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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Food Hygiene and Safety
Journal Basic Information
Journal DOI :
The Korean Society of Food Hygiene and Safety
Editor in Chief :
Volume & Issues
Volume 18, Issue 4 - Dec 2003
Volume 18, Issue 3 - Sep 2003
Volume 18, Issue 2 - Jun 2003
Volume 18, Issue 1 - Mar 2003
Selecting the target year
Survey of Aflatoxin B1 in Domestic Doenjang and Kochujang Determined by Enzyme Linked-Immunosorbent Assay
Bae, Soo-Ick ; Kwak, Bo-Yeon ; Park, Yun-Kyung ; Kim, Young-Ho ; Shon, Dong-Hwa ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 95~100
Competitive direct enzyme-linked immunosorbent assay(cdELISA) of aflatoxin
) in deonjang(Korean-style soybean paste) and kochujang(fermented hot peppersoybean paste) and the level of
in modern or traditional style deonjang and gochujang, produced in Korea, was surveyed by cdELISA. From the standard curve of the cdELISA, the detection limit of
was 0.2 ng/m/. The average recovery of
was 71.5% in the range of 1~100 ng/g after spiking
into deonjang and it means that it could be possible to detect the
in these range by the cdELISA in deonjang. Among the 30 kochujangs tested, no
was detected in kochujangs. Among the 30 deonjangs,
was detected in 6 ones in the range of 1.0~6.0 ng/g. The occurrence of
in deonjang and kochujang tested in this study was less than the Korea Standard and Specification of aflatoxin in foods (10 ppb).
Detection of Pseudomonas aeruginosa with a Label-free Immunosensor from Various Cold Storage Foods
Kim, Nam-Soo ; Park, In-Seon ; Kim, Dong-Kyung ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 101~106
The aim of this study is to develop a label-free immunosensor for microbial detection and to evaluate its applicability to Pseudomonas aeruginosa detection in various food samples. The antibodies used were a polyclonal antiserum from rabbit (polyvalent type) and a monoclonal antibody raised against the flagella of P. aeruginosa. Antibody immobilization was done by a thiolated antibody chemisorption onto one gold electrode of a piezoelectric quartz crystal with a thiol-cleavable, heterobifunctional cross-linker, sulfosuccinimidyl 6-[3-(2-pyridyldithio)propionamido]hexanoate. To the Stomacher-treated samples from various raw and processed foods under cold storage, comprising sirloin, cod and pettitoes, spiking and enrichment culture were done to prepare the model samples, followed by the measurements of the frequency shifts after sample injections. The frequency shifts obtained by the sample matrices themselves were in the range of 52~89 Hz. The injections of the spiked samples caused the frequency shifts of 108~200 Hz, whereas the enriched samples decreased the steady-state resonant frequencies by 162~222 Hz. All sample measurements including baseline stabilization, sample injection and acquisition of the steady-state response were accomplished within 30 min.
Estimation of Contamination Level of Listeria monocytogenes in meat and meat products Using Probability Approaches
Park, Gyung-Jin ; Kim, Sung-Jo ; Shim, Woo-Chang ; Chun, Seok-Jo ; Choi, Eun-Young ; Choi, Weon-Sang ; Hong, Chong-Hae ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 107~112
Probabilistic exposure assessment has been recognized as an important tool in microbial risk assessment, because of obtained the desired results to characterize of variability and uncertainty associated with the microbial hazards. In addition, it will be provided much more actuality information than the point-estimate approaches. In this study, we present methodology using mathematical probability distribution in exposure assessment and estimating of contamination level of Listeria monocytogenes in meat and meat products as a case study. The result of estimation contaminatin level was mean (
percentile) -4.08 Log CFU/g minimum (
percentile) -4.88 Log CFU/g, maximum (
percentile) -3.56 Log CFU/g.
