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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Food Hygiene and Safety
Journal Basic Information
Journal DOI :
The Korean Society of Food Hygiene and Safety
Editor in Chief :
Volume & Issues
Volume 25, Issue 4 - Dec 2010
Volume 25, Issue 3 - Sep 2010
Volume 25, Issue 2 - Jun 2010
Volume 25, Issue 1 - Mar 2010
Selecting the target year
Migration Measurement of Volatile Organic Compounds (VOCs) from Polystyrene-made Food Containers into Distilled Water
Kim, Nam-Hoon ; Kim, Ae-Kyeong ; Cho, Tae-Hee ; Park, Kyung-Ai ; Kwak, Jae-Eun ; Kim, Ji-Young ; Kim, Il-Young ; Chae, Young-Joo ; Kim, Min-Young ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 203~208
In this study, the level of migration of 5 kinds of volatile organic compounds (VOCs) (toluene, styrene, ethylbenzene, isopropylbenzene and n-propylbenzene) into distilled water from polystyrene-made food containers was measured using Purge&Trap combined with GC/FID. The contents of the VOCs which have regulatory limits in Korea food code only for material specification were determined under three exposure conditions which were 30 min at
, 30 min at
and actual situation of instant noodle intake. The calibration curve of 5 compounds showed good linearity (
= 0.9976~0.9995) within the concentration range of 1~50 ng/mL. The limit of detection (LOD) and limit of quantification (LOQ) were validated at range of 0.041~0.092 and 0.135~0.304 ng/mL, respectively. The average migration contents of 5 compounds were below 5 ng/mL except for styrene. The average contents of styrene were highly detected at
for 30 min exposure (52.71 ng/mL). Under actual condition at instant noodle intake, the average contents of styrene was 17.23 ng/mL. The results demonstrated that the migration rate of VOCs was related to storage temperature and time.
Brazilin Inhibits of TPA-induced MMP-9 Expression Via the Suppression of NF-
Activation in MCF-7 Human Breast Carcinoma Cells
Kim, Byeong-Soo ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 209~214
Metastasis is the primary cause of from breast cancer mortality. Cell migration and invasion play important roles in neoplastic metastasis. Matrix metalloproteinase-9 (MMP-9), which degrades the extracellular matrix (ECM), plays an important role in cancer cell invasion. NF-
is transcription factor important in the regulation of MMP-9, as the promoter of MMP-9 gene contains binding sites for NF-
. Brazilin, an active component of sappan wood (Caesalpinia sappan), decreases TPA-induced MMP-9 expression and invasion in MCF-7 cells. Also, brazilin suppressed NF-
activation in TPA-treated MCF-7 cells. Taken together, we demonstrated that the inhibition of TPA-induced MMP-9 expression and cell invasion by brazilin is mediated by the suppression of the NF-
pathway in MCF-7 cells. This result suggest brazilin provide a potential therapeutic app roach for the treatment of breast cancer.
Antimutagenic Study on Acanthopanax Koreanum Nakai
Cho, Myung-Chan ; Hong, Chang-Eui ; Lyu, Su-Yun ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 215~219
This study was undertaken to investigate the mutagenicity and antimutagenicity of Acanthopanax koreanum Nakai. Antimutagenic study on extract of A. koreanum was studied using the test with Salmonella typhimurium TA100, TA98. And mutagenicity study was studied using the test with S. typhimurium TA100, TA98, TA1535, TA1537 and Escherichia coli WP2 uvr A. A. koreanum was negative in Ames test with S. typhimurium and E. coli with or without S-9 mixture. Test substances of
of A. koreanum extracts were chosen via toxicity test. Ames test was performed on positive control group, experimental group and negative control group in the presence of the metabolic activation system and metabolic non-activation system. As a result, there was no coherent increase and reverse mutation in all concentrations. Therefore, A. koreanum does not cause reverse mutation. In addition, A. koreanum showed strong antimutagenic activities in S. typhimurim TA100 and TA98. In conclusion, A. koreanum root may be an excellent antimutagenic agent.
Antimicrobial Resistance and Implicated Genes of E. coli Isolated from Commercial and Cooked Foods in Seoul
Yoo, Young-Ah ; Kim, Moo-Sang ; Kim, Kyong-Sik ; Park, Sun-Hee ; Jung, Sung-Kuk ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 220~225
Distribution of foodborne E. coli strains, antimicrobial resistant genes and antimicrobial susceptibility have been carried out on E. coli isolated from commercial and cooked foods distributed food in Seoul. Of total 1,313 samples, fifty samples(3.8%) were found E. coli that included one of the ETEC and EPEC, respectively. The serotype of ETEC in seasoning raw meat was E. coli O26 and produced Verotoxin 2. Fifty percentage of total isolates were susceptible to all antimicrobial agents. Specially, there were ampicillin(36%), amoxicillin/clavulanic acid(32%) and tetracycline(22%) etc. Resistant gene (tetB) were found in four tetracycline resistant E. coli strains, and TEM gene was found in one ampicillin resistant E. coli isolate.
