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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
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Volume & Issues
Volume 39, Issue 4 - Dec 2012
Volume 39, Issue 3 - Sep 2012
Volume 39, Issue 2 - Jun 2012
Volume 39, Issue 1 - Mar 2012
Selecting the target year
Alstroemeria plants and its biotechnological applications
Lim, Sung-Soo ; Lee, Sang-Il ; Kang, Se-Chan ; Kim, Jong-Bo ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 219~224
DOI : 10.5010/JPB.2012.39.4.219
Alstroemeria plants are widely cultivated in many countries especially in Western Europe and North America and popularity has increased in recently due to its long-base life, large variety of colors and low energy requirement during cultivation period. So far, more than 60 species have been released on the commercial market in the world. To meet the demand of consumer and develop the elite Alstroemeria cultivars, conventional breeding including cross-hybridization and selection as well as mutation breeding were used. However, as other important ornamental plants such as lily, rose, carnation and orchids accepted the biotechnological methods, this newly-born approach should be applied and developed an optimized the genetic transformation system. Then, this biotechnological approach can be fused with the conventional breeding methods and thus can be contributed to the production of elite Alstroemeria plants containing agriculturally good genetic traits which are useful for the both farmers and consumers in the future. In this paper, we reviewed the botanical and genetical features of Alstroemeria plants and its biotechnological approaches in the last decades.
Recent trends in tissue culture and genetic transformation of Phalaenopsis
Roh, Hee-Sun ; Lee, Sang-Il ; Lee, Yi-Re ; Baek, Sun-Young ; Kim, Jong-Bo ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 225~234
DOI : 10.5010/JPB.2012.39.4.225
This report describes recent advances in tissue culture and genetic transformation of commercial Phalaenopsis. Recently, an importance of Phalaenopsis has been increased due to its popularity with beautiful flowers and is widely used for pot plants as well as cut-flower. Its use is rapidly enlarging in worldwide. Thus, demands for the release of new elite cultivars in Phalaenopsis have been increased. During the last several decades, some critical progresses have been made in tissue culture and genetic transformation in Phalaenopsis species. Cooperation with these biotechnological methods are supposed to promote the release of commercial Phalaenopsis cultivars in the near future. Until now, no technical review on tissue culture and genetic transformation in Phalaenopsis has been reported in Korea. Therefore, we inquired the brief history and techniques of tissue culture system in Korea.
Characterization of small ubiquitin-like modifier E3 ligase, OsSIZ1, mutant in rice
Park, Hyeong Cheol ; Koo, Sung Cheol ; Kim, Hun ; Choi, Wonkyun ; Yun, Dae-Jin ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 235~241
DOI : 10.5010/JPB.2012.39.4.235
Sumoylation is a reversible conjugation process that attaches the small ubiquitin modifier (SUMO) peptide to target proteins and regulates a wide variety of cellular functions in eucaryotes. As final step of the sumoylation, SUMO E3 ligases facilitate conjugation of SUMO to target proteins. To characterize the functions of the SUMO E3 ligases in Oryza sativa, we isolated a single recessive rice SUMO E3 ligase, Ossiz1-2 mutant. In addition, we also confirmed the interaction between OsSIZ1/-2 and OsSUMO1, respectively, by using an Agrobacterium-based tobacco luciferase transient expression system. Ossiz1-2 mutant exhibited approximately 20% reduction in growth and developmental units compared with wild type. Especially, number of filled seeds and total seed weight were dramatically decreased in the Ossiz1-2 mutant rice. Thus, these results suggest that sumoylation by the OsSIZ1 as SUMO E3 ligase plays an important role in regulating growth and development in rice.
Efficiency for increasing seed oil content using WRINKLED1 and DGAT1 under the control of two seed-specific promoters, FAE1 and Napin
Kim, Hyojin ; Kim, Hyun Uk ; Suh, Mi Chung ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 242~252
DOI : 10.5010/JPB.2012.39.4.242
Seed storage oils are essential resources for not only human and animal diets but also industrial applications. The primary goal of this study was to increase seed oil content through comparative analysis of two seed-specific promoters, AtFAE1 from Arabidopsis Fatty Acid Elongase 1 gene and BnNapin from Brassica napus seed storage protein gene. AtWRI1 and AtDGAT1 genes encoding an AP2-type transcription factor and a Diacylglycerol Acyltransferase 1 enzyme, respectively, were expressed under the control of AtFAE1 and BnNapin promoters in Arabidopsis. The total seed oil content in all transgenic plants was increased by 8-11% compared with wild-type seeds. The increased level of oil content in AtWRI1 and AtDGAT1 transgenic lines under the control of both promoters was similar, although the activity of the BnNapin promoter is much stronger than that of AtFAE1 promoter in the mature stage of developing seeds where storage oil biosynthesis occurs at a maximum rate. This result demonstrates that the AtFAE1 promoter as well as the BnNapin promoter can be used to increase the seed oil content in transgenic plants.
