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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of Plant Biotechnology
Journal Basic Information
Journal DOI :
The Korean Society of Plant Biotechnology
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Volume & Issues
Volume 43, Issue 2 - Jun 2016
Volume 43, Issue 1 - Mar 2016
Selecting the target year
Global status of GM crop development and commercialization
Cho, Jung-Il ; Lee, Gang-Seob ; Park, Soo-Chul ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 147~150
DOI : 10.5010/JPB.2016.43.2.147
Global GM crops continue to grow. They have reached 181 million hectares. A total of 28 countries have approved biotech crops for planting. More than 60 countries have approved biotech crops to be imported as food and feed since 1996, meaning that biotech crops are now commonly accepted in those countries. Although biotech crops provide key solutions for the challenge of global food security in the future due to population growth and climate change, there are still some debates on whether biotech crops should be accepted in many countries including Korea. Therefore, it is very important to make people understand that GM crops will provide benefits to both farmers and consumers. In this review, current global status of GM crop development and commercialization are summarized.
Current status and prospects of the authentication of Angelica species
Gil, Jinsu ; Park, Sang ik ; Lee, Yi ; Kim, Ho Bang ; Kim, Seong-Cheol ; Kim, Ok-Tae ; Cha, Seon-Woo ; Jung, Chan Sik ; Um, Yurry ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 151~156
DOI : 10.5010/JPB.2016.43.2.151
Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, natural cosmetics, and pharmaceutical industries. However, names are different from each country and their phylogenetic origins are not clear. These lead consumers to be confused. In particular, when they are morphologically similar and distributed as dried roots, it is extremely difficult to differentiate their origins even by specialists. Recently, molecular markers have been extensively applied to identify the origin of many crops. In this review, we tried to overview the current research achievements for the development of suitable `origin identification` regarding to the differentiation of Angelica species. Furthermore, more advanced techniques including amplification genome based marker analyses are also discussed for their practical applications in the authentication of particular medicinal plant in Angelica species.
Genetic variation of halophyte New Zealand spinach (Tetragonia tetragonioides) accessions collected in Korea using an AFLP marker
Jeon, Yongsam ; Jin, Yong-Tae ; Choi, Seo-Hee ; Park, Nuri ; Kim, In-Kyung ; Lee, Ka Youn ; Choi, Jong-Jin ; Lee, Geung-Joo ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 157~163
DOI : 10.5010/JPB.2016.43.2.157
This study was conducted to investigate the potential use of New Zealand spinach (Tetragonia tetragonioides) as a new vegetable crop which will be cultivated in salt-affected soils such as reclaimed areas. New Zealand spinach ecotypes native to Korea were collected across the Southern, Western and Eastern seashore regions of the Korean peninsula, among which fifty-five accessions were later further propagated and evaluated genetically by using an AFLP (amplified fragment length polymorphism) marker. Based on the AFLP analysis performed to uncover the genetic diversity of the collected ecotypes, enzymatic cleavage of the extracted DNA was implemented based on 12 EcoRI and MseI combinations. A total of 1,279 alleles (107 alleles per EcoRI and MseI enzyme combination) were successfully amplified, among which 62 alleles per enzyme combination were polymorphic (58%). The AFLP analysis indicated that the rate of genetic dissimilarity was 29% among the New Zealand spinach collections, which were clustered into the 7 genetic diversity group. This is the first report on the genetic variation in the genus Tetragonia, and the basic information can be applied to select parental lines for enhancing the segregation spectrum of the new halophytic vegetable plant grown in salt-affected areas.
Genome-wide analysis of sequence variations in eight inbred watermelon lines
Kim, Youn-Sung ; Ko, Chan-Sup ; Yang, Hee-Beom ; Kang, Sun-Chul ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 164~173
DOI : 10.5010/JPB.2016.43.2.164
To investigate the genetic basis of phenotypic differences, sequence variations were analyzed in 8 inbred watermelon lines by re-sequencing. The number of sequence variations differed depending on the chromosome. Only 12.9% of SNPs were found within genes, whereas the rest were detected in promoter or intergenic regions. SNP density analysis showed that there was a highly variable region at the end of chromosome 6, which is similar to previously published findings. However, this region with high SNP density did not show much variation between the lines. In contrast, highly conserved regions with a size of 6.5-10 Mb were found in chromosomes 10 and 11. Pathway analysis suggested that the DIMBOA (a natural antibiotic)-glucoside degradation pathway was significantly different between the lines, indicating that the eight lines may have different levels of pathogen resistance. Among the carbohydrate-related genes, the alpha-galactosidase gene was the most variable among the lines. Information from this study will be helpful in understanding the watermelon breeding process at the molecular level.
