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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY
Journal Basic Information
Journal DOI :
Korean Academy of Pediatric Dentistry
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Volume & Issues
Volume 27, Issue 4 - Nov 2000
Volume 27, Issue 3 - Aug 2000
Volume 27, Issue 2 - May 2000
Volume 27, Issue 1 - Feb 2000
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FACTORS AFFECTING THE FORMATION OF SOLUBLE GLUCAN BY LACTOCOCCUS LACTIS 1370
Oh, In-Gyun ; Yang, Kyu-Ho ; Chung, Jin ; Oh, Jong-Suk ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 185~191
The water-soluble glucan produced by Lactococcus lactis 1370 affects the formation of dental plaque by Streptococcus mutans. In this study the factors affecting the formation of water-soluble glucan were assessed as the optical density of culture supernatant of Lactococcus lactis 1370 in the spectrophotometer. 1. The optical density of culture supernatant was high when Lactococcus lactis 1370 was cultured in M17 broth with 5% sucrose, while being low in culture supernatant of Streptococcus mutans. 2. The optical density of culture supernatant was higher when Lactococcus lactis 1370 was cultured in M17 broth with 10% sucrose than when being cultured without sucrose (p<0.05), and was higher at pH 7 than pH 5 (p<0.05). 3. The optical density of culture supernatant was the highest at
, and was higher in the anaerobic incubator than in the aerobic incubator (p<0.05). 4. The optical density of culture supernatant was the highest in the media containing 1.0mM
(p<0.05), 2.5mM KCl (p<0.05), and 1.6mM
. 5. When Streptococcus mutans was cultured in the media containing a quarter culture supernatant of Lactococcus lactis 1370 grown in M17 broth, the mean weight of produced artificial plaque was 103.0mg on the wire, whereas being significantly reduced to 5.6mg in the media containing a quarter culture supernatant of Lactococcus lactis 1370 grown in M17 broth containing 5% sucrose (p<0.05). These results indicate that the water-soluble glucan is more formed by Lactococcus lactis 1370 in the media containing sucrose or under the adequate conditions for the growth of bacteria, and inhibits the formation of artificial plaque by Streptococcus mutans.
ORAL HEALTH CARE STATUS IN INSTITUTIONS AND SPECIAL SCHOOLS FOR THE DISABLED PEOPLE IN KOREA
Ha, Sun-Yeong ; Lee, Kwang-Hee ; Kim, Dae-Eop ; Park, Jong-Seok ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 192~201
The purpose of study was to investigate the oral health care status in institutions and special schools for the disabled in Korea. Nurses in 123 institutions and nurse-teachers in 102 special schools were asked to fill questionnaires regarding periodic oral examination, preventive dental programs. and dental treatment. The results of this study were as follow. 1. 56.8% of institutions implemented regular oral examinations and all of special schools did it twice a year. 2. Preventive programs for dental caries were done in 69.1% of the institutions and 86.3% of the special schools. Programs included regular toothbrushing and dental health education. 3. Dental treatment was done in 84.2% of the institutions and 39.2% of the special schools. Institutions utilized private dental clinics(60.2%), public health centers(16.8%), volunteers(15.3%), and dental hospitals. 4. 17.7% of the institutions experienced the refusal to treat the disabled by private dental clinics.
COMPARISON OF CARIES EXPERIENCE BETWEEN THE MENIALLY RETARDED AND THE NORMAL
Maeng, Jun-Nam ; Lee, Kwang-Hee ; Kim, Dae-Eop ; Bae, Sang-Man ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 202~207
The purpose of study was to compare the caries experience between the mentally retarded(MR) and the normal(N) children and adolescents and to survey the dental visit frequency. preventive dental care, and tooth-brushing behavior of the mentally retarded. Two hundred and fifty MR and two hundred and ten N subjects were selected for the study. 1. The number of teeth which had experienced caries was 3.28 in MR and 4.66 in N. The number of decayed teeth was 2.24 in MR and 1.50 in N. The number of filled teeth was 0.71 in MR and 3.06 in N Filled teeth rate was 19.78% in MR and 58.89% in N The differences between MR and N were all significant(P<0.01). 2. Dental visit frequency of MR was 1.15 per year, and the 51.2% of MR did not visit the dental clinic for the last one year. 38.0% of MR had received the preventive dental care. 3. Toothbrushing frequency per day was 2.16 and the toothbrushing time for 78.3% of MR was less than two minutes. 51.2% of MR brushed their teeth by themselves, 13.3% did with the help of the carers, and 35.5% totally depended on the carers. In conclusion, MR experienced less dental caries than N, had more untreated caries than N, and had need for regular preventive dental care.
