Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Food Hygiene and Safety
Journal Basic Information
Journal DOI :
The Korean Society of Food Hygiene and Safety
Editor in Chief :
Volume & Issues
Volume 18, Issue 4 - Dec 2003
Volume 18, Issue 3 - Sep 2003
Volume 18, Issue 2 - Jun 2003
Volume 18, Issue 1 - Mar 2003
Selecting the target year
Serotypes and Biochemical Properties of Escherichia coli Isolated from Seafood Products
Journal of Food Hygiene and Safety, volume 18, issue 1, 2003, Pages 1~5
E. coli could cause a variety of different types of diseases, including diarrhea, urinal infection, peritonitis and infant septicemia. Ninety two E. coli strains (12.4%) were isolated among 742 seafood products in Seoul Garak fishery market from January to December in 2001. These isolates were serotyped as O24, O25, O29, O78, O112, O136, O146, O159, O166 and O168. Most E. coli strains were isolated from molluscs (28.1%), shellfishes (14.5%), fishes (10.4%) and crustaceans (4.7%) in summer. Therefore, we knew that E. coli could be contaminated in various seafood products.
Comparative Evaluation on Qualitative PCR using Different Extraction Methods for Nucleic Acids on Soybean and Corn Processed Foods
Journal of Food Hygiene and Safety, volume 18, issue 1, 2003, Pages 6~13
Various kinds of genetically modified organisms (GMO) and processed foods have been developed during recent years. Genetically modified organisms can be classified into several groups as their development methods. Generally, GMO has three foreign DNA regions such as gene expression adjustment region(Promoter), termination region (terminator) and structure gene. Detection of these regions can be done particularly by polymerase chain reaction (PCR). PCR-based detection can virtually be performed for any GMO within short of time. The most important prerequisite for the application of PCR-based detection is to decide abstraction method of efficient nucleic acids. Specially, in the case of processed food, because nucleic acids of foodstuffs are damaged by heat treatment (sterilization), pressure and fermentation, DNA must be extracted ken the samples prior to PCR analysis. Although many DNA extraction protocols are available, they have rarely been compared in a comprehensive method. In this study low widely used commercial and non-commercial DNA extraction methods-DNeasy
, CTAB, phenol/chloroform system-were compared with respect to the quality and yield of nucleic acids and insertion genes.nes.
Environmental Microbial Assessment of Food Services at Elementary Schools in Western Gyeongnam Pyovince
Journal of Food Hygiene and Safety, volume 18, issue 1, 2003, Pages 14~24
Two aims of the present study were the evaluation of hygienic, microbial safety on food services of elementary schools in Western Gyeongnam province, and then, the construction of database for the SSOP (Sanitation Standard Operation Procedures) practice. A total of 98 samples were collected from drinking water, waterworks, kitchen utensils, kitchen equipments, employees and cooked foods. Total bacteria was counted and pathogenic bacteria such E. coli, salmonella, yersinia, vibrio parahaemolyticus and staphylococcus were identified based on the biochemical analysis. Following are the results: the number of bacteria showed from 1.0x10
CFU/ mL to 1.0x10
CFU/mL in most samples, which the level exceeds normal range. Over 1.0x10
CFU/ml bacteria were observed from the kimchi in 4 places (B, C, D, E), because cooked food such as kimchi had not been heated. As a rule, the bacterium level in place B was higher than that in any of the other places. E-coli were isolated from kitchen knives (C, E) and Kimchi (E): staphylococci were isolated from drinking water (A), hands (D), refrigerator (E) and apron (E). But, salmonella, vibrio and yersinia were not detected in anywhere. In conclusion, the presence of bacteria and pathogenic agents in school food service was closely related to hygienic practice. For that reason, it is necessary to have more systematic and efficient management in order to enhance the food safety.
Production of Monoclonal Antibody for Listeria spp. p60 Protein Based on iap Gene
Journal of Food Hygiene and Safety, volume 18, issue 1, 2003, Pages 25~29
The p60 protein of Listeria spp. is a Listeria-Genus-specific, major extra-cellular protein, which is used as an indicator protein for the detection of these bacteria from contaminated foods. In this study, p60 protein were recombinantly produced in E. coli and were purified using amylose resin based column chromatography. Purified recombinant-p6O was used to generate monoclonal antibody against native p60. Antibody from hybridoma cell line, 1H4, specificically reacted with native p60 protein isolated from pathogenic Listeria spp. such as L. monocytogenes, L. ivanovii, L. welshimeri II, but did not or relatively weakly reacted with non-pathogenic Listeia species, L. innocua or other bacterial proteins. Antibody from 1H4 was produced using ascites fluid method and it may be useful to develop the Listeria-detection kits based on immunological method.
Sorbic Acid Contents Survey on Ham, Sausage and Dried Meat in Market
Journal of Food Hygiene and Safety, volume 18, issue 1, 2003, Pages 30~32
This study was performed to investigate the contents of nitrites in 450 meat products in Seoul from Jan. to Dec. in 2002. Sorbic acid contents of the samples were determined by Gas Chromatography. 67 samples of 450 (14.9%) were detected in sorbic acid contents. In each meat products, 65,6% (21/32) in dried meats, 17.6% (3/17) in bacon, 11.7% (27/231) in hams, 11.7% (13/111) in sausages, and 11.5% (3/26) in meat cu. The concentration range of Sorbic acid and its average content in each of the meat products are as follows: ND-1.21 g/ Kg and 0.34 g/kg in dried meat, ND-0.84 g/Kg and 0.12 g/Kg in bacon, ND-1.27 g/Kg and 0.074 g/Kg in ham, ND- 0.90 g/Kg and 0.077 g/Kg in sausage, and ND-0.20 g/Kg, 0.015 g/Kg in can meat. Together, these results demonstrated that the processed market meat products must be reinforced to supply for the citizens as safe foods.
Nitrite Contents Survey on Ham, Sausage and Bacon in Market
Journal of Food Hygiene and Safety, volume 18, issue 1, 2003, Pages 33~35
This study was performed to investigate the contents of nitrites in 450 meat products in Seoul from Jan. to Dec. in 2002. Nitrites of the samples were determined by Diazoa method. One sample of 450 (0.22%) were detected over 70 ppm in NO
￣ contents. In samples detected over 10ppm, 45.9% (106/231) in hams, 62.5% (70/112) in sausages, 37.5% (6/16) in bacons, and 12.5% (2/16) in crushed meats. Together, these results demonstrated that the processed market meat products must be reinforced to supply for the citizens as safe foods.
Acidification of Frying Oil Used for Chicken
Journal of Food Hygiene and Safety, volume 18, issue 1, 2003, Pages 36~41
This study was performed to investigate the condition of frying at fried chicken shop, taste and thinking refer to fried chicken in the consumer. The acidification of frying oil was determined as acid value, peroxide value and fatty acid composition. The results were as follows: 1) Soybean oil was used for frying at a fried chicken shop at 170∼18
for 10∼ 15 min and replaced by every thirty cycles. 2) 76.2% of the consumer among answerers far the question thought that the oil of frying could be harmful to health. 3) The AV and POV were increased while chickens were fried after thirty cycles successively, but the values were lower than a standard level of fried-food. 4) The acidification of fried oil was in progress when chickens were fried for ten days by three times a day, but the value was low. 5) The AV of raw chicken was significantly increased as time goes, suggesting that a fresh chicken should be chosen.