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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
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Volume & Issues
Volume 42, Issue 4 - Dec 2015
Volume 42, Issue 3 - Sep 2015
Volume 42, Issue 2 - Jun 2015
Volume 42, Issue 1 - Mar 2015
Selecting the target year
Tyrosine phosphorylation as a signaling component for plant improvement
Park, Youn-Il ; Yang, Hyo-Sik ; Oh, Man-Ho ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 277~283
DOI : 10.5010/JPB.2015.42.4.277
Plant genome analyses, including Arabidopsis thaliana showed a large gene family of plant receptor kinases with various extracellular ligand-binding domain. Now intensively studies to understand physiological and cellular functions for higher plant receptor kinases in diverse and complex biological processes including plant growth, development, ligands perception including steroid hormone and plant-microbe interactions. Brassinosteroids (BRs) as a one of well know steroid hormone are plant growth hormones that control biomass accumulation and also tolerance to many biotic and abiotic stress conditions and hence are of relevance to agriculture. BRI1 receptor kinase, which is localized in plasma membrane in the cell sense BRs and it bind to a receptor protein known as BRASSINOSTEROID INSENSITIVE 1 (BRI1). Recently, we reported that BRI1 and its co-receptor, BRI1-ASSOCIATED KINASE (BAK1) autophosphorylated on tyrosine residue (s) in vitro and in vivo and thus are dual-specificity kinases. Other plant receptor kinases are also phosphorylated on tyrosine residue (s). Post-translational modifications (PTMs) can be studied by altering the residue modified by directed mutagenesis to mimic the modified state or to prevent the modification. These approaches are useful to not only characterize the regulatory role of a given modification, but may also provide opportunities for plant improvement.
Development of molecular marker to select resistant lines and to differentiate the races related to powdery mildew in melon (Cucumis melo L.)
Kim, Hoy-taek ; Park, Jong-in ; Ishikawa, Tomoko ; Kuzuya, Maki ; Horii, Manabu ; Yashiro, Katsutoshi ; Nou, Ill-sup ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 284~289
DOI : 10.5010/JPB.2015.42.4.284
Powdery mildew (Podosphaera xanthii) commonly occurs in cultivated fields of melon (Cucumis melo L.). It inflicts a lot of damages. Therefore, breeding resistant lines is essential. Development of a resistant line by integrating resistance gene takes a long time. In addition, break down of developed resistance by generating new virulent fungus strains increases disease susceptibility. This phenomenon was related to races of powdery mildew. Therefore, it is important to develop a DNA marker to genetically analyze race-specific resistance genes of melon powdery mildew to breed resistant lines. To date, a total of 28 races of Podosphaera xanthii have been reported in the literature. In Japan, 10 races have been reported in the Ibaraki region. We developed a system to characterize the races of Podosphaera xanthii and confirmed eight out of those 10 races in the Ibaraki region. In Korea, only one race has been characterized to date. However, some different races were detected. Through genetic analysis of resistant lines and susceptible lines of powdery mildew, resistance genes of race1 (Pm-X, PXB, and Pm-R 1), race N1 (PXA), race 2 (Pm-w and Pm-R 2), race 3 (Pm-X3), and race 5 (Pm-X5 and Pm-R5) were identified in melon. These related genes of race 1, 3, N1, 5, and race 1, 2, 5 were located at linkage group II and V, respectively. In race 1, resistance gene was located in the linkage group XII. In addition, each race-specific marker related to specific resistance gene was developed. Using race information and race selection system obtained in this study, resistant line can be bred to develop resistant cultivar for several areas. Furthermore, this will make it more easily and economically to breed resistant lines by using selected markers.
