Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Food Hygiene and Safety
Journal Basic Information
Journal DOI :
The Korean Society of Food Hygiene and Safety
Editor in Chief :
Volume & Issues
Volume 20, Issue 4 - Dec 2005
Volume 20, Issue 3 - Sep 2005
Volume 20, Issue 2 - Jun 2005
Volume 20, Issue 1 - Mar 2005
Selecting the target year
Screening of Korean Medicinal Herbs for Hormonal Activities using Recombinant Yeast Assay and MCF-7 Human Breast Cancer Cells
Yang Se-Ran ; Hong Hee-Do ; Cho Sung-Dae ; Ahn Nam-Shik ; Jung Ji-Won ; Park Joon-Suk ; Jo Eun-Hye ; Hwang Jae-Woong ; Sun bo ; Park Jung-Ran ; Lee Seong-Hun ; Jung Ji-Youn ; Choi Changsun ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 1~6
To investigate whether there are estrogenic and anti-estrogenic activities in various medicinal herbs and discover prominent chemo-preventive agents, we screened and compared the ethanol extracts of 9 plants through the recombinant yeast assay and MCF-7 human breast cancer cell assay, In recombinant yeast assay, seven medicinal herbs showed estrogenicity, and four extracts showed androgenecity. In MCF-7 proliferation assay, the growth of MCF-7 cells was inhibited by eight extracts before and even after co-treatment with bisphenol A. It is interesting that the extracts of Glycyrrhiza uralensis, Cassia tora, Syringa velutina, Zingiber officinale, Malva verticillata, and Panax ginseng C.A. Meyer exhibited inhibitory effects as phytoestrogens in estrogen-responsive human breast cancer cells. This study suggests that some Korean medicinal herbs might be considered as phytoestrogens and be useful to further analyze those plants which contain the estrogenic effect in order to identify the active principles.
Four-Week Repeated Dose Safety Test of Rhus-II Orally Administrated to Rats
Choi Changsun ; Han Dong Un ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 7~12
The purpose of this study was to examine the four week repeated toxicity in Sprague-Dawley rats orally administrated with Rhus-II (water fraction of Rhus Veniciflua). In acute toxicity test, three groups (40 rats of both sex) were administrated different dosages of Rhus-II, 2 g/kg (high dosage group), 1 g/kg, 0.5 g/kg and one group (10 rats of both sex) were received by orally only saline according to the Regulation on Korea Food and Drug Administration, respectively. There was no difference in body weight change, feed intake and water consumption among different dose groups. There was no alteration in relative organ weight by the administration of Rhus-II. No death of abnormal clinical signs was observed during the experimental period. Between the groups orally administered Rhus-II and the control group, there was no statistical significance in urinalysis, hematological test or serum biochemical values. There were no gross findings at final sacrifice. There was no evidence of histopathological alteration mediated by four week treatment with Rhus-II. These results suggest that no observable effect level(NOEL) of the test orally administration was considered to be more than 2g/kg in rats under the conditions employed in this study.
The Observation of the Skin Contact Allergic Sensitization Test of Rhus-II with Guinea Pig Maximization Test
Choi Changsun ; Han Dong Un ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 13~17
The purpose of the present study was to investigate differences in the sensitizing potential of Rhus Veniciflua(Rhus-II), when tested by the guinea pig maximization test(GPMT) and Freund's complete adjuvant test(FCAT) with an identical, intradermal induction concentration. A new grading classification of the sensitization potential is proposed. The GPMT was conducted according to OECD guideline
, using a multiple-dose design and test results were analysed with logistic regression analysis. During the induction stage, we injected intradermally each three site 0.1 ml(l mg/animal) test material, 0.1 ml complete Freund's adjuvant and 0.lml the test agent emulsified in the adjuvant. 7 days later, we induced weak sensitization with
sodium lauryl sulfate(SLS) and applide 1ml(l0mg/animal) test agent topically on the same site and made a tight occlusion. 14 days later we challenged with 1 ml(l 0mg/animal) of test material on the flank and observed ant 24 hours and 48 hours later. The results were also observed
at 24 hours challenge. The results observed 48 hours after challenge were the identical. These data indicated that, although Rhus-II is a no contact allergen. It was reported that the skin sensitization by Rhus-II was not detected the skin sensitization in the guinea pig maximization test (GPMT). Consequently, it was confirmed that Rhus-II had no contact allergic sensitization in guinea pig maximization test.
Genotoxicicological Safety Estimate for the Rhus-II
Choi Changsun ; Han Dong Un ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 18~21
These observations were performed to investigate the safety of the natural herbs (Rhus-II) in respect of genotoxicity. This substance was examined in two in-vitro tests: (1) Salmonella typhimurium reversion assay (Ames test) in strain TA 98, TA 100, TA 1535 and TA 1537, (2) in vitro chromosome aberration test in cultured Chinese hamster ovary (CHO) cells. In the reverse mutation test, Rhus-II did not induced mutagenicity in Salmonella typhimurium reversion assay(Ames test) with or without metabolic activation. In the chromosome aberration assay using CHO cells, there was no increased incidence of structural and numerical aberrations with or without metabolic activation. These results indicated that, the Rhus-II had no genotoxicity.
