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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Food Hygiene and Safety
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Journal DOI :
The Korean Society of Food Hygiene and Safety
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Volume & Issues
Volume 28, Issue 4 - Dec 2013
Volume 28, Issue 3 - Sep 2013
Volume 28, Issue 2 - Jun 2013
Volume 28, Issue 1 - Mar 2013
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Inhibitory Effects of S-Allylmercaptocysteine Derived from Aged Garlic on Cholesterol Biosynthesis in Hepatocytes
Yang, Seung-Taek ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 89~94
DOI : 10.13103/JFHS.2013.28.2.089
The present study was undertaken to elucidate the mechanisms underlying the cholesterol-lowering effect of S-allylmercaptocysteine (SAMC) derived from aged garlic. Rat hepotocytes and HepG2 cells were used to determine the short-term effects of SAMC on [
] acetate incorporation into cholesterol, and several enzymatic steps. The cells were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and treated with 20, 40, 60 and 80
of SAMC. At concentration of 20~40
, no significant cells viability effect was noted during those incubation periods. However, at a concentration 60
, cell viability decreased approximately 50% compared with the control. The treatment of cells with 5, 10, 15, and 20
of SAMC resulted in a marked of [
]-acetate incorporation into cholesterol. At concentration of 15
, the cholesterol synthesis was inhibited 79% in cells. The activities of lipogenic enzymes, fatty acid synthase (FAS), and glucose-6-phosphate dehydrogenase (G3PDH) were measured in culture hepatocytes treated with the inhibitors. The activity of FAS in cells treated with 0.95 nmol SAMC was 19% lower than that of nontreated cells, and no affected G6PDH activity, 3-hydroxy-3-methylglutaryl Co A activity was decreased at concentration dependant manner. The present study demonstrates that SAMC is effective in inhibiting cholesterol biosynthesis.
Antibacterial Effects of Galla Rhois Extract against Streptococcus suis Infection in Mice
Cha, Chun-Nam ; Yu, Eun-Ah ; Park, Eun-Kee ; Choi, Hyunju ; Kim, Suk ; Lee, Hu Jang ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 95~98
DOI : 10.13103/JFHS.2013.28.2.095
Streptococcus suis (S. suis) is a major swine pathogen and an emerging zoonotic agent and is an increasing public health problem across Asia. The present study was undertaken to estimate the antibacterial effect of GR extract and therapeutic effect of GR extract against S. suis infection in mice. At the concentration of GR extract 2.5 mg/ml, the antibacterial effect was not shown on S. suis. However, the antibacterial effect against S. suis was observed at the concentration of GR extract 5.0 mg/ml. Oral administration of GR extract at the dose of 10 mg/kg showed a therapeutic effect for S. suis infected BALB/c mice. The mortality of GR extract-treated mice at the concentration of 5, 10 and 20 mg/kg was 80%, 70%, and 50% at 12 days, respectively, while that of untreated mice was 100% at 8 days after a lethal dose of S. suis infection. The results of our study strongly indicate that GR extract has potential as an effective for S. suis infection in mice.
Forecast of the Field of Research on Food Contaminants in Korea
Sung, Dong-Eun ; Park, Sung Kug ; Kim, Meehye ; Oh, Sangsuk ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 99~107
DOI : 10.13103/JFHS.2013.28.2.099
Food contaminants are an emerging issue because food is highly influential to human health. Delphi survey was performed to establish the plan for effective management of food contaminants. Fifty experts from academia, research institute, government and food industries on food safety and policy participated in this study. From open-end questions 161 topics were collected and classified into five areas and 42 topics as the first round. The five areas were 'the integrated management system on food contaminants', 'safety management of food contaminants derived from environment', 'safety management of natural toxins contaminated on foods', 'safety management of hazardous substances produced during food processing' and 'construction of cooperative system and public relationship'. At the second round, after analyzing the replies (30/50 replied) the preliminary order was obtained and it was confirmed at the third round (24/30 replied). We suggest the order of priority for required research area of food contaminants considering the importance and urgency of the research.
