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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
Editor in Chief :
Volume & Issues
Volume 41, Issue 4 - Dec 2014
Volume 41, Issue 3 - Sep 2014
Volume 41, Issue 2 - Jun 2014
Volume 41, Issue 1 - Mar 2014
Selecting the target year
Metabolic engineering for biofortification of lipophilic antioxidants in plants
Kim, Eun-Ha ; Lee, Kyeong-Ryeol ; Kim, Jong-Bum ; Roh, Kyung Hee ; Kang, Han Chul ; Kim, Hyun Uk ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 169~179
DOI : 10.5010/JPB.2014.41.4.169
Intracellular antioxidants include low molecular weight scavengers of oxidizing species, and enzymes which degrade superoxide and hydroperoxides. Such antioxidants systems prevent oxidative damage to cellular component by scavenging free radicals and activated oxygen species. Hydrophobic scavengers are found in cell membrane where they interrupt chain reactions of lipid peroxidation. The three major lipophilic antioxidant classes for human health are carotenoids, vitamin E and coenzyme Q10. The biofortification of staple crops with these lipid soluble antioxidants is an attractive strategy to increase the nutritional quality of human food. Here, we have summarized the biosynthetic pathways of three lipid soluble antioxidants in plants and current status of genetic engineered plants for elevated levels of each lipophilic antioxidant.
Organization and function of shoot apical meristem affecting growth and development in plants
Lee, Kyu Bae ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 180~193
DOI : 10.5010/JPB.2014.41.4.180
In plants, a shoot apex has a small region known as the shoot apical meristem (SAM) having a group of dividing (initiating) cells. The SAM gives rise to all the groundabove structures of plants throughout their lifetime, and thus it plays important role in growth and development of plants. This review describes theories to explain the SAM organization and function developed over the last 250 years. Since in 1759 German botanist C. F. Wolff has described firstly the SAM, in 1858 Swiss botanist C. N
geli proposed the apical cell theory from the observation of a large single apical cell in the SAM of seedless vascular plants: however, this view was recognized to be unsuitable to seed plants. In 1868, German botanist J. Hanstein suggested the histogen theory: this concept subdividing the SAM into dermatogen, periblem, and plerome was unable to generally apply to seed plants. In 1924, German botanist A. Schmidt proposed the tunica-corpus theory from the examination of angiosperm SAM in which two parts show different planes of cell division: this theory was proved to be not suitable to gymnosperm SAM, not have stable surface tunica layer. In 1938, American botanist A. Foster described zones in gymnosperm SAM based on the cytohistologic differentiation and thus called it a cytohistological zonation theory. With works by E. Gifford, in 1954, this zonation pattern was demonstrated to be also applicable to angiosperm SAM. As another theory, in 1952 French botanist R. Buvat proposed the m
me d`attente (waiting meristem) theory: however, this concept was confuted because of its negation of function during vegetative growth phase to central initial cells. Rescent studies with Arabidopsis thaliana have found that formation and maintenance of the SAM are under the control of selected genes: SHOOTMERISTEMLESS (STM) gene forms the SAM, and WUSCHEL (WUS) and CLAVATA (CLV) genes function in maintaining the SAM; signaling between WUS and CLV genes act through a negative feedback loop.
Isolation and characterization of a monodehydroascorbate reductase gene in poplar (Populus alba × P. glandulosa)
Yoon, Seo-Kyung ; Park, Eung-Jun ; Bae, Eun-Kyung ; Choi, Young-Im ; Kim, Joon-Hyeok ; Lee, Hyoshin ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 194~200
DOI : 10.5010/JPB.2014.41.4.194
Monodehydroascorbate reductase (MDHAR) is an important enzyme that plays a role in the detoxification of reactive oxygen species (ROS) by maintaining reduced pool of ascorbate through recycling the oxidized form of ascorbate. In this study, we isolated a PagMDHAR1 gene from Populus alba
P. glandulosa, and investigated its expression characteristics. The PagMDHAR1 cDNA encodes a putative 434 amino acids containing FAD- and NAD(P)H-binding domains. Southern blot analysis indicated that a single nuclear gene encodes this enzyme. Northern hybridization analysis revealed that PagMDHAR1 is highly expressed in both suspension cells and flower tissues, while its expression levels were enhanced by drought, salt, cold, wounding and ABA. Therefore, PagMDHAR1 might be expressed in response to abiotic stress through the ABA-mediated signaling pathway in this poplar species, suggesting that the PagMDHAR1 plays an important role in the defense mechanisms against oxidative stress.