Antimicrobial Effect of Bamboo (Phyllosrachys Bambusoides) Essential Oil on Trichophyton and Pityrosporum
Lee, Sook-Kyung ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 113~117
In order to develop a antimicrobial agent, bamboo oil was extracted by high temperature suction from dired bamboo truk and then antimictobial activities against Trichophyton and Pityrosporum are investigated. 1. Bamboo oil showed the strongest antimicrobial effect on Trichophyton mentagrophytes > Epidermophyton floccusum > Trichophyton rubrun. Bamboo oil showed the strongest antimicrobial activity on Trichophyton mentagrophytes. 2. Bamboo oil showed the strongest antimicrobial effect on Pityroporum ovale 75 > Pityrosporum ovale 77. Bamboo oil showe the strongest antimicrobial activity on Pityroporum ovale 75.3 Antimicrobial effect in 50%-ethanol extracts of bamboo oil are similar to 100%-bamboo oil.
Evaluation of the Efficiency of E. coli O157: H7 Rapid Detection Kit using Immunochromatography
Kwak, Hyo-Sun ; Lee, Dong-Ha ; Moon, Hee-Sook ; Park, Jong-Seok ; Woo, Gun-Jo ; Kim, Chang-Min ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 118~124
For the rapid detection of various pathogenic microorganisms from food sample, various kinds of kits have been developed and commercially available in the markets. With the advantages of speed, accuracy and easiness, the market of these kits has gradually increased for the QC and QA field of food company as well as testing facilities or laboratories. In this study, the characteristics such as the detection limit and the sensitivity of immunochromatographic type of rapid detection kit (Donga Co, Korea, D-kit) for E. coli 0157:H7 developed by monoclonal antibody were examined and also the possibility of application of the kit to food samples was evaluated. The reference kits used for comparison study were Reveal E. coli 0157:H7 (Neogen Co., USA, R-kit) and VIP EHEC kit (Biocontrol Inc., USA, V-kit) occupying major market share. In the detection limit test with the E. coli 0157:H7 reference, both R-kit and D-kit showed a distinct positive reaction in
/ml and weak positive reaction in
/ml, whereas V-kit showed a same reaction in 105/ml. Also, it was identified that the culture treated with heat showed more sensitivity than no heat treated culture. The sensitivity test was conducted against 22 isolates of E. coli 0157:H7, 7 strains of non-O157:H7 verotoxin-producing E. coli, 40 strains of E. coli with different O and H antigen type, and 38 strains of non-E. coli Enterobacteriaceae, and all of the test strains except three were showed exactly three were showed exactly the same reaction against three kinds of the tested kits. All the three kinds of kits showed a positive reaction against E. coli O157:H19, E. coli O148:H18 and Salmonella galinarium. We suppose that there might be a similarity in serological property between these three strains and O157:H7. From the test results, it can be concluded that there is (was) no difference between the D-kit developed in this study and R-kit or V-kit based on the detection limit and sensitivity.
Heavy Metals in Shellfishes Around the South Coast of Korea
Kim, Ji-Hoe ; Lim, Chi-Won ; Kim, Pyoung-Joong ; Park, Jeong-Heum ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 125~132
The levels of heavy metals (total-mercury, cadmium, lead and chromium) in shellfishes, such as oyster (Carassostrea gigas), blue mussel (Mytilus edulis), arkshll (Scapharca broughtonii) and little neck clam (Ruditapes philippinarum), were determined. The samples were collected from 11 stations for oyster and 12 stations for blue mussel in the South Coast of Korea, and little neck clam and arkshell were collected from Koheung and the Kangjin Bay, respectively, during 1999 to 2000. The average concentrations of heavy metal in 4 different species of shellfishes analyzed were ranged from 0.007 to 0.009
for total-mercury; 0.232 to 0.559
for cadmium; 0.107 to 0.220
for lead; and 0.116 to 0.364
for chromium on wet basis. The highest level of total-mercury, lead, cadmium, and chromium in the samples analyzed were all below the quarantine limit of the Korean regulation and guideline established by the U.S.Food and Drug Administration for human consumption. The levels of heavy metals in the samples were negligible, which could be endogenous. It was elucidated that oyster was able to accumulate 2-times more cadmium than blu mussel in this study.
