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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Food Science and Technology
Journal Basic Information
Journal DOI :
Korean Society of Food Science and Technology
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Volume & Issues
Volume 45, Issue 6 - Dec 2013
Volume 45, Issue 5 - Oct 2013
Volume 45, Issue 4 - Aug 2013
Volume 45, Issue 3 - Jun 2013
Volume 45, Issue 2 - Apr 2013
Volume 45, Issue 1 - Feb 2013
Selecting the target year
Volatile Aromatic Compounds and Fermentation Properties of Fermented Milk with Buckwheat
Lee, Beom-Seon ; Park, Seung-Kook ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 267~273
DOI : 10.9721/KJFST.2013.45.3.267
We aimed to improve the flavor quality of plain yogurt, which is known to be sour and less desirable in flavor, varying concentrations of a buckwheat saccharification solution (BSS) were added to milk, followed by fermentation with commercially available mixed strains of lactic acid bacteria. Volatile compounds were analyzed using the gas chromatography-headspace-solid phase microextraction (GC-HS-SPME) method. Fermentation properties, including pH, titratable acidity, viable cells, viscosity, and color value were also measured. Eleven volatile compounds were identified with GC-MS. Of which, diacetyl, butanoic acid, and 2-heptanone proportionally increased as the levels of BSS increased. Undesirable compounds such as acetic acid and 2-butanone, decreased as BSS concentration increased. Fermentation properties were significantly altered with the addition of BSS. Our findings indicate that the flavor quality of plain yogurt can be improved by adding BSS for fermentation, with an additional health benefit from buckwheat.
Physicochemical Characteristics of Various Ginseng Seeds
Kim, Hee Jung ; Yoo, Kyung Mi ; Lee, Seul ; Kim, Kyung-Tack ; Hwang, In Kyeong ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 274~278
DOI : 10.9721/KJFST.2013.45.3.274
The aim of our study was to investigate the chemical composition of the Asian ginseng seed (Panax ginseng C.A. Meyer) and the American ginseng seed (Panax quinquifolium L.) grown in Korea (3 years, KGS3; 4 years, KGS4), China (4 years, CGS4), and USA (4 years, AGS4). AGS had the heaviest 100-seed weight (
). The approximate compositions of the ginseng seeds were 13.66-17.00% crude protein, 2.21-8.65% crude ash, 19.06-24.06% crude lipid, and 43.21-47.49% crude fiber. The mineral contents of the ginseng seeds were greater in order of K>P>Ca>Mg>Fe>Na>Zn >Cu. The unsaturated fatty acid content was 96.71-96.94%, and the major fatty acids oleic acid and linoleic acid were present. Total sugar content was 15.00-26.17 mg glucose/g. The acidic polysaccharide content was 0.56-0.80 mg
-Dgalacturonic acid/g. These results showed the differences in the physicochemical characteristics of ginseng seeds with respect to cultivation location, cultivation year, and species.
Classification and Comparative Analysis of the Contents of Acorus species and Anemone altaica by UPLC-PDA Analysis
Jo, Ji Eun ; Lee, A Yeong ; Kim, Hyo Seon ; Moon, Byeong Cheol ; Ji, Yunui ; Chun, Jin Mi ; Kim, Ho Kyoung ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 279~284
DOI : 10.9721/KJFST.2013.45.3.279
A quantitative method using ultra performance liquid chromatography with a photodiode array detector (UPLCPDA) was established for the analysis of 2 major plant metabolites:
-asarone from Acorus gramineus, A. tatarinowii, A. calamus and Anemone altaica, and their contents are compared with other herbs of Acorus species. The method was validated according to the International Conference on harmonization (ICH) guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The average content of
-asarone in Acorus gramineus was significantly higher than that in others, with the second highest concentration observed in A. tatarinowii, and only a trace amounts found in A. calamus and Anemone altaica. In contrast, the average content of
-asarone in A. calamus was the highest, followed by that in Acorus gramineus and A. tatarinowii. principle component analysis (PCA) confirmed that
-asarone content differed among the species. These results suggest that this UPLC-PDA method can be considered as good quality control criteria for Acorus gramineus.