DENIAL TREATMENT OF THE CHILD WITH CONGENITAL HEART DISEASE
Kim, Jae-Gon ; Lee, Yong-Hee ; Kim, Mi-Ra ; Baik, Byeong-Ju ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 208~215
Patient with congenital heart disease are susceptible to infective endocarditis, and bacteremia following dental procedures may lead to infective endocarditis is these patients. Therefore prophylactic antibiotics are recommended for patients with congenital heart disease who are undergoing dental procedures that are associated with infective endocarditis. In 1997 American Heart Association revised guidelines for a prophylaxis against infective endocarditis. The new American Heart Association recommendations for the prevention of infective endocarditis represent a substantial departure from past guidelines. Major change involve the indications for prophylaxis, antibiotic choice and dosing that may reduce bacteremic risk. Previously, antibiotic prophylaxis was suggested for dental procedures associated with any amount of bleeding. Now only those that are associated with significant bleeding are recommended for prophylaxis as dictated by clinical judgement. Recommended antibiotic prophylaxis regimens now consist of a single preprocedural dose, no second dose is recommended. This report presents three cases of dental treatment of patients with congenital heart disease under the most recent American Heart Association recommendations for antibiotic prophylaxis.
SURGICAL EXCISION OF MUCOUS RETENTION PHENOMENON
Kim, Jae-Gon ; Kim, Young-Jin ; Kim, Mi-Ra ; Baik, Byeong-Ju ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 216~221
Mucoceles and Ranulas are mucous retention phenomena, ie, they develop from the extravasation or retention of mucous after trauma to the sublingual gland or one of the minor salivary glands. Mucoceles are chronic in nature, and local surgical excision is necessary. To minimize the chance for recurrence, the underlying feeder glands should be removed in continuity with the mucocele. Ranula is a term used for mucoceles that occur in the floor of the mouth Treatment consists of marsupialization and/or removal of the feeding sublingual gland. Marsupialization entails removal of the roof of the intraoral lesion. However this procedure is often unsuccessful. Some prefer initially to excise the entire sublingual gland. This case report presents two cases. one case was developed on lower lip and treated by marsupialization. There was no recurrence during follow up period.
CLINICAL MANAGEMENT OF ECTODERMAL DYSPLASIA : A CASE REPORT
Oh, So-Hee ; Kwon, Soon-Won ; Kim, Jong-Soo ; Kim, Yong-Kee ; Lim, Hun-Song ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 222~228
Ectodermal dysplasia is a hereditary disease characterized by a congenital dysplasia of one or more ectodermal structures and their accessory appendages. At least 120 subtypes of ectodermal dysplasia have been reported. The disease is usually transmitted as an X-linked recessive trait in which the gene is carried by the female and manifested in the male. Hypohidrotic ectodermal dysplasia is manifested as a triad of defects that include hypohidrosis, hypotrichosis and hypodontia. The characteristic facial features consist of asteatosis, onchodysplasia, sparse and fine blond hair, prominent forehead, a depressed nasal bridge, thick everted lips. The patient may suffer from dry skin, hyperthermia and unexplained high fever as a result of the deficiency of sweat glands. This case report presents detailed procedures of rehabilitating functional and esthetic defect of a 6-year-old boy with hypohidrotic ectodermal dysplasia along with the review of relevant literatures.