A Survey on the Perception of Food Sanitation Officers Toward the Genetically Modified Foods
Oh Kyeung Nam ; Lee Soon Ho ; Lee Woo Young ; Park Hye Kyung ; Park Sun Hee ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 22~35
A survey was conducted to investigate the perception of food sanitation officers toward the Genetically Modified Foods. They were mainly from Regional Agencies of KFDA, City/Province office, and National quarantine station. Some of them were professors of university and researchers of research institute. Most of respondents had experiences of hearing or reading GM foods (over
) and over
of respondents much needed the label of GM foods. Although some of officers of city/province office and national quarantine station showed less knowledge than other respondent groups, most of respondents had basic knowledge about biology. The frequency of respondents worked over 20 years and worked in the general administration was higher than that of other groups in the question of unsafe of GM foods. The answer frequency of careless treatment of foods was highest in the question of risk factor, and the frequency of GM foods was lowest (
). It was concluded that food sanitation officers had positive opinion about GM foods, but there were some differences in the knowledge among agencies. Therefore, it is necessary more educations and informations are needed for food sanitation officers.
Microbiological Evaluation for HACCP Guideline of Korean Traditional Cookies
Lee Je Myoung ; Park Jae Young ; Lee Hye Ran ; Lee Mi Seon ; Yoon Sun Young ; Chung Duck Hwa ; Lee Jong Mee ; Oh Sangsuk ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 36~42
The purpose of this study is to develope a HACCP model for Korean traditional cookies. Prerequisite program and HACCP plan were proposed and analyzed for Yugwa. After analysing hazards of raw material and manufacturing processes, CCP, CL and control methods were presented. Pathogenic microorganisms were detected at the Korean traditional cookies plant. It gave a clue that general sanitation control procedures should be implemented for safe traditional cookie products. Hazard analysis of raw materials and processing of Korean traditional cookies, determination of critical control points and critical limits at the plant lead to present a model of HACCP plan. Implementation of HACCP can be carried out using a HACCP model. Critical control point of Yugwa production were determined as frying process. Biological and chemical hazards were presented for CCP determination.
Microbial Contamination Levels of Fresh Vegetables Distributed in Markets
Choi Jin-Won ; Park Shin Young ; Yeon Ji-Hye ; Lee Min Jeong ; Chung Duck Hwa ; Lee Kyu-Ho ; Kim Min-Gon ; Lee Dong-Ha ; Kim Keun-Sung ; Ha Sang-Do ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 43~47
This study monitored and compared the contamination levels of total aerobic bacteria, coliforms, Escherichia coli, and L. monocytogenes of either lettuce, sesame leaf, or cucumber sampled from either 15 super markets(SM) or 21 traditional markets(TM) located in both Seoul and the southern part of Gyunggi. Contamination levels of total aerobic bacteria and coliforms in lettuce, sesame leaf, or cucumber from SM or TM were not (p>0.05) significantly different. The highest contamination levels of total aerobic bacteria were observed in lettuce and followed by sesame leaf and cucumber. The contamination levels of total aerobic bacteria in lettuce, sesame leafs, and cucumbers were
, respectively. A similar pattern of contamination rank was observed with the coliforms in three vegetables as was observed with the total aerobic bacteria E. coli were not significantly (p>0.05) different between SM and TM and isolated over
in lettuce and sesame leaf and below
in cucumbers. L. monocytogenes were not detected in all three vegetables(ND: cucumber <3 CFU/g, lettuce and sesame leaf <10 CFU/g). The microbial contamination levels determined in the present study may be used as the primary data to execute microbial risk assessment of fresh vegetables.
Antimicrobial Activities of Green Tea Extract and Fractions on the E. coli O157:H7
Cho Sun-Young ; Choi Jae-Ho ; Ham Seung-Shi ; Oh Deog-Hwan ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 48~52
This study was investigated to determine the antimicrobial activity of ethanol extract of green tea, and of solvent fractionated ethanol extract on Escherichia coli O157:H7. MIC value of ethanol extracts and solvent fractionated ethanol extract (hexane, ethyl-acetate, chloroform and water) were
, respectively. The antimicrobial activity of ethyl acetate fraction against E. coli O157 :H7 ranged from
. The antimicrobial activity was significantly increased as concentration dependent. When E. coli O157:H7 was pH-adjusted TSB containing
ethyl acetate, E. coli O157:H7 was significantly inhibited at initial pH of 10, whereas the pathogen grew well in the presence of pH 4.5-pH 9. The inhibitory effect of the ethyl acetate fraction on the growth of E. coli O157 :H7 was investigated. Growth of both strain in the tested conditions were rapidly occurred up to 12 h, but no growth was occurred in the presence of
of ethyl acetate fraction for 72 h.