Survey on Comparative Awareness between Media and Industry on Occurrence of Food Safety Incident
Shin, Won-Jung ; Lee, Yu-Si ; Oh, Se-Ra ; Park, Tae-Gyun ; Kim, Renee ; Kim, Ho-Sik ; Lee, Jung-Ho ; Lee, Seung-Yong ; Ha, Sang-Do ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 108~114
DOI : 10.13103/JFHS.2013.28.2.108
To describe the comparative awareness between media and industry, we conducted a survey with a professional group including 28 food safety managers at food corporations and 17 food journalists to find out the differences in perception of 'reporting terms' and 'correspondence terms' when food safety incident occurs. We surveyed by questionnaire methods and did the statistical analysis by SPSS. The results showed that 70.6% of the journalists and 92.9% of the corporation workers answered 'yes indeed' about the question whether the media has a great influence when food safety incident occurs. In the perception that use of the stimulating terms of the media was the cause of company damage, 83% of the corporate workers answered 'yes indeed', and 70.6% of the journalists answered 'yes'. It means that both groups agreed on stimulating terms causing company damage. In the question whether the 'reporting terms' of the media and the 'correspondence terms' of the government and academia made a difference in the consumers' perception and the spread of the incident, 92.9% of the corporate workers and 35.3% of the journalists answered 'yes indeed'. It means that both groups showed a common opinion that the difference in choice of words expanded the perception of consumers, and the spread of the incident. However, the corporate workers agreed with this in a more serious manner than the journalist group. In standardization of 'reporting terms' and restricting use, corporate workers were overall at a positive stand while journalists showed a big difference in opinion within the group. In conclusion, it is necessary to establish a measure to standardize the terms used by corporations, journalists, and professionals in order to decrease the differences of terms and the damage of consumers and the company when food safety incident occurs.
Analysis on the National R&D Portfolio of Food Safety in Korea from 2008 to 2010
Kwak, No-Seong ; Jeong, Jiwon ; Lee, Jong-Kyung ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 115~123
DOI : 10.13103/JFHS.2013.28.2.115
Food safety management should be based on scientific evidences. FAO and WHO presented risk analysis as one of four principles in food safety management. WTO also admits the self safety regulation only when it is made on the basis of risk assessment. Without scientific analysis, tracing and eliminating the cause of food poisoning is impossible. Research and development plays a key role to produce scientific evidences. The Korean government ran over 40 programs in 11 agencies from 2008 to 2010. However, there is no statistics on food safety R&D at present. In this research, food safety projects conducted from 2008 to 2010 are listed up by means of analysing National Science and Technology Information Service (NTIS). The analytical criteria are the name of programs, national standard classification of science and technology, and keywords. As result, Korea Food and Drug Administration, Ministry for Food, Agriculture, Forestry and Fisheries, and Rural Development Administration play major role in the food safety R&D. The portion of more than one year projects should rise up in order to achieve the data for risk assessment, which is strongly required to improve. Besides, the research should be deeper so as to publish more SCI papers. The R&D portfolio should be changed in direction to raise up the portion of biological hazards such as norovirus. In order to do so, a large number of food safety programs should be emerged. The categories of food safety management and the hygiene/quality management of the agricultural and livestock products in the national standard classification of science and technology should be emerged because they are set up reflecting agencies' interests in spite of few differences between them.