A STUDY ON THE MICROLEAKAGE OF COMPOMER RESTORATIONS IN CERVICAL CAVITIES OF PRIMARY MOLARS ACCORDING TO THE LENGTH OF ETCHING TIME
Kim, Sung-Ki ; Jeong, Tae-Sung ; Kim, Shin ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 229~236
For the purpose of evaluating the microleakage of compomer restorations in cervical cavities of primary molars according to the length of etching time, an experiment was performed through next procedures. 48 extracted human primary molars were divided into 6 groups according to applied compomers and the etching time of 0, 15, 30 seconds. All the restorations were filled under the manufacturer's direction, and the degree of dye penetration was evaluated with stereomicroscope. The microleakage of etch group was measured and statistically analyzed and obtained the results as follows: 1. Compared with groups without etching, the degree of microleakage was reduced by acid etching the cavities (p<0.05). 2. There revealed similar preventive effects of microleakage when the teeth were etched for 15 seconds and 30 seconds respectively(p>0.05). 3. No significant difference was observed between two compomers examined in this study(p>0.05). It was, therefore, thought acid etching is effective to minimize the microleakage of compomer restorations in primary teeth, and in cases of performing, 15 seconds is adequate for etching.
A STUDY ON THE RELATIVE SHEAR BOND STRENGTHS OF SOME ADHESIVE RESTORATIVE MATERIALS TO PRIMARY ENAMEL AND DENTIN
Kim, Seung-Mee ; Kim, Shin ; Jeong, Tae-Sung ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 237~245
For the purpose of comparing the bond strengths of some tooth adhesive restoration materials on primary enamel and dentin, 4 kinds (7 brands) of restorative materials including a composite resin (Z 100), a conventional glass ionomer cement (Chem-Flex), 2 brands of resin-modified glass ionomer cements (Fuji II LC-I, Vitremer), and 3 brands of compomers(Dyract AP, F2000, Compoglass) were investigated using UTM for measuring the shear bond strengths. Additionally the failure modes were examined by histologically observing the fractured surfaces of each specimen. The following results were obtained. 1. The shear bond strengths of Z 100 to the primary enamel were higher than those of other experimental materials except Fuji II LC-I, which showed significantly higher bond strength than Chem-Flex or Vitremer (P<0.05). 2. The shear bond strengths of Z 100 to the primary dentin were higher than those of other experimental materials except Dyract AP and Fuji II LC-I, both of which showed significantly higher shear strength than Chem-Flex or Vitremer (P<0.05). 3. The shear bond strengths of all restorative materials except Dyract AP showed relatively higher values to enamel surface than to dentin surface. In Dyract AP, the reverse was true significantly. 4. All materials examined showed cohesive failures except some Chem-Flex and Vitremer, which showed adhesive failures.
APPLICATION OF AN ORAL SCREEN
Park, So-Young ; Jeong, Tae-Sung ; Kim, Shin ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 246~250
The oral screen is a functional appliance, suitable for the treatment of developing malocclusion associated with aberrant muscular patterns. The better muscle balance between tongue and the buccinator mechanism can be established, and the reestablishment of normal growth and development can be achieved. The oral screen can be used for the correction of the following conditions : (1) thumbsucking, tongue thrusting and lip biting, (2) mouth breathing, (3) mild distocclusion with premaxillary protrusion, (4) open bites in deciduous and mixed dentition, and (5) incompetent lips. The patient should wear the oral screen every night and also during the day whenever possible. The effects of oral screen can be elevated through lip seal exercise : the lips should be kept in contact all the time to improve the lip seal. In the presented two cases, the patients were considered mouth breathers and to have incompetent lips, and one patient with maxillary incisal protrusion and the other with open bite. They were instructed to wear the oral screen with lip seal exercise. After wearing the appliance for 1 and 2 years respectively, mouth breathing was decreased and lip length and strength were increased, the maxillary incisors were retruded and open bite reduced.
IDIOPATHIC RETARDED ERUPTION : A CASE REPORT
Ahn, Myung-Ki ; Jeong, Tae-Sung ; Kim, Shin ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 251~255
The strict meaning of the term retarded eruption is that tooth eruption is delayed compared to its developmental stage. The etiological factors for it may be local, systemic or genetic. Its pathogenic mechanism is unknown in most cases. In cases of generalized retarded eruption without any clear reason, the general growth and development seems normal in all aspects and the teeth usually keep the normal eruption sequence. Usually the primary teeth may often be extracted hoping to provoke the eruption of the retarded teeth, only to be proved a failure. Consequently, the patient will be without teeth for many years, with loss of esthetics, loss of occlusal height and resorption of alveolar process. The primary goal in the treatment may therefore be to keep and maintain the primary teeth in good condition until they are naturally shed. This observation was done on 10 year 1 month old patient who were diagnosed as idiopathic retarded eruption through both radiographic and systemic examination and follow-up observation was done for 11 month.