Development and Validation of an Analytical Method for Ametoctradin Residue Determination in Domestic Agricultural Commodities by HPLC-PDA
Do, Jung-Ah ; Kwon, Ji-Eun ; Lee, Eun-Mi ; Kim, Mi-Ra ; Kuk, Ju-Hee ; Cho, Yoon-Jae ; Kang, Il-Hyun ; Kim, Hyung-Su ; Kwon, Kisung ; Oh, Jae-Ho ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 285~292
DOI : 10.9721/KJFST.2013.45.3.285
This study was carried out to validate the safety of ametoctradin residues in agricultural commodities by developing an official analysis method. An analytical method was developed and validated using HPLC-PDA detectors. The samples were extracted with methanol, subsequently partitioned with dichloromethane and purified with florisil column chromatograph using acetone/hexane (30/70, v/v) as solvent. The method was validated by using grape, hulled rice, mandarin, and potato spiked with ametoctradin at 0.05 and 5.0 mg/kg, and pepper at 0.05 and 2.0 mg/kg. Average recoveries were 76-114.8% with relative standard deviation less than 10%, and the limit of detection and limit of quantification were 0.0125 and 0.05 mg/kg, respectively. The result of recoveries and overall coefficient of variation of the laboratory results from Gwangju regional Food and Drug Administration (FDA) and Daejeon regional FDA was accorded with Codex Alimentarius Commission Guideline (CAC/GL 40). Based on these results, this method was found to be appropriate for ametoctradin residue determination and can be used as the official method of analysis.
Physicochemical Characteristics and Antioxidant Activities of Bioresource Juices from Jeju
Lee, Young Jun ; Kim, Dan Bi ; Cho, Ju-Hyun ; Baik, Soon-Ok ; Lee, Ok-Hwan ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 293~298
DOI : 10.9721/KJFST.2013.45.3.293
We aimed to provide the basic data for the development of a beverage using the juices from bioresources from Jeju. Our results show that pH and
of the bioresources ranged 2.0-6.5 and 3.3-16.8, respectively. Rubus coreanus Miquel juice had the highest total phenol content (47.3 mg gallic acid equivalent (GAE)/100 mL). Citrus sphaerocarpa juice showed higher rates of 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging (86.8%) than those of other juices. However, the oxygen radical absorbance capacity (ORAC) value (
TE/mL) of Citrus sudachi Hort. ex Shirai juice was higher than those of other juices. A high correlation (R
Antioxidant Capacity and Bioactive Composition of a Single Serving Size of Regular Coffee Varieties Commercially Available in Korea
Kim, Mi-Jeong ; Park, Ji-Eun ; Lee, Joo-Hyun ; Choi, Na-Rae ; Hong, Myung-Hee ; Pyo, Young-Hee ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 299~304
DOI : 10.9721/KJFST.2013.45.3.299
The major hydrophilic bioactive compounds (chlorogenic acid, caffeine, total phenolics, and flavonoids) and the antioxidant capacity in a single size were evaluated for regular coffee varieties commercially available in Korea. The content of total phenols (63.83-110.12 mg gallic acid equivalents) and flavonoids (35.27-69.27 mg catechin equivalents) were spectrophotometrically determined, and the content of chlorogenic acid (5.17-69.78 mg) and caffeine (74.38-146.32 mg) were determined using HPLC-UV. All varieties of regular coffee studied showed antioxidant potential (88.78-487.52 mg trolox equivalents/serving size), which was conferred by their concentrations of phenolic compounds, caffeine and chlorogenic acid. There were significant (p<0.01) correlations between the total phenolics (
Changes in Quality Characteristics and Chemical Components of Sweet Potatoes Cultivated using Different Methods
Woo, Koan Sik ; Ko, Jee Yeon ; Kim, Hyun Young ; Lee, Yong Hwan ; Jeong, Heon Sang ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 305~311
DOI : 10.9721/KJFST.2013.45.3.305
Effects of cultivation methods on quality characteristics, pasting characteristics, chemical components, and antioxidant activities of sweet potatoes (Ipomoea batatas (L.) Lam) were determined. The Brix degree, hunter color value, pasting characteristics, moisture, protein, and mineral contents of the sweet potatoes showed significant differences from cultivation methods. The total polyphenol and flavonoid contents of the methanolic extracts of the sweet potato`s pericarp were higher than sweet potato`s sarcocarp. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of the sweet potato`s pericarp on the conventional culture and successful cropped hairy vetch culture was 776.38 and 715.20 mg TE/100 g sample. The 2,2`-azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) radical scavenging activity of the sweet potato`s pericarp on the conventional culture and successful cropped hairy vetch culture was 708.03 and 708.58 mg TE/100 g sample. Generally, there was a difference in antioxidant compound content and radical scavenging activity on the methanolic extract of sweet potato with cultivation methods.