Genetic diversity analysis of Glycyrrhiza uralensis using 8 novel polymorphic microsatellite markers
Um, Yurry ; Jin, Mei-Lan ; Lee, Yi ; Hur, Mok ; Cha, Seon Woo ; Jung, Chan Sik ; Kim, Seong Min ; Lee, Jeong-Hoon ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 174~180
DOI : 10.5010/JPB.2016.43.2.174
Licorice plant (Glycyrrhiza spp.) is an important herb, but the major portion of the national demand is imported to Korea because the domestic production base is vulnerable. We performed basic molecular breeding research for domestic cultivation and production. All publicly available G. uralensis EST sequences, which totaled 56,089, were assembled into 4,821 unigenes and examined for microsatellites. Eight polymorphic microsatellite loci were identified and 16 G. uralensis and 6 G. glabra accessions, which were collected from different locations, were genotyped using the microsatellites. Genetic diversity within the accessions was estimated by construction of a dendrogram. The dendrogram was clustered into two groups. The results showed that there is a correlative genetic relationship between species. The microsatellite markers were found to be useful for diversity analysis as they are able to successfully distinguish the Glycyrrhiza accessions.
Simple sequence repeat marker development from Codonopsis lanceolata and genetic relation analysis
Kim, Serim ; Jeong, Ji Hee ; Chung, Hee ; Kim, Ji Hyeon ; Gil, Jinsu ; Yoo, Jemin ; Um, Yurry ; Kim, Ok Tae ; Kim, Tae Dong ; Kim, Yong-Yul ; Lee, Dong Hoon ; Kim, Ho Bang ; Lee, Yi ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 181~188
DOI : 10.5010/JPB.2016.43.2.181
In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.
In silico analysis of MeJA-induced comparative transcriptomes in Brassica oleraceae L. var. capitata
Lee, Ok Ran ; Kim, Dae-Soo ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 189~203
DOI : 10.5010/JPB.2016.43.2.189
Brassica oleraceae var capitata is a member of the Brassicaceae family and is widely used as an horticultural crop. In the present study, transcriptome analysis of B. oleraceae L. var capitata was done for the first time using eight-week old seedlings treated with
MeJA, versus mock-treated samples. The complete transcripts for both samples were obtained using the GS-FLX sequencer. Overall, we obtained 275,570 and 266,457 reads from seedlings treated with or without
MeJA, respectively. All the obtained reads were annotated using biological databases and functionally classified using gene ontology (GO), the Kyoto Encyclopedia of Genes and Genomics (KEGG). By using GO analyses, putative transcripts were examined in terms of biotic and abiotic stresses, cellular component organization, biogenesis, and secondary metabolic processes. The KEGG pathways for most of the transcripts were involved in carbohydrate metabolism, energy metabolism, and secondary metabolite synthesis. In order to double the sequenced data, we randomly chose two putative genes involved in terpene biosynthetic pathways and studied their transcript patterns under MeJA treatment. This study will provide us a platform to further characterize the genes in B. oleracea var capitata.
Transcriptomic analysis of `Campbell Early` and `Muscat Bailey A` grapevine shoots exposed to freezing cold stress
Kim, Seon Ae ; Yun, Hae Keun ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 204~212
DOI : 10.5010/JPB.2016.43.2.204
To understand the responses of grapevines in response to cold stress causing the limited growth and development, differentially expressed genes (DEGs) were screened through transcriptome analysis of shoots from 2 grapevine cultivars (`Campbell Early` and `Muscat Baily A`) kept at -
for 4 days. In gene ontology analysis of DEGs from `Campbell Early`, there were 17,424 clones related with biological process, 28,954 with cellular component, and 6,972 with molecular function genes in response to freezing temperature. The major induced genes included dehydrin xero 1, K-box region and MADS-box transcription factor family protein, and MYB domain protein 36, and inhibited genes included light-harvesting chlorophyll B-binding protein 3, FASCICLIN-like arabinoogalactan 9, and pectin methylesterase 61 in `Campbell Early` grapevines. In gene ontology analysis of DEGs from `Muscat Baily A`, there were 1,157 clones related with biological process, 1,350 with cellular component, and 431 with molecular function gene. The major induced genes of `Muscat Baily A` included NB-ARC domain-containing disease resistance protein, fatty acid hydrozylase superfamily, and isopentenyltransferase 3, and inhibited genes included binding, IAP-like protein 1, and pentatricopeptide repeat superfamily protein. All major DEGs were shown to be expressed differentially by freezing temperature in real time-PCR analysis. Protein domain analysis using InterPro Scan revealed that ubiquitin-protein ligase was redundant in both tested grapevines. Transcriptome profile of shoots exposed to cold can provide new insights into the molecular basis of tolerance to low-temperature in grapevines, and can be used as resources for development new grapevines tolerant to coldness.
