-Jung ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 25~46
DOI : 10.5010/JPB.2010.37.1.025
The demand for fuel and energy resources continues to grow due to increased consumption and emerging economies in all parts of the world. With this increase in demand, crude oil prices in the international market has jumped dramatically. Global warming, which is a consequence of increasing greenhouse gas (GHG) emissions, has become scientific, social, and political concerns. To cope with global warming and energy crisis, cost-competitive biofuels are urgently needed. In addition, development of an infrastructure, which supplies energy stably and diversifies energy resources, as well as new cost-saving technologies should be developed to reduce the costs of producing biofuels. Due to high oleic acid content, rapeseed (Brassica napus L.) is currently the potential feedstock for biodiesel production in temperate zone region and the production and use of rapeseed oil is already commercialized in Europe. In Korea double-cropping (rice and rapeseed) became more prevalent because it reduces competitions from land constraints. Production of rapeseed as a biodiesel feedstock may reduce the influence of rising oil prices and nation`s dependence on imported petroleum and increase job opportunities and farm incomes.
Phamalogical effect and component of sea buckthorn(Hippophae rhamnoides L.)
Kim, Ju-Sung ; Yu, Chang-Yeon ; Kim, Myong-Jo ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 47~56
DOI : 10.5010/JPB.2010.37.1.047
Sea buckthorn (Hippophae rhamnoides L.) is deciduous shrubs in the genus Hippophae, mainly cultivated in Europe and Asia. Sea buckthorn berries have a high vitamin C, vitamin E, carotenoids, carbohydrates, protein, organic acids, dietary minerals, triterpenoids, polyphenolic acids and amino acids. Extracts of sea buckthorn berries have anti- obesity, anti-oxidantive, anti-microbial, anti-ulcerogenic, anti-diabetic and nutritional effects. Sea buckthorn used as a traditional medicine for the treatment of cough, aid digestion, invigorate blood circulation and alleviate pain. Extracts of sea buckthorn branches and leaves was administered to humans and animals to treat gastrointestinal distress in Mongolia. This paper briefly reviews the most relevant experimental data on the pharmacological effects and isolated component of sea buckthorn. And, we also describe the importance of sea buckthorn as the environmental-friendly crops.
Autophagy: Noble target mechanisms in natural medicines as anticancer agents
Kang, Se-Chan ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 57~66
DOI : 10.5010/JPB.2010.37.1.057
Programmed cell death systems are important for an active type of cell deaths. Among them, a type of programmed cell death, autophagy is activated in cancer cells in response to multiple stresses and has been demonstrated to promote tumor cell survival and drug resistance. Thus, in the area of cancer, over the time frame form around the 1940s to date, of the 155 small molecules, 73% are other than "synthetic", with 47% actually being either "natural products" or "directly derived therefrom". Autophagy has multiple physiological functions in multicellular organisms, including protein degradation and organelle turnover. Genes and proteins that constitute the basic machinery of the autophagic process were first identified in the yeast system and some of their mammalian orthologues have been characterized as well. Numerous oncogenes, including Akt1, Bcl-2, NF1, PDPK1, class I PI3K, PTEN, and Ras and oncosuppressors, inculuding Bec-1, Bif-1, DAPK-1, p53 and UVRAG suppress or promote the autophagy pathway. Regulation of autophagy in tumors is governed by similar principles of the normal cells, only in a much more complicated manner, given the frequently observed abnormal PI3K activation in cancer and the multitude of interactions between the PI3K/AKT/mTOR pathway and other cell signaling cascades, often also deregulated in tumor cells. Autophagy induction by some anticancer agents underlines the potential utility of its induction as a new cancer treatment modality of development for natural medicines.
Output traits in crop plants: Nutrients and pharmaceuticals
Yu, Ju-Kyung ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 67~71
DOI : 10.5010/JPB.2010.37.1.067
Output traits centered on improved plant-based products will find their way to consumers in such ways as nutritionally enhanced foods, therapeutic proteins for disease treatment and vaccines, bio-industrial products, modified oil quality and biofuels. Significant progress in biotechnology has occurred over the last several decades. The importance of output traits development and production using biotechnology will impact not only agribusiness, but also pharmaceutical and food industries. The objective of this paper is to review briefly the current status of output traits development in crop plants using nutrients and pharmaceuticals as examples.