Increased biomass and enhanced tolerance to salt stress in Chinese cabbage overexpressing Arabidopsis H
Park, Mehea ; Won, Hee-Yeun ; Kim, Chang Kil ; Han, Jeung-Sul ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 253~260
DOI : 10.5010/JPB.2012.39.4.253
On the basis of the reported agriculturally valuable phenotypes resulted from ectopic overexpression of Arabidopsis vacuolar
-PPase (AVP1), we generated the Chinese cabbage lines expressing AVP1 which then subjected to salt stress to determine the AVP1 expression if it consistently confers the capability for increasing biomass and enhancing tolerance to salinity in other species. Collectively, here we demonstrate that the transgenic young plants show more vigorous growth and higher tolerance to salt stress than wild-type ones. Increased biomass phenotype by AVP1 expression was supported by comparing fresh and dry weights of transgenic and wild type plants grown under normal condition, while higher salt tolerance trait was confirmed by tracing the kinetics of photosystem II quantum yield and DAB-staining under gradually intensified salt stress induced by MS salt or NaCl, followed by normal condition.
Comparative proteomic analysis of plant responses to sound waves in Arabidopsis
Kwon, Young Sang ; Jeong, Mi-Jeong ; Cha, Jaeyul ; Jeong, Sung Woo ; Park, Soo-Chul ; Shin, Sung Chul ; Chung, Woo Sik ; Bae, Hanhong ; Bae, Dong-Won ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 261~272
DOI : 10.5010/JPB.2012.39.4.261
Environmental factors greatly influence the growth, development, and even genetic characteristics of plants. The mechanisms by which sound influences plant growth, however, remain obscure. Previously, our group reported that several genes were differentially regulated by specific frequenciesof sound treatmentusing a sound-treated subtractive library. In this study, we used a proteomic approach to investigate plant responses to sound waves in Arabidopsis. The plants were exposed to 250-Hz or 500-Hz sound waves, and total proteins were extracted from leaves 8 h and 24 h after treatment. Proteins extracted from leaves were subjected to 2-DE analysis. Thirty-eight spots were found to be differentially regulated in response to sound waves and were identified using MALDI-TOF MS and MALDI-TOF/TOF MS. The functions of the identified proteins were classified into photosynthesis, stress and defense, nitrogen metabolism, and carbohydrate metabolism. To the best of our knowledge, this is the first report on the analysis of protein changes in response to sound waves in Arabidopsis leaves. These findings provide a better understanding of the molecular basis of responses to sound waves in Arabidopsis.
Comparison of transcriptome analysis between red flash peach cultivar and white flash peach cultivar using next generation sequencing
Kim, Se Hee ; Nam, Eun Young ; Cho, Kang-Hee ; Shin, Il Sheob ; Kim, Hyun Ran ; Hwang, Hae Seong ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 273~280
DOI : 10.5010/JPB.2012.39.4.273
Differences of gene expression between red flash peach cultivar and white flash peach cultivar were investigated by Nest-generation sequencing (NGS). EST from the red flash peach cultivar and white flash peach cultivar were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, temperature stress, ethylene signal pathway were significantly higher in white flash peach cultivar than in red flash peach cultivar. On the other hand, the up-regulation of proteins involved in anthocyanin and flavonol biosynthesis and protein degradation and sorbitol metabolism were observed in red flash peach cultivar. Chalcone synthase was preferentially expressed in the red flesh peach cultivar, agreeing with the accumulation of anthocyanin and expression of other previously identified genes for anthocyanin biosynthesis. Anthocyanin pathway related genes CHS, F3H, DFR, LDOX, UFGT differentially expressed between red flash peach cultivar and white flash peach cultivar. These results suggest that red flash peach cultivar and white flash peach cultivar have different anthocyanin biosynthesis regulatory mechanisms.
In vitro culture of rare plant Bletilla striata using Jeju magma seawater
Bae, Kee-Hwa ; Kim, Ki Ju ; Kim, Nam Young ; Song, Jae Mo ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 281~287
DOI : 10.5010/JPB.2012.39.4.281
This experiment was conducted to investigate the effect of various type of Magma seawater (MSW) concentrations on plant growth and useful mineral contents in Bletilla striata. In the RO (Reverse Osmosis) and ED (Electronic Distal) treatment, hardness of medium was poored in 3.0 g/L gelrite but increased in 8.0 g/L plant agar, 38,000 and
respectably. We analyzed the morphological and physiological characteristics differences of B. striata treated various MSW. Survival frequency of plant and growth (shoot length, shoot diameter, root length, root diameter, shoot/root ratio) were significantly increased in RO and ED treatment at 50% and 10%, especially. Chlorophyll contents in ED treatments were higher than those in control and RO treatment. The content of strontium (Sr) in 20, 50, 75, 100% ED treatment, were higher than those in the control and RO, ED 1, 5, 10% treatment. These results showed that treatment of ED with the range of 20~100% could be used to supply the strontium enriched orchid plant. It is considered that MSW may be applied for use in Magma seawater to promotion of growth and produced functional plant.