TREATMENT OF CROWN-ROOT FRACTURE BY INTENTIONAL REPLANTATION : CASE REPORT
Son, Ju-Hyo ; Choi, Hyung-Jun ; Sohn, Heung-Kyu ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 256~261
Trauma to the tooth is the most common accidents in pediatric dentistry and tooth fracture occurs frequently. Fracture is classified into crown fracture, root fracture, and crown-root fracture which involves both. Also, it is classified into simple or complicated fracture depending on whether the pulp is exposed or not. When the fracture is extended down to the subgingival level following the long axis of the root, or when more than 1/3 of the root is involved, extraction is the primary treatment. But alternative treatment such as extruding the root fragment with orthodontic force and restoring it, or intentionally extracting the tooth and replanting it to a position which it can be restored. This is a case report on intentional replantation of a traumatized maxillary central incisor with crown-root fracture of a patient with mixed dentition. The teeth was extracted and immediate endodontic treatment, retrograde filling and resin restoration were carried out.
A LONGITUDINAL STUDY OF THE CHILDREN WHO EXPERIENCED NURSING CARIES
Lee, Sang-Eon ; Jung, Tae-Ryun ; Lee, Sang-Hoon ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 262~273
The purpose of this study was to investigate the difference between the children who experienced nursing caries and the children who were caries-free in the prevalence of enamel hypoplasia and DMF rate and to analyse causal factors from questionnaires. Oral examination was performed on 101 children(nursing caries group-45, caries-free group-56)in the 1994 at first, later in 1999. Parents were asked to write questionnaires. As a cotrol group, 50 children were chosen randomly. The results obtained from this study were as follow: 1. The prevalence of enamel hypoplasia of the children who experienced nursing caries was higher significantly than that of the all of the examined children (P<0.01). 2. DMF Tate of the children who experienced nursing caries was higher significantly than that of the children who were caries-free (P<0.01), and higher than that of the all of the examined children also(P<0.05). 3. The children who experienced nursing caries performed tooth brushing less frequently (P<0.05) and started tooth brushing later than the children who were caries-free (P<0.01). 4. The children who experienced nursing caries ate cariogenic food more frequently(P<0.05) and had higher preference(P<0.05) than the children who were caries free. 5. The children who experienced nursing caries showed lower frequency of periodic dental examination than the children who were caries-free or centre) group(P<0.05).
A STUDY OF NI-RESISTANT BACTERIA ON THE RESTORED STAINLESS STEEL CROWN
Chung, Sat-Byul ; Lee, Keung-Ho ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 274~282
Stainless steel crowns are widely used for restoration for primary molars. The material used for the crowns is an alloy of
chromium. Nickel has been known to cause allergic reaction, cancer and cell toxicity. Little is known about nickel with respect to the relationship between Ni-contained SS crown and graining of Ni-resistance in oral microorganisms. The purpose of this study is to examine whether use of Ni-contained SS crown leads to occurrence of Ni-resistant microorganism, especially enterococci. The gingival crevicular fluid of two different groups was taken. Experimental group included patients wearing SS crown, and control group comprised individuals without SS crown. The samples were plated in BHI agar, BHI agar supplemented with nickel chloride at the concentration of 3mM and bile esculin azide (BEA) agar. The cultured enterococci on BEA agar medium were tested their Ni-resistance in nickel-containing media increasing concentrations from 3mM to 50mM. The results were as follows: 1. In experimental group, a total of 507,350 strains were isolated on BHI agar, of which 53,864(10.62%) strains were found to be resistant to 3mM nickel. In control group, of 414,590 isolates on BHI agar, 37,523 isolates were resistant to 3mM nickel. 2. A total of 95 enterococci were isolated on BEA agar in experimental group, while 20 were isolated in control group. of the enterococci, 68 and 12 isolates were found to be nickel-resistant in experimental and control group, respectively. 3. Of 68 nickel-resistant isolates in experimental group, one survived 50mM nickel. In contrast, none of the isolates in control group was observed to grow at the concentrations over 30mM nickel.
