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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 39, Issue 4 - Dec 2012
Volume 39, Issue 3 - Sep 2012
Volume 39, Issue 2 - Jun 2012
Volume 39, Issue 1 - Mar 2012
Selecting the target year
Establishment of rapid discrimination system of leguminous plants at metabolic level using FT-IR spectroscopy with multivariate analysis
Song, Seung-Yeob ; Ha, Tae-Joung ; Jang, Ki-Chang ; Kim, In-Jung ; Kim, Suk-Weon ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 121~126
DOI : 10.5010/JPB.2012.39.3.121
To determine whether FT-IR spectroscopy combined with multivariate analysis for whole cell extracts can be used to discriminate major leguminous plant at metabolic level, seed extracts of six leguminous plants were subjected to Fourier transform infrared spectroscopy (FT-IR). FT-IR spectral data from seed extracts were analyzed by principal component analysis (PCA), partial least square discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA). The PCA could not fully discriminate six leguminous plants, however PLS-DA could successfully discriminate six leguminous plants. The hierarchical dendrogram based on PLS-DA separated the six leguminous plants into four branches. The first branch was consisted of all three Vigna species including Vigna radiata var. radiate, Vigna angularis var. angularis and Vigna unguiculata subsp. Unguiculata. Whereas Pisum sativum var. sativum, Glycine max L and Phaseolus vulgaris var. vulgaris were clustered into a separate branch respectively. The overall results showed that metabolic discrimination system were in accordance with known phylogenic taxonomy. Thus we suggested that the hierarchical dendrogram based on PLS-DA of FT-IR spectral data from seed extracts represented the most probable chemotaxonomical relationship between six leguminous plants.
Analysis of risk management system of GM crops in China for the development of global GM crops
Lee, Shin-Woo ; Cho, Kwang-Soo ; Wang, Zhi ; Kwak, Sang-Soo ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 127~132
DOI : 10.5010/JPB.2012.39.3.127
We analysed the current status of development of GM crops and national biosafety framework including legislation-related agricultural GMO in China to provide the policy for the development of global GM crops in Korea. In China, several GM crops including cotton, petunia, tomato, sweet pepper, poplar, and papaya have been approved for commercialization and they have been cultivated at more than 4 million ha. In addition, GM rice and GM maize have also obtained approval for productive testing in 2009. China will be the first country to approve GM rice for commercialization. Prior to commercialization in China, all GM crops must be approved by government authority for biosafety assessment specified by national legislation including restricted field testing, enlarged field testing, productive testing and safety certificate. According to China's legislation, agricultural GMOs have been classified by research and testing, production and processing. All GMOs must go through 3 steps of field testing (restricted, enlarged and productive). Prior to conducting each field testing, it has to be approved by government authority. It is assumed that at least one to two years will be taken for each step of field testing (total 4 to 8 years to obtain the final safety certificate) along with a large amount of budget.
The influence of silver thiosulfate and thidiazuron on shoot regeneration from cotyledon explants of Brassica napus
Roh, Kyung-Hee ; Kwak, Bo-Kyung ; Kim, Jong-Bum ; Lee, Kyeong-Ryeol ; Kim, Hyun-Uk ; Kim, Sun-Hee ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 133~139
DOI : 10.5010/JPB.2012.39.3.133
The influences of ethylene inhibitors (
and silver thiosulfate) and cytokinins (BAP and TDZ) on shoot regeneration from cotyledon and hypocotyl explants of B. napus cv. Youngsan were investigated. The presence of
Silver thiosulfate (STS) in shoot regeneration medium formed shoots at 60-68% after 3-4 weeks of culture, which was enhanced by 2-fold compared to that of Silver nitrate (
). Moreover, cotyledon explants were more regenerative than hypocotyls; shoots from cotyledon explants began to occur 4-5 days earlier than that of hypocotyl explants. TDZ at a concentration of
was effective for shoot regeneration, compared with BAP. Consequently, the optimal shoot regeneration response was observed in medium supplemented with
. In transmission electron microscopy (TEM) analysis, higher density of silver nanoparticles was shown to be accumulated widely inside the cell wall and plasmodesmata of regenerating leaf cultured in medium supplemented with
. By contrast, in the cell cultured in medium with STS, fine-grained deposits were partly observed in the surroundings of the cell wall.