Researches of pear tree (Pyrus spp.) genomics
Oh, Youngjae ; Shin, Hyunsuk ; Kim, Keumsun ; Han, Hyeondae ; Kim, Yoon-Kyeong ; Kim, Daeil ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 290~297
DOI : 10.5010/JPB.2015.42.4.290
Based on the place of its origin, pear tree (Pyrus spp.) is largely divided into European pears (P. communis, cultivated mainly in Europe and the U.S.) and Asian pears (P. pyrifolia, P. bretschneideri, and P. ussuriensis, distributed and grown in East Asian countries including China, Japan, and Korea). Most pear trees have 17 chromosomes (diploidy, 2n=2x=34). Their genetic studies and precise cultivar breeding are highly restricted by conditions such as self-incompatibility controlled by S-locus and juvenility as one major character of fruit crops. Genetic studies on Pyrus have been promoted by the development of various molecular markers. These markers are being utilized actively in various genetic studies, including genetic relationship analysis, genetic mapping, and QTL analysis. In addition, research on pear genetic linkage maps has been extended to studies for the identification of QTL for target traits such as disease resistance and genetic loci of useful traits. NGS technology has radically reduced sequencing expenses based on massive parallel reactions to enable high-capacity and high-efficiency. NGS based genome analyses have been completed for Chinese pear 'Danshansuli' and European pear 'Bartlett'. In Korea, GWAS for agricultural valuable traits such as floral structure, ripening, and total soluble contents have been conducted through resequencing. GBS has been performed for 'Whangkeumbae', 'Cheongsilri', and 'Minibae'.
Current status and prospects of genomics and bioinformatics in grapes
Hur, Youn Young ; Jung, Sung Min ; Yun, Hae Keun ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 298~311
DOI : 10.5010/JPB.2015.42.4.298
Grape is one of the important fruit crops around the world, and exposed to disease and pests, and internal or environmental stresses in the vineyards. Breeding and cultivation of new varieties of high quality-grapes resistant to diseases and pests and tolerant to stresses are the most important steps in the grape production. However, conventional breeding has laborious and time-consuming procedures in maintaining and selecting seedlings in the fields. Development of molecular breeding technology through understanding of molecular mechanism of useful traits can be used as an alternative strategy to improve the efficiency of grape breeding program by cross hybridization in grape development programs. The completion of the grape genome sequencing project provided the way to discover the novel genes and to analyze their functions. Comparative genomics, transcriptomic analysis, and the genome-wide identification and analysis of useful genes as well as development of molecular marker for valuable traits could provide novel insights into fruit quality and the responses to diseases and stresses, and can be used as important information in molecular breeding programs for grape development.
Current status of peach genomics and transcriptomics research
Cho, Kang Hee ; Kwon, Jung Hyun ; Kim, Se Hee ; Jun, Ji Hae ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 312~325
DOI : 10.5010/JPB.2015.42.4.312
In this review, we summarized the trends of genomics and transcriptomics research on peach, a model species of Rosaceae. Peach genome maps have been developed from various progeny groups with many next-generation sequencing (NGS) based single nucleotide polymorphism markers. Molecular markers of qualitative traits and quantitative trait loci (QTL) such as fruit characteristics, blooming date, and disease resistance have been analyzed. Among many characteristics, markers related to flesh softening and flesh adhesion are useful for marker assisted selection. Through comparative genomics, peach genome has been compared to the genome of Arabidopsis, Populus, Malus, and Fragaria species. Through transcriptomics and proteomics, fruit growth and development, and flavonoid synthesis, postharvest related transcriptomes and disease resistance related proteins have been reported. Recently, development of NGS based markers, construction of core collection of germplasm, and genotyping of various progenies have been preceded. In the near future, accurate QTL analysis and identification of useful genes are expected to establish a foundation for effective molecular breeding.