Development of PCR Method for Rapid Detection of Allergic Materials in Foods
Park, Yong-Chjun ; Kim, Mi-Ra ; Shin, Jun-Ho ; Kim, Kyu-Heon ; Lee, Jae-Hwang ; Cho, Tae-Yong ; Lee, Hwa-Jung ; Lee, Sang-Jae ; Han, Sang-Bae ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 124~129
DOI : 10.13103/JFHS.2013.28.2.124
The method for detection foods containing allergenic materials by PCR was developed in this study. To detect allergenic raw material from processed food, species specific primer which up to 200bp for PCR product were designed or selected from advanced research. As target materials, 14 items were selected (12 target materials for allergen in Korea, 2 target materials for allergen in foreign countries). The amplicon size for eggs, milk, buckwheat, peanuts, beans, wheat, mackerel, crab, shrimp, pork, peach, tomato, almond, and sesame were confirmed 281, 131, 138, 120, 118, 127, 211, 174, 231, 138, 174, 132, 103, and 220bp, respectively. And any non-specific bands were not detected among each others. Detection method for allergenic material developed in this study could be used to investigate inaccurate goods for allergen labeling or non-intentional contaminant during processed foods manufacturing. In addition, the system will be usefully to detection accurate allergenic raw materials of export for other countries.
Detection Characteristics of Gamma-Irradiated Seeds by using PSL, TL, ESR and GC/MS
Kim, Kyu-Heon ; Son, Jin-Hyok ; Kang, Yoon-Jung ; Park, Hye-Young ; Kwak, Ji-Young ; Lee, Jae-Hwang ; Park, Yong-Chjun ; Jo, Tae-Yong ; Kim, Jae-I ; Lee, Hwa-Jung ; Lee, Sang-Jae ; Han, Sang-Bae ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 130~137
DOI : 10.13103/JFHS.2013.28.2.130
In this study, we investigated the applicability of the photostimulated luminescence (PSL), thermoluminescence (TL), electron spin resonance (ESR) and gas chromatography/mass spectrometry (GC/MS) methods for 5 seeds which are not allowed to be irradiated in Korea. All 5 seeds including evening primrose seed, safflower seed, rape seed, sunflower seed and flax seed were analyzed. Samples were irradiated at 1~10 kGy using a
gamma-ray irradiator. In PSL study, the photon counts of all the unirradiated samples showed negative (lower than 700). The photon counts of irradiated (1, 5, 10 kGy) samples showed positive (higher than 5,000). In TL analysis, results showed that it is possible to apply TL method to all foods containing minerals. In ESR measurements, the ESR signal (single-line) intensity of irradiated foods was higher than non-irradiated foods. The hydrocarbons 1,7-hexadecadiene (
) and 8-heptadecene (
) from oleic acid were detected only in the irradiated samples before and after the treatment at doses
1 kGy, but they were not detected in non-irradiated samples before and after treatment. These two hydrocarbons could be used as markers to identify irradiated safflower seed, rape seed, Sunflower seed and flax seed. And then, the hydrocarbons 1,7,10-hexadecatriene (
) and 6,9-heptadecadiene (
) from linoleic acid were detected in the evening primrose seed, safflower seed and sunflower seed. According to the results, PSL, TL and GC/MS methods were successfully applied to detect the irradiated foods. It is concluded that PSL, TL and GC/MS methods are suitable for detection of irradiated samples and a combined method is recommendable for enhancing the reliability of detection results.