FT-transgenic spray-type Chrysanthemum (Dendranthema grandiflorum Kitamura) showing early-flowering
Lee, Su-Young ; Han, Bong-Hee ; Hur, Eun-Joo ; Shin, Hak-Kee ; Lee, Il-Ha ; Lee, Eun-Kyung ; Kim, Seung-Tae ; Kim, Won-Hee ; Kwon, O-Hyeon ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 140~145
DOI : 10.5010/JPB.2012.39.3.140
The flowering locus T (FT) gene, of which expression will be controlled at high temperature by heat shock promoter (it printed as to HSproFT), was introduced into spray-type chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) 2 cultivars ('Pink PangPang' and 'Pink Pride' by co-cultivation with Agrobacterium tumefaciens strain C58C1 harboring pCAMBIA2300 containing the HSproFT gene. After leaf segments of the 2 cultivars were infected with the A. tumafaciens with C58C1 as explants, shoots were regenerated from the explants cultured on the
selection medium (MS basal salts + 1.0 mg/L BA, 0.5 mg/L IAA + 10 mg/L kanamycin + 0.7% plant agar, pH 5.8). The shoots were transferred into the
selection medium (MS basal salts + 1.0 mg/L BA, 0.5 mg/L IAA + 20 mg/L kanamycin + 0.7% plant agar, pH 5.8). One hundred seventeen plantlets from 'Pink PangPang' and 5 ones from 'Pink Pride' were confirmed as transformants by PCR analysis. Twenty six of the transformants and non-transformants were acclimatized and established well in a green house. Eights of 26 transgenic lines showed flower bud 1.7~10 days earlier than nontransgenic plants, and 24 of them flowered 1~6 days earlier than non- transgenic plants. The shape and color of flower of all HSproFT-transgenic lines were not different with those of non- transgenic plants.
Antioxidant property of leaves and calluses extracts of in-vitro grown 5 different Ocimum species
Song, Hyuk ; Kumar, Prem ; Arivazhagan, Girija ; Lee, Sang-Il ; Yoon, Hyung-Moon ; Kim, Ick-Hee ; Kwon, Hyuk-Jung ; Kim, Jong-Moon ; Hakkim, Faruck Lukmanul ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 146~153
DOI : 10.5010/JPB.2012.39.3.146
In this study, the antioxidant property of leaf and callus extracts of five selected in vitro grown Ocimum species (Ocimum sanctum, Ocimum kilimandscharicum, Ocimum gratissimum, Ocimum basilicum, and Ocimum americanum) and their respective callus extracts was investigated. The callus cultures were successfully initiated on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (1mg L) combined with different concentrations (0.1-0.4 mg L) of kinetin as plant growth regulators. Total phenolic contents were estimated using the Folin-Ciocalteu reagent. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power,
chelating activity, and
-carotenelinoleic acid bleaching assays were used to determine the biological effects of the extracts. Interestingly, all the callus extracts exhibited significant (p<0.05) increase in phenolic contents and antioxidant activity. Furthermore, a liner correlation was obtained between the total phenolic contents and free radical scavenging activity (
= 0.783). The extracts of leaves and calluses of Ocimum species exhibited activity in all the in vitro antioxidant assays, but its extent was less potent that the positive controls butylated hydroxyl anisole (BHA) and ascorbic acid. A higher accumulation of phenolics in the callus extracts suggests that isolation of high-concentration materials with antioxidant activivity is possible from in vitro callus cultures rather than field-grown plant organs. Furthermore, these extracts may be used as an effective preservative in the food industry.
