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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Food Hygiene and Safety
Journal Basic Information
Journal DOI :
The Korean Society of Food Hygiene and Safety
Editor in Chief :
Volume & Issues
Volume 22, Issue 4 - Dec 2007
Volume 22, Issue 3 - Sep 2007
Volume 22, Issue 2 - Jun 2007
Volume 22, Issue 1 - Mar 2007
Selecting the target year
Verification of Mold Determination Method using Slide Culture by Monitoring
Lee, Hee-Sook ; Park, Kun-Sang ; Sin, Yeong-Min ; Lee, Myung-Ja ; Lim, Jong-Mi ; You, Hyun-Jeong ; Kim, Ki-Hyun ; Cho, Dae-Hyun ; Kim, Dai-Byung ; Kim, Ok-Hee ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 137~144
Koji is steamed rice that has had koji-kin, or koji mold spores, cultivated onto it. The isolation, culture, and microscopic examination of molds in the koji require the use of the selective media and special microscopic slide techniques. If simple wet mount slides of molds were attempted, it became apparent that wet mount slides made from mold colonies usually don't reveal the arrangement of spores that is so necessary in identification. The process of merely transferring hyphae to a slide breaks up the hyphae and sporangiophores in such a way that identification becomes very difficult. The slide culture method is superior to wet mounts in that the hyphae, sporangiophores, and spores remain more or less intact when transferred. The procedure that will be used to produce a mold culture on a slide that can be observed directly on the slide. We investigated the contamination rate of penicillium spp. on the 21 kinds of koji distributed at Korea. The contamination rate of Penicillium spp. were not detected at 21 products by slide culture method. These results will be used to reestablish a mold determination of koji and food in Food Additives Code.
Comparison of Diglyceride, Conjugated Linoleic Acid, and Diglyceride-Conjugated Linoleic Acid on Proliferation and Differentiation of 3T3-L1 Preadipocytes
Jeong, Jae-Hwang ; Lee, Sang-Hwa ; Hue, Jin-Joo ; Lee, Yea-Eun ; Lee, Young-Ho ; Hong, Soon-Ki ; Jeong, Seong-Woon ; Nam, Sang-Yoon ; Yun, Young-Won ; Lee, Beom-Jun ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 145~150
Conjugated linoleic acid (CLA) reduces fat deposition in several mammalian species. The proposed mechanisms for this effect are reduced preadipocyte proliferation and differentiation. The objective of this study was to investigate the inhibitory effects of diglyceride (DG), CLA, DG-CLA of proliferation and differentiation of 3T3-L1 preadipocytes. Cell viability was determined using WST-8 analysis and cell differentiation was determined by glycerol-3-phosphate dehydrogenase (GPDH) activity. Lipid accumulation in differentiating 3T3-L1 cells was measured by Oil red O staining. The proliferation of preconfluent 3T3-L1 cells by treatments of DG, CLA, and DG-CLA was reduced in a dose-dependent manner. CLA among them was the most effective in reduction of viable cells with increasing concentrations. Treatments of the DG, CLA, and DG-CLA at the concentration of
for 48h significantly inhibited differentiation of 3T3-L1 cells (p<0.05). In addition. cytoplasmic lipid accumulation during differentiation of the 3T3-L1 preadipocytes was also inhibited by treatments of the test solutions. DG-CLA was the most effective in the inhibition of differentiation and lipid accumulation in 3T3-L1 cells. These results indicate that the DG including CLA as fatty acids is more effective for anti-obesity than DG or CLA alone and that consumption of DG-CLA as a dietary oil may give a benefit for controlling overweight in humans.
Bactericidal Effect of Bacteriocin of Lactobacillus plantarum K11 Isolated from Dongchimi on Escherichia coli O157
Lim, Sung-Mee ; Im, Dong-Soon ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 151~158
Among 68 strains of lactic acid bacteria (LAB) isolated from Dongchimi, a strain K11 was selected due to its bactericidal activity against Escherichia coli O157 The strain K11 was identified as Lactobacillus plantarum, based on physiological and biochemical characteristics. In the late exponential phase, La. plantarum K11 showed maximum bacteriocin activity (12,800 BU/mL) and maintained until the early stationary phase. The bacteriocin activity was completely inactivated by all the proteolytic enzymes such as pepsin, protease, proteinase K, papain, chymotrypsin, and trypsin, but the activity was not affected by catalase, a-amylase, lysozyme, and lipase, suggesting proteinaceous nature of the bacteriocin. Additionally, this activity was not affected in the pH range from 3.0 to 9.0 and under storage conditions like 30 days at -20,4, or
. Although the bacteriocin activity was absolutely lost after 15 min treatment at 121, it was relatively stable at
for 60 min or
for 30 min. The activity was disappeared by treatment with acetone, benzene, ethanol, or methanol, but it was not affected by treatment with chloroform or hexane. The antibacterial activity of the bacteriocin was good against some LAB including Lactobacillus spp., Enterococcus spp., and Streptococcus spp., but not against food-borne pathogens such as Bacillus spp., Listeria spp., and Staphylococcus spp. as well as yeasts and molds. Especially, some intestinal bacteria such as Enterobacter aerogenes and E. coli were significantly affected by the bacteriocin of La, plantarum K11. Furthermore, the addition of 640 BU/mL resulted in the complete clearance of E. coli O157 after 10 hr.