Investigation of the Transfer Rate of Zearalenone in Herbal Medicines to Their Decoction
Shim, Won-Bo ; Song, Jeong-Eon ; Kim, Jeong-Sook ; Chung, Young-Chul ; Chung, Duck-Hwa ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 312~316
DOI : 10.9721/KJFST.2013.45.3.312
The objective of this study was to establish an analytical method to detect zearalenone (ZEA) in herbal medicines and their decoctions and investigate the ZEA transfer rate from raw materials of herbal medicines to their decoctions. Herbal medicines (Trichosanthis Semenm, Eucommiae Cortex, Rubi Fructus) spiked with a known concentration of ZEA were presoaked or unsoaked (as a pretreatment) and boiled for 3 h at
or autoclaved for 1 h at
. The decoction and the remnants were separated, cleaned up with an immunoaffinity column, and analyzed using HPLC. Recoveries for decoctions and remnants were 68.39-83.68% and 72.91-80.25%, respectively. ZEA was not detected in the decoction, whereas it was found in the remnants. Although ZEA in the raw material of herbal medicines was not transferred into the decoction during heating and autoclaving, the continuous monitoring for ZEA in raw herbal medicines should be carried out for the safe ingestion and utilization of herbal medicines.
Changes in Physicochemical Characteristics and Antioxidant Activity of Adzuki Bean and Adzuki Bean Tea Depending on the Variety and Roasting Time
Song, Seuk Bo ; Ko, Jee Yeon ; Kim, Jung In ; Lee, Jae Saeng ; Jung, Tae Wook ; Kim, Ki Young ; Kwak, Do Yeon ; Oh, In Seok ; Woo, Koan Sik ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 317~324
DOI : 10.9721/KJFST.2013.45.3.317
This study investigated the changes in physicochemical characteristics of adzuki bean (Vigna angularis var. nipponensis) tea depending on the variety and roasting times, for the development of functional foods. The levels of total polyphenol, flavonoids, and tannin contents were 12.72 mg gallic acid equivalent (GAE)/g, 3.01 mg catechin equivalent (CE)/g, and 3.56 mg tannic acid equivalent (TAE)/g, respectively, for the Jeolgangsung-ipat sample roasted for 16 min. The highest DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was 7.81 mg Trolox equivalent (TE)/g in Bulgeun-ipat sample that was roasted for 14 min. The highest total polyphenol content in the hot water-leached liqueur of adzuki bean tea was
GAE/100 mL in Yungum-pat that was roasted for 12 min. The total flavonoids and tannin contents of Whin-ipat and Jeolgangsung-ipat roasted for 10 min were
CE/100 mL and
TAE/100 mL, respectively. The highest DPPH and ABTS (2,2`-azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid) radical scavenging activities of hot water-leached liqueur of adzuki bean tea were 488.72 and 728.25 mg TE/100 mL, respectively, in Yungumpat roasted that was for 12 min. The results of this study show that roasted adzuki bean tea has notable antioxidant activity and is considered to have significant health benefits.
Choosing Quality Indicators for Quality Prediction of Frozen Green Pumpkin in Distribution
Lee, Hye-Ok ; Lee, Young-Joo ; Kim, Ji-Young ; Kwon, Ki-Hyun ; Cha, Hwan-Soo ; Kim, Byeong-Sam ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 325~332
DOI : 10.9721/KJFST.2013.45.3.325
We aimed to determine the quality indicators for quality prediction of frozen green pumpkin in distribution. Freshly harvested green pumpkin slices were blanched under optimal conditions (confirmed in a preliminary experiment), quick-frozen at
for 24 h, and stored at 0, -5, -15, and
. The correlation between each quality characteristic and the sensory preference rate was analyzed. There was a significant correlation between storage temperature-related drip rate and preference rate across all temperatures, except at
. Hunter values, a and b, showed relatively high correlation coefficient values at -5, -15, and
. Therefore, a change in the Hunter values should be considered when storing green pumpkin, because of their significant correlation with changes in the sensory preference and drip rates. Furthermore, they should be applied as quality indicators for the quality prediction of frozen green pumpkin in distribution.
