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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of the Korean Society of Food Science and Nutrition
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Journal DOI :
The Korean Society of Food Science and Nutrition
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Volume & Issues
Volume 22, Issue 6 - Dec 1993
Volume 22, Issue 5 - Oct 1993
Volume 22, Issue 4 - Aug 1993
Volume 22, Issue 3 - Jun 1993
Volume 22, Issue 2 - Apr 1993
Volume 22, Issue 1 - Feb 1993
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Effects of Dietary Vitamin E and Selenium on Hematopoiesis and Antioxidative Detoxification Mechanism in Lead Poisoned Rats
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 651~657
The protective effects of dietary vatamin E and selenium on peroxidative damage and hematopoietic inhibition by lead poisoning were investigated in rats. Male Sprague-Dawley rats weighing 150
5g were divided into six groups according to dietary vitamin E and / or selenium levels, i.e. control(vitamin E, 40mg/kg diet), 0E(without vitamin E, Se), 40E(vitamin E, 40mg/kg diet ; without Se), 200E(vitamin E, 200mg/kg diet ; without Se), 200ES(vitamin E, 200mg/kg diet ; Se, 0.5ppm) and 0Es(without vitamin E ; Se, 0.5ppm) groups. All experimental groups were fed ad libitum 2000ppm lead in diet except control for 4 weeks. Hemoglobin contents and hematocrit values of lead groups were lower than control group except 200ES group and were the lowest in 0E group. Aminolevulinic acid dehydratase(ALAD) activities of blood and liver were sequentially reduced in 200ES, 200E, 0ES, 40E and 0E groups, compared to control, were as urinary aminolevulinic acid (ALA) excretions were increased in the groups which represented low ALAD activity. Heapatic superoxide dismutase(SOD) activities was lower in 0E, and higher in 40E, 200E and 200ES groups, compared with control. Glutathione peroxidase(GPX) activities of liver were reduced in 0E and 40E groups, but those of 0ES, 200E and 200ES groups were significantly increased. Especially GPX activities in 200ES and 200ES groups were not different from control group. The reduced glutathione contents in liver were lowest in 0E and 40E groups, compared with control, whereas levels of the oxidized form were opposite phenomena of that. Liver lipid peroxide values of 0E, 0ES, 40E and 200E groups were 6.4, 2.9, 2.1 and 1.3 fold higher than control, respectively, but 200ES groups was not different from control.
Effect of Dietary Vitamin E on Lipid Metabolism in Caffeine-Fed Rats
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 658~663
This study was designed to investigate the effect of dietary vitamin E levels on lipid metabolism in caffeine-fed rats. Male Sprague-Dawley rats were fed on diet containing one of three levels of vitamin E(37.5, 750, or 1,500mg/kg diet0 which was with or without 0.3% caffeine. The rats were sacrificed after 5 and 10 weeks of the feed periods. Results obtained from this study were as follows ; Net weight gain, feed intake and FER in the caffeine added groups were significantly decreased as compared to those of only vitamin E fed group. Liver and kidney weights tended to increase in the caffeine added groups, but spleen and heart weights were not affected. Total lipid contents in serum and liver tended to decrease as dietary vitamin E became increasing and caffeine diet adding. Serum total cholesterol content tended to increase in the caffeine added group. But, liver cholesterol content were decreased. And the higher the dietary vitamin E level became, the lower serum and liver cholesterol contents were. Serum and liver triglyceride contents were significantly lower in the caffeine added groups than in the only vitamin E fed groups. The degree of decrease was more evident as dietary vitamin E level became more increasing. Serum aminotransferase activity was not affected in all experimental groups.