Prevalence and Toxin Characteristics of Microorganism on Hand Towels Using for Children in Child Care Center
Kim, Jung-Beom ; Kim, Nan-Yong ; Kang, Suk-Ho ; Do, Young-Sook ; Eom, Mi-Na ; Yoon, Mi-Hye ; Lee, Jong-Bok ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 138~145
DOI : 10.13103/JFHS.2013.28.2.138
This study was conducted to evaluate the microbiological contamination on commonly used hand towels in the child care centers and to investigate the toxin gene and toxin production ability of food-borne pathogens. A total of 22 commonly used hand towels including 7 for before use and 15 for during use were tested. The average number of total aerobic bacteria and fungi were 6.2 log CFU/100
and 4.1 log CFU/100
. Coliform bacteria were detected in 4 out of 7 before used towels (57.1%) and all of during used towels (100%). These results showed that the sanitary conditions of hand towels in the child care centers should be improved promptly. Among the pathogenic bacteria, Staph. aureus and B. cereus without Salmonella spp. were detected in 5 (22.7%) and 11 (50.0%) out of 22 hand towels. All of Staphy. aureus isolated in this study did not possess any toxin genes and did not produce enterotoxin. The detection rate of hblC, hblD, and hblA toxin genes in B. cereus was 72.7, 72.7, and 54.5% respectively. The possession rate of nheA, nheB, and nheC toxin genes showed 81.8, 72.7, and 54.5% respectively. The cytK and entFM toxin genes were presented at 45.5 and 90.0% in B. cereus. The HBL was detected in 8 out of 11 B. cereus isolates (72.7%) and 5 B. cereus isolates produced NHE (45.5%). Ten out of eleven B. cereus isolates (90.9%) produced one or more enterotoxin such as HBL and NHE. From the results, using a private hand towel or paper towel is required to prevent the cross-contamination between commonly used hand towel and children's hands in the child care center.
Protective Effect of Functional Perilla frutescens Hot-water Extract Against tert-butyl hydroperoxide-Induced Liver Oxidative Damage in Rats
Yang, Sung-Yong ; Kang, Jeong-Han ; Lee, Kwang-Won ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 146~151
DOI : 10.13103/JFHS.2013.28.2.146
Perilla frutescens usually dieted in East Asian country such as Korea and Japan. Antioxidant, antiinflammatory and anticancer activities of perilla leaves have been founded. In previous study, we confirmed that caffeic acid, major compound of perilla, was accumulation by sucrose aqueous solution and thus antioxidant effect of perilla was enhanced. In this study, we investigated the protective effect of functional perilla leaves extract (PLE) against tert-butyl hydroperoxide(t-BHP) induced-oxidative hepatotoxicity. The pretreatment with PLE (250, 500 and 1000 mg/kg b.w.) for 5 days before a single dose of t-BHP (i.p.; 0.5 mmol/kg) significantly lowered the serum levels of aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase dose-dependently. And we confirmed that the indicators of oxidative stress were remarkably reduced in the liver, such as the glutathione contents and malondialdehyde, marker of lipid peroxidation. Pathological histology of the rat livers tissues showed that PLE reduced the hepatocyte degeneration and neutrophilic infiltration of liver induced by t-BHP. These results suggest that functional perilla frutescens has the protective effect of liver against t-BHP-induced oxidative hepatic stress in rats.
Anti-diabetic Effects of Isaria tenuipes in OLETF Rats as an Animal Model of Diabetes Mellitus Type II
Seo, Dong-Seok ; Kang, Jong-Koo ; Jeong, Mi-Hye ; Kwon, Min ; Park, Cheol-Beom ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 152~157
DOI : 10.13103/JFHS.2013.28.2.152
We evaluated the anti-diabetic effects of Isaria tenuipes in diabetes mellitus type 2. For the experiments, the diabetic animal model OLETF rats were divided to 4 groups: Isaria tenuipes was administered mixed with the high fat diet 45% at dose levels of 0.0%, 0.1%, 1.0%, and 5.0% for 4 weeks. All animals have free access to water and high fat diet 45%. The diabetic clinical markers, including clinical signs, body weight and food intake, organ weights, blood glucose level, insulin level and HOMA-IR index, oral glucose tolerance test, GLUT4 mRNA and protein were measured at a time. After administration for 4 weeks, the blood glucose levels, insulin levels and HOMA-IR index of test groups were decreased compared with control group in dose-dependent manner. The body weight and diet consumptions were reduced in control group at 4 weeks. The treatments of Isaria tenuipes also showed high expression of GLUT4 mRNA and protein in the muscle of OLETF rats. The results suggest that Isaria tenuipes has anti-hyperglycemic effect attenuating blood glucose in the animal model of type 2 diabetes and might be useful as a functional diet for human diabetic diseases.