Growth, quality, and yield characteristics of transgenic potato (Solanum tuberosum L.) overexpressing StMyb1R-1 under water deficit
Im, Ju-Sung ; Cho, Kwang-Soo ; Cho, Ji-Hong ; Park, Young-Eun ; Cheun, Chung-Gi ; Kim, Hyun-Jun ; Cho, Hyun-Mook ; Lee, Jong-Nam ; Jin, Yong-Ik ; Byun, Myung-Ok ; Kim, Dool-Yi ; Kim, Myeong-Jun ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 154~162
DOI : 10.5010/JPB.2012.39.3.154
This study was conducted to evaluate agronomic characteristics such as growth, quality, and yields of StMyb1R-1 transgenic potato and also to obtain the basic data for establishing assessment guidelines of transgenic potato. Three transgenic lines (Myb 1, Myb 2, and Myb 8) were cultivated under conventional irrigation, drought condition, and severe drought condition and were analyzed by comparing with wild type, non-transgenic cv. Superior. Myb 2 showed a different flower color from wild type and Myb 1 had much bigger secondary leaflets than wild type. Myb 1 and Myb 2 showed higher
content in both top and root zone and longer shaped tubers than wild type. In yield factors, transgenic lines had more tubers than wild type, however their yield decreases were severe because of the poor enlargement of tuber under water deficit condition. This tendency was noticeable in Myb 1 and Myb 2. In TR ratio, chlorophyll content, dry matter rate, and relative water content, there were no big differences between transgenic lines and wild type. Meanwhile, in phenotype, growth, quality, and yield factors, substantial equivalent was confirmed between Myb 8 and wild type. Then, Myb 8 showed the highest marketable tuber yield under conventional irrigation, while showed lower level than wild type under water deficit. Judged by this result, the enhancing droughttolerance by StMyb1R-1 gene might actually not mean the enhancement of photosynthesis or starch accumulation in tuber and, furthermore, not the yield improvement. More detailed research will be required to accurately understand the relationship between StMyb1R-1 and yield factors.
In vitro germination of Gastrodia verrucosa Blume and Hetaeria sikokiana Tuyama treated by NaOCl
Bae, Kee-Hwa ; Ko, Myoung-Suk ; Choi, Sun-A ; Lee, Hak-Bong ; Kim, Nam-Young ; Song, Jae-Mo ; Song, Gwan-Pil ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 163~168
DOI : 10.5010/JPB.2012.39.3.163
An optimization in vitro seed germination was established by using triphenol tetrazolium chloride (TTC) test, which has been known as two rare orchids (Gastrodia verrucosa Blume and Hetaeria sikokiana Tuyama) in Jeju island. We have established proper NaOCl treatment for in vitro germination of G. verrucosa and H. sikokiana through TTC test. In the case of H. sikokiana, seed viability through TTC test was high with 95% in control. However, NaOCl 1% treatment for 30 minutes showed the highest embryo swelling rate to seed viability. Likewise, swelling formation of embryos, diameter of embryos, protocorm formation and diameter of protocorms of G. verrucosa was 87%,
, 91% and
through NaOCl 1% treatment for 30 minutes. This result will be applied on the basic information for improving in vitro seed germination rate of G. verrucosa and H. sikokiana.
Quantitative trait loci (QTLs) detection for plant regeneration ability from seed culture in rice (Oryza sativa L.)
Liu, Meihan ; Sohn, Jae-Keun ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 169~174
DOI : 10.5010/JPB.2012.39.3.169
Quantitative trait loci (QTLs), which were related to the ability of callus induction and plant regeneration in seed culture of rice, were analyzed using a mapping population from a cross between the rice cultivars 'Samgang' (tongil type) and 'Nagdong' (japonica). A tongil type rice cultivar, 'Samgang' showed lower frequency (20%) of plant regeneration than that (35%) of japonica rice, 'Nagdong'. Transgressive segregations were observed for the ability of callus induction and plant regeneration from the seed-derived calli of 58 doubled haploid (DH) lines. The ability of plant regeneration of 58 doubled haploid lines showed a continuous distribution with comparatively wide range (10.0 to 66.7%) of variation. Composite interval mapping analysis was used to identify the QTLs controlling callus induction and plant regeneration ability. Four significant QTLs, qCWS6, qCWS8, qCWS9 and qCWS11, associated with callus weight per seed were detected on chromosomes 6, 8, 9, and 11 with LOD values of 3.30, 2.60, 2.70 and 2.43, explaining 36% of the total phenotypic variation. Three significant QTLs, qPR1, qPR6, and qPR11, for the ability of plant regeneration were located on chromosome 1, 6, and 11 at LOD score of 2.25, 2.15 and 2.55, accounting for 24 % of the total phenotypic variation. The present study should be useful for improving the efficiency of plant regeneration in tissue culture of indica rice by means of marker-assisted selection.