Characteristics of Puffer Fish Poisoning Outbreaks in Korea (1991-2002)
Kim, Ji-Hoe ; Gong, Qing-Li ; Mok, Jong-Soo ; Min, Jin-Gi ; Lee, Tae-Seek ; Park, Jeong-Heum ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 133~138
The data on outbreaks of puffer fish poisoning during 1991 to 2002 in Korea were gathered from press reports, and characterized information collected. During the period, a total of 32 outbreaks of puffer fish poisoning was reported. These outbreaks caused 111 persons to become poisoning, among the cases 30 persons were fatal. The mean case fatality rate was 27.0%, and most of deaths (93.3%) were the male of above 29 years old. Patient number of below 4 persons per a poisoning accident occupied 75.0% of total outbreaks; 65.8% of total cases; and 66.7% of total deaths) was occurred in the months, November through January. Most of puffer fish poisoning (75.0% of total outbreaks; 68.5% of total cases; and 73.3% of total deaths) were found along the south coastal area of Korea, including Busan, Gyeongsangnam-do, Jeollanam-do and Jeju-do. Over than 80% of puffer fish poisoning outbreaks occurred at fishing boat and home where privately made food was cause, and outbreaks in restaurants accounted for 15.6%. Most commonly implicated foods were Guk, boiled soup with puffer fish meat and spices.
Survey on the Foodborne Illness Experience and Awareness of Food Safety Practice Among Korean Consumers
Park, Gyung-Jin ; Chun, Seok-Jo ; Park, Ki-Hwan ; Hong, Chong-Hae ; Kim, Jeong-Weon ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 139~145
The purpose of this study was to investigate the awareness and practice of Korean consumer on food safety. A telephone survey was conducted from 1,040 adults randomly selected from each province and large city of Korea. Therefore, 12.4% of the subjects experienced foodborne illness at least once a year and 0.3% was hospitalized due to the illness. General restaurant (37.2%) and home (21.2%) were the main causative place of foodborne illness, and the most frequently associated foods were meat and meat products (41.7%) and fish and fish products (18.7%). Regarding the causative agent of foodborne illness, the respsondents were aware of Cholera (75.5%), Vibrio gastroenteritis (73%), Shigellosis (65.5%), Bacillary dysentery (65.5%) and Salmonellosis (47.5%) very well; however very few were aware of Listeriosis (9.9%) and brucellosis (8.3%) and ever believed they were not food-related illness. When the survey data were analyzed based on 3 models (Model 1: Knowledge about the pathogens associated food and water, Model 2: The awareness of food safety, Model 3: Attitudes and behavior about foodborne disease prevention and measure) by Multiple regression analysis. The results showed that the awareness of the causative agent of foodborne illness was significantly related with the previous experience of foodborne illness (OR: 1.714) followed by education level (OR: 0.536) and married status (OR: 0.527). The awareness of food safety was significatly related with education level (OR: 0.702). Education (OR: 0.816) and gender (OR:0.650) were the main factors affecting the awareness of the practice to prevent foodborne illness. However, the previous experience of foodborne illness and food safety education, and the awareness of food safety did not show any correlation, suggesting that the experience and awareness of foodborne illness do not affect the real practice of food safety.
Purification and Crystallization of the Recombinant Catalytic Subunit of Pyruvate Dehydrogenase Phosphatase
Kim, Young-Mi ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 146~152
Pyruvate dehydrogenase phosphatase (PDP) is a mitochondrial protein serine/threonine phosphatase that catalyzes the dephosphorylation and concomitant reactivation of the pyruvate dehydrogenase component of the pyruvate dehydrogenase complex (PDC). PDP consists of a catalytic subunit (PDPc, Mr 52,600) and regulatory subunit (PDPr, Mr 95,600). In the presence of
, PDPc binds to the dihydrolipoamide acetyltransferase (E2) component of the pyruvate dehydrogenase complex in proximity to its substrate, the phosphorylated E1 component, thereby increasing the rate of dephosphorylation. PDPc possesses and intrinsic
binding site and a second
site is generated in the presence of E2. Using the unique interaction, highly pure PDPc was produced by the GSH-Sepharose-GST-L2 matrix with a specific activity of approx. 1000 U/mg and a yield of about 80%.