COMPARISON OF SEDATIVE EFFECTS BETWEEN THREE ROUTES OF ADMINISTRATION WITH MIDAZOLAM
Kim, Ryoung ; Lee, Keung-Ho ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 283~291
The purpose of this study was to compare the sedative effect of three routes of administration - intramuscular, intranasal drop-in, intranasal spray - with midazolam. The twenty two uncooperative children from 23 months to 76 months who required at least three dental appointment. Each patient was assigned randomly to receive intramuscular(Group I, 0.15mg/kg), intranasal drop-in(Group II, 0.20mg/kg), intranasal spray(Group III, 0.20mg/kg) administration at each visit. Sleep, crying, movement, and overall behavior response were evaluated, and the sedative effects were evaluated by Houpt's rating scale In order to monitor the sedated patients, pulse rate and peripheral oxygen saturation were measured by pulse oximeter during treatment procedures. The results were as follows 1. Pulse rate and peripheral oxygen saturation were stable through all the treatment procedures, and there were no statistically significant differences among three routes of administration(P>0.05). 2. The effect on sleep was, III, II, I, in order, III group was the most effective through all the treatment procedures, except rubber-dam placement and filling phase (P<0.0001). 3. The effects on crying, movement, overall behavior were II, III, I, in order, II group was the most effective through all treatment procedures(P<0.0001).
A STUDY OF PARENTAL KNOWLEDGE AND ATTITUDE ABOUT INFANT ORAL HEALTH CARE
Kim, Myoung-Jin ; Shun, Ye-Kyung ; Shim, Youn-Soo ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 292~299
The purpose of this study was to investigate parental knowledge and attitude and practices relating to infant oral health care and to promote their motivations to their children's oral health care. Authors interviewed 350 persons in Ilwon-Dong and whose children were between 0 to 36 months old and acquired questionnaire from them. The questionnaire constituted of questions about general knowledge of tooth eruption, methods of oral hygiene care, and opinions about preventive dental visit. The collected data were analysed and compared with other researches. The important results obtained from this study were : 1. Females and highly educated persons had more dental knowledge than males and poorly educated persons. But age and economic status did not affect their level of dental knowledge. 2. Fifty nine percents of respondents thought that tooth began to be formed before birth. 3. Seventy six percents of respondents thought that infant oral health care should begin before tooth eruption, but actually only 63% started oral hygiene care before age one. 4. Seventy percents of respondents used gauze for infant oral care. 5. Fifty seven percents of respondents still gave bottles to their children after they reached age one or more and 55% of respondents never heard of nursing caries. 6. Ninety percents of respondents agreed that carious deciduous teeth need restorations, and 31% of respondents thought that age two is the appropriate time for the first dental visit. 7. Forty seven percents of parents did not think that dental caries is an infectious disease. 8. Only Thirty three percents of parents visited dental clinics for examination and prevention during pregnancy and only 11% of expecting parents were offered dental health care education in pediatric or obstetrician clincs. 9. Information about oral health care was mostly given from baby megazines. 10. Overall parental knowledge about infant oral health care was not sufficient to maintain appropriate dental care for infants.
Effect of basic fibroblast growth factor on osteopontin gene expression
Bae, Won-Su ; Kim, Hyun-Jung ; Ryoo, Hyun-Mo ; Kim, Young-Jin ; Nam, Soon-Hyeun ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 300~308
The Fibroblast growth factors(FGFs) plays an important role in the control of osteogenesis during skeletal development. Especially, FGF-2 is a potent mesodermal inducer during embryogenesis and FGF receptors (FGFRs) messages are strongly expressed in developing bones. In this study, we investigated the effect of bFGF on osteopontin(OPN) gene expression in ST-2 cells and tried to elucidate the mechanism of its stimulatory effects. The obtain results were as follows; The treatment of bFGF(1ng/ml) upregulates OPN, fibronectin mRNA levels and downregulates type I collagen mRNA levels. But, there was no remarkable difference in alkaline phosphatase mRNA levels between two groups. The OPN gene expression increased in a dose-dependent manner up to 10ng/ml and OPN gene began to occur at around 3h with continuous increase up to 24h then decreased to basal level at 48h. 30 minutues pretreatment with cycloheximide (500ng/ml), a protein synthesis inhibitor, prior to addition bFGF resulted in blocking bFGF induced OPN expression. These results suggest that bFGF increased the level of OPN mRNA in a dose and time-dependent manner via the synthesis of certain transcriptional regulatory proteins.