Current status and prospects of citrus genomics
Kim, Ho Bang ; Lim, Sanghyun ; Kim, Jae Joon ; Park, Young Cheol ; Yun, Su-Hyun ; Song, Kwan Jeong ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 326~335
DOI : 10.5010/JPB.2015.42.4.326
Citrus is an economically important fruit tree with the largest amount of fruit production in the world. It provides important nutrition such as vitamin C and other health-promoting compounds including its unique flavonoids for human health. However, it is classified into the most difficult crops to develop new cultivars through conventional breeding approaches due to its long juvenility and some unique reproductive biological features such as gamete sterility, nucellar embryony, and high level of heterozygosity. Due to global warming and changes in consumer trends, establishing a systematic and efficient breeding programs is highly required for sustainable production of high quality fruits and diversification of cultivars. Recently, reference genome sequences of sweet orange and clementine mandarin have been released. Based on the reference whole-genome sequences, comparative genomics, reference-guided resequencing, and genotyping-by-sequencing for various citrus cultivars and crosses could be performed for the advance of functional genomics and development of traits-related molecular markers. In addition, a full understanding of gene function and gene co-expression networks can be provided through combined analysis of various transcriptome data. Analytic information on whole-genome and transcriptome will provide massive data on polymorphic molecular markers such as SNP, INDEL, and SSR, suggesting that it is possible to construct integrated maps and high-density genetic maps as well as physical maps. In the near future, integrated maps will be useful for map-based precise cloning of genes that are specific to citrus with major agronomic traits to facilitate rapid and efficient marker-assisted selection.
Current status and prospects of blueberry genomics research
Kim, Jin Gook ; Yun, Hae Keun ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 336~341
DOI : 10.5010/JPB.2015.42.4.336
Blueberry (Vaccinium spp.) is a bush that grows well at special cultural environments such as acid soil, high organic matter content, and a good drainage and aeration compared to other general crops. Blueberries are well known to contain high amounts of anthocyanins and phenolic compounds, resulting in high antioxidant activity that provides health benefits, and expanding the cultivation areas and consumer's demand in the worldwide. However, the full genome of blueberry has not been announced until now. Furthermore, the genomic analysis and transcriptome approaches are not so popular compare to major crops such as orange, apple, and grape. The aim of the review about blueberry genomic research is to establish the platform for setting blueberry breeding target, increasing proficiency of blueberry research, and making the practical cultivation techniques in Korea. The main topics in the blueberry genomic research including transcriptome, genetic mapping, and various markers are related with cold hardiness, chilling requirement, hot tolerance, anthocyanin content, and flavonoid synthesis pathway on various tissues like flower bud, leaf bud, shoot, root, and berry fruit. The review of the current status of blueberry genomic research will provide basic information to the breeders and researchers and will contribute to development of blueberry industry with sustainable productions and increase of blueberry consumption as new profitable crops in Korea.
Current status and prospects of kiwifruit (Actinidia chinensis) genomics
Kim, Seong-Cheol ; Kim, Ho Bang ; Joa, Jae-Ho ; Song, Kwan Jeong ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 342~349
DOI : 10.5010/JPB.2015.42.4.342
Kiwifruit is a new fruit crop that was commercialized in the late 1970s. Recently, its cultivation and consumption have increased rapidly worldwide. Kiwifruit is a dioecious, deciduous, and climbing plant having fruit with hairs and various flesh colors and a variation in ploidy level; however, the industry consists of very simple cultivars or genotypes. The need for efficient cultivar improvement together with the evolutional and biological perspectives based on unique plant characteristics, have recently encouraged genome analysis and bioinformatics application. The draft genome sequence and chloroplast genome sequence of kiwifruit were released in 2013 and 2015, respectively; and gene annotation has been in progress. Recently, transcriptome analysis has shifted from previous ESTs analysis to the RNA-seq platform for intensive exploration of controlled genetic expression and gene discovery involved in fruit ascorbic acid biosynthesis, flesh coloration, maturation, and vine bacterial canker tolerance. For improving conventional breeding efficiency, molecular marker development and genetic linkage map construction have advanced from basic approaches using RFLP, RAPD, and AFLP to the development of NGS-based SSR and SNP markers linked to agronomically important traits and the construction of highly saturated linkage maps. However, genome and transcriptome studies have been limited in Korea. In the near future, kiwifruit genome and transcriptome studies are expected to translate to the practical application of molecular breeding.
