Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of Food Hygiene and Safety
Journal Basic Information
Journal DOI :
The Korean Society of Food Hygiene and Safety
Editor in Chief :
Volume & Issues
Volume 29, Issue 4 - Dec 2014
Volume 29, Issue 3 - Sep 2014
Volume 29, Issue 2 - Jun 2014
Volume 29, Issue 1 - Mar 2014
Selecting the target year
Assessment of the Microbiological Quality of Vegetable from Urban Community Gardens in Korea
Kim, Jin-Won ; Choi, In-Wook ; Na, Won-Seok ; Baljii, Enkhjargar ; Yu, Yong-Man ; Youn, Young-Nam ; Lee, Young-Ha ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 1~5
DOI : 10.13103/JFHS.2014.29.1.001
Many community gardens in large cities worldwide grow vegetables; however, no information regarding the levels of sanitary indicator bacteria and prevalence of foodborne pathogens in vegetables grown in urban community gardens is available. To evaluate the microbiological quality of vegetables from urban community gardens in Korea, 530 samples (nine types of vegetable, including Chinese cabbage, lettuce, radish leaves, spinach, mustard leaves, crown daisy, leek, Korean cabbage, and chicory) were collected at 11 urban community gardens in Seoul, Korea from September through October 2012. The levels of total aerobic bacteria, Escherichia coli, total coliforms, Salmonella spp. Listeria monocytogenes, and E. coli O157:H7 were evaluated quantitatively and/or qualitatively. The mean numbers of total aerobic bacteria and coliforms were 6.3 log CFU/g (range 3.8-8.1 log CFU/g) and 4.3 log CFU/g (range 2.1-6.4 log CFU/g), respectively. Total coliforms were detected on 67% of whole vegetables. Chicory showed the highest number of total aerobic bacteria and coliforms, whereas the lowest number of coliforms was detected on leeks. E. coli was detected on 2.3% of whole vegetables, including lettuce, radish leaves, mustard leaves, and chicory; however, foodborne pathogenic bacteria were not detected on any of the vegetable samples using this highly sensitive and validated procedure. Based on these findings, the presence of coliforms and E. coli demonstrates that opportunity for improvement of microbiological safety exists throughout the produce production chain, although no major foodborne pathogens were present in vegetables grown in urban community gardens.
A Comparison Study of Foreign Nation's Risk Management Programs for Controlling Foodborne Pathogens
Lee, Jong-Kyung ; Shin, Seong-Gyun ; Kwak, No-Seong ; Cho, Yoon-Hee ; Kwak, Hyo-Sun ; Park, Il-Kyu ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 6~15
DOI : 10.13103/JFHS.2014.29.1.006
This study was performed to acquire the information on the foodborne pathogen risk management programs in a couple of developed countries by the expert meeting and searching the information on the web. The backgrounds, strategies and effects related to microbial hazards of the foodborne pathogen reduction programs in fresh produce (US), Escherichia coli O157:H7 in ground beef (US), Salmonella in chicken, pork and eggs (Denmark), and Vibrio parahaemolyticus in seafood (Japan) were investigated for case study. A comparison among the pathogen reduction programs was conducted to find advantages and disadvantages and implications of the policies to bring out implications of the programs. A model for foodborne pathogen reduction program was developed based on both the CODEX risk management scheme and the case studies as follows; 1) preliminary risk management activities, 2) planing a foodborne pathogen reduction program, 3) option identification and selection, 4) implementation (conducting the each stake-holders role and applying the intervention methods), 5) monitoring activities, 6) interim review, 7) continuation or amendment of implementation method by the interim review before achieving the goal, and 8) final review and additional cost-benefit analysis if necessary. This proposed model according to the role of the stake-holders can be used to conduct microbial risk management programs in Korea in the near future.