EFFECT OF BISPHOSPHONATE ON OSTEOBLAST DIFFERENTIATION
Lee, In-Soon ; Kim, Hyun-Jung ; Ryoo, Hyun-Mo ; Kim, Young-Jin ; Nam, Soon-Hyeun ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 309~317
Bisphosphonates inhibit bone resorption in vivo and in vitro. Currently proposed mechanism of action of bisphosphonates involves both direct effect on osteoclasts and indirect effect through the mediation of osteoblasts. Recent understanding of molecular mechanism of osteoclastogenesis indicates that osteoclast differentiation is quite tightly regulated by signaling molecules from differentiating osteoblasts. Therefore this investigation was designed to elucidate the effect of bisphosphonate on osteoblast differentation. For this purpose, in vitro effects of etidronate and alendronate on the expression of Cbfa1 a master control gene of osteoblast differentiation, several bone marker genes, and formation of calcified nodules were evaluated. To evaluate the effect of bisphosphonate on calcified nodule formation, osteoblasts isolated from rat calvaria were cultured in a-MEM containing
of etidronate or
of alendronate for 15 days, and then stained by alizarin red to determine mineralization. To evaluate the effect of bisphosphonate on osteoblast differentiation, osteoblast cells were cultured in a-MEM containing
of etidronate or
M of alendronate for 8 days. And then total RNA was extracted and northern blot analysis was done to examine the expression of Cbfa1, type I collagen, alkaline phosphatase, osteopontin and osteocalcin. The results were as follows: 1. Etidronate suppressed the calcification of bone nodule in dose dependent manner, while alendronate didn't. 2. The expression of Cbfa1 was decreased dose dependently by etidronate, but increased by alendronate. 3. Etidronate suppressed the expression of type I collagen, osteopontin and osteocalcin in dose dependent manner however alendronate promote the expression of osteoblast marker gene. 4. The expression of alkaline phosphatase was not affected either etidronate nor alendronate. These results suggest that etidronate suppressed the expression of Cbfa1 in dose dependent manner, and consequently the expression of osteoblast marker genes, such as type I collagen, osteopontin and osteocalcin were also suppressed in similar manner. And finally this decreased expression of osteoblastic marker gene prevent calcined bone nodule formation.
A HISTOPATHOLOGIC STUDY ON THE PULPAL RESPONSE TO DEMINERALIZED FREEZE-DRIED BONE IN DOGS
Jung, Moon-Yong ; Lee, Chang-Seop ; Park, Joo-Chul ; Lee, Sang-Ho ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 318~332
The purpose of this study was to investigate the effects of demineralized freeze-dried bone (DFDB) on mechanically exposed pulp of dog by evaluating the pulpal inflammation and healing process, formation of dental hard tissue, and structural changes of fibroblasts of the remaining pulp tissue. Teeth of 4 dogs, weighing 10kg, were used in this study. Class V cavities were prepared followed by exposed the pulp tissue mechanically by sterilized round bur. In control group, exposed pulps were capped with calcium hydroxide paste followed by sealed with IRM. In experimental groups, the exposed pulps of one group were capped with the collagen and those of the other group were capped with DFDB. All cavities were sealed with same manor as control group. The animals were sacrificed at the intervals of 3, 7, 14, and 28 days for histopathlogic evaluation. The specimens were observed by the light microscope and trans-electron microscope. The results were as follows: 1. Pulp necrosis was not observed in all groups. Inflammatory response was disappeared from 1 week in control group and group 2. But it was not disappeared until 2 weeks and also irregular arrangement of odontoblasts was showed at the lateral walls of root canal just beneath the amputated site of the pulp in group 1. 2. Dentinal bridge was formed incompletely at 2 weeks but it was formed completely at 4 weeks in control group. Odontoid tissue was also found in control group at 4 weeks from treatment. Amputated site of pulp was encapsulated with fibrous tissue and odontoblast and dentinal bridge was not found in group 1. Preodontoid tissue and reparative dentin which were formed by odontoblast differentiated around DFDB were found, but dentinal bridge was not found in group 2. 3. Cell with large basophillic-stained nuclei infiltrated to amputated site and DFDB at 1 week from treatment in control group and group 2. They were found more in group 2 than in control group. Odontoblasts arranged more regularly and reparative dentin was found more as time elapsed. 4. Dentin-formative odontoblasts which showed ultramicrostructure of cytoplasm with polarized nucleus, rEM, Golgi complex, secretory granules, secretion of organic matrix in control of group and group 2. In regards to above results, the demineralized freeze-dried bone(DFDB) induce odontoblastic differentiation and further come up to the dentin formation in amputated pulp.