Root proteome analysis of Chinese cabbage in response to Plasmodipohora brassicae Woron
Jeung, Jae Yun ; Lim, Yong Pyo ; Hwang, Cheol Ho ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 350~355
DOI : 10.5010/JPB.2015.42.4.350
Clubroot disease is one of the most wide-spread and devastating diseases in the cultivation of Chinese cabbage. To develop a protein marker for resistance to clubroot disease in Chinese cabbage, a comparative proteome analysis was performed between a sensitive line, 94SK, and a resistant line, CR Shinki DH. Three proteins of two fold or higher accumulation that are specific to each line were found 3 days after innoculation of the Plasmodiphora brassicae. They are glutamine synthetase, malate dehydrogenase/oxidoreductase and fructose-bisphosphate aldolase in the 94SK and actin, phosphoglycerate kinase, and Cu/Zn superoxide dismutase in the CR Shinki line. From the comparison of the synthesized proteins in the 94SK and the CR Shinki, CR Shinki was found to produce more ATP-binding protein for the ABC transporter while 94SK showed a higher level of pathogenesis-related protein 1 production. All of these proteomic variations may lead to the development of molecular markers to accelerate the breeding process.
Characterization of a non-specific Lipid Transfer Protein (ns-LTP) promoter from poplar (Populus alba × P. glandulosa)
Cho, Jin-Seong ; Noh, Seol Ah ; Choi, Young-Im ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 356~363
DOI : 10.5010/JPB.2015.42.4.356
In order to study genetic engineering in trees, the characterization of genes and promoters from trees is necessary. We isolated the promoter region (867 bp) of Pagns-LTP from poplar (P. alba
P. glandulosa) and characterized its activity in transgenic poplar plants using a
-glucuronidase (GUS) reporter gene. High-level expression of the Pagns-LTP transcript was found in poplar roots, while comparatively low-level expression was found in the young leaves. Pagns-LTP mRNA was not detected in other poplar tissues. Additionally, transgenic poplar plants that contained a Pagns-LTP promoter fused to a GUS reporter gene, displayed tissue-specific GUS enzyme activity localized in root tissue. In silico analysis of the Pagns-LTP promoter sequence reveals the presence of several cis-regulatory elements responsive to phytohormones, biotic and abiotic stresses, as well as those regulating tissue-specific expression. These results demonstrate that the Pagns-LTP promoter has tissue-specific expression activity in poplar roots and leaves that may be involved in organ development and plant resistance to various stresses. Therefore, we anticipate that the Pagns-LTP promoter would be a useful tool to genetically optimize woody plants for functional genomics.
Serotonins of safflower seeds play a key role in anti-inflammatory effect in lipopolysaccharide-stimulated RAW 264.7 macrophages
Kim, Dong-Hee ; Moon, Yong-Sun ; Park, Tae-Soon ; Son, Jun-Ho ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 364~369
DOI : 10.5010/JPB.2015.42.4.364
Safflower (Carthamus tinctorius) seeds are wellknown traditional oriental medicines that have long been used for the remedies of blood stasis and bone formation in east Asia. In this study, ethyl acetate (EtOAc) was used for extraction of the main chemical compounds from C. tinctorius seeds. Four major compounds were identified, acacetin, cosmosiin, N-feruloyl serotonin and N-(p-coumaroyl) serotonin. Each compound was evaluated for its inhibitory activity against the inflammatory process of macrophages. All compounds significantly inhibited production of lipopolysaccharide (LPS)-stimulated nitric oxide (NO) and pro-inflammatory cytokines. The protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were dramatically decreased by serotonins in a dose-dependent manner in LPS-stimulated RAW 264.7 macrophages. These results suggest that serotonin derivatives from safflower seeds may reduce inflammation-related diseases.
Factors influencing shoot regeneration from petal explant in spray mum 'Purple ND'
Lee, Hyun Suk ; Park, Hyun Rho ; Kim, Hyun seak ; Kim, Chang Kil ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 370~375
DOI : 10.5010/JPB.2015.42.4.370
This experiment compared the regeneration conditions of the radiation mutant spray chrysanthemum 'purple ND'. The four different flower blooming stages (S1: 10% opened flower, S2: 30% opened flower, S3: 50% opened flower, and S4: 70% opened flower) and different petal parts (TBOP: the basal of petal and TEOP: the end of petal) were used to compare regeneration conditions between plants grown in MS medium supplemented with IAA and BAP. The highest adventitious shooting rate was identified in plants grown on the IAA
when using the end of petal at the S2 stage. It displayed 79.2% regeneration and produced 33.4 shoots. Rooted plantlets were successfully established in the greenhouse, showing the same morphological characteristics of vegetative and reproductive organs with those of the mother plant. Flow cytometry analysis revealed no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions.
