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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 40, Issue 4 - Dec 2013
Volume 40, Issue 3 - Sep 2013
Volume 40, Issue 2 - Jun 2013
Volume 40, Issue 1 - Mar 2013
Selecting the target year
Recent advances in tissue culture and genetic transformation system of switchgrass as biomass crop
Lee, Sang Il ; Lim, Sung-Soo ; Roh, Hee Sun ; Kim, Jong Bo ;
Journal of Plant Biotechnology, volume 40, issue 4, 2013, Pages 185~191
DOI : 10.5010/JPB.2013.40.4.185
Over the past decades, carbon dioxide concentration of the atmosphere of the world has increased significantly, and thereby the greenhouse effect has become a social issue. To solve this problem, new renewable energy sources including solar, hydrogen, geothermal, wind and bio-energy are suggested as alternatives. Among these new energy sources, bio-energy crops are widely introduced and under rapid progress. For example, corn and oilseed rape plants are used for the production of bio-ethanol and bio-diesel, respectively. However, grain prices has increased severely because of the use of corn for bio-ethanol production. Therefore, non-edible switchgrass draws attention as an alternative source for bio-ethanol production in USA. This review describes the shortage of fossil energy and an importance of switchgrass as a bio-energy crop. Also, some characteristics of its major cultivars are introduced including growth habit, total output of biomass yields. Furthermore, biotechnological approaches have been conducted to improve the productivity of switchgrass using tissue culture and genetic transformation.
Phenotypic and molecular characteristics of second clone (T
) plants of the LeLs-antisense gene-transgenic chrysanthemum line exhibiting non-branching
Lee, Su Young ; Kim, Jeong-Ho ; Cheon, Kyeong-Seong ; Lee, Eun Kyung ; Kim, Won Hee ; Kwon, O Hyeon ; Lee, Hye Jin ;
Journal of Plant Biotechnology, volume 40, issue 4, 2013, Pages 192~197
DOI : 10.5010/JPB.2013.40.4.192
This study examined the phenotypic and molecular characteristics of the
) plants of LeLs-antisense gene-transgenic chrysanthemum line (LeLs80) that exhibited non-branching, proving the relevance of these characteristics as a factor for use in environmental risk assessment. Results of the Southern blot analysis showed that three copies of the LeLs-antisense gene were introduced into the transgenic line, and northern analysis showed that the transcripted gene was normally expressed in the transgenic line. A flanking T-DNA sequencing method was used to determine that sequences of 184 and 464 bps flanked the LeLs-antisense gene in the transgenic line. These sequences, respectively, matched the 35S promoter for expression of the npt II gene and the NOS terminator for expression of the LeLs-antisense gene within the pCAMBIA 2300 vector.
Development of transgenic cucumbers expressing Arabidopsis Nit gene
Jang, Hyun A ; Lim, Ka Min ; Kim, Hyun A ; Park, Yeon-Il ; Kwon, Suk Yoon ; Choi, Pil Son ;
Journal of Plant Biotechnology, volume 40, issue 4, 2013, Pages 198~202
DOI : 10.5010/JPB.2013.40.4.198
To produce transgenic cucumber expressing Nit gene coffering abiotic resistance, the cotyledonary-node explants of cucumber (cv. Eunsung) were inoculated with A. tumefaciens transformed with pPZP211 or pCAMBIA2300 carrying Nit gene, that has cis-acting element involved in resistance to various abiotic environmental stresses. After co-cultivation, the procedures of selection, shoot initiation, shoot elongation, and plant regeneration were followed by cotyledonary-node transformation method (CTM, Jang et al. 2011). The putative transgenic plants were selected when shoots were grown to a length greater than 3 cm from the cotyledonary-node explants on selection medium supplemented with 100 mg/L paromomycin as a selectable agent. The confirmation of transgenic cucumber was based on the genomic PCR, Southern blot analysis, RT-PCR, and Northern blot analysis. A 105 shoots (4.12%) selected from the selection mediums were obtained from 2,547 explants inoculated. Of them, putative transgenic plants were only confirmed with 45 plants (1.77%) by genomic PCR analysis. Transgenic plants showed that the Nit genes integrated into each genome of 39 plants (1.53%) by Southern blot analysis, and the expression of gene integrated into cucumber genome was only confirmed at 6 plants (0.24%) by RT-PCR and Northern blot analysis. These results lead us to speculate that the genes were successfully integrated and expressed in each genome of transgenic cucumber.
Shoot regeneration via culture of leaf explants in pear (Pyrus pyrifolia cv. Niitaka)
Kim, Se Hee ; Shin, Il Sheob ; Cho, Kang Hee ; Kim, Dae Hyun ; Kim, Hyun Ran ; Kim, Ki Ok ; Lee, Hyang Bun ; Do, Kyung Ran ; Chun, Jae An ; Hwang, Hae Seong ;
Journal of Plant Biotechnology, volume 40, issue 4, 2013, Pages 203~209
DOI : 10.5010/JPB.2013.40.4.203
Genetic manipulation of pear (Pyrus pyrifolia Nakai) breeding is still difficult due to lack of reliable regeneration system. The aim of this research is to establish shoot regeneration system from leaf explants for pear (P. pyrifolia cv. Niitaka) using various concentrations of plant growth regulators and carbon source supplemented to medium. The highest regeneration rate of about 20% was found on a medium containing 4.4 g/L of Murashige and Skoog (MS) without vitamins, Linsmaier and Skoog (LS) vitamins were added separately. Leaf explants of pear were cultured on MS medium containing 7 g/L of Daishin agar supplemented with various concentrations of NAA (0.01, 0.05, 0.1, 0.5 mg/L) in combination with BA(3, 5, 10 mg/L) for shoot regeneration. In medium with 5 mg/L of BA and 0.01 mg/L of NAA, adventitious shoot regeneration rate was higher than others treated. The optimal results were observed using MS medium supplemented with 30 g/L sorbitol as carbon source on regeneration system. Sorbitol is considered better carbon source than sucrose for shoot regeneration of pear (P. pyrifolia cv. Niitaka). In order to increase of shoot regeneration in pear (P. pyrifolia cv. Niitaka), plant agar and Daishin agar used as gelling agents, Daishin agar is more efficient in shoot regeneration.
