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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 40, Issue 4 - Dec 2013
Volume 40, Issue 3 - Sep 2013
Volume 40, Issue 2 - Jun 2013
Volume 40, Issue 1 - Mar 2013
Selecting the target year
Effect of Irradiation on the Biological System in Plants
Choi, Jong-Il ; Kim, Jin-Kyun ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 111~124
DOI : 10.5010/JPB.2013.40.3.111
Research on the basic interaction of radiation with biological systems has contributed to human society through various applications in pharmaceutical, medicine, agriculture and other technological developments. In the agricultural sciences and food technology sectors, the last few decades have witnessed a large number of pertinent works regarding the utilization of radiation for evolution of superior varieties of agricultural crops of economic importance. This review presents general information about the effect of radiation on plant specificity, dose response, and benefits. There has been summarized of the effects observed after exposure and influenced by several factors including plant characteristics and radiation features. We also report on the effect of
-irradiations on plants, focusing on metabolic alterations, modifications of growth and development and changes in biochemical pathways.
Plastoglobule in chloroplast and its role in prenylquinone metabolism
Kim, Hyun Uk ; Kim, Eun-Ha ; Lee, Kyeong-Ryeol ; Jung, Su-Jin ; Roh, Kyung Hee ; Kim, Jong-Bum ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 125~134
DOI : 10.5010/JPB.2013.40.3.125
Lipid droplets called plastoglobules are present in all plastid types. In chloroplasts, they are surrounded by the outer lipid monolayer from and connected to thylakoid membrane. The plastoglobule core contains the neutral lipids, which includes prenylquinones, triacylglycerols, and carotenoids. During stress and various developmental stages such as senescence, the size and number of plastoglobules increase due to the accumulation of lipids. Plastoglobules proteome revealed the presence of metabolic enzymes as well as structural proteins, plastoglobulins/fibrillins. Among the metabolic enzymes, the tocopherol cyclase, VTE1 and the NADPH quinine dehydrogenase, NDC1 have demonstrated that these participate in isoprenoid lipid metabolic pathways at the plastoglobule, notably in the metabolism of prenylquinones (tocopherol, plastoquinol and phylloquinone).
Transgene structures of marker-free transgenic Bt rice plants
Woo, Hee-Jong ; Lee, Seung Bum ; Lim, Myung-Ho ; Gwon, Sun-Jong ; Lee, Jin-Hyoung ; Shin, Kong-Sik ; Cho, Hyun-Suk ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 135~140
DOI : 10.5010/JPB.2013.40.3.135
A less simple approach developed for generation of marker-free transgenic plants is to select transformants without the use of selective marker genes. Some results about development of marker-free transgenic plants were obtained using a non-selective approach in several crops such as rice, potato and tobacco. However, the study did not provide evidence on detailed characterization of introduced gene on genome, a critical step for confirming the stable integration and transmission of a foreign gene. In this study, we evaluated structure and integration sites of transgene (mCry1Ac) in the transgenic Bt rice plants which were made via conventional Agrobacterium-mediated transformation by non-selective method. Structure and integration sites of transgene in these transgenic plants had similar fashion as those recovered under selection.
Effect of kinds and concentrations of osmoticum on somatic embryo induction and germination from suspended embryogenic cell in Larix kaempferi
Kim, Yong-Wook ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 141~146
DOI : 10.5010/JPB.2013.40.3.141
This study was conducted to examine suspended embryogenic cells growth with days of culture, effects of various kinds/concentrations of osmoticum for induction of somatic embryos (SEs), following somatic embryos germination or plantlet regeneration. The proliferation pattern of embryogenic cells in suspension culture is characterized by settled cells volume (SCV) increased with the duration of culture with marked the maxium of SCV (10.1 ml) in 18 days of culture, however the SCV of cells gradually decreased after that. In comparison of kinds/concentrations of osmoticum on somatic embryo induction, the highest induction number (352.3/g FW) of the SE was showed in 0.2 M sucrose, in addition, we also observed some effects with treatments of 0.2 M maltose (203.7) and 0.3 M maltose (193.7), respectively. However, no somatic embryos produced in treatments of 7.5% PEG plus 0.15 M sucrose or maltose. In comparison of germination efficiency of SEs which occurred from the treatments of various kinds/ concentrations of osmoticum, the highest induction frequency of cotyledon (25.2%) was obtained from SEs that produced 0.3 M maltose, however, the best occurrence rates of hypocotyl (39%), radicle (30.3%) and plantlet regeneration (3.5%) were observed from the 0.2 M sucrose treatment, respectively.