Inhibitory effects of extracts from Smilacina japonica on lipopolysaccharide induced nitric oxide and prostaglandin E
production in RAW264.7 macrophages
Nam, Jung-Hwan ; Seo, Jong-Taek ; Kim, Yul-Ho ; Kim, Ki-Deog ; Yoo, Dong-Lim ; Lee, Jong-Nam ; Hong, Su-Young ; Kim, Su-Jeong ; Sohn, Hwang-Bae ; Kim, Hyun-Sam ; Kim, Bo-Sung ; Lee, Kyung-Tea ; Park, Hee-Jhun ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 201~205
DOI : 10.5010/JPB.2014.41.4.201
Smilacina japonica is a localized common rhizomatous flowering plant, This plant is often used in Korean traditional systems of medicine as a remedy for migrain, diplegia, physical impurity, blood circulation, abscess and contusion. Generally drugs that are used for arthritis have antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has not been scientifically investigated so far. Therefore, the aim of this study was to investigate the anti-inflammatory potential of S. japonica using the ethanolic extract and its subfractions. To evaluate the anti-inflammatory effects, we examined the inflammatory mediators such as nitric oxide (NO) and prostaglandin
) on RAW 264.7 macrophages. Our results indicated that hexane fraction significantly inhibited the LPS induced NO and
production in the cells. The hexane fractions inhibitory activity for NO tests with
values showed in
values showed at
. Theseis result suggest a potential role of hexane fraction from S. japonica as source of anti-inflammatory agent.
Comparison of the nutritional compositions of oxidative stress-tolerant transgenic rice and conventional rice
Woo, Hee-Jong ; Shin, Kong-Sik ; Lim, Myung-Ho ; Park, Soon Ki ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 206~211
DOI : 10.5010/JPB.2014.41.4.206
Nutritional assessment of transgenic crops to improve safety evaluations is important for food production. An oxidative stress-tolerant rice was generated by stable insertion of the TC gene-a tocopherol cyclase isolated from tobacco-into the genome of a common variety of japonica colored rice. The nutritional composition of the brown rice grains from the transgenic TC line was compared with that of the parental rice cultivar Heugnambyeo and two different varieties of non-transgenic rice. The results indicate that the analyzed nutritional compositions of the brown grains from the transgenic TC line were within the range of values reported for other commercial lines, and measurements of nutritional compositions were equivalent to those of the non-transgenic rice.
Effect of Kelpak
on the promotion of in vitro rooting in transgenic rose plantlets
Lee, Su Young ; Kwon, O Hyeon ; Lee, Hye Jin ; Kim, Won Hee ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 212~215
DOI : 10.5010/JPB.2014.41.4.212
In order to promote in vitro rooting in SOD2-transgenic rose plantlets, which were not well rooted in a rooting medium (MS medium with NAA
), we dipped the plantlets into liquid
before placing them in the rooting medium. After 4 weeks,
significantly promoted in vitro rooting in the plantlets. Therefore,
can be used successfully to aid in the in vitro rooting of rose plantlets with roots that are not well-generated.
Effects of osmoticum treatments and shooting chances on the improvement of particle gun-mediated transformation in Phalaenopsis
Roh, Hee Sun ; Kim, Jong Bo ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 216~222
DOI : 10.5010/JPB.2014.41.4.216
This study was carried out to develop an efficient transformation protocol via particle bombardment with PLBs (protocorm-like bodies) in Phalaenopsis. To achieve this aim, osmoticum treatment and an increasing shooting chances in particle bombardment process were applied for this study. In addition, pCAMBIA3301: ORE7 vector which contains a herbicide-resistance bar gene as a selectable marker and ORE7 gene as a gene of interests were employed. With regard to the increasing chances of shooting in particle bombardment, double shooting was the best results with 1.5 ~ 2.5 times higher than those of a single or triple shooting treatment in the productioon of PPT (D-L-phosphinothricin)-resistant PLBs. However, regeneration rate of shoots in double shooting was not high as a single shooting. Further, double shooting showed 35 ~ 40% higher than that of a single shooting in the frequency of browning. Regarding effects of different osmotic treatments, combination of 0.2 M sorbitol with 0.2 M mannitol showed the best results in transformation efficiency, regeneration of transformants and reduction of browning. Putative transgenic Phalaenopsis plants were analyzed by PCR analysis and confirmed the presence of bar and ORE 7 gene. Also, real-time PCR was conducted by using 21 transgenic plants and showed only 4 plants had one copy of transgene; whereas, the other 17 plants had more than 2 copies of transgene. Transgenic phalaenopsis plants produced in this study were transferred to pots and flowered normally without morphological variations in flower and leaf.