Hygienic Quality of Drinking Water Served in Restaurants and Quality Characteristics of Drinking Water Boiled with Various Plant Materials
Ahan, Yu-Bok ; Jeong, Eun-Ju ; Lee, Shin-Ho ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 159~164
The viable cell of 60% drinking water among 25 drinking water served in restaurant of Daegu district showed above 100 CFU/mL which is sanitary standard of drinking water. The 50 CEO of restaurant considered drinking water served in their own restaurant as just safe and were very deficient in aware of sanitation about drinking water. Sensory quality of drinking water boiled with plant materials, such as barley, Cassia semen, corn, Buckwheat, Korean solomon's seal, Chamomile, Brown rice-green and peppermint was improved in color, taste, flavor and overall acceptability compare to marketed drinking water, respectively. Especially drinking water boiled with Korean solomon's seal was the best In overall acceptability. The number of total bacteria reached above
in marketed drinking water after 4 days storage at 10. But the growth of microorganism did not occur in boiled drinking water with Brown rice-green tea, Korean solomon's seal, barley, Cassia semen and com during storage for 10 days at
, respectively. The number of total bacteria reached above
in marketed drinking water after storage for 1 days at
. The growth of microorganism was accelerated in boiled drinking water with corn, Korean solomon's seal, barley, Cassia semen except Brown rice-green tea during storage far 10 days at
A Survey on the Sanitary Management with Step-by-step Working Process in Food Service Institutions
Shin, Dong-Hwa ; Soh, Gowan-Soon ; Kim, Hyeong-Eun ; Kim, Yong-Suk ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 165~172
Sanitary management with step-by-step working process and dietician's perception against them at 98 food service institutions located in Jeollabuk-Do were surveyed. Food service institutions included 13 hospitals, 38 schools,40 enterprises, and 7 others. Practice ratio of sanitary management items at purchasing and receiving step of food and food materials in food service institutions were 59.2-98.0%, and they were higher than those of other steps. In pre-treatment step, practice ratio of 'Undoing treatment of foods on the ground' in hospital was higher (53.8%) than those of enterprise (32.5%) and school (34.2%), and needed the improvement of pre-treatment procedure in enterprise and school. Practice ratio of all sanitary management items in cooking step were below 30%, and needed the improvement of cooking procedure. In storage step, the improvement of cooking procedure in school and enterprise were needed. Practice ratio of all sanitary management items in distribution step were low, and needed the improvement of this working procedure. However, in spite of low practice ratio on these items, dietician's perception against sanitary management items in all steps was low. Therefore, we estimated that the improvement of working processes and the conversion of dietician' perception on sanitary managements in food service institutions were needed.
Survey of Caffeine levels in the Favorite Diets of Children
Lee, E-Na ; Kim, Hee-Jin ; Im, Ji-Young ; Kim, Jeoung-A ; Park, Hye-Young ; Ryu, Ju-Young ; Ko, Kwang-Rack ; Kim, Hyung-Sik ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 173~178
Children may respond differently to the caffeine from adults because they have different physiologic makeup and are functionally immature in terms of hepatic and renal function; this leads to the slower clearance of caffeine in early life. Therefore, children are often assumed to be more susceptible to caffeine effects. Alarge number of food supplements may interfere with these processes, and therefore caffeine exposure may have more serious consequences for children than for adults, irrespective of sensitivity. However, there has never been a national dietary survey on caffeine intakes in children. The purpose of our study was to identify caffeine intakes and beverage sources of caffeine in a representative sample of children in Busan, Korea. Caffeine intakes were based only on beverages included in the Continuing Surveys of Food Intakes by individuals. The caffeine content of the beverages ranged from 2.8 to 65.2mg/100ml for cola, soft drinks, and teas. Caffeine was not completely absent from caffeine-free colas, juice, and milk. In this study, cola-type beverages were an important dietary source of caffeine in the children. Daily caffeine intake for children was estimated to range from 12.5 to 250 mg/day. In general, the acceptable daily intake (ADI) of caffeine should cover the entire population including children. Therefore, special considerations should be needed regarding the consumption of soft drinks containing caffeine to children below the 12 years of age.