Molecular cloning and characterization of a soybean GmMBY184 induced by abiotic stresses
Chung, Eun-Sook ; Kim, Koung-Mee ; Lee, Jai-Heon ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 175~181
DOI : 10.5010/JPB.2012.39.3.175
Drought and high salinity stresses often imposes adverse effects on crop yield. MYB transcription factors have been shown to be an important regulator in defense responses to these environmental stresses. In this study, we have cloned and characterized a soybean gene GmMYB184 (Glycine max MYB transcription factor 184). Deduced amino acid sequences of GmMYB184 show highest homology with that from Vitis vinifera legume plant (75%). Different expression patterns of GmMYB184 mRNA were observed subjected to drought, cold, high salinity stress and abscisic acid treatment, suggesting its role in the signaling events in the osmotic stress-related defense response. Subcellular localization studies demonstrated that the GFP-GmMYB184 fusion protein was localized in the nucleus. Using the yeast assay system, the C-terminal region of GmMYB184 was found to be essential for the transactivation activity. These results indicate that the GmMYB184 may play a role in abiotic stress tolerance in plant.
Callus induction and plant regeneration of Iris dichotoma Pall. in endangered species
Bae, Kee-Hwa ; Yoo, Kyoung-Hwa ; Lee, Hak-Bong ; Yoon, Eui-Soo ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 182~188
DOI : 10.5010/JPB.2012.39.3.182
Iris dichotoma Pall. is an important endangered plant belonging to the family Iridaceae. A method was developed for the rapid micropropagation of I. dichotoma through plant regeneration from leaf, rhizome, and root explant-derived calli. Leaf, rhizome, and root segments were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D;
) for callus induction. Callus production was highest at
2,4-D, where 73.8% and 45.5% of cultured rhizome and root cuttings, respectively, produced calli. The viable calli were maintained at an induced concentration of 2,4-D (
). They were then transferred to MS medium supplemented with various concentrations of 2,4-D (
) in combination with 6-benzyladenine (BA: 0, 1.0 and
) for adventitious shoot regeneration. The addition of a low concentration of 2,4-D into BA-containing medium significantly increased the frequency of shoot regeneration in leaf, rhizome, and root-derived calli. The highest number of adventitious shoots (26.4 per callus) formed at
2,4-D and 1.0 mg/l BA. For rooting of the shoots, half- strength MS medium supplemented with different concentrations of indole 3-butyric acid (IBA)
was tested. The optimal results were observed using half-strength MS medium supplemented with
IBA, on which 98% of the regenerated shoots developed roots with an average of 3.5 roots per shoot within 45 days. The plantlets raised in vitro were acclimatized and transferred to soil with 95% success. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.
Molecular and functional characterization of a Brmecp gene encoding 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from Brassica rapa
Jung, Yu-Jin ; Choi, Jang-Sun ; Sun, Ju-Nam ; Nou, Ill-Sup ; Cho, Yong-Gu ; Kang, Kwon-Kyoo ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 189~195
DOI : 10.5010/JPB.2012.39.3.189
In plants, the fifth step of the plastidial 2-Cmethyl-D-erythritol 4-phosphate (MEP) pathway is catalyzed by 2-C-Methyl-D-erythritol 2,4-cyclodiphosphate synthase (MECP; EC: 4. 6. 1. 12), an enzyme proposed to play a key role in the regulation of isoprenoid biosynthesis. Here we report the isolation and functional characterization of a 823 bp Brassica rapa MECP (Brmecp) cDNA encoding a deduced polypeptide of 230 amino acid residues. Transcription levels of Brmecp were two-fold higher in petal compared to leaves. In addition, Brmecp expression in cabbage seedlings treated with ABA,
and drought was higher than control seedlings. These results were consistent with changes in chlorophyll contents in transgenic Arabidopsis. Thus, the Brmecp may contribute to the production of primary (chlorophylls and carotenoids) isoprenoid end-products in chloroplasts.