A HISTOPATHOLOGIC STUDY ON THE PULPAL RESPONSE IN DOGS AFTER PULPOTOMY WITH FERRIC SULFATE
Lee, Chang-Seop ; Im, Chul-Seung ; Park, Joo-Chul ; Lee, Sang-Ho ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 333~343
The purpose of this study was to compare the effect of ferric sulfate and formocresol as pulp dressing agents in pulpotomized teeth of dogs. 40 teeth of 5 dogs, weighting 10kg. were used in this study. The animals were sacrificed at the intervals of 3, 7, 14, 28 and 56 days for histopathologic evaluation. The specimens were observed by the light microscope. The results were as follows : 1. Inflammatory response was observed in both groups, but pulp tissue of ferric sulfate group was showed lesser inflammatory degree and more rapid recovery than that of formocresol group. 2. In ferric sulfate group odontoblasts showed irregular arrangement pattern at initial stage and returned to regular pattern after 2 weeks. But in formocresol group. continued irregular pattern of odontoblast was observed during experimental period. 3. Reparative dentin was produced widely along the canal in one specimen of formocresol group at 8 weeks and dentinal bridge was formed in two specimens of ferric sulfate group at 8 weeks.
INTRA-ALVEOLAR TRANSPLANTATION OF COMPLETELY CROWN-ROOT FRACTURED TOOTH WITH DEMINERALIZED FREEZED DRIED BONE GRAFT
Lim, Hyoung-Soo ; Kim, Dong-Phil ; Lee, Chang-Seop ; Lee, Sang-Ho ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 344~350
Incidence of crown-root fracture due to traumatic injury, have been reported 3% in the permanent dentiton, 2% in the deciduous dentition. There are two treatment methods for crown-root fractured teeth with pulp exposure, when the fracture line was located under the alveolar crest. One way is the extrusion by orthodontic force the other way is intra-alveolar transplantation which occlusally repositioning of apical fragment in the alveolar socket. Since intra-alveolar transplantation has introduced in 1970s, it was practiced as alternative to orthodontic extrusion. As the result, this method may thoughted that had a good prognosis. As a result of trauma, completely crown-root fracture was occured in the maxillary right central incisor in this case. We couldn't reposition the deepest fracture line above the alveolar crest by the conventional surgical extrusion, because apical fragment was too short. Thus, after extraction of apical fragment, we repositioned it to the socket following demineralized freezed dried bone graft, which possible to support the apical fragment. At the 15-month recall examination, the root still showed normal mobility and there was not observed any in flammatory or replacement root resorption in the periapical radiograph.
MICROLEAKAGE TEST ACCORDING TO ACID ETCHING TREATMENT IN CLASS V COMPOMERS
Moon, Sang-Hee ; Lee, Chang-Seop ; Lee, Sang-Ho ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 351~360
The purpose of this study was to evaluate the microleakage of class V compomers according to acid etching treatment and treatment times. Extracted 180 sound human molar teeth were selected then prepared physiologic pulpal pressure far this experiment. In this study class V cavities were prepared on buccal surface with gingival margin located in 1mm superior to CEJ under simulate physiological conditions. These specimens were randomly divided into 6 groups of 30 each and restored following methods : A: Dyract AP + Prime&Bond 2.1 Group 1 : No acid etching, according to manufacturer's instruction. Group 2 : 15 seconds acid etching and same method with Group 1. Group 3 : 30 seconds acid etching and same method with Group 1. B: F2000 groups + Single Bond adhesive Group 1 : No acid etching, according to manufacturer's instruction. Group 2 : 15 seconds acid etching and same method with Group 1 Group 3 : 30 seconds acid etching and same method with Group 1. After 500 thermocycling between
, the specimens were sealed with glass ionomer and nail varnish then placed in 5% methylene blue dye for 5 hours and rinsed with tab water. The specimens were embedded in orthodontic clear resin, then sectioned buccolingually through the center of restoration with a low speed diamond saw. The dye penetration on each of the specimens were then observed with a stereomicroscope at
magnification. The results of this study were statistically analyzed using the indepedent sample t-test and analysis of variance. Results were as follows, 1. In occlusal walls, microleakage were significantly reduced in acid etched group restored with Dyract AP but no statistically significance in F2000 groups. 2. In gingival walls, microleakage were significantly reduced in group 2 restored with Dyract AP, and group 2 and group 3 in F2000 groups. 3. All groups, except group 3 in Dyract AP, showed significantly less microleakage in occlusal wall than gingival wall. 4. No statistical significance were showed between group 2 and group 3 in both materials.