Establishment of proliferation and regeneration system of PLBs in Phalaenopsis by treatments of a variety of types of medium, sucrose concentrations and anti-browning agents
Roh, Hee Sun ; Kim, Jong Bo ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 223~228
DOI : 10.5010/JPB.2014.41.4.223
To establish an efficient proliferation and regeneration of PLBs (protocorm-like bodies) of Phalaenopsis plants, a variety of propagation medium types, various concentraions of sucrose as well as liquid and solid type were tested in this study. Further, activated charcoal, citric acid and ascorbic acid were compared whether these agents are suppose to reduce the browning in culture process using PLBs of Phalanopsis plants. With regard to the proper propagation medium, VW medium showed 1.3 ~ 2 times highr than those of other medium in an index of increasing for fresh weight and 50% higher than those of other medium in the frequency of shoot regeneration. However, regarding liquid and solid types of culture, there were no significant differences in the proliferation of PLBs and regeneration of shoots from PLBs. In the experiment for a variety of sucrose concentrations (0 ~ 50 g/l), 10 g of sucrose showed 30 ~ 50% higher than other concentrations in increasing index and 10 ~ 50% higher in the regeneration of shoots from PLBs. Regarding the reduction of browning in tissue culture via PLBs of Phalaenopsis plants, 1 g of activated charcoal showed only 1.5% browning of PLBs cultured. Whereas, other treatments including citric acid and ascorbic acid showed 6 ~ 16% of browning of PLBs. Therefore, activated charcoal was selected as an efficient anti-browning agents for the culture of PLBs in Phalaenopsis plants. Using above-described results can be contibuted to the establishment of mass propagation system using PLBs of Phalaenopsis plants in the future.
Change of amino acids contents of Gastrodia elata Blume with harvest times and seed tuber
Kim, Hyun-Tae ; Park, Eung-Jun ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 229~235
DOI : 10.5010/JPB.2014.41.4.229
Gastrodia elata Blume, an achlorophyllous orchid plant, has been used in traditional medicine and harvests in spring and fall. Here we investigated the quantitative changes of amino acids in G. elata by harvest times and seed tubers. In the results, we found that there was not big difference in contents of total amino acids but the distribution of amino acids differed depending on harvest times. 19% of total amino acids were asparagin and valine, an essential amino acid, occupied 10% within total amino acids in the tubers harvested in October. Among amino acids in the tubers harvested in April, serine occupied 23% and arginine, an essential amino acid, occupied 10% within total amino acids in tuber. Interestingly, the use of sexually propagated seed tubers produced high concentration of total amino acids compared to vegetatively propagated seed tubers. As for sexually propagated seed tubers, essential amino acids contents similar to the tubes harvested in October and nonessential amino acids contents similar to the tubers harvested in April. In this study, we found that amino acids contents in G. elata tubers altered depending on various cultivation practices. Therefore if these results can be applied to food industry, the value of G. elata as a natural food resource will be enhanced to a great extent.
High-frequency regeneration by stem disc culture in selected clones of Populus euramericana
Cui, Hae-Yeon ; Lee, Hyo-Shin ; Oh, Chang-Young ; Han, Shim-Hee ; Lee, Kyung-Ju ; Lee, Hyun-Jeong ; Kang, Kyu-Seok ; Park, So-Young ;
Journal of Plant Biotechnology, volume 41, issue 4, 2014, Pages 236~241
DOI : 10.5010/JPB.2014.41.4.236
An efficient regeneration protocol for stem disc culture of Populus euramericana, which is important species for bioenergy resource in agroforestry, was established. The number of explants that were obtained and the number of explants that regenerated varied with the genotypes. However, in all the genotypes, stem disc culture produced more regenerated shoots than did in axillary bud culture. A comparison of the effects of cytokinin type and concentration on shoot regeneration in different explants (i.e., petiole, leaf, and root segments of P. euramericana) revealed that a concentration of
thidiazuron (TDZ) used on petiole segments resulted in the greatest shoot regeneration (95.83%). The hormonal requirements for the greatest shoot regeneration in the three explant types varied. Different concentrations of
were added separately to the medium to stop the yellowing and subsequent necrosis of the regenerated shoots. Lower concentrations (3 and
) of these compounds improved shoot regeneration and elongation, compared with the control. The in vitro-regenerated shoots were transferred to rooting medium and subsequently acclimatized. The highly efficient regeneration system of P. euramericana reported here can be used for mass propagation of this recalcitrant for regeneration, economically important tree species.