The Safety Assessment of Aflatoxins and Deoxynivalenol in Cereals and Their Products
Kim, Young-Su ; Kim, Young-Sug ; Kim, Myeong-Gil ; Lee, Seong-Bong ; Lee, Ju-Ye ; Oh, Sang-Hun ; Jung, You-Jung ; Seo, My-Young ; Sung, Jin-Hee ; Lee, Woan ; Lee, Jong-Bok ; Yoon, Mi-Hye ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 158~167
DOI : 10.13103/JFHS.2013.28.2.158
This study was carried out to assess the safety of cereals and their products (20 species, 205 samples) distributed in Gyeonggi province by analyzing contamination levels such as exposure frequency of Aflatoxins and Deoxynivalenol (DON). Aflatoxins were detected in 16 (8%) samples in total with a range of 0.01~27.88
, including 3 of 9 bake flour (33%), 2 of 6 dried corn (33%), 3 of 12 frying flour (25%) and 2 of 11 sorghum (18%). DON was found in 56 (27%) samples including 11 of 13 adlay (85%), 6 of 8 wheat (75%), 6 of 10 processed corn for popcorn (60%), 6 of 11 sorghum (55%) and 7 of 16 barley (44%) with a range of 2.2~754.4
. In particular, both Aflatoxins and DON were detected in 8 samples (2 millet, 2 wheat, 1 sorghum, 1 adlay, 1 dried corn, 1 bake flour) simultaneously. As a result of this study, we found that an adult is exposed to Aflatoxins of
b.w./day and DON of 0.18
b.w./day. The quantity of exposure to DON amounted to 18.5 percentage level compared with PMTDI 1
b.w./day suggested on JECFA, therefore we can assess that the possibility of health risks by intake cereals distributed in Gyeonggi province is low. However the concentration of Aflatoxins in one dried corn was 27.88
which was over the maximum residue limits (MRL) suggested on internal and external level, the monitoring about mycotoxin should be conducted on continuously.
Development and Validation of Predictive Models of Esherichia coli O157:H7 Growth in Paprika
Yun, Hyejeong ; Kim, Juhui ; Park, Kyeonghun ; Ryu, Kyoung-Yul ; Kim, Byung Seok ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 168~173
DOI : 10.13103/JFHS.2013.28.2.168
This study was carried out to develop and validate predictive models of E. coli O157:H7 growth. Growth data of E. coli O157:H7 in Paprika were collected at 12, 24, 30 and
. The population increased into 3.0 to 3.8 log10 CFU/g within 4 days, then continued to increase at a slower rate through 10 days of storage at
. The lag time (LT) and maximum specific growth rate (SGR) obtained from each primary model was then modeled as a function of temperature using Davey and square root equations, respectively. For interpolation of performance evaluation, growth data for a mixture of E. coli O157:H7 were collected at time intervals in paprika incubated at the different temperatures, which was not used in model development. Results of model performance for interpolation data demonstrated that induced secondary models showed acceptable goodness of fit. Relative errors in the LT and SGR model for interpolation data (18 and
) was 100%, which show acceptable goodness of fit and validated for interpolation. The primary and secondary models developed in this study can be used to establish tertiary models to quantify the effects of temperature on the growth of E. coli O157:H7 in paprika.