Effect of tissue proliferation and somatic embryo induction in Larix kaempferi following treatment with organic nitrogen sources and plant growth regulators
Kim, Yong Wook ; Kim, Ji Ah ; Moon, Heung Kyu ; Jeong, Su Jin ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 376~379
DOI : 10.5010/JPB.2015.42.4.376
This study was conducted to evaluate the effects of different types and concentrations of organic nitrogen sources (
-Glutamine and casein hydrolysate, CH) and plant growth regulators (auxins and cytokinins) on embryogenic tissue proliferation and somatic embryo production in L. kaempferi. Overall, the highest tissue fresh weight was obtained at either 2 or 4 weeks in culture when 1,000 mg/L
-Glutamine was added to the culture medium, which showed similar results with other treatments. In experiments with different types and concentrations of plant growth regulators on somatic embryo production, the highest production (426.3/90 mg tissue) was found when 0.2 mg/L IBA was added; however, no somatic embryos were induced following treatment with 0.2 mg/L BA or Kinetin. The effect of various concentrations of IBA on somatic embryo production was also tested. The best result (303/90 mg tissue) was obtained when plants were treated with 0.2 mg/L IBA; 1.0 mg/L IBA was also effective (281/90 mg tissue). The lowest result (109.3/90 mg tissue) was obtained with 5.0 mg/L IBA.
Micropropagation of Aronia (Aronia melaocarpa Elliot, black chokeberry) and its 5 varieties
Kwak, Myoung-Chul ; Choi, Chung-Ho ; Choi, Yong-Eui ; Moon, Heung-Kyu ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 380~387
DOI : 10.5010/JPB.2015.42.4.380
Aronia (Aronia melanocarpa, Black chokeberry) is an important cash crop in domestic agriculture. We investigated the effects of plant growth regulators on shoot proliferation and rooting using in vitro tissue culture. The most effective shoot multiplication was observed on WPM (woody plant medium) supplemented with 1.0 mg/L zeatin (
shoots/explant), while the highest rooting rate was obtained from half-strength WPM with 3.0 mg/L IBA (8.8 roots/explant). The rooted plantlets all survived in the artificial soil mixture (with a mixture of peat moss : perlite : vermiculite, 1:1:1, v/v/v) and grew up relatively uniform, ranging from 14 to 16 leaves, 8 to 10 cm in stem height, and 2.3 to 2.8 mm in stem diameter. While experimenting with 5 different varieties of Aronia, we found out that each variety had different characteristics of shoot proliferation and rooting. The total numbers of proliferated shoots per variety is as follows:
for Nero, 14 to 15 for Purple and Mackenzie, and 10 for both Viking and Odamamachiko. Rooting rates were also various depending on the variety: 88% of Odamamachiko, 80% of Viking and Purple, and 76% of Nero and 60% of Mackenzie shoots rooted. The survival rate of the rooted plantlets was from 92% to 100%, varying by type. Further growth appeared to be better in auxin-treated plantlets, compared to untreated ones. Our results showed the possibility of establishing an effective in vitro micropropagation system for Aronia melanocarpa.