Determination of Statistical Sampling Plans for Bacillus cereus in Salad and Kimbab
Lim, Goo-Sang ; Koo, Minseon ; Kim, Hyun-Jung ; Kho, Young-Ho ; Park, Kun-Sang ; Oh, Se-Wook ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 16~20
DOI : 10.13103/JFHS.2014.29.1.016
The prevalence of Bacillus cereus was determined in salad and Kimbab obtained from commercial retailers. Among the 100 salad samples analyzed, 54 samples were negative for B. cereus, whereas the bacterial count was < 10 colony forming units (CFU)/g in 8 samples, < 100 CFU/g in 25 samples, < 1,000 CFU/g in 11 samples, and > 1,000 CFU/g in 2 samples. The mean (standard deviation) was 1.18 log CFU/g (
log CFU/g). In Kimbab, B. cereus was isolated from 20 samples; the mean bacterial count was 1.01 log CFU/g (
log CFU/g). On the basis of the monitoring data, a statistical sampling plan was determined with the NEW sampleplan program (ICMSF), which was used as an analytical tool. To identify the most suitable sampling plan, the microbial limits (m, M) and the maximum allowable number of sample units yielding unsatisfactory test results (c) were varied, but the number of samples units, n = 5, was fixed. Sampling plans showing an acceptable probability (Pa) over 0.95 were considered suitable. Two plans (A and B) were finally suggested. Parameters for plan A are n = 5, c = 0, m = 1,000, and M = 10,000 and for plan B are n = 5, c = 2, m = 100, and M = 1,000. Interestingly, the latter plan was identical to the microbial sampling plan used in New Zealand. Thus, it was concluded that the suggested plan can be used as a sampling plan that is in line with international standards.
A Study on Migration of Formaldehyde and Phenol from Melamine-wares
Oh, Jae-Myoung ; An, Mi-Kyung ; Kim, Jin-Chul ; Shin, Dong-Woo ; Park, Chang-Won ; Kim, Meehye ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 21~25
DOI : 10.13103/JFHS.2014.29.1.021
Formaldehyde and phenol used in the production of melamine-wares may be intended to come into foodstuffs. So this study investigated the migration of formaldehyde and phenol from 222 articles Articles were cups(14), bowls(75), plates(85), spoons(10), chopsticks(4), food trays(8), rice paddles(4), spatulas(9) and scoops(12). The food stimulants were 4% acetic acid, 20% ethanol, distilled water and n-heptane. Korea regulation (Standards and specifications for food utensils, containers and package) specifies migration limits for formaldehyde and phenol in food stimulants. Formaldehyde and phenol are restricted by 4 mg/L, 5 mg/L respectively. In all cases the migration of formaldehyde and phenol were below the limit set in Korea regulation. The level of formaldehyde and phenol migrated to food simulants were in the range of N.D~2.949 mg/L, N.D~0.078 mg/L respectively. These migration results of formaldehyde and phenol will provide a scientific basis for the safety management of melamine-wares.
Prevalence and Antibiotic Resistance Patterns in Listeria Monocytogenes Isolated from Food
Jeong, Hyo-Won ; Park, Sang-Hun ; Lee, Jib-Ho ; Kim, Soo-Jin ; Ryu, Seung-Hee ; Song, Mi-Ok ; Park, Sun-Hee ; Jo, Jeong-Yun ; Park, Geon-Yong ; Choi, Sung-Min ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 26~30
DOI : 10.13103/JFHS.2014.29.1.026
Listeria monocytogenes continues to be a important food safety concern. The aims of the present study were to investigate the prevalence and antimicrobial susceptibility of L. monocytogenes. A total of 1,042 samples was collected from 2010 to 2011 in Seoul and twelve L. monocytogenes were isolated. Isolation rate was Gimbap (0.8%), Beef (yukhoe) (2.6%), Pork (cooked) (5.9%), Fish(cooked) (6.3%), and Salmon (11.1%), respectively. In this study, most of the isolates were susceptible to antibiotics. The most common resistance was cefotetan on 11 isolates, followed by cefotaxime on 7 isolates, cepefime on 6 isolates and tetracyclin on 3 isolates.
Quantitative Analysis of Feline Calicivirus Inactivation using Real-time RT-PCR
Jeong, Hye Mi ; Kim, Kwang Yup ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 31~39
DOI : 10.13103/JFHS.2014.29.1.031
Norovirus causes acute gastroenteritis in all age groups and its food poisoning outbreaks are rapidly increasing in Korea. Reverse transcription-polymerase chain reaction (RT-PCR) is most widely used for the rapid detection of foodborne viruses due to high sensitivity. However, the false positive results of RT-PCR obtained against already inactivated viruses could be a serious drawbacks in food safety area. In this study, we investigated a method to yield true positive RT-PCR results only with alive viruses. To decompose the RNA genes from dead viruses, the enzymatic treatments composed of proteinse K and Ribonuclease A were applied to the sanitized and inactivated virus particles. Another aim of this study was to quantify the efficiencies of several major sanitizing treatments using real-time RT-PCR. Feline calicivirus (FCV) that belongs to the same Caliciviridae family with norovirus was used as a surrogate model for norovirus. The initial level of virus in control suspension was approximately
PFU/mL. Most of inactivated viruses treated with the enzymatic treatment for 30 min at
were not detected in RT-PCR, Quantification results to verify the inactivation efficiencies of sanitizing treatments using real-time RT-PCR showed no false positive in most cases. We could successfully develope a numerical quantification process for the inactivated viruses after major sanitizing treatments using real-time RT-PCR. The results obtained in this study could provide a novel basis of rapid virus quantification in food safety area.