Rapid metabolic discrimination between Zoysia japonica and Zoysia sinica based on multivariate analysis of FT-IR spectroscopy
Yang, Dae-Hwa ; Ahn, Myung Suk ; Jeong, Ok-Cheol ; Song, In-Ja ; Ko, Suk-Min ; Jeon, Ye-In ; Kang, Hong-Gyu ; Sun, Hyeon-Jin ; Kwon, Yong-Ik ; Kim, Suk Weon ; Lee, Hyo-Yeon ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 213~222
DOI : 10.5010/JPB.2016.43.2.213
This study aims to establish a system for the rapid discrimination of Zoysia species using metabolite fingerprinting of FT-IR spectroscopy combined with multivariate analysis. Whole cell extracts from leaves of 19 identified Zoysia japonica, 6 identified Zoysia sinica, and 38 different unidentified Zoysia species were subjected to Fourier transform infrared spectroscopy (FT-IR). PCA (principle component analysis) and PLS-DA (partial least square discriminant analysis) from FT-IR spectral data successfully divided the 25 identified turf grasses into two groups, representing good agreement with species identification using molecular markers. PC (principal component) loading values show that the
region of the FT-IR spectra are important for the discrimination of Zoysia species. A dendrogram based on hierarchical clustering analysis (HCA) from the PCA and PLS-DA data of turf grasses showed that turf grass samples were divided into Zoysia japonica and Zoysia sinica in a species-dependent manner. PCA and PLS-DA from FT-IR spectral data of Zoysia species identified and unidentified by molecular markers successfully divided the 49 turf grasses into Z. japonica and Z. sinica. In particular, PLS-DA and the HCA dendrogram could mostly discriminate the 47 Z. japonica grasses into two groups depending on their origins (mountainous areas and island area). Considering these results, we suggest that FT-IR fingerprinting combined with multivariate analysis could be applied to discriminate between Zoysia species as well as their geographical origins of various Zoysia species.
Proteomic analysis of dehydroascorbate reductase transgenic potato plants
Han, Eun-Heui ; Goo, Young-Min ; Kim, Yun-Hee ; Lee, Shin-Woo ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 223~230
DOI : 10.5010/JPB.2016.43.2.223
Ascorbic acid (AsA) is a strong antioxidant/reducing agent that can be converted to dehydroascorbate (DHA) by oxidation in plants. DHA, a very short-lived chemical, is recycled to AsA by dehydroascorbate reductase (DHAR). Previously, DHAR cDNA was isolated from the hairy roots of the sesame plant, and DHAR-overexpressing transgenic potato plants were generated under the control of the CaMV35S promoter (CaMV35S::DHAR). An increase in transgene expression and ascorbate levels were observed in the transgenic plants. In the present study, proteomic analysis revealed that transgenic plants not only accumulated DHAR in their cells, but also induced several other antioxidant enzyme-related proteins during plant growth. These results suggest that DHAR is important for stress tolerance via induction of antioxidant proteins, and could improve stress tolerance in transgenic potato plants.