In vitro propagation of oil palm (Elaeis guineensis Jacq.) clones through somatic embryogenesis and analysis of somaclonal variation by RAPD
Ahn, In-Suk ; Park, Hye-Rim ; Son, Sung-Ho ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 196~204
DOI : 10.5010/JPB.2012.39.3.196
This study was carried out to develop reliable systems for somatic embryogenesis in oil palm tree (Elaeis guineensis Jacq.), and to verify the somaclonal variants by RAPD analysis. Embryogenic callus was induced successfully on modified half-strength MS medium containing
and casein. Embryogenic callus was further developed to somatic embryo mass (SEM), which is very hard and bonded tightly each other. Plantlets were proliferated when SEM was cultured on modified MS medium containing half strength
, casein and L-ascorbic acid. Plantlets were transplanted into pots containing artificial soils. When RAPD analysis was conducted using randomly selected 95 in vitro plantlets and 19 random primers, somaclonal variation was detected using BNR35 primer. There was missing band around 1 kb in #22, #28, #35, and #77 plantlets. In addition, bands obtained from #28, #35, and #77 was much stronger than other normal bands. The blast results at NCBI revealed that somaclonal variation observed in this study was related to chloroplast genome of oil palm. The results also revealed that oil palm reproduction system through somatic embryogenesis is quite reliable and early detection of somaclonal variants seem to be possible at in vitro stage by RAPD analysis.
In vitro induction of hairy root from isoflavones-producing Korean wild arrowroot Pueraria lobata
Kim, Soo-Jung ; Cha, Min-Seok ; Lee, Eun-Ji ; Kim, In-Hye ; Kwon, Jung-Eun ; Kang, Se-Chan ; Park, Tae-Ho ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 205~211
DOI : 10.5010/JPB.2012.39.3.205
Pueraria lobata is a perennial legume plant, widely distributed in the countries of East Asia. It is a medicinally important leguminous plant and produces various isoflavones such as puerarin, daidzein etc which have potential for preventing several chronic diseases including osteoporosis, cardiovascular disease and cancer. In this study, we tried to induce hairy roots in vitro from Korean wild arrowroot P. lobata and investigated the effects of hormones and light conditions. Initially leaf and stem segments were infected with Agrobacterium rhizogenes and incubated in different conditions. Hairy roots were induced from only stem segments and the induction was best at dark condition and the presence of IBA during incubation. Secondary roots were also significantly much more induced at the dark condition than at the 16 hours light condition. Among plant growth regulators of auxin, IBA was best for secondary root formation while 2,4-D, IAA and NAA produced callus or less hairy roots. The presence of the foreign gene rolC transferred by A. rhizogenes that plays a major role in hairy root induction was confirmed by PCR. The accumulation of isoflavones such as puerarin and daidzin was also confirmed. These results will facilitate mass production of hairy root and can be used for the production of functional substances from wild arrowroots.
Isolation and functional characterization of BrUGT gene encoding a UDP-glycosyltransferase from Chinese cabbage (Brassica rapa)
Jung, Yu-Jin ; Lee, Hye-Jung ; Choi, Jang-Sun ; Cho, Yong-Gu ; Nou, Ill-Sup ; Kang, Kwon-Kyoo ;
Journal of Plant Biotechnology, volume 39, issue 3, 2012, Pages 212~218
DOI : 10.5010/JPB.2012.39.3.212
Glycosyltransferases are enzymes (EC 2.4) that catalyze the transfer of monosaccharide moieties from activated nucleotide sugar to a glycosyl acceptor molecule which can be a carbohydrate, glycoside, oligosaccharide, or a polysaccharide. In this study, a UDP-glucosyltransferase cDNA was isolated from Brassica rapa using a rapid amplification of cDNA ends (RACE) and subsequently named BrUGT. It has a full-length cDNA of 1,236 bp with 119 bp 5'-untranslated region (UTR), a complete ORF of 834 bp encoding a polypeptide of 277 amino acids (31.19 kDa) and a 3'-UTR of 283 bp. BLASTX analysis hits a catalytic domain of Glycos_transf_1 super family (cl12012) that belongs to the Glycosyltransferases group 1 with tetratricopeptide (TPR) regions located between 165 to 350 bp. Expression analysis showed high mRNA transcripts in pistil, followed by petal, seed and calyx of flower. Moreover, expression analysis of BrUGT in Chinese cabbage seedlings under stresses of cold, salt, PEG,
, drought and ABA showed elevated mRNA transcript. Furthermore, when BrUGT gene was transformed into rice using pUbi-1 promoter, overexpression was evident among the
plants. This study provides insights into the function of BrUGT in plants.