The Effect of Alcohol Administration on Selenium Concentration and Cell Morphology of Heat and Liver of Rats Fed with the Different Levels of Selenium and Vitamin E
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 664~672
The purpose of this study was to investigate the effect of alcohol administration on selenium concentration and cell morphology in tissurs of rats fed with the different levels of selenium (Se) and vitamin E. Seventy two male rats of Sprague-Dawley strain weighing about 58~62g were divided into 12 groups. The dietary Se levels were 0mg(L-), 0.4mg(C-) and 10mg(H-), and the dietary vitamin E levels were 0mg(-L) and 150mg(-C) per kg diet, respectively. Alcohol-adminstrated groups(--A) received the triple distilled potable water solution containing 10% of ethanol from the 3rd week of experimental periods. The obtained experimental results are summarized as follows. Se concentration in blood and urine made difference in accordance with Se level in diet and tended to be low in alcohol administrated groups. Se concentration in liver and kidney was also directly proportional to the dietary Se level, and it tended to be low in each alcohol group, but Se concentration in kidney tended to be increased by alcohol administration. Myocardium in rats showed lysosome increasing, fat droplet, mitochondrial swelling, and in particular, bad intracellular edema, in H-group fed with high Se and in L-group with low Se. It also showed such phenomena in the alcohol administrated group. In HC-group fed with excessive Se and normal vitamin E. there appeared no noticeable change in liver tissue. However, in the alcohol administrated HCA-group, there came out fat droplet. Especially, in the alcohol administrated LLA-group, not fed with sufficient Se and vitamin, E, there were found lysosome increasing and a number of fat droplet.
Cholesterol-Lowering Effects of Soybean Products (Curd or Curd Residue) in Rats
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 673~677
The effects of soybean products(bean curd and curd residue) on the cholesterol profiles of serum and liver were studied in rats. Male rats were fed on purified diets, supplemented with cholesterol, containing casein (CAS), dried bean curd(DBC), or mixture of casein and dried bean curd residue(CBCD, 3:1, w/w) as a protein source for 4weeks. Dietary DBC and CBCD, compared with CAS, reduced markedly the concentration of serum cholesterol. The concentration of HDL-cholesterol was significantly higher in the CAS group than in the CBCD group, but there were no significant differences in the ratio of HDL-cholesterol to total cholesterol. Liver cholesterol was lower in the DBC group than in the CAS and CBCD groups. The results suggest an effective cholesterol-lowering action of soybean products in rats fed on cholesterolemic diets, and further a possibility that than curd dreg decrease the concentration of serum cholesterol, regardless of the presence of animal protein in diets.
Effect of Dietary Tungstate on the Liver Damage in
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 678~684
To evaluate the role of xanthine oxidase in liver damage by CCl4, a group of rats were fed tungstate for a month, which suppressed the activities of xanthine oxidase in serum and liver. Control group of rats were fed standard diet without tungstate. Liver damage was induced both in tungstate fed and control groups by two intraperitoneal injections of CCl4 at the level of 0.1ml/100g body weight at intervals of 24 hours. Increases in the levels of serum alanine aminotransferase by CCl4 were significantly smaller in tungstate fed rats than in control rats. Concomitantly, histopathologic changes were less in tungstate fed rats than in control ones. In rats either treated with CCl4 or not, hepatic type O xanthine oxidase activities were remarkably reduced by tungstate feeding. Hepatic aniline hydroxylase activities were higher in rats fed tungstate than control rats when animals were not treated with CCl4, but the enzyme activities were lower in tungstate fed rats than control when they were treated with CCl4. Neither tungstate feeding nor CCl4 treatment caused any significant changes in hepatic glutathione contents, and activities of hepatic glutathione S-transferase, glutathione peroxidase and superoxide dismutase. It is concluded xanthine oxidase reaction augment CCl4 induced liver damage via oxygen free radical system.