Microtuber Formation from In Vitro Codonopsis lanceolata Plantlets by Sugar
Kim, Ji-Ah ; Moon, Heung-Kyu ; Choi, Yong-Eui ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 147~155
DOI : 10.5010/JPB.2013.40.3.147
In this experiment, we report for the first time mass propagation by in vitro mircrotuberization of Codonopsis lanceolata. We first examined the effect of cytokinins on multiple shoot induction. 2.0
of kinetin not only gave the highest rate of shoot induction (19.1%) but also the elongation of shoot (17.1 mm). Secondly, we investigated the effect of sugars on in vitro microtuberization from nodal segments. The diameter of tuberous roots was enlarged in the half-strength MS medium supplemented with 145.9 mM sucrose. Histological analysis revealed that the number of parenchymatous cell containing starch grains increased in the tuberous roots. In addition, unlike in non-tuberous root, vascular bundles were scattered inner cortex layer. Thirdly, in order to preserve and stimulate the germination, microtubers were stored at
refrigerator during 9 months and then transplanted to the artificial soils (vermiculrite : peatmoss = 1:1 v/v), resulting that the rates of survival and germination were 75% and 70%, respectively. These results indicated that mass propagation of C. lanceolata was achieved by in vitro microtuber formation, suggesting that this protocol might be applied for not only the propagation of elite clones but also conservation of C. lanceolata germplasm.
Physiological responses to drought stress of transgenic Chinese cabbage expressing Arabidopsis H+-pyrophosphatase
Jeong, Mihye ; Kang, In-Kyu ; Kim, Chang Kil ; Park, Kyung Il ; Choi, Cheol ; Han, Jeung-Sul ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 156~162
DOI : 10.5010/JPB.2013.40.3.156
Plant tolerance to drought is a beneficial trait for stabilizing crop productivity under water deficits. Here we report that genetically engineered Chinese cabbage expressing Arabidopsis
-pyrophosphatase (AVP1) shows enhanced physiological parameters related to drought tolerance. In comparison with wild type plants under soil water deficit stress created by cessation of irrigation, soil water potential in pot with AVP1-expressing plants was more rapidly decreased that might lead to increased relative water content in leaves, while both genotypes had indistinguishable wilting phenotypes. Transgenic plants subjected to drought treatment also exhibited higher photosystem II quantum yield in addition to lower electrolyte leakage and
-diaminobenzidine content when compared to wild type plants.
Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis
Bae, Kee-Hwa ; Yoo, Kyoung-Hwa ; Lee, Mi-Hyun ; Jeong, Jae-Hun ; Choi, Yong-Eui ; Yoon, Eui-Soo ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 163~168
DOI : 10.5010/JPB.2013.40.3.163
Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.
Biosynthesis of recombinant human prominiinsulin in E. coli and plant systems
Choi, Yu Jin ; Park, Su Hyun ; Kim, Ji Su ; Wi, Soo Jin ; Park, Ky Young ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 169~177
DOI : 10.5010/JPB.2013.40.3.169
Recently, the number of people with diabetes is rapidly increasing, coupled with the fact that the insulin market is remarkably increasing. Therefore, molecular farming for plant-derived pharmaceutical protein production is reported as becoming more attractive than ever. In this study, we carried out experiments step by step for development of recombinant insulin constructs, which were transformed into E. coli system, in vitro transcription and translation system, and tobacco cells. At first, recombinant proinsulin protein was successfully produced in in vitro transcription and translation system with wheat germ extract. After which, recombinant construct of prominiinsulin encoded a fusion protein of 7.8 kDa with trypsin cleavage sites at N terminus and C terminus of minimized C-peptide was tried to in vitro expression using E.coli culture. After purification with His-tag column, the resulting recombinant prominiinsulin protein was processed with trypsin, and then checked insulin biosynthesis by SDS-PAGE and western blot analysis with anti-insulin monoclonal antibody. The immunoreactive product of trypsin-treated miniinsulin was identical to the predicted insulin hexamer. The construct of 35S promoter-driven preprominiinsulin recombinant gene with signal peptide region for ER-targeting and red fluorescence protein gene [N terminus
tobacco E2 signal peptide
B-peptide (1-29 AA)
A-peptide (1-21 AA)
C terminus] was transformed into BY-2 tobacco cells. A polypeptide corresponding to the 38-kDa molecular mass predicted for fusion protein was detected in total protein profiles from transgenic BY-2 cells by western analysis. Therefore, this recombinant preprominiinsulin construct can be used for generation of transgenic tobacco plants producing therapeutic recombinant insulin.
Increasement of antioxidative activity in Codonopsis lanceolata adventitious root treated by Methyl jasmonate and salicylic acid
Hwang, Hyun-Jung ; Song, Gwanpill ; Kim, Mi-Hyang ; Do, Seon-Gil ; Bae, Kee-Hwa ;
Journal of Plant Biotechnology, volume 40, issue 3, 2013, Pages 178~183
DOI : 10.5010/JPB.2013.40.3.178
Traditionally, Codonopsis lanceolata root have been used as a source of natural heath food. This study was initiated to investigate the impacts of methyl jasmonate (MeJA) and salicylic acid (SA) on adventitious growth C. lanceolata, the production of secondary metabolites, such as flavonoids, total phenolic compound, antioxidative activity (DPPH). The highest phenolics content was observed in treatment of 20 uM MeJA (74.53 mg/g). The content of total flavonoids followed the similar pattern as that of total phenolics, showing 38.45 mg/g of C. lanceolata treated by 20 uM MeJA. The DPPH scavenging activity was 24.2 (
) of C. lanceolata treated by 20 uM MeJA. These results provide useful information for enhancing biological properties of cultural roots of C. lanceolata.