Survey of the level of Microbial Contamination in Fish Farms on the Jeju-Island
Moon, Young-Gun ; Ha, Jin-Hwan ; Kang, Chang-Hee ; Song, Chung-Bok ; Oh, Myung-Cheol ; Heo, Moon-Soo ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 179~191
The purpose of this study was to monitor and compare the contamination levels of heterotrophic bacteria and pathogenic bacteria (total coliforms, Escherichia coli O157:H7, Salmonella spp., Vibrio parachaemolyticus, Staphylococcus aureus and Bacillus cereus). The samples collected at fish farms located in the Jeju-island were investigated from March to August in 2006. The contamination levels of S. aureus, B. cereus and Salmonella spp. ranged
, respectively, and 85% of moist pellet feeds were contaminated with S. aureus, B. cereus and total coliforms. But total coliforms and E. coli O157:H7 were not detected in oliver flounder (Paralichthys olivaceus). For the production of safe oliver flounder (Paralichthys olivaceus), education of sanitation for employees, control of fish feed, and continuous monitoring for microorganism will be required.
Comparative Evaluation of Washing Methods of Chinese Cabbages for Eliminating the Parasite Eggs in the Preparing Kimchi
Choi, In-Uk ; Youn, Young-Nam ; Yu, Yong-Man ; Choi, Min-Ho ; Lee, Young-Ha ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 192~198
Kimchi is one of the representative traditional foods in Korea, which is recognized as a kind of wellbeing foodstuffs. However, as increasing foreign-made Kimchi, its safety as food is at the forefront of public health concerns. We analyzed the washing methods of Chinese cabbages, which are the main materials in Kimchi, to prevent parasite contamination during preparing it. To decontaminate parasite eggs from Chinese cabbages, discard the discolored outer leaves from cabbages, spread the space of leaves with fingers and rinse more than 3 separate water tanks with streaming water (velocity more than 0.8m/sec). At each tank, Chinese cabbages were rinsed with more than 3 strokes upward and downward within the streaming water followed by moving back and forth more than 3 times, with 20cm in height and 30cm in width, respectively. Decontamination efficiency increased higher in parallel with streaming velocity of water, and with adding the vegetable detergent to the tank water.
The Degradation Patterns of Three Pesticides in Perilla Leaf by Cultivation, Storage and Washing
Seo, Jung-Mi ; Kim, Jong-Pil ; Yang, Yong-Shik ; Oh, Mu-Sul ; Chung, Jae-Keun ; Shin, Hyeon-Wo ; Kim, Seon-Ju ; Kim, Eun-Sun ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 199~208
Three pesticides commonly used in perilla leaf were subjected to a field residue trial to ensure safety of terminal residues in the harvest. The residual patterns of three pesticides, which were dimethomorph, indoxacarb and procymidone were examined after applying with the recommended and double dose and their DT50 were calculated. Also degradation patterns of pesticides at storage
were compared to those at
, and removal rates of pesticides by washing perilla leaf with water were measured. Biological half-lives of dimethomorph, indoxacarb and procymidone were
days, respectively. During the storage period, the degradation patterns were appeared more obviously at
. Removal rates of dimethomorph, indoxacarb and procymidone were
by various washing methods.
The Estrogenic Effects of Phthalates (DEHP, DBP) in MCF-7 Cell
Lee, Su-Youn ; Kim, So-Jung ; Lee, Seung-Ho ; Park, Young-Seok ; Park, Byung-Kwon ; Kim, Byeong-Soo ; Kim, Sang-Ki ; Choi, Chang-Sun ; Yoon, Seong-Il ; Kim, Jong-Suk ; Jung, Ji-Won ; Jung, Ji-Youn ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 209~212
To evaluate the estrogenic activities of di-ethyl hexyl phthalate (DEHP) and di-butyl phthalate (DBP), two phthalates known as endocrine disrupters, we used MCF-7 human breast cancer cell line. As results, DBP and DEHP had estrogenic effects. In brief, the concentration of maximal MCF-7 cell proliferation was
for DEHP and DBP, respectively. The ratio of maximal cell yield of the test compounds to that of
was 87.5% for DEHP and 73.4% for DBP. In summary, both DEHP and DBP had cell proliferation potencies in the MCF-7 cell. Potencies ranged from approximately 10 to 100 times less than 17beta-estradiol. DBP was stronger than DEHP in the concentration of maximal efficacy. However, DEHP was stronger than DBP in the MCF-7 cell proliferation. Results from this study suggested that DEHP and DBP may play an important role in the estrogenic activity. Therefore, it is suggested that DEHP and DBP are estrogenic.