Amylolytic Activity of Brown Rice and Black Rice during Germination
Lee, Hyang-Mi ; Im, Ji-Soon ; Park, Jong-Dae ; Kum, Jun-Seok ; Lee, Hyun-Yu ; Lee, Young-Tack ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 333~338
DOI : 10.9721/KJFST.2013.45.3.333
Enzymatic activity in germinated cereal grains is important for the saccharification of starch materials. This study was conducted to investigate the amylolytic activities of germinating brown rice and black rice that have different amylose contents. Brown rice and black rice were steeped at room temperature for 24 h and germinated at 20 and
for 1, 2, and 3 days.
-Amylase activities in normal brown rice increased very slightly during the 3-day germination period, but the enzymatic activities were slightly higher in low-amylose (waxy type) brown rice. Diastatic power (DP), a measure of starch-saccharifying enzyme, was higher in the germinating brown rice with low amylose than in those with normal amylose content.
-Amylase activities in black rice increased gradually during germination, and DP of low-amylose black rice appeared to be higher than that of normal brown rice. Amylase activities in brown rice and black rice germinated at
were higher than those germinated at
. Compared to brown rice, the overall amylolytic activity of germinated black rice was observed to be higher than that of brown rice.
Changes in Physicochemical Properties of Paprika by Intense Pulsed Light Treatment
Hong, Hee Joung ; Kim, Ae-Jin ; Park, Hee Ran ; Shin, Jung-Kue ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 339~344
DOI : 10.9721/KJFST.2013.45.3.339
Application of intense pulsed light (IPL) treatment is an emerging technology with interesting prospects in food preservation. However, information concerning the factors affecting the inactivation of microorganisms and their impact on the quality of fresh-cut food is scarce. In this study, the effects of IPL treatment on the microbial inactivation and physicochemical change in paprika were determined. The viability of bacteria in paprika treated with IPL decreased slightly with the treatment time. In addition, water content was slightly decreased after IPL treatment regardless of the color of paprika. However, except in red paprika, sugar content increased after IPL treatment. The pH of paprika increased in all samples, and the polyphenol content decreased with treatment time, but these differences were very small. After IPL treatment of paprika, vitamin C content increased in yellow and red samples. Hunter color values-lightness (L), redness (a), and yellowness (b)-increased in red paprika, but all values decreased in orange paprika.
Physicochemical Characteristics and Functional Components of Mudeungsan Watermelon and the other Cultivars from Korea
Kim, Jeong Yeon ; Lee, Seong Hee ; Hwang, Su Jung ; Kim, Gun Hee ; Eun, Jong-Bang ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 345~349
DOI : 10.9721/KJFST.2013.45.3.345
Physicochemical characteristics of Citrullus lanatus cv. Mudeungsan and 5 other cultivars of Citrullus lanatus were investigated. The color of watermelon (redness) was not significantly different among the cultivars. Hardness ranged from 38.76 to 52.12 force (g) among cultivars. Soluble solid content in Taeyangggul and Mudeungsan were higher than that in other cultivars. pH ranged from 5.39 to 6.02 among cultivars, and total acidity was the highest in Mudeungsan (0.147 mg/100 g). No significant difference in total carotenoid content was observed among the cultivars. Lycopene content in Mudeungsan cultivar was higher than that in other cultivars. Citrullin contents of watermelon flesh and rind in Mudeungsan and GangryeokSambok were higher than those in other cultivars. The major free sugars of watermelon were sucrose and fructose, and the predominant organic acids were succinic acid and citric acid. In conclusion, Mudeungsan cultivar had better properties as outlined above and contained soluble solids and functional components, compared to the other watermelon cultivars.
