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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 38, Issue 4 - Dec 2011
Volume 38, Issue 3 - Sep 2011
Volume 38, Issue 2 - Jun 2011
Volume 38, Issue 1 - Mar 2011
Selecting the target year
Current biotechnology for the increase of vegetable oil yield in transgenic plants
Lee, Kyeong-Ryeol ; Choi, Yun-Jung ; Kim, Sun-Hee ; Roh, Kyung-Hee ; Kim, Jong-Bum ; Kim, Hyun-Uk ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 241~250
DOI : 10.5010/JPB.2011.38.4.241
The most part of vegetable oils is accumulated as storage lipid, triacylglycerol (TAG) in seed and used as energy source when seed is germinated. It is also used as essential fatty acids and energy source for human and animal. Recently, vegetable oils have been more and more an important resource because of the increasing demand of vegetable oils for cooking and industrial uses for bio-diesel and industrial feedstock. In order to increase vegetable oils using biotechnology, over-expressing or repressing the regulatory genes involved in the flow of carbon into lipid biosynthesis is critical during seed development. In this review, we described candidate genes may influence oil amount and investigate their potential for oil increase. Genes involved in the regulation from biosynthesis of fatty acids to the accumulation oils in seed can be classified as follows: First, genes play a role for synthesis precursor molecules for TAG. Second, genes participate in fatty acid biosynthesis and TAG assembly. Lastly, genes encodes transcription factors involved in seed maturation and accumulation of seed oil. Because factors/genes determining oil quantity in seed is complex as mentioned, recently regulation of transcription factors is being considered more favorable approach than manipulate multiple genes for increasing oil in transgenic plants. However, it should be figured out the problem that bad agricultural traits induced by the overexpression of transcription factor gene.
High-efficiency and Rapid Agrobacterium-mediated genetic transformation method using germinating rice seeds
Lee, Hye-Jung ; Abdula, Sailila E. ; Jee, Moo-Geun ; Jang, Dae-Won ; Cho, Yong-Gu ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 251~257
DOI : 10.5010/JPB.2011.38.4.251
Rice is the most important crop as a model plant for functional genomics of monocotyledons. Rice is usually transformed using Agrobacterium tumefaciens. However, the transformation efficiency using previous method is still low. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the inoculation and co-cultivation step. We directly inoculated Agrobacterium containing a CIPK15 gene under the control of CaMV 35S promoter and NOS terminator in the pCAM1300 vector into the pre-soaked seeds in N6D media for 24 hours. After 7 days of culture at
, calli were formed on seeds cultured on the co-cultivation medium containing an antioxidant compound (1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (3 mg/L silver nitrate). We obtained 35 and 22 transgenic plants in rice cultivars, Gopumbyeo and Ilpumbyeo, with increase of transformation efficiency by 30.4% and 22.6%, respectively compared to the general transformation method. The new method in this study would lead to reduction of substantial labor and time to generate transgenic plants.
Anomalous somatic embryos formation and plant regeneration from the cultures of immature embryos of Camellia japonica L.
Choi, Jong-Hye ; Kwon, Suk-Yoon ; Choi, Pil-Son ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 258~262
DOI : 10.5010/JPB.2011.38.4.258
Embryogenic callus was induced from the cultures of immature embryos of Camellia japonica L. on Murashige & Skoog's (MS) solid medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D), and then the embryogenic callus was proliferated on same medium for 4 weeks over. The embryogenic callus was sub-cultured on MS basal medium without 2,4-D to produce coyledonary stage of somatic embryo. The frequency (%) of somatic embryogenesis was 25.1%, and the majority of somatic embryos formed had a abnormal morphology with cupshaped cotyledon (48.3%), one cotyledon (12.6%), three cotyledons (9.4%), four cotyledons (1.9%), whereas was only normal morphology with two cotyledon (27.5%). When the somatic embryos with normal or abnormal cotyledons transfer to MS basal medium or
MS medium with/or without plant growth regulators (
, IBA) for regeneration, the frequency (%) of two-cotyledon embryos regenerated into plantlets was higher 11.1% than one cotyledon (0.0~8.3 %), three cotyledons (0.0~5.8%), four cotyledons (0.0%), cup-shaped (0.3~4.2%). These results demonstrated that the anomalous cotyledons of somatic embryos could caused to decrease the rate of plant regeneration.
New design of rice seed storage proteins
Kim, Young-Mi ; Lee, Jong-Yeol ; Yoon, Ung-Han ; Choi, Sang-Bong ; Ha, Sun-Hwa ; Lim, Sun-Hyung ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 263~271
DOI : 10.5010/JPB.2011.38.4.263
Rice is one of the most important food crops since it is consumed by approximately 60% of the world's population. The most abundant component of rice grain is starch that is an important source of energy. The second abundant component is protein, which is an important protein source for people in many developing countries that rarely take animal protein. However, the rice protein lacks the essential amino acid lysine. Therefore, nutritional improvement in the essential amino acid composition of rice proteins is required. On the other side, rice grain has attracted attention as a diet and health food in developed countries, because its proteins have superior physiological and food processing properties. Thus, nutritional improvements in rice seed proteins by changing amino acid composition or introducing an useful protein or peptide have been studied. This review aims at assessing the current research status of biosynthesis, accumulation, genetic improvement of seed storage proteins by mutation or genetic engineering in rice.