Analyses of Microbiological Contamination in Cultivation and Distrubution Stage of Tomato and Evaluation of Microbial Growth in Tomato Extract
Yun, Hyejeong ; Park, Kyeonghun ; Ryu, Kyoung-Yul ; Kim, Byung Seok ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 174~180
DOI : 10.13103/JFHS.2013.28.2.174
This study investigated the microbiological contamination of tomato in cultivation and distribution stage. Growth of Escherichia coli O157:H7 and Listeria monocytogens examined in tomato extracts (0.1, 1.0, and 10.0%) and incubation temperatures (5, 15, 25, and
). In cultivation stage of tomato, total aerobic bacteria were 7.77 log CFU/g in gloves of APC (Agricultural Products Processing Center) worker and Bacillus cereus were 0.33 log CFU/g at nutrient tank, respectively. And Staphylococcus aureus, Salmonella spp., were not detected. After APC stage, total aerobic bacteria were significantly higher compared with before-APC stage. Among of general, pesticide-free and organic produce in tomato were no significant difference in microbial contamination. Coliforms of tomato in small vinyl package were significantly higher when compared to tomato in whole boxes package. There was no significant difference in bacteria count between unwashed tomato and washed tomato using tap water for one minute. The growth of E. coli O157:H7 and L. monocytogens in tomato extracts were decreased significantly as the concentration increased, and the microbial population was reached the lowest point during storage in 10% tomato extracts concentration for 72h at
. However, the population of E. coli O157:H7 and L. monocytogens were gradually increased at 7.33~8.51 and 7.73~8.60 log CFU/ml during storage at
for 72h, respectively.
Development and Application of DNA Analysis Method for Identificaion of Main Ingredients in Starch
Park, Yong-Chjun ; Kim, Mi-Ra ; Kim, Yong-Sang ; Lee, Ho-Yeon ; Kim, Kyu-Heon ; Lee, Jae-Hwang ; Kim, Jae-I ; Lee, Sang-Jae ; Lee, Hwa-Jung ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 181~187
DOI : 10.13103/JFHS.2013.28.2.181
Identification of main ingredients in starches has been investigated using physicochemical analysis method mainly. However, physicochemical properties such as particle size have limitations in determining the differences among mixed starches. Therefore, we developed a molecular biological method to identify materials used in starch, as a sample, 11 kinds of starches including sweet potato starch, potato starch, corn starch, and tapioca starch. DNeasy plant mini kit, magnetic DNA purification system, and CTAB methods were used to extract DNA from samples. After gene extraction, whole genome amplification (WGA) was performed to amplify the extracted DNA. Species-specific primers were used as followings: ib-286-F/ib-286-R (105 bp), Pss 01n-5'/Pss 01n-3' (216 bp), SS11b 3-5'/SS11b 3-3' (114 bp), and SSRY26-F/SSRY26-R (121 bp) gene for sweet potato, potato, corn, and tapioca, respectively. In this study, we could confirm the main ingredients using WGA and PCR method.
Development and Application of Detection Method for Aphanizomenon flos-aquae not Usable as a Food Materials in Korea
Park, Yong-Chjun ; Shin, Seung-Jung ; Lee, Ho-Yeon ; Kim, Yong-Sang ; Kim, Mi-Ra ; Lee, Sang-Jae ; Lee, Hwa-Jung ;
Journal of Food Hygiene and Safety, volume 28, issue 2, 2013, Pages 188~193
DOI : 10.13103/JFHS.2013.28.2.188
Anatoxin-a, saxitoxin and neosaxitoxin are produced by Aphanizomenon flos-aquae that is a sort of the cyanobacteria phylum. Therefore, it is not permitted for food materials in Korea. Traditionally, the classification of cyanobacteria has been based on morphological characters such as trichome width, cell size, division planes, shape, and the presence of character such as gas vacuole. But, some diagnostic features, such as gas vacuole or akinetes, can show variation with different environmental or growth conditions and even be lost during cultivation. Therefore, we developed detection method for functional foods containing Aph. flos-aquae by PCR. To design the primer, 16S rRNA region of Aph. flos-aquae, Spirulina laxissima, and Spirulina spp. registered in the GeneBank (www.ncbi.nlm.nih.gov) have been used and for comparative analysis, BioEdit ver. 126.96.36.199. was used. As a result, we was design AFA-F1/AFA-R1 (363 bp) primer for the differentiation Aph. flos-aquae from chlorella, spirulina, green tea, and spinach. Also, it could be distinguished chlorella and spirulina products those are made to contain 1% Aph. flos-aquae.