Plant regeneration and transformation of grape (Vitis labrusca L.) via direct regeneration method
Kim, Se Hee ; Shin, Il Sheob ; Cho, Kang Hee ; Kim, Dae Hyun ; Kim, Hyun Ran ; Kim, Jeong Hee ; Lim, Sun-Hyung ; Kim, Ki Ok ; Lee, Hyang Bun ; Do, Kyung Ran ; Hwang, Hae Seong ;
Journal of Plant Biotechnology, volume 40, issue 4, 2013, Pages 210~216
DOI : 10.5010/JPB.2013.40.4.210
Efficient regeneration methods and transformation system are a priority for successful application of genetic engineering to vegetative propagated plants such as grape (Vitis labrusca L.). This research is to establish shoot regeneration system from plant explants for 'Campbell Early', 'Tamnara', 'Heukgoosul', 'Heukbosek' using two types of plant growth regulators supplemented to medium. The highest adventitious shoot regeneration rate of 5% was achieved on a medium containing of Murashige and Skoog (MS) inorganic salts and Linsmaier and Skoog (LS) vitamins, 2 mg/L of TDZ and 0.1 mg/L of IBA. Leaf tissue of 'Campbell Early', was co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, gus gene as reporter gene and resistance to kanamycin as selective agent, the other Agrobacterium strains, GV3101 containing the vector pB7 WG2D carrying with mPAP1-D gene. mPAP1-D is a regulatory genes of the anthocyanin biosynthetic pathway. 'Campbell Early' harboring mPAP1-D gene was readily able to be selected by red color due to anthocyanin accumulation in the transformed shoot. These results might be helpful for further studies to enhance the transformation efficiency in grape.
Isoflavones and biotransformed dihydrodaidzein production with in vitro cultured callus of Korean wild arrowroot Pueraria lobata
Lee, Eunji ; Kwon, Jung Eun ; Kim, Soojung ; Cha, Min-Seok ; Kim, Inhye ; Kang, Se Chan ; Park, Tae-Ho ;
Journal of Plant Biotechnology, volume 40, issue 4, 2013, Pages 217~223
DOI : 10.5010/JPB.2013.40.4.217
Pueraria lobata, a medicinally important leguminous plant produces various isoflavones including puerarin, daidzin and daidzein which are metabolized to equol via dihydrodaidzein and tetrahydrodaidzein by the bacterial fermentation of natural isoflavone sources in human intestines. In this study, we described callus proliferation and isoflavone production in callus of Korean wild arrowroot and dihydrodaidzein biosynthesis in callus extract fermented with Pediococcus pentosaceus. Proliferation was the best at callus cultured in the medium containing 1.0 mg/L TDZ and 1.0 mg/L NAA at light condition for 12 days. Puerarin was significantly more produced at callus cultured in the medium containing 2.0 mg/L kinetin and 1.0 mg/L NAA at dark condition for 16 days, but daidzin and daidzein were not significant. Callus extract was successfully fermented with P. pentosaceus and dihydrodaidzein, which is one of equol precursors formed by biotransformation, was confirmed to be produced. These results will facilitate mass production of callus and isoflavones as equol precursors from Korean wild arrowroot and can be applied for the production of equol by biotransformation in vitro.
Effects of ascorbic acid, citric acid and silver nitrate on the growth of in vitro lily plantlets and reduction of browning
Roh, Hee Sun ; Lee, Sang Il ; Kang, Yun Im ; Kim, Mi Seon ; Kim, Jong Bo ;
Journal of Plant Biotechnology, volume 40, issue 4, 2013, Pages 224~230
DOI : 10.5010/JPB.2013.40.4.224
Lily is an important cut-flower in Korea and world as well due to it's a variety of flower colors and various sizes of flowers. To develop elite lily cultivars, conventional breeding techniques have been used so far. However, an introduction of tissue culture system in mass propagation of bulbs and regeneration of shoots with a high efficiency is prerequisite at this moment. Especially, growth of bulbs and shoots as well as reduction of browning is critical factor to proliferate bulbs with shoots of superior lines or cultivar in lily. For this purpose, we tried to test whether ascorbic acid, citric acid and silver nitrate in medium to facilitate growth of bulbs and shoots as well as reduction of browning of bulb scales in lily. As a result, ascorbic acid and silver nitrate showed no significant differences compared to control plants in the growth of bulbs and shoots. When bulb scales were treated with
of citric acid, formations of shoots and bulbs showed best result. While, bulb scale treated with
of citric acid showed the growth of shoot and root as well as increasing of fresh weight compared to other treatments. Regarding the reduction of browning,
of ascorbic acid showed the best result with the less than 2%. Although more experiments with several commercial varieties are needed in the future to establish mass propagation of bulbs of lily, results obtained in this study are supposed to provide the basic knowledge and contribution in tissue culture system of lily.