Antioxidative, Antimutagenic and Cytotoxic Effects of the Mineral Water
Ham Seung-shi ; Kim Soo-hyun ; Moon Seon-young ; Jeon Mi-Sun ; Oh Deog-Hwan ; Cui Cheng-Bi ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 53~57
This study was performed to observe the components, antioxidative, antimutagenic and cytotoxic effects of the mineral water using AOAC method, DPPH free radical donating method, Ames test and SRB assay. Mineral water contained eleven kinds of minerals among the total seventeen components and sodium and potassium ion were main components. Mineral water showed electron donating activities (
). The inhibition rate of mineral water (
) in the Sallmonella typhimurium TA98 strain showed
against the mutagenesis induced by Trp-P-1. In addition, same concentration of mineral water the Sallmonella typhimurium TA100 strain showed highest
, 4NQO and Trp-P-1, respectively. The cytotoxic effects of mineral water against the cell lines with Human lung carcinoma (A549), Human breast adenocarcinoma (MCF-7), Human stomach adenocarcinoma (AGS) and Human cervical adenocarcinoma (Hela) were inhibited with the increase of the mineral water. The treatment of
of mineral water showed cytotoxicities of
against A549, MCF-7, AGS and Hela.
Micronucleus Test of Wild Ginseng Culture Extract Using the Marrow Cells in ICR Mice
Song Si-Whan ; Yang Deok Chun ; Choung Se Young ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 58~63
To assess clastogenic effects of the wild ginseng culture extract (WGCE) in vivo micronucleus test was performed using 7 weeks old ICR mice. At 24 hours after 2nd treatment with wild ginseng culture extract at the doses of 0, 500, 1,000, and 2,000 mg/kg/day by peritoneal route mice were sacrified and marrow cells were prepared for smear slides. As a result of counting the micronucleated polychromatic erythrocyte (MNPCE) of 2,000 polychromatic erythrocyte(PCE), all treatment groups did not show statistically significant increase than negative control group. And there was no clinical sign connected with injection of wild ginseng culture extract. It was concluded that wild ginseng culture extract did not induce micronucleus in the marrow cells of ICR mice.
Comparison of General Test Methods of Various Organs on Synthetic Food Colors
Shin Dong-Hwa ; Kim Yong-Suk ; Lee Young-Hwan ; Bang Jeong-Ho ; Om Ae-Son ; Shin Jae-Wook ; Lee Tal-Soo ; Hong Ki-Hyoung ; Park Sung-Kwan ; Choi Duck-Jang ; Kim Hee-Yun ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 64~68
An analytical method of nine synthetic foods colors in Korea, Japan, Joint FAO/WHO Expert Committee of Food Additives (JECFA), and USA were compared. Contents of water insoluble matter in synthetic food colors tested were not different by general test methods of various organs. Contents of chloride and sulfate salts in Food Green No.3, Food Red No.3, Food Blue No.2, and Food Yellow No.4 slightly differed in various methods, and but up to the standard, and general test methods of JECFA and USA needed far more time. For the measurement of arsenic contents in food colors, colorimetric method in Korea and Japan, and silver diethyldithiocarbamate colorimetric method in USA and JECFA were used, but the standards of them were different. Content of heavy metals was up to the standard, but the methods were used colorimetric method in Korea, atomic absorption method in Japan, and both methods in JECFA and USA.
Effect of Mixed Glucomannan and WheyCalcium on the Serum Cholesterol and Blood Glucose in Rats
Lee Sook Kyung ; Sin Gang Lae ; Kim Young Han ;
Journal of Food Hygiene and Safety, volume 20, issue 1, 2005, Pages 69~72
This study was performed to investigate the effect of mixed glucomannan(G) and wheycalcium(W) on the level of serum cholesterol in rats(Female Sprague-Dawley) fed high cholesterol diet(
: HCD) and also on the level of blood glucose in rats fed high glucose diet (
: HGD) for 4 weeks. The results were follows as 1. The level of serum cholesterol were decreased in the rank order HCD>HCDG>HCDGW>ND, 2. HCDG showed a decrese of about
but HCDGW showed a decrese of about
compared with the increasing rate of HCD in serum cholesterol, 3. The level of blood glucose were decreased in the rank order HGD>HGDG>HGDGW>ND, 4. HGDG showed a decrese of about
but HGDGW showed a decrese of about
compared with the increasing rate of HGD in blood glucose, 5. The mixture of glucomannan and wheycalcium on the serum cholesterol and blood glucose in rats was most effect of all.