Characteristics of Fermented Dropwort Extract and Vinegar Using Fermented Dropwort Extract and Its Protective Effects on Oxidative Damage in Rat Glioma C6 Cells
Kim, Min-Ju ; Lee, Sam-Pin ; Choi, Jun-Hyeok ; Kwon, Seung-Hyuk ; Kim, Hyung-Dae ; Bang, Myun-Ho ; Yang, Seun-Ah ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 350~355
DOI : 10.9721/KJFST.2013.45.3.350
The quality of fermented dropwort extract (FDE) and fermented dropwort vinegar (FDV) was assessed for free sugar, organic acid and free and total amino acid content. Major organic acids were lactic acid in FDE and acetic acid in FDV. Free sugars in FDE were fructose and glucose, and those in FDV were fructose, sucrose, and maltose. Aspartic acid was the major free amino acid in both FDE and FDV. Additionally, the main free amino acids in FDE were alanine and
-amino-n-butyric acid (GABA), while those in FDV were arginine and valine. Moreover, to investigate the protective effects of FDE and FDV against oxidative stress induced by t-BHP and
, C6 cells were treated with FDE or FDV prior to inducing the oxidative damage. FDE and FDV inhibited cell death significantly in a dose-dependent manner. These data imply that FDE and FDV may be effective in neuronal cell protection against oxidative damage.
Antioxidant Activity and Inhibitory Effect of Aster scaber Thunb. Extract on Adipocyte Differentiation in 3T3-L1 Cells
Choi, Jun-Hyeok ; Park, Yun-Hee ; Lee, In-Seon ; Lee, Sam-Pin ; Yu, Mi-Hee ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 356~363
DOI : 10.9721/KJFST.2013.45.3.356
Clinical and preclinical trials of involving drugs with anti-obesity effects have focused on screening for herbal medicines suspected to have anti-obesity activities. In this study, an extract of Aster scaver Thunb., which was prepared in 80% methanol (ASE), was assessed for its total phenol content, total flavonoid content, antioxidant activity ability to scavenge the
-picrylhydrazyl, 2,2`-azino-bis-[3-ethylbenzthiazoline]-6-sulfonic acid radical, and anti-adipogenic effects. The anti-adipogenic effect of ASE on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by assaying the suppression of adipocyte differentiation and lipid accumulation by using western blot analysis and the Oil Red-O assay, respectively. The staining results showed that ASE significantly inhibited 3T3-L1. Western blot analysis results showed that ASE decreased the levels of peroxisome proliferator-activated receptor-
, CCAAT/enhancer-binding protein
, and sterol regulatory element-binding protein 1c. These results demonstrate that ASE directly inhibits the differentiation of preadipocytes, and might be an important adjunct in the therapeutic efforts to reduce adipogenesis.
Risk Assessment of Polychrorinated Biphenyls (PCBs) through Food Intake for the Korean Population
Paek, Ockjin ; Suh, Junghyuk ; Park, Heera ; Oh, Keumsoon ; Hong, Selyung ; Lee, Hyunkyung ; Kim, Meehye ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 364~369
DOI : 10.9721/KJFST.2013.45.3.364
Polychlorinated biphenyls (PCBs) have been studied during the past few decades because of their potential impacts on the environment and human health. PCBs are toxic environmental pollutants and persistent organic pollutants (POPs). This study was carried out to assess the dietary exposure and risk to PCBs for the general Korean population through food intake. Various samples (n
Antimicrobial Effects of Propolis against Oral Microorganisms
Kim, Sang-A ; Chung, Hyun-Jung ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 370~375
DOI : 10.9721/KJFST.2013.45.3.370
Propolis is a resinous mixture found in the tree buds, sap flows, and other botanical sources, which is used by honey bees in the construction of their hives. Antimicrobial effects of propolis were evaluated against Streptococcus mutans KCTC 3065, S. sobrinus KCTC 3308, S. sobrinus KCTC 5134, and Porphyromonas gingivalis KCTC 5352 by an agar diffusion assay. Sensitivity of these microorganisms to propolis was evaluated in broth containing different concentrations of propolis at
, followed by observation using transmission electron microscopy (TEM). Propolis inhibited all oral microorganisms tested at the minimum inhibitory concentration (MIC) of
in the agar diffusion assay. Treatment with 0.06 and
of propolis had a bactericidal effect in a concentration- and treatment time-dependent manner against the tested microorganisms. TEM of propolis-treated S. mutans KCTC 3065 and P. gingivalis KCTC 5352 revealed structural damage of the cell membrane. The activity of propolis was affected by heat and pH treatment. The results indicate that propolis shows antibacterial activity against oral microorganisms and that it has potential for future applications in the food industry.