DNA microarray analysis of RNAi plant regulated expression of NtROS2a gene encoding cytosine DNA demethylation
Choi, Jang Sun ; Lee, In Hye ; Jung, Yu Jin ; Kang, Kwon Kyoo ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 231~239
DOI : 10.5010/JPB.2016.43.2.231
To study the transcript levels of epigenetically regulated genes in tobacco, we have developed a transgenic line OX1 overexpressing NtROS2a gene encoding cytosine DNA demethylation and a RNAi plant line RNAi13. It has been reported that salt- and
-stress tolerance of these transgenic lines are enhanced with various phenotypic characters (Lee et al. 2015). In this paper, we conducted microarray analysis with Agilent Tobacco 4 x 44K oligo chip by using overexpression line OX1, RNAi plant line RNAi 13, and wild type plant WT. Differentially expressed genes (DEGs) related to metabolism, nutrient supply, and various stressed were up-regulated by approximately 1.5- to 80- fold. DEGs related to co-enzymes, metabolism, and methylation functional genes were down-regulated by approximately 0.03- to 0.7- fold. qRT-PCR analysis showed that the transcript levels of several candidate genes in OX1 and RNAi lines were significantly (p < 0.05) higher than those in WT, such as genes encoding KH domain-containing protein, MADS-box protein, and Zinc phosphodiesterase ELAC protein. On the other hand, several genes such as those encoding pentatricopeptide (PPR) repeat-containing protein, histone deacetylase HDAC3 protein, and protein kinase were decreased by approximately 0.4- to 1.0- fold. This study showed that NtROS2a gene encoding DNA glycosylase related to demethylation could regulate adaptive response of tobacco at transcriptional level.
A novel method for high-frequency transgenic shoot regeneration via Agrobacterium tumefaciens in flax (Linum usitatissimum L.)
Beyaz, Ramazan ; Darcin, E. Selcen ; Aycan, Murat ; Kayan, Mustafa ; Yildiz, Mustafa ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 240~247
DOI : 10.5010/JPB.2016.43.2.240
In this study, routinely used transformation method, which includes transferring explants onto co-cultivation medium after inoculating them with bacterial solution for a while, was compared with 3 different inoculation methods. In every 3 methods, hypocotyl explants excised from 7-day-old sterile flax seedlings having cotyledon leaves and no root system dried under air flow in sterile cabin for 35 min were inoculated with different volumes of bacterial solution at different inoculation periods. GV2260 line of Agrobacterium tumefaciens having `pBIN 19` plasmid containing npt II (neomycin phosphotransferase II) gene and GUS reporter gene was used in transformation studies. After inoculation, hypocotyl segments of seedlings (0.5 cm in length) - were excised and left to co-cultivation for 2 days. Then, explants were transferred to regeneration medium supplemented with different antibiotics. The presence of npt-II and GUS genes in transformants was confirmed by PCR and GUS analysis. The highest results in all characters examined in all cultivars were obtained from the 2 inoculation method in which hypocotyls excised from seedlings inoculated with
of bacterial solution after drying in sterile cabin for 35 min were used.
Biological activities of Brassica rapa (Turnip) callus extracts by plant cell culture technology
Shin, Su Young ; Moh, Sang Hyun ; Hwang, You Jin ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 248~254
DOI : 10.5010/JPB.2016.43.2.248
The purpose of this study was to investigate biological activities of Brassica rapa (Turnip) plant callus extracts of Ganghwa-gun of Incheon city using water, ultrasonic wave and ethanol extractions to develop functional materials. DPPH radical scavenging activities of the callus extracts were increased in a concentration-dependent manner, as compared with control. The astringent effects of the ethanol extracts were higher, as compared to water and ultrasonic extracts. In the collagen synthesis assay, the ethanol extract showed significant anti-wrinkle effects of 59% and 78% at a concentration of 5 ppm and 10 ppm, respectively. These results suggested that water, ultrasonic wave and ethanol extracts of turnip plant calluses are natural antioxidant sources. Especially, the ethanol extract can be regarded as a functional, natural cosmetic material with astringent and anti-wrinkle effects.
In vitro shoot regeneration and genetic transformation of the gerbera (Gerbera hybrida Hort.) cultivar `Gold Eye`
Chung, Mi-Young ; Kim, Min Bae ; Chung, Yong Mo ; Nou, Ill-Sup ; Kim, Chang Kil ;
Journal of Plant Biotechnology, volume 43, issue 2, 2016, Pages 255~260
DOI : 10.5010/JPB.2016.43.2.255
This research was conducted to improve the cold tolerance of the gerbera cv. Gold Eye by introduction of the Arabidopsis
antiporter gene (CAX1) via Agrobacterium-mediated transformation. Prior to genetic transformation, we optimized a combination of plant growth regulators;
6-Benzyladenine (BA) and
3-indole-acetic acid (IAA) were found to lead to proper in vitro shoot regeneration from petiole explants. In addition,
kanamycin was determined to be the minimal concentration useful for selection of putative transgenic plants. In this study, transgenic gerbera expressing the Arabidopsis
antiporter gene (CAX1) were obtained using the optimized concentrations. We expect that introduction of the gene to the cultivar will improve cold tolerance, which will be important in the winter months.