Analysis of Medroxyprogesterone Acetate in Meats
Lee, Yoon-Ae ; Shim, Jee-Youn ; Lee, Ryun-Kyung ; Kim, Seung-Hwan ; Oh, Hyun-Sook ; Cho, Tae-Yong ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 226~231
We aimed at the monitoring medroxyprogesterone acetate (MPA) residue amount in meats and confirmed the safety of its residue in meats. Optimized condition for analytical and instrumental methods was obtained by method validation. The limit of detection (LOD) and limit of quantification (LOQ) were validated at 1.5 and 5.0 ug/kg, respectively. The calibration curve showed good linearity (
= 0.9968) within the concentration range of 5.0~50.0 ug/kg. We selected progesterone-d9 for internal standard, The recoveries in fortified meat ranged from 67.5 to 109.56% at the 3 spiking levels. As the regulation of MPA analysis method used by LC-MS/MS on other products have established. We selected 3 species of farm stock products (cattle, pig, chicken) and purchased at the markets of seven major cities. The total 196 of meat including 46 of domestic beef, 43 of import beef, 60 of domestic pork, 12 of import pork and 35 of domestic chicken. No residue of synthetic growth hormones were detected in cattle, pig and chicken samples tested.
Safety Assessment of Oriental Medicines and Their Preparations
Jeong, Il-Hyung ; Kim, Jong-Hwa ; Jeon, Jong-Sup ; Cho, Sang-Hun ; Park, Shin-Hee ; Jo, Hyun-Ye ; Kim, Young-Sug ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 232~237
This study was carried out to evaluate the safety of circulated oriental medicines and their related preparations in Gyeonggi-do. Total 366 samples (165 species) were analyzed about heavy metals, residual contents of sulfur dioxide (
), and 68 samples were analyzed about preservatives. 17 samples (13 species, 4.6%) were exceeded the legal limit of heavy metal. The concentrations over the legal limit for Pb, Cd, As and Hg were 6.1~19.2 mg/kg, 0.4~0.7 mg/kg, 6.9 mg/kg and 0.7 mg/kg, respectively. In particular, the exceeding ratio (5.9%) of the legal limit of heavy metals in foreign products was 3.3 times more than domestic products (1.8%). 13 samples (10 species, 3.6%) exceeded the limit of residual sulfur dioxide and the concentration ranges were 105 to 428 mg/kg in domestic products, on the other hand foreign products were from 114 to 2,468 mg/kg. The mean concentration over the limit of residual sulfur dioxide of foreign products (804 mg/kg) was 2.4 times more than domestic products (338 mg/kg). In studying of the preservatives in oriental preparation, the contents of dehydroacetic acid (48.9~64.1%) in 3 samples of labeled solutions were under the labeled preservative contents and the contents of benzoic acid (139.9%) in 1 sample of labeled pill product was exceeded the labeled preservative contents. The numbers of the detected preservatives in unlabeled solutions, pills and granules were 4, 11 and 7, respectively.
Comparison Study on Efficacies of Disinfectants and Sanitizers Among Methods for Quantitative Surface Test
Kim, Ae-Young ; Kim, Yong-Su ; Ha, Sang-Do ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 238~244
Currently, in vitro suspension tests using tubes are used as a authorized test method for sanitizers and disinfectants. However, the methods could not accurately assess the efficacy of sanitizers and disinfectant on the food-contacted surfaces in the field. This study evaluated the effectiveness of 5 kinds of representative sanitizers and disinfectants against E. coli and S. aureus to compare three quantitative surface testing methods that have been internationally standardized. As a result, the ASTM E2111-05 (ASTM(1)) test method obtained 5.18
0.03 and 5.27
0.04 log cfu/carrier reduction in dealing with E. coli and S. aureus, respectively, the ASTM E2197-02 (ASTM(2)) test method obtained 4.63
0.04 and 3.97
0.03 log cfu/carrier reduction and the CEN EN 13697 test method should 6.14
0.05 and 5.31
0.10 log cfu/carrier reduction in clean condition (CEN(1)) but 4.37
0.02 and 4.06
0.01 log cfu/carrier reduction in dirty condition (CEN(2)). Among them, CEN(1) showed the highest bactericidal effects, whereas ASTM(2) and CEN(2) revealed low performance (p < 0.05). In conclusion, the bactericidal effects of the ASTM(2) method and the CEN EN 13697 method adopting stainless steel were lower than the ASTM(1) method, which uses glass. The effectiveness assessment results among nationally accredited test methods were different each other. This implies that they could not fit for in the accurate evaluation of sanitization and disinfection on food-contact surfaces in practical food-processing fields. These results could be used as a basic data for establishment of an official surface test methods applicable in the field.