Overproduction of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) confers resistance to the herbicide glyphosate in transgenic rice
Lee, Soo-In ; Kim, Hyun-Uk ; Shin, Dong-Jin ; Kim, Jin-A ; Hong, Joon-Ki ; Kim, Young-Mi ; Lee, Yeon-Hee ; Koo, Bon-Sung ; Kwon, Sun-Jong ; Suh, Seok-Chul ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 272~277
DOI : 10.5010/JPB.2011.38.4.272
Plants expressing Agrobacterium sp. strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) are known to be resistant to glyphosate, a potent herbicide that inhibits the activity of the endogenous plant EPSPS. In order to develop herbicide-resistant rice, we prepared transgenic rice plants with CP4 EPSPS gene under the control of CaMV 35S promoter for over-expression. A recombinant plasmid was transformed into rice via Agrobacterium-mediated transformation. A large number of transgenic rice plants were obtained with glyphosate and most of the transformants showed fertile. The integration and expression of CP4 EPSPS gene from regenerated plants was analyzed by Southern and northern blot analysis. The transgenic rice plants had CP4 EPSPS enzyme activity levels more than 15-fold higher than the wild-type plants. EPSPS enzyme activity of transgenic rice plants was also identified by strip-test method. Field trial of transgenic rice plants further confirmed that they can be selectively survived at 100% by spay of glyphosate (Roundup
) at a regular dose used for conventional rice weed control.
Overexpression of AtCAF1, CCR4-associated factor 1 homologue in Arabidopsis thaliana, negatively regulates wounding-mediated disease resistance
Kwon, Tack-Min ; Yi, Young-Byung ; Nam, Jae-Sung ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 278~284
DOI : 10.5010/JPB.2011.38.4.278
The CCR4-CAF1-NOT complex-mediated degradation of mRNA is a fundamental aspect of gene regulation in eukaryotes. We herein examined the role of AtCAF1 in the innate immune and wound responses of plants. Our results showed that overexpression of AtCAF1 significantly downregulated the transcript level of EFR but not FLS2 and BRI1, as well as abolished up-regulated expression pattern of EFR in response to wounding. Consistently, Agrobacteriummediated transient expression of GUS was highly enhanced in the transgenic plants overexpressing AtCAF. Furthermore, JA responsive genes were down-regulated by overexpression of AtCAF, causing the transgenic plants overexpressing AtCAF more susceptible to necrotrophic fungal pathogen, Botrytis cinerea. These results suggest that The CCR4-CAF1-NOT complex-mediated degradation of mRNA negatively regulates wounding-mediated disease resistance in Arabidopsis thaliana.
MACROPHYLLA/ROTUNDIFOLIA3 gene of Arabidopsis controls leaf index during leaf development
Jun, Sang-Eun ; Chandrasekhar, Thummala ; Cho, Kiu-Hyung ; Yi, Young-Byung ; Hyung, Nam-In ; Nam, Jae-Sung ; Kim, Gyung-Tae ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 285~292
DOI : 10.5010/JPB.2011.38.4.285
In plants, heteroblasty reflects the morphological adaptation during leaf development according to the external environmental condition and affects the final shape and size of organ. Among parameters displaying heteroblasty, leaf index is an important and typical one to represent the shape and size of simple leaves. Leaf index factor is eventually determined by cell proliferation and cell expansion in leaf blades. Although several regulators and their mechanisms controlling the cell division and cell expansion in leaf development have been studied, it does not fully provide a blueprint of organ formation and morphogenesis during environmental changes. To investigate genes and their mechanisms controlling leaf index during leaf development, we carried out molecular-genetic and physiological experiments using an Arabidopsis mutant. In this study, we identified macrophylla (mac) which had enlarged leaves. In detail, the mac mutant showed alteration in leaf index and cell expansion in direction of width and length, resulting in not only modification of leaf shape but also disruption of heteroblasty. Molecular-genetic studies indicated that mac mutant had point mutation in ROTUDIFOLIA3 (ROT3) gene involved in brassinosteroid biosynthesis and was an allele of rot3-1 mutant. We named it mac/rot3-5 mutant. The expression of ROT3 gene was controlled by negative feedback inhibition by the treatment of brassinosteroid hormone, suggesting that ROT3 gene was involved in brassinosteroid biosynthesis. In dark condition, in addition, the expression of ROT3 gene was up-regulated and mac/rot3-5 mutant showed lower response, compare to wild type in petiole elongation. This study suggests that ROT3 gene has an important role in control of leaf index during leaf expansion process for proper environmental adaptation, such as shade avoidance syndrome, via the control of brassinosteroid biosynthesis.