Development of transgenic cucumber expressing TPSP gene and morphological alterations
Kim, Hyun-A ; Min, Sung-Ran ; Choi, Dong-Woog ; Choi, Pil-Son ; Hong, Seong-Gyun ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 72~76
DOI : 10.5010/JPB.2010.37.1.072
To develop transgenic cucumber tolerant to abiotic stress, a cotyledonary-node explants were co-cultivated with Agrobacterium tumefaciens (EHA101) carrying TPSP gene (pHC30-TPSP). After transfer to fresh medium every two week for eight weeks, putative transgenic plants were selected when shoots grown a length greater than 3 cm from the cotyledonary-node explants on selection medium supplemented with
phospinotricin as selectable agent. The confirmation of transgenic cucumber was based on the Northern blot analysis. Thirty four shoots (5.2%) with resistance to phospinotricin were obtained from 660 explants inoculated. Of them, transformants were only confirmed from 11 plants (1.7%). Transgenic cucumber expressing TPSP gene was more synthesized at 3.8 times amounts of trehalose (0.014 mg g fresh
) than non-transformants (0.0037 mg g fresh
). However, all of transgenic plants showed abnormal morphology, including stunted growth (< height 15 cm), shrunken leaves, and sterility as compared with non-transgenic plants (> height 150 cm) under the same growth environment. These results lead us to speculate that the overproduction of trehalose was toxic for cucumber, even though that had known for rice as non-toxic.
Effect of cultivar and ascorbic acid on in vitro shoot regeneration and development of bombardment-mediated plastid transformation of tomato (Lycopersicon esculentum)
Roh, Kyung-Hee ; Lee, Ki-Jong ; Park, Jong-Sug ; Kim, Jong-Bum ; Lee, Seung-Bum ; Suh, Seok-Cheol ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 77~83
DOI : 10.5010/JPB.2010.37.1.077
Eighteen cultivars of tomato were tested for their regeneration response. Lycopersicon esculentum cv. 2001-58 showed a very high frequency of regeneration (93%). We evaluated the effect of two compounds with known antioxidant activity (ascorbic acid and cystein). The use of ascorbic acid (
) has a positive effect on shoot regeneration. To develope a system for plastid transformation in tomato via homologous recombination, we constructed the tomato plastid expression vector (pKRT22-AG) harboring 2.2 kb flanking sequences cloned from intact plastid genome and gfp gene. To investigate the factors affecting the delivery system of the pKRT22-AG into chloroplast using bombardment, We assessed the optimal DNA concentration, gold particle volume and target distance. Expression of the GFP protein was observed within chloroplast on protoplast of cotyledon explant by confocal laser scanning microscopy, which indicates that the protocol developed in this study be useful for the production of plastid transgenic plants in tomato.
Effect of plant growth regulators on plant regeneration from the Sedum rotundifolium D. Lee
Kwon, Hye-Kyoung ; Yoon, Eui-Soo ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 84~88
DOI : 10.5010/JPB.2010.37.1.084
To establish the system of In vitro plant regeneration, the floral bud and leaf explants of Sedum rotundifolium were cultured on the MS media supplemented with different concentration of 2,4-D, NAA, and BA. The callus induction was more effective in the floral explants than the leaf explants, and was the best on MS medium containing 1.0 or 2.0 mg/L 2,4-D and 1.0 mg/L BA. The highest numbers of shoots were regenerated when callus were cultured on MS medium containing 2.0 mg/L 2,4-D and 1.0 mg/L BA for 8 weeks. The normal root formation from shoot was effective on the MS medium containing IAA alone. The regenerated plantlets were transferred to the pot and acclimatized successfully.
Molecular cloning, sequences analysis and in vitro expression of the dihydroflavonol 4-reductase gene from Gypsophila paniculata L.
Min, Byung-Whan ; Cheong, Dong-Chun ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 89~95
DOI : 10.5010/JPB.2010.37.1.089
Dihydroflavonol 4-reductase (DFR) is a key enzyme of the flavonoid biosynthesis pathway which catalyses the NADPH-dependent reduction of 2R,3R-trans-dihydroflavonols to leucoanthocyanidins. In this study we describe cloning and expression of the genes encoding the flavonoid-biosynthetic enzyme DFR in Gypsophila paniculata L. Inspection of the 1279 bp long sequence revealed an open reading frame 1063 bp, including a 36 bp 5` leader region and 181 bp 3` untranslated region. Comparison of the coding region of this DFR cDNA sequence including the sequences of Arabidopsis thaliana, Citrus sinensis, Dianthus caryophyllus, Ipomoea batatas, Matthiola incana, Nierembergia sp, Petunia hybrida, Solanum tuberosum, Vitis vinifera reveals an identity higher than 69% at the nucleotide level. The function of this nucleotide sequences was verified by comparison with amino acid sequences of the amino-terminus and tryptic peptides from purified plant enzyme, by northern blotting with mRNA from wild type and mutant plants, by in vitro expression yielding and enzymatically active reductase, as indicated by the small leucopelargonidin peak. Genomic southern blot analysis showed the presence of only one gene for DFR in Gypsophila paniculata.