Determination of Urinary Metabolites of Methidathion after Oral Administration and Dermal Application to Rats
Min, Kyung-Jin ; Kim, Hwa-Sun ; Cha, Chun-Geun ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 153~160
This study was performed to determine the urinary metabolites of methidathion in rats. Urine samples were collected for 24 hours in metabolic cages following after oral administration and dermal application of methidation to rats. The urinary metabolites were identified by GC/MS and the excretion time courses of urinary dialkyl phosphate metabolites were analyzed by CG/FPD. The results obtained are summarized as follows: Three dialkyl phosphate metabolites, DMP, DMTP. and DMDTP, were detected in the rat urine. Urinary dialkyl phosphate metabolites were identified on the basis of their mass spectra by GC/MS. The molecular ions of DMP, DMTP,and DMDTP, were identified at m/z 198, and m/z 158, respectively. A comparison of excretion time courses of urinary dialkyl phosphate metabolites between the orally administrated and dermally applicated rats were also established, After oral administration, 79.2% of DMP, 93.9% of DMTP, and 83.0% of DMDTP were excreted into the urine by 12, 24, and 12 hours, respectively. After dermal application, 71.1% of DMP, 82.8% of DMTP 87.7% of DMDTP were excreted into the urine by 24, 48, 48 hours, respectively. Consequently, almost all of the dialkyl phosphates in oral administration were excreted within 48 hours. However, the metabolites in dermal application were excreted up to 168 hours. In the study, three urinary metabolites of methidation, DMP, DMTP and DMDTP, were detected in the rat both after oral administraion and dermal application with methidathion. And the urinary excretion in dermal application was more delayed than that in oral administration. Based on the results, it tis suggested that three urinary dealkyl phosphates, DMP, DMTP, and DMDTP, could be used as the biomarkers of exposure for methidathion.
Effects of Saururus chinensis Baill on Glutathione and Antioxidative Activity Against TCDD-treated Rats
Ha, Bae-Jin ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 161~165
The effects of Saururus chinensis Baill administration on the biochemical parameters of function in liver of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treated rats were investigated. Saururus chinensis Baill (200mg/kg) was administered into rats intraperitoneally for four weeks, seven days after the injection of TCDD(1
). We examined the antioxidative enzymatic activity by measuring the level of AST and ALT in serum and SOD, Catalase, GPx, GSH and GSSG in liver tissue of rats. STT group showed 70.7% of inhibitive effect in AST acrivity compared to TTA group. ALT level of STT group was decreased to the level of NTT group. SOD and Catalase in TTA group were lower than in NTT group, but SOD and Catalase in STT group were increased by 82% and 55.45% respectively compared to TTA group. GSH contents in STT group were 74.20% increased compared to TTA group. GSSG contents in STT group were 61.08% decreased compared to TTA group. These results suggest that Saururus Chinensis Baill has a potent hepathprotective effect against TCDD intoxicated rats.
Effects of Saururus chinensis Baill on Lipid Metabolism Against TCDD Damage
Ha, Bae-Jin ;
Journal of Food Hygiene and Safety, volume 18, issue 3, 2003, Pages 166~170
This study was carried out to investigate the inhibitive effect of Saururus chinenesis Baill (SCB) on lipid peroxidation in Sprague-Dawely rat (SD-rat) accutely exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Saururus chinenesis Baill (200 mg/kg) was administered into rats intraperitoneally for four weeks, seven days after the injection of TCDD (1
g/kg). we examined th lipid parameters by measuring the levels of Total Cholestero, Triglyceride(TG), HDL-Cholestero, and LDL-Cholesterol in serum and Malone Dialdehyde (MDA) in liver tissue of rats. Cholesterol was siginificantly elevated in TCDD-treated abnormal group (TTA). The higher level of HDL-Cholesterol was found in Saururus chinenesis Baill and TCDD administered group (STT), which showed the lower levels of Total-Cholesterol and LDL-Cholesterol. TG content in the TTA was 34.84% increased compared to Non TCDD-treated group (NTT). That of STT was inhibited by 18.90% compared to TTA. MDA content in the TTA was 44.72% increased compared to NTT. That of STT was inhibited by 17.14% compared to TTA.