In vitro shoot regeneration from leaf tissue of "Whangkeumbae" pear(Pyrus pyrifolia Nakai)
Chun, Jae An ; Do, Kyung Ran ; Kim, Se Hee ; Cho, Kang-Hee ; Kim, Hyun Ran ; Hwang, Hae Sung ; Shin, Il Sheob ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 288~294
DOI : 10.5010/JPB.2012.39.4.288
In order to establish an efficient adventitious shoot regeneration conditions from leaf explants for Asian pear `Whangkeumbae`, the effect of concentration and kinds of plant growth regulator and carbon source was investigated. Leaf explants of cultures grown on Murashige and Skoog (MS) medium containing 8 g/L plant agar were used. When the medium contained 0.25 mg/L thidiazuron (TDZ) and 0.3 mg/L indolebutyric acid (IBA), the adventitious shoot regeneration rate (ASRR) was greater as 61.1% than others treated and higher TDZ concentrations (2.5 and 5 mg/L) treatment significantly reduced the ASRR. As the effect of IBA and indoleacetic acid (IAA) concentration on the ASRR, 0.5 mg/L TDZ plus different concentration of IAA exhibited relatively high ASRR and 0.5 mg/L TDZ plus 0.3 mg/L IAA showed the highest ASRR of 76.7%. Also the effect of sucrose and sorbitol as carbon source on regeneration was examined. The highest ASRR and the most shoots per explants averaged 94.4% and 3.49 by treatment of 30 mg/L sorbitol, respectably. Sorbitol is considered better carbon source than sucrose for shoot regeneration of `Whangkeumbae` pear.
Agronomic characteristics and field resistance to bacterial soft rot of transgenic potato overexpressing the soybean calmodulin 4 gene (SCaM4)
Sohn, Hwangbae ; Cho, Kwangsoo ; Cho, Jihong ; Gwon, Ohgeun ; Cheon, Chunggi ; Choi, Jigyeong ; Chung, Woosik ; Lee, Shin Woo ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 295~299
DOI : 10.5010/JPB.2012.39.4.295
We performed in vitro assay and field trials to assess levels of changes in intrinsic properties and resistance against soft rot of the potato cv. Dejima upon the introduction of a soybean calmodulin 4 gene (SCaM4). Field trials with four lines overexpressing SCaM4 gene were conducted over two seasons, and harvested tubers were evaluated in bioassay for resistance to Pectobacterium carotovorum ssp. carotovorum. The SCaM4 transgenic potato lines inoculated with
CFU/ml of P. carotovorum ssp. carotovorum showed enhanced resistance compared to control. Among the SCaM4 transgenic lines, the transgenic line SCaM4-4 exhibited the highest tolerance to soft rot in vitro assays, so did in field trials. In the field trial, the soft rot resistance of SCaM4-4 line was more than 5 times higher compared to that of control cultivar, Dejima. The major agronomic characteristics of the SCaM4 transgenic lines were not different from those of the nontransgenic `Dejima`. The result demonstrated that the transformation of a calmodulin 4 gene was a successful strategy in development of potato cultivar enhanced to soft rot.
Changes of asparagine content in potato (Solanum tuberosum L.) tuber during storage
Jin, Yong Ik ; Cho, Ji Hong ; Chang, Dong Chil ; Im, Ju Sung ; Park, Young Eun ; Yu, Hong Sub ; Jeong, Jin Cheol ; Park, Kyeong Hun ; Chung, Il Min ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 300~304
DOI : 10.5010/JPB.2012.39.4.300
In order to research changes of asparagine content according to stage after harvest to storage, it was analysed asparagine content of potato tuber and potato sprout. The results of the asparagine content in potato cultivars after harvest ranked in cv. Superior, cv. Gahwang, cv. Atlantic and cv. Goun in descending order. The content of asparagine in potato was approximately 1300 mg/100g (D.W.) The changes the asparagine content according to storage condition and the difference of asparagine between tuber and sprout can be summarized as follows. In the asparagine content of potato cultivars, Little variation in the content was observed while potato tubers were stored at
. However, after 3 months of storage at
, the content increased by 9.7%, indicating that the content increases as the storage temperature and period increases. The content of asparagine also increased during sprouting of potato tubers. In the sprout, the proximal region contained 21% higher content of asparagines than the distal region, which suggests that the sprout develops with the increasing of the content of asparagines.
Promotion of in vitro shoot proliferation in rose by addition of liquid medium to culture
Lee, Ye Ji ; Lee, Jung Lim ; Hyung, Nam-In ; Kim, Seung Tae ; Lee, Eun Kyung ; Kwon, O Hyeon ; Kim, Won Hee ; Lee, Su Young ;
Journal of Plant Biotechnology, volume 39, issue 4, 2012, Pages 305~308
DOI : 10.5010/JPB.2012.39.4.305
To promote the growth and proliferation of in vitro rose (Rosa hybrida L) shoots, a liquid medium was added to shoot culture. Shoots were obtained by culturing internodes of four cultivars, `Antique Curl`, `Shiny Orange`, `White Zen`, and `Red Zen`, and then were proliferated by the subculture two times. An addition with 10~15 mL of liquid medium enhanced the shoot elongation of all four cultivars. However, the effect of liquid medium addition to culture of in vitro shoot for proliferation was dependent on cultivars of rose.