Production of glycoalkaloids from callus cultures of Solanum hainanense Hance
Loc, Nguyen Hoang ; Anh, Nguyen Huu Thuan ; Binh, Doan Huu Nhat ; Yang, Moon-Sik ; Kim, Tae-Geum ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 96~101
DOI : 10.5010/JPB.2010.37.1.096
Leaf explants of the Solanum hainanense plant, grown in vitro, were cultured in basal Murashige and Skoog (MS) media supplemented with 0.5 mg/L kinetin and 1 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) for callus initiation. For maintenance and proliferation, the callus was cultured on MS medium supplemented with 1 mg/L 6-benzylaminopurine (BAP) and 0.5 mg/L 2,4-D. The glycoalkaloid content in the callus was at its maximum after ten weeks of culture (188.65 mg/g), whereas that of the one-year-old control was 22.22 mg/g in the root and 5.99 mg/g in the stem. The glycoalkaloid extracted from the callus inhibited the activity of collagenase on collagen gel. High performance liquid chromatography (HPLC) analysis showed that biotransformation occurred when a callus was grown on medium supplemented with various carbon sources. These results suggest that callus of S. hainanense is a good material for production of glycoalkaloid.
Increment of fructan biosynthesis in rice by transformation of 1-sst and 1-fft genes isolated from jerusalem artichoke (Helianthus tuberosus L.)
Kang, Kwon-Kyoo ; Song, Beom-Heon ; Lee, Gyong-A ; Lee, Hye-Jung ; Park, Jin-Ha ; Jung, Yu-Jin ; Cho, Yong-Gu ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 102~109
DOI : 10.5010/JPB.2010.37.1.102
Fructan has been found to accumulate in various tissues during periods when light levels increased carbon fixation where low temperatures reduced growth rates while photosynthesis continued. In this study, we have cloned 1-sucrose:sucrose fructosyl transferase(1-sst) and 1-fructan: fructan fructosyl transferase (1-fft, a key enzyme for the synthesis of fuctan) from Jerusalem Artichoke (Helianthus tuberosus L.). The recombinant vector with 1-sst and 1-fft has been constructed under the control of 35S promoter of KJGV-B2 vector and transgenic plants obtained by Agrobacterium tumefaciens LBA4404. PCR analysis carried out on the putative transgenic plants for amplification of the coding region of specific gene (1-sst, 1-fft), and HPT genes. Transgenic lines carrying of 1-sst and 1-fft were confirmed for integration into the rice genome using Southern blot hybridization and RT-PCR. The transgenic plants in
generation were selected and expression pattern analysis revealed that 1-sst and 1-fft were stable. This analysis confirmed the presence of low-molecular-weight fructan in the seedling of the transgenic rices. Therefore, cold tolerance and carbohydrate metabolism will be possible to develop resistant plants using the transgenic rice.
Mass production of potato microtubers by bioreactor culture
Kim, Jae-Whune ; Choi, Eun-Gyung ; Oh, Seung-Cheol ; Joo, Sun-Ah ; You, Dong-Min ; Kim, Soon-Kap ; Kim, Jeong-Kook ;
Journal of Plant Biotechnology, volume 37, issue 1, 2010, Pages 110~114
DOI : 10.5010/JPB.2010.37.1.110
Highest increase of biomass was observed when tissue-cultured potato (Solanum tuberosum L. cv. Chubaek) shoots were cultured in a liquid medium containing 1/3 MS solution in a 18 L bioreactor, as compared to 1/4 and 1/2 MS solution. The medium containing 1/4 MS solution showed higher increase of shoot biomass than one containing 1/2 MS solution. Potato microtubers were formed when the medium was exchanged with the medium for microtuber formation and incubated under dark condition. The microtubers were observed first at some axillary buds one week after incubation under dark condition and then at most of the axillary buds by the end of 3 weeks. The 1.5 MS liquid medium and
were optimal conditions. By the end of 6 weeks, more 1,000 microtubers were formed in the 18 L bioreactor. Then, greened microtubers were harvested after one week culture under light condition.