Optimization of particle gun-mediated transformation system in Cymbidium
Noh, Hee-Sun ; Kim, Mi-Seon ; Lee, Yu-Mi ; Lee, Yi-Rae ; Lee, Sang-Il ; Kim, Jong-Bo ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 293~300
DOI : 10.5010/JPB.2011.38.4.293
This study is conducted to develop an efficient transformation system via particle bombardment with PLBs (Protocorm-like bodies) in Cymbidium. For this, pCAMBIA3301 vector which carries a herbicide-resistant bar gene and gus gene as a reporter gene was used for transformation with Cymbidium cultivars 'Youngflower
masako' line. To select transformants, proper concentration of herbicide, PPT (phosphinotricin), should be determined. As a result, 5 mg/l of PPT was selected as a proper concentration. Further, proper conditions for particle bombardment were determined to obtain a high frequency of transformation. Results showed that 1.0
of DNA concentration, 1,100 and 1,350 psi for helium gas pressure, 1.0
of gold particle and 6 cm of target distance showed the best result for the particle bombardment experiment. Also, pre-treatment with combination 0.2 M sorbitol and 0.2 M mannitol for 4 hrs prior to genetic transformation increased the transformation efficiency up to 2.5 times. Using transformation system developed in this study, 3.2 ~ 4.0 transgenic cymbidium plants can be produced from 100 bombarded PLBs on average. Putative transgenic plants produced in this system confirmed the presence of the bar gene by PCR analysis. Also, leaves from randomely selected five transgenic lines were applied for Basta solution (0.5% v/v) to check the resistance to the PPT herbicide. As a result, three of them showed resistance and one of them showed the strongest resistance with the maintenance of green color as non-transformed plants showed. Using this established transformation system, more genes of interests can be introduced into Cymbidium plants by genetic transformation in the future.
Molecular identification of medicinal herbs, Oldenlandia diffusa and Oldenlandia corymbosa based on nrDNA ITS region sequence
Sun, Yan-Lin ; Wang, Dong ; Yeom, Myung-Hun ; Kim, Duck-Hee ; Kim, Han-Gon ; Hong, Soon-Kwan ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 301~307
DOI : 10.5010/JPB.2011.38.4.301
The medicinal herb Oldenlandia diffusa is known as a folk medicine for the treatment of hepatitis, sore throat, appendicitis, malignant tumors and urethral infection in Southern China and Korea. Another species O. corymbosa, is also used for the therapy of the similar conditions, however, only O. diffusa is referred to the medicinal herb by Chinese Pharmacopoeia. Due to their similar morphology, O. diffusa and O. corymbosa are often misidentified. To easily identify O. diffusa from O. corymbosa, the phylogenetic utility of nuclear ribosomal DNA (nrDNA) internal transcribed spacers (ITS) were investigated among different O. diffusa and O. corymbosa populations in Korea. The nrDNA ITS sequence of O. diffusa contained 791 bp, with GenBank accession number of JF837601-JF837602. The nrDNA ITS sequence of O. corymbosa was 785-786 bp, with GenBank accession number of JF837603-JF837611. The results showed that there are some certain divergences in the ITS region sequence between both species, even among different populations of the same species. Particularly, O. corymbosa ST-4 population showed the highest dissimilarity of the ITS region sequence with other nine populations of O. corymbosa and two populations of O. diffusa. This consequence makes us further understand the molecular diversification between O. corymbosa and O. diffusa, and help to promote the correct use and safety.
GUS gene expression and plant regeneration via co-culturing with Agrobacterium in grapevine (Vitis vinifera)
Kim, Se-Hee ; Kim, Jeong-Hee ; Kim, Ki-Ok ; Do, Gyeong-Ran ; Shin, Il-Sheob ; Cho, Kang-Hee ; Hwang, Hae-Seong ;
Journal of Plant Biotechnology, volume 38, issue 4, 2011, Pages 308~314
DOI : 10.5010/JPB.2011.38.4.308
Efficient transformation and regeneration methods are a priority for successful application of genetic engineering to vegetative propagated plants such as grape. In this study, methods for Agrobacterium tumefaciens-mediated transformation and plant regeneration of grapevine (Vitis vinifera) were evaluated. Tamnara, Heukgoosul, Heukbosek, Rizamat were co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, GUS gene as reporter gene and resistance to kanamycin as selective agent. Seven percent of the maximum regeneration frequency was obtained from co-cultivated with explants from Rizamat with LBA4404 strain on selection medium with kanamycin. The addition of acetosyringone, 200
in virulence induction step was a key factor for successful GUS reporter gene expression in grapevine transformation. Transgenic plants showed resistance to kanamycin and the GUS positive response in leaf (
) stem (
) and petiole (