Characterization of Bacillus licheniformis SCK A08 with Antagonistic Property Against Bacillus cereus and Degrading Capacity of Biogenic Amines
Lee, Eon Sil ; Kim, Yong Sang ; Ryu, Myeong Seon ; Jeong, Do Yeon ; Uhm, Tai Boong ; Cho, Sung Ho ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 40~46
DOI : 10.13103/JFHS.2014.29.1.040
We have screened Bacillus strains suitable for the fermentation of soybean products with respect to the control of Bacillus cereus and the reduction of biogenic amines. Of 26 isolates, a strain named as the SCK A08 carried antimicrobial activity against B. cereus and Staphylococcus aureus, major food poisoning species in soybean products. PCR analysis revealed that the SCK A08 strain did not contain genes for Bacillus cereus toxins including nonhemolytic enterotoxin, hemolytic enterotoxin, cytotoxin K, cereulide and certrax. The SCK A08 strain could degrade histamine, tyramine, putrescine, and cadaverine by 67.41%, 76.59%, 57.32%, and 50.69%, respectively, during fermentation in cooked soybeans containing 0.5% (w/w) of each biogenic amine. The morphological and biochemical properties and phylogenetic relationships based on 16S rRNA gene sequences indicated that the isolate was most closely related to Bacillus licheniformis. Use of the strain SCK A08 would be a potential measure to overcome two hygienic problems that were frequently faced during manufacture of traditionally fermented soybean products.
Detection of CTX-M Type ESBL Producing Salmonella in Retail Meat in Korea
Kim, Yong Hoon ; Joo, In Sun ; Kim, Yoon Jeong ; Oh, Mi Hyun ; Cho, Joon Il ; Han, Min Kyong ; Kim, Soon Han ; Moon, Tae Wha ; Park, Kun Sang ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 47~52
DOI : 10.13103/JFHS.2014.29.1.047
This study was performed to evaluate antimicrobial resistance of food-borne pathogens isolated from retail meat in Korea. A total of 157 samples of beef, pork, and chicken were collected and analyzed for E. coli, Salmonella, Campylobacter. Resistances to tetracycline were declined in accord with reduced usage of tetracycline in live stock production. E. coli stains from chicken meat had higher multi-drug resistance ratio than strains from other meat. One extended spectrum beta lactamase (ESBL) producing E. coli and two ESBL producing Salmonella were identified in this study. ESBL producing Salmonella strains were confirmed to carry CTX-M-1 type genes.
Antioxidant activities and α-Glucosidase Inhibition Effects of Chicories Grown in Hydroponics Added with Cr
Choi, Jun-Hyeok ; Park, Yun-Hee ; Lee, Sung-Gyu ; Lee, So-Hee ; Yu, Mi-Hee ; Lee, Mu-Sang ; Park, Sun-Hwa ; Lee, In-Seon ; Kim, Hyun-Jeong ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 53~59
DOI : 10.13103/JFHS.2014.29.1.053
This study was carried out to investigate the effect on the growth and antioxidant activities of Cichorium intybus L.(CLE), Cichorium intybus L. var. folisum 'treviso' (CLET), Cichorium intybus L. var. folisum 'rosaitaliana' (CLER) in hydroponics added with
or Selenium (Se) for 4 weeks. Total polyphenol, total flavonoids contents and FRAP values of three species of chicory were grown hydroponically with
or Se were increased. These extracts were also showed stronger DPPH and ABTS scavenging activity than chicory extracts. In particular, chicories added with
had higher antioxidant activities than chicories added with Se. CLER and CLE extracts added with
were also showed
-glucosidase inhibition activities. These results indicate that chicories were cultivated in culture fluid added with
or Se could be used as high functional vegetables.