Residue Levels of Pesticides in Post-Harvest Treated Import Fruits During Storage
Hwang, Lae-Hwong ; Cho, Tae-Hee ; Cho, In-Soon ; Eom, Jeung-Hoon ; Choe, Bu-Chuhl ; Park, Young-Hye ; Kim, Hyun-Jeong ; Kim, Jung-Hun ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 245~250
The post-harvest concentration change of four kind of pesticides, captan, chlorpyrifos, methidathion and kresoxim-methyl was investigated with the storage period for the import fruit. Then the post-harvest concentration was set to 1000, 250, 400 and 157
for captan, chlorpyrifos, methidathion and kresoxim-methyl respectively. In case of captan, 0.9-12.5
in total fruit base and ND-0.23
in the sarcocarp were measured after atomization and 0.7-3.2
in total fruit base and ND-0.67
in the sarcocarp were measured after four week. For chlopyrifos, menthidathion, and kresoxim-methyl 0.4-2.2, 0.7-3.1 and 1.3-2.1
in total fruit base and ND-0.32, ND-0.05 and ND-0.16
in the sarcocarp were measured after atomization respectively. After four week 0.3-0.9, 0.4-2.0 and 1.3-1.8
in total fruit base and ND-0.02, ND-0.05 and ND-0.15
in the sarcocarp were investigated for other three pesticides. The concentration decreasing ratio of pesticides was largest for captan, 52% and other components were in order of chlopyrifos, menthidathion, and kresoxim-methy, 47, 41, 11% each other.
Quality Determination of Different Wholesale Cuts of Goat Carcass at Different Ages
Islam, R. ; Rahman, S.M.E. ; Khan, M. ; Akhter, S. ; Hossain, M.M. ; Ding, Tian ; Kim, Jai-Moung ; Oh, Deog-Hwan ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 251~257
The experiment was conducted to find out the nutritive value and microbial status of different wholesale cuts of goat carcass. The meat sample (4 cuts from each animal
3 different age groups
3 animals in each group = 36 samples) was obtained from 1-, 2-, and 3-year aged goats. The whole sale cuts were shoulder, rack, loin and leg of each goat carcass. To assess the quality of meat sample, the general appearance, color, smell, juiciness, proximate composition, pH, total bacteria, coliform bacteria, and yeast were studied. The mean pH value of different cuts ranges from 5.65-5.69 didn't differ significantly, but due to age differences the pH values (5.59-5.74) differed significantly (p < 0.01). The values of juiciness in different ages ranged from 32.24-42.10% which differed significantly (p < 0.01). The marbling of the cuts of rack portion was more pronounced than that of other cuts. The ranges of crude protein (CP) content of goat carcass (20.78-27.71%) differed significantly (p < 0.01) and leg portion contained higher CP than other portion. Fat contents of different cuts ranged from 2.66-11.47% differed significantly (P < 0.01). The moisture content of the carcass differed significantly which ranged from 69.20-73.31%. The ash content of the cuts of 1-year aged groups (0.99
0.13%) was higher than that of other age groups and differed significantly (P < 0.01). The calcium (Ca) content did not differ significantly. The phosphorus (P) content was higher in one year old goat (0.15
0.03%) than that of the goats of other ages. The total viable count (TVC) content of microorganisms ranging from 5.05-5.15 log cfu/g at different ages did not differ significantly. The coliform count (CC) of different cuts differed significantly (P < 0.01) which ranged from 2.56-3.05 log cfu/g; it also differed significantly (P < 0.05) in different ages (2.79-2.84 log cfu/g) and was higher in 1 year old goat carcass. The yeast count differed significantly in different cuts (P < 0.01) and ages (P < 0.05). From the study it is concluded that the age and different wholesale cuts have direct influence on quality of goat carcass.
Role of p53-dependent PI3K in Radioresistance of Colon Cancer Cells
Lee, Heui-Kwan ; Kim, Jong-Suk ; Kwon, Hyoung-Cheol ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 258~262
Radiotherapy is one of the major therapies for cancer treatment. p53 acts as a central mediator of the cellular response to stressful stimuli, such as radiation. Recently it has been known that activation of the phosphatidylinositol-3-kinase (PI3K) pathway is associated with radioresistance. In this study, we investigated whether X-irradiation up-regulates PI3K in a p53-dependent manner in human colon cancer cells. In order to study this phenomenon, we have treated p53-wild type and p53-mutant type HCT116 cells with X-ray. Treatment of wild type HCT116 cells with 8 Gy resulted in a marked increase in PI3K (p85), which paralleled an increase in PTEN, a counterpart of PI3K. However, these effects of X-rays in the p53-mutant cells were not observed. These results suggest that the X-irradiation-induced up-regulation of PI3K/PTEN pathway is p53-dependent.