A Study on Total Mercury and Methylmercury in Commercial Tuna, Billfish, and Deep-sea Fish in Seoul Metropolitan City
Kim, Jin-Ah ; Yuk, Dong-Hyun ; Park, Young-Ae ; Choi, Hee-Jin ; Kim, Youn-Cheon ; Kim, Moo-Sang ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 376~381
DOI : 10.9721/KJFST.2013.45.3.376
In this study, we monitored total mercury and methylmercury concentrations in tuna, billfish, and deep-sea fish distributed in Seoul city. With the acquired data, we carried out statistical analysis and an exposure assessment for intake. The mean concentrations (mg/kg) of total mercury and methylmercury were
for patagonian toothfish,
for billfish and
for sharks, respectively. We found that sharks, billfish, patagonian toothfish, and tuna were more contaminated with total mercury and methylmercury, in sequence, and that 66% of the total mercury concentration consisted of methylmercury, on average. Although the estimated weekly intakes of methylmercury from commercial deep-sea fish were lower than the weekly intakes recommended by the Joint FAO/WHO Expert Committee on Food Additives (JECFA), we identified that the total mercury and methylmercury concentrations in tuna, billfish, and deep-sea fish continued to increase with the passage of time. Therefore, we confirmed the necessity of continuous monitoring and comprehensive analysis for general safety.
Monitoring of Uniconazole by Using GC-ECD and GC-MSD
Kim, Eun-Jeung ; Hong, Chae-Kyu ; Choi, Su-Jeong ; Lee, Yun-Jeong ; Hwang, In-Sook ; Kim, Moo-Sang ; Chae, Young-Zoo ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 382~384
DOI : 10.9721/KJFST.2013.45.3.382
We evaluated the presence of uniconazole residual pesticide in agricultural products by using multiclass pesticide multiresidue methods. Samples were collected from January to August, 2012. The pesticide was detected in 14 samples among the 3,632 samples tested. Amount of the uniconazole pesticide ranged from 0.098 to 2.2 mg/kg in the 14 samples. This method was described for the simultaneous determination of uniconazole by using gas chromatography with an electron capture detector (GC-ECD) and mass spectrometry (MS). For evaluating the GC-ECD method, uniconazole was spiked into gyeojachae at a level of 0.05, 0.5 mg/kg. The recoveries of uniconazole with the GC-ECD method ranged from 98.9-109.4%. The results indicate that our method of simultaneous analysis is applicable to uniconazole analysis.
Effect of Tuna Extracts on Production of Nitric Oxide and Inflammatory Cytokines
Kim, Kwang-Hyuk ; Choi, Myoung Won ; Choi, Hyang Mi ; Lim, Sun-Young ;
Korean Journal of Food Science and Technology, volume 45, issue 3, 2013, Pages 385~390
DOI : 10.9721/KJFST.2013.45.3.385
The effect of tuna extracts on the production of nitric oxide (NO) and cytokines including interleukin-6 (IL-6), tumor necrosis factor-
), and interferon-
), was investigated. All extracts and fractions from tuna significantly reduced NO production induced by lipopolysaccharide (LPS). The acetone+methylene chloride (A+M) extract, n-hexane and 85% aqueous methanol (MeOH) fractions had stronger inhibitory effects among them. The 85% aqueous MeOH fraction at a 10-
concentration significantly decreased LPS-induced IL-6 and TNF-
productions at 6 h of incubation. In the case of LPS-induced IFN-
production, the 85% aqueous MeOH fraction at a 3-
concentration showed significantly higher levels at 48 h of incubation. These results show that the 85% aqueous MeOH fraction inhibited the production of NO and pro-inflammatory cytokines (IL-6, TNF-
), suggesting that this fraction acts as a potent immunomodulator.