Bactericidal Efficacy of a Fumigation Disinfectant with Ortho-phenylphenol as an Active Ingredient Against Pseudomonas Aeruginosa and Enterococcus Hirae
Cha, Chun-Nam ; Park, Eun-Kee ; Kim, Yongpal ; Yu, Eun-Ah ; Yoo, Chang-Yeol ; Hong, Il-Hwa ; Kim, Suk ; Lee, Hu-Jang ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 60~66
DOI : 10.13103/JFHS.2014.29.1.060
This test was performed to evaluate the bactericidal efficacy of a fumigation disinfectant containing 20% ortho-phenylphenol against Pseudomonas aeruginosa (P. aeruginosa) and Enterococcus hirae (E. hirae). In preliminary tests, P. aeruginosa and E. hirae working culture suspension number (N value) were
, respectively. And all the colony numbers on the carriers exposed to the fumigant (n1, n2, n3) were higher than 0.5N1 (the number of bacterial test suspentions by pour plate method), 0.5N2 (the number of bacterial test suspentions by filter membrane method) and 0.5N1, respectively. In addition, the mean number of P. aeruginosa and E. hirae recovered on the control-carriers (T value) was
, respectively. In the bactericidal effect of the fumigant, the reduction number of
(d value) was 6.46 and 5.19 logCFU/mL, respectively. According to the French standard for the fumigant, the d value for the effective bactericidal fumigant should be over than 5 logCFU/mL. With the results from this study, the fumigation disinfectant containing 20% ortho-phenylphenol has an effective bactericidal activity, then the fumigant can be applied to disinfect food materials and kitchen appliances contaminated with the pathogenic bacteria.
In Vitro Antibacterial Effect of the Combination of Galla rhois ethanol extracts and Sodium chlorate against Intramacrophage Brucella abortus
Cha, Chun-Nam ; Hong, Il-Hwa ; Yu, Eun-Ah ; Park, Eun-Kee ; Yoo, Chang-Yeol ; Kim, Suk ; Lee, Hu Jang ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 67~72
DOI : 10.13103/JFHS.2014.29.1.067
This study investigated the antibacterial effects of GR ethanol extracts (GRE), sodium chlorate (SC) and a combination of GRE and SC (GS) on Brucella abortus (B. abortus). The antibacterial activities of GRE, SC and GS towards B. abortus were evaluated by incubating B. abortus with GRE, SC and GS. Following treatment with GRE, SC and GS, B. abortus survival and intracellular proliferation in macrophages were monitored. In the cellular cytotoxicity assay, GRE, SC and GS are not cytotoxic at concentrations less than
, 15 mM and 0.6GS (1 of GS, GRE
+ SC 30 mM), respectively. The viability of B. abortus was markedly decreased in a dose-dependent manner in all treatment groups. In addition, B. abortus intracellular proliferation within macrophages was significantly reduced in cells treated with GRE (
), SC (15 mM) and 0.5GS (GRE
+ SC 15 mM) after 48 hr-incubation (GRE, p < 0.01; SC and 0.5GS, p < 0.001). Especially, in the treatment of GS, the synergistic effect of GRE and SC treatment on B. abortus in macrophage was observed. In conclusion, GS is useful as an antibacterial candidate against B. abortus, and can be applied in the field of meat and milk hygiene.
Change in Flavor Components of Black-fermented Garlic Wine according to the Type of Chips during the Manufacturing Process
Kim, Gyeong-Hwan ; Kim, Jin-Hee ; Yang, Ji-Young ;
Journal of Food Hygiene and Safety, volume 29, issue 1, 2014, Pages 73~77
DOI : 10.13103/JFHS.2014.29.1.073
Black fermented garlic includes many pharmacological components. Therefore, in this study, black fermented garlic wine was manufactured and its flavor compounds were investigated difference of aging chips from America and France. The fermented wine was stored at
for 6 months. GC/MS was used for the flavor components analysis. Wine using American chip contained 2-methyl-1-propanol, 3-methyl-1-butanol, 2-methyl-1-butanol, acetaldehyde, butanoic acid, octanoic acid, 1,1-diethoxyethane, and allyl methyl sulfide. 1-Propanol, 2-methyl-1-propanol, 3-methyl-1-butanol, acetaldehyde, acetic acid, propanoic acid, butanoic acid, octanoic acid, 2-heptanone, 1,1-diethoxyethane, N-amino32-hydroxypropanamidate, n-butylamine, and chloroacetonitrile were detected as major flavor compounds using France chips. Especially, the wine contained allyl methyl sulfide that was resulted from black fermented garlic. There were more compounds that smell like fruit in the wine using American chips relatively. And allyl methyl sulfide was detected only in the wine using America chips. Whereas acetic acid was detected only in the wine using France chips.