INTERACTION OF ORAL ENTEROCOCCUS DURANS WITH STREPTOCOCCUS MUTANS AND STREPTOCOCCUS ORALIS
Kim, Yong-Nam ; Yang, Kyu-Ho ; Oh, Jong-Suk ; Chung, Jin ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 361~369
Enterococcus is a normal inhabitant of the human oral cavity, the vagina, and the gastrointestinal tract. Four isolates of Enterococcus in this study were identified as E. durans. These bacteria were characterized and the interaction of these bacteria with the important oral bacteria as like S. mutans and S. oralis was studied as follows. 1. The carbohydrate fermentation test and biochemical test showed similar results in 4 isolates. 2. The susceptibility test against erythromycin, penicillin, tobramycin, ampicillin, teicoplanin, ciprofloxacin, vancomycin, gentamicin, kanamycin, and streptomycin showed to be susceptible in all four isolates. 3. The optical density of absorbance at 550 nm was 1.405 in the culture of S. mutans in disposable cuvette, whereas being 0.855, 0.867, 0.797, and 1.083 in the combined culture of S. mutans and each E. durans. 4. The mean weight of produced artificial plaque on the wires in the beaker was
in culture of S. mutans only, whereas being reduced to
in the combined culture of S. mutans and each E. durans. The viable cells were
per ml in the culture of S. mutans wheras being
per ml in the combined culture S. mutans and E. durans. 5. The viable cells were
per ml in the culture of S. oralis, wheras being
per ml in the combined culture of S. oralis and E. durans. 6. Plasmid of about 60 kb was isolated in three isolates of E. durans. These results suggested that E. durans isolated from the oral cavity inhibited the replication of S. mutans and formation of artificial plaque, while inhibiting the replication of S. oralis a little.
HYDROLYTIC DEGRADATION OF DENIAL COMPOSITE RESINS
Yang, Kyu-Ho ; Kim, Jung-Ran ;
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY, volume 27, issue 2, 2000, Pages 370~378
The composite restorative resins have their insufficient resistance to wear. The subsurface degradation within the restoration is considered to be associated with wear. The aim of this study was to evaluate the resistance to degradation of six commercial composite resins in an alkaline solution. The brands studied were Clearfil APX(Kuraray), Heliomolar(Vivadent), Surefil(Dentsply), TPH(Dentsply), Tetric Ceram(Vivadent), and Z100(3M). Preweighed discs of each brand were exposed 0.1N NaOH solution at
. After 14 days they were removed, neutralized with HCL, washed with water and dried to constant mass at
. Resistance to degradation was evaluated on the basis of the following parameters: (a) mass loss(%) - determined from pre-and post-exposure specimen weights; (b) Si loss (ppm)-obtained from ICP-AE analysis of solution exposed to specimens; and (c) degradation depth
- measured microscopically (SEM) from polished circular sections of exposed specimens. The results were as follows: 1. The mass loss was in descending order by Z100, TC H, S, CL, TPH and in the range of
2. The degradation layer depth was in descending order by H, Z100, S, TC, TPH, CL and in the range of
3. For the Si concentration, Z100 was the highest of all 4. The highly significant correlation(r=0.81, p<0.05) was observed between mass loss and degradation depth. 5. Under scanning electronmicroscopy, the degradation of connection between resin matrix and fillers was observed 2 weeks after soaking in NaOH solution.