Somatic embryo induction and plant regeneration from cold-stored embryogenic callus of K. septemlobus
Lee, Na Nyum ; Choi, Yong Eui ; Moon, Heung Kyu ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 388~395
DOI : 10.5010/JPB.2015.42.4.388
Somatic embryogenesis is as an excellent technology for potential use in plant mass production, germplasm conservation, or genetic engineering. We examined the effect of cold storage using 3 embryogenic callus lines with different levels of embryogenesis competence derived from immature zygotic embryo cultures of Kalopanax setemlobus. Somatic embryo induction, germination and plant conversion were evaluated after 1, 3 and 6 months storage at
in the dark. Most cold-stored embryogenic calli formed somatic embryos normally even after 6 months; however, the induction rate was gradually decreased by increasing the storage period. The most competent line tended to show a slight decline in somatic embryo induction rate, as compared with other lines after cold storage. In general, cold storage resulted in reduced somatic embryo germination and plant regeneration, although 93% somatic embryo germination and 91% plant conversion were achieved regardless of the storage period. Cold storage led to cell browning and degradation. Additionally, the cell structures were confirmed by the aceto-carmine and evans blue dye evaluation. Collectively, our results showed that embryogenic callus of K. septemlobus could be preserved at
without subculture for 6 months, and suggested the need for storage of relatively more competent embryogenic calli lines to support somatic embryo induction.
Plant regeneration from hypocotyls explants of Astragalus sinicus L.
Park, Min Sun ; Choi, Pil Son ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 396~400
DOI : 10.5010/JPB.2015.42.4.396
To investigate the optimal conditions for shoot organogenesis in Astragalus sinicus L., hypocotyl explants were cultured in Murashige & Skoog's (MS) medium supplemented with 0.1, 1.0, 2.0, or 4.0 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D) for 6 weeks. 2,4-D concentration significantly effected morphogenesis: some produced calli with adventitious shoots and roots, some produced calli with adventitious roots, some produced only calli, and some produced deep-brownish calli with roots. The formation of calli with shoots and/or roots was observed at lower levels of 2,4-D, whereas calli without shoots or with deep-brownish roots were formed after treatment with higher levels of 2,4-D. Also, a shoot organogenesis ability of callus clones was observed after treatment with medium with 0.1 or 1.0 mg/L 2,4-D grown in MS medium with combinations of benzyl adenine (BA) and 2,4-D for 4 weeks. Medium with a combination of BA and 2,4-D was effective for shoot formation, whereas root organogenesis from calli decreased. The greatest amount of shoot formation was obtained when calli were cultured in MS medium containing 1.0 mg/L 2,4-D and 0.5 mg/L BA. Upon shoot transfer into 1/2 MS basal medium, plantlets developed, and the plantlets grew well in soil in a greenhouse.
Evaluation of horticultural traits and genetic relationship in melon germplasm
Jung, Jaemin ; Choi, Sunghwan ; Oh, Juyeol ; Kim, Nahui ; Kim, Daeun ; Son, Beunggu ; Park, Younghoon ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 401~408
DOI : 10.5010/JPB.2015.42.4.401
Horticultural traits and genetic relationship were evaluated for 83 melon (Cucumis melo L.) cultivars. Survey of a total of 36 characteristics for seedling, leaf, stem, flower, fruit, and seed and subsequent multiple analysis of variance (MANOVA) were conducted. Principal component analysis (PCA) showed that 8 principle components including fruit weight, fruit length, fruit diameter, cotyledon length, seed diameter, and seed length accounted for 76.3% of the total variance. Cluster analysis of the 83 melon cultivars using average linkage method resulted in 5 clusters at coefficient of 0.7. Cluster I consisted of cultivars with high values for fruit-related traits, Cluster II for soluble solid content, and Cluster V for high ripening rate. Genotyping of the 83 cultivars was conducted using 15 expressed-sequence tagged-simple sequence repeat (EST-SSR) from the Cucurbit Genomics Initiative (ICuGI) database. Analysis of genetic relatedness by UPGMA resulted in 6 clusters. Mantel test indicated that correlation between morphological and genetic distance was very low (r = -0.11).
Studies on nickel uptake in transgenic Arabidopsis thaliana introduced with TgMTP1 gene encoding metal tolerance protein
Kim, Donggiun ;
Journal of Plant Biotechnology, volume 42, issue 4, 2015, Pages 409~413
DOI : 10.5010/JPB.2015.42.4.409
To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both
(T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that
-overexpressing plants may be useful for phytoremediation of soil.