The Effect of Rubus coreanum Miquel Against Lipopolysaccharide-induced Oxidative Stress and Lipid Metabolism
Kim, In-Deok ; Kang, Kum-Suk ; Kwon, Ryun-Hee ; Yang, Jeong-Ok ; Lee, Joong-Sook ; Ha, Bae-Jin ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 213~217
LPS induces the synthesis of several inflammatory cytokine, chemokine, NO and inflammation in the liver of rats. The purpose of this study was to investigate the preventive effects of Rubus coreanum Miquel (RCM) In lipid metabolism. RCM of 100 mg/kg concentration was intraperitoneally administered into rats at dose of 1.5ml/kg for 20 days. On the day 21, 1.5ml/kg of LPS was injected 4 hours before anesthetization. We examined the lipid-related functions by measuring the levels of triglyceride (TG), total cholesterol (TC), total lipid (TL), high-density lipoprotein cholesterol (HDL-C) in serum and malondialdehyde(MDA) in liver tissue. The results showed that LPS treatment increased the values of TG, TC, TL and MDA, decreasing that of HDL-C. But RCM pretreatment decreased the high values of TG, TC, TL and MDA to the low values and increased the low value of HDL-C to the high value. These results suggested that RCM could be used as the potential candidate for the lipid metabolism natural supplement.
The Estrogenic Effects of Phthalates(DEHP, DBP) in Yeast Recombinant Assay
Jung, Ji-Youn ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 218~222
Di(2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) were screened for estrogenic activity using a recombinant yeast screening system consisted with estrogen receptors and
as reporter gene. The chemicals showed estrogenic activity in ranges of
, as a positive control of, showed maximal activity at
. The concentration of half-maximal estrogenic activity was
for both chemicals. However, the concentration of maximal estrogenic activity was
for DEHP and
M for DBP. These results suggested that DBP was higher in relative potencies and more sensitive than DEHP. In conclusion, DEHP and DBP were both estrogenic, even though DBP was more reactive to estrogen receptor.
Comparison of Antiplatelet Activities of Green Tea Catechins
Cho, Mi-Ra ; Jin, Yong-Ri ; Lee, Jung-Jin ; Lim, Yong ; Kim, Tack-Joong ; Oh, Ki-Wan ; Yoo, Hwan-Soo ; Yun, Yeo-Pyo ;
Journal of Food Hygiene and Safety, volume 22, issue 3, 2007, Pages 223~230
We have previously reported that green tea catechins(GTC) displayed potent antithrombotic effect, which was due to the antiplatelet activity. In the present study, the antiplatelet activity of each green tea catechin components was compared in vitro. Galloylated catechins including (-)-epigallocatechin gallate (EGCG), (-)-gallocatechin gallate (GCG), (-)-epicatechin gallate (ECG) and (-)-catechin gallate (CG), significantly inhibited collagen
rabbit platelet aggregation with
values of 79.8, 63.0, 168.2 and
, respectively. EGCC GCG and CG also significantly inhibited arachidonic acid (AA,
)-induced rabbit platelet aggregation with
values of 98.9, 200.0 and
, respectively. However catechins without gallate moiety showed little inhibitory effects against rabbit platelet aggregation induced by collagen or AA compared with galloylated catechins. These observations suggest that the presence of gallate moiety at C-3 position may be essential to the antiplatelet activity of catechins and the presence of B ring galloyl structure may also contribute to the antiplatelet activity of GTC. In line with the inhibition of collagen-induced platelet aggregation, EGCG caused concentration-dependent decreases of cytosolic calcium mobilization, AA liberation and serotonin secretion. In contrast, epigallocatechin (EGC), a structural analogue of EGCG lacking a galloyl group in the 3' position, although slightly inhibited collagen-stimulated cytosolic calcium mobilization, failed to affect other signal transductions as EGCG in activated platelets. Taken together, these observations suggest that the antiplatelet activity of EGCG may be due to inhibition of arachidonic acid liberation and inhibition of
mobilization and that the antiplatelet of EGCG is enhanced by the presence of a gallate moiety esterified at carbon 3 on the C ring.