Toxicity Study of Detoxication Sulphur at 3 Months Post-treatment in Rats
Lee, Jin-Seok ; Kwon, Jung-Ki ; Han, Sang-Hyeon ; An, In-Jung ; Kim, So-Jung ; Lee, Seung-Ho ; Park, Young-Seok ; Park, Byung-Kwon ; Kim, Byeong-Soo ; Kim, Sang-Ki ; Kim, Il-Ho ; Choi, Chang-Sun ; Jung, Ji-Youn ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 263~268
This study was demonstrate a repeated oral dose toxicity of detoxication sulphur in 8-weeks-old female Sprague-Dawley (SD) rats. The rats were treated with dose of 0.2%, 1%, 5% detoxication sulphur and 1% sulphur of feed consumption administered for 13 weeks. To evaluate the safety of detoxication sulfur, we examined the body weight, the feed intake, the clinical signs, the ophthalmological test, the hematological and the serum biochemical analysis. We also observed the histopathological changes of liver and kidney in rats. As a result, no significant differences in body weight, feed intake, hematological examination and histopathological between control and detoxication sulphur treatment group were found. Serum biochemical results were not shown significant differences in 0.2% and 1% the treated groups compared with control group. But glucose level were decrease, also ALT and ALP level were increase in 5% treated group. All of these results indicate that 1% detoxication sulphur of feed consumption may be safety in SD rat.
Effect of Dietary Selenium on the Colon Carcinogenesis in Male ICR Mice
Cho, Min-Haeng ; Kim, Jun-Hyeong ; Hue, Jin-Joo ; Kang, Bong-Su ; Park, Hyun-Ji ; Nam, Sang-Yoon ; Yun, Young-Won ; Kim, Jong-Soo ; Jeong, Jae-Hwang ; Lee, Beom-Jun ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 269~277
Selenium is an essential micronutrient for normal body function and functions as an essential constituent of selenoproteins. This study was carried out to investigate effect of selenium on the formation of colonic aberrant crypt foci (ACF) and tumor formation in a mouse model. Five-week old ICR mice were acclimated for one week and fed different selenium diet (0.02, 0.1, and 0.5 ppm) for 12 weeks. Animals received three intraperitoneal injections of azoxymethane (10 mg/kg B.W. in saline for 3 weeks), followed by 2% dextran sodium sulfate in the drinking water for a week. There were four experimental groups, including a normal control group and three different selenium levels groups. After sacrifice, the total numbers of aberrant crypt (AC) and ACF were measured in the colonic mucosa after methylene blue staining. The number of tumors was noted for tumor incidence. Liver selenium concentration was measured using ICP-AES method. Gutathione peroxidase (GPx) activity was determined using a GPx assay kit in the liver and colon. TUNEL assay and proliferating cell nuclear antigen (PCNA) staining were performed to examine the cell apoptosis and cell proliferation, respectively. Immunohistochemistry of
-catenin was also performed on the mucous membrane tissue of colon. The activity of GPx in the liver and colon was decreased in the selenium-deficient diet group while it was increased in the selenium-overloaded diet group. Apoptotic positive cells were increased in the selenium-overloaded diet group but decreased in the selenium-deficient diet group. PCNA staining area was decreased in the selenium-overloaded diet group. In addition, the
-catenin protein level in the selenium-deficient diet group was increased but decreased in the selenium-overloaded diet group. These results indicate that dietary selenium might exert a modulating effect on colon cancer by inhibiting the development of ACF and colon tumor formation in this mouse model.
Development of a Multiplex Polymerase Chain Reaction Method for Simultaneous Detection of Genetically Modified Soy and Maize
Park, Kyoung-Sik ; Kim, Mi-Gyeong ; Leem, Dong-Gil ; Yoon, Tae-Hyung ; No, Ki-Mi ; Hong, Jin ; Kwon, Eun-Mi ; Moon, Ae-Rie ; Jeong, Ja-Young ;
Journal of Food Hygiene and Safety, volume 25, issue 3, 2010, Pages 278~280
This study was aimed to develop a novel qualitative multiplex polymerase chain reaction (PCR) for simultaneous detection of genetically modified (GM) soy and maize within a single reaction. The specific primers designed to detect four respective GM events (A2704-12, MON88017, Bt11, and MON863) were included in the tetraplex PCR system. Each of PCR products for four GM events could be distinguished by agarose gel based on their different lengths. The specificity and reproducibility of this multiplex PCR were evaluated. This multiplex PCR consistently amplified only a fragment corresponding to a specific inserted gene in each of the four GM events and also amplified all four of the PCR products in the simulated GM mixture. These results indicate that this multiplex PCR method could be an effective qualitative detection method for screening GM soy and maize in a single reaction.