Effects of the Feeding Mixed Oils with Various Level of n-3 and n-6 Polyunsaturated Fatty Acid on the Lipid Components of Liver, Brain, Testis and Kidney in Dietary Hyperlipidemic Rats
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 685~691
This study was designed to examine effects of the various levels of sardine and safflower oil mix on lipid contents of serveral tissues in dietary hyperlipidemic rats. Experimental oils were 16% butter(control group), 8% butter +8% olive oil(group 2) 8% butter+8% sardine oil(group 3) 8% butter+6% sardine oil+2% safflower oil(group 4), 8% butter+4% sardine oil+4% safflower oil(group 5),8% butter+2% sardine oil+6% safflower oil(group 6) and 8% butter+8% safflower oil(group 7). The diet administered to the male rats of Sprague-Dawley were fed for 4 weeks. In livers, total cholesterol and triglyceride, phospholipid concentrations were lowest in the group 5 and free cholesterol concentrations were lower in the groups 4 and 5, particularly. Total cholesterol and triglyceride concentrations in brain were significantly lower in the group 5 and phospholipids were lowest in the group 3, while free cholesterol were group 7. In testes, total cholesterol and triglyceride, phospholipid, free cholesterol concentrations were lower in the all experimental groups than the control group, but those of kidney were lower in the groups 3, 7 than in the control group. Feeding mixed oil having equal quantity of sardine oil and safflower oil were effective on the reduction of the lipid contents in the principal tissues. It might be due to the effects of appropriate ratios of P/S, 0.85 and n-6/n3-p, 2.85 in the test lipids.
Effects of the Feeding Mixed Oils with Various Level of n-3 and n-6 Polyunsaturated Fatty Acid on the Fatty Acid Metabolism of Brain, Heart and Spleen in Dietary Hyperlipidemic Rats
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 692~701
This study was designed to examine effects of the various levels of sardine and safflower oil mix on lipid contents of serveral tissues in dietary hyperlipidemic rats. Experimental oils were 16% butter(control group), 8% butter+8% olive oil(group 2), 8% butter+8% sardine oil(group 3), 8% butter+6% sardine oil+2% safflower oil(group 4), 8% butter+4% sardine oil+4% safflower oil(group 5), 8% butter+2% sardine oil+6% safflower oil(group 6) and 8% butter+8% safflower oil(group 7). The diet administered to the male rats of Sprague-Dawley were fed for 4 weeks. In the fatty aicd composition of brain phospholipid, n-3 EPA and DHA contents were increased, and DHA content was remarkably high in the phosphatidylethanolamine(PE) component. Arachidonic acid content were high in the cardiolipin component. In the fatty acid composition of heart phospholipid, PUFA contents were highest in the group 5 and DHA content was higher in the groups 4 and 5, particularly. Fatty acid composition of spleen lipid showed that n-3 EPA and DHA contents were higher in the group 3 than in the other groups.
Effect of GE-132 on the Hepatic Bromobenzene Metabolizing Enzyme System in Rats
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 702~708
The study was attempted to elucidate the mechanism of GE-132(100mg/kg, p.o. for 6 weeks) on the metabolism of bromobenzene (460mg/kg, i.p. bid, for 2 days), which has potent carcinogenicity, mutagenicity and hepatotoxicity. It showed that activities of cytochrome p-450, aminopyrine demethylase and aniline hydroxylase, which have epoxide generating property, were not changed by GE-132 treatment. On the other hand, epoxide hydrolase was not changed but that glutathione S-transferase was significantly increased by GE-132 treatment. And also
synthetase was not changed following the GE-132 treatment, but the activity of glutathione reductase was significantly increased. The level of hepatic glutathione which was decreased by bromobenzene recovered markedly by GE-132 pretreatment. It is concluded that the mechanism for the observed effect of GE-132 on bromobenzene metabolism is due to the induction of glutathione S-transferase.
Relationship among Dietary Intakes, Blood Levels, and Urinary Excretions of Ca, P, Mg and Serum Lipid Levels in Korean Rural Adult Men and Women
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 709~715
This study was carried out to estimate the relationship among dietary intakes, blood levels, and urinary excretions of Ca, P, Mg and serum lipid levels in 30 healthy adults living area of Korea(12males and 18 females). Analyses for the nutritional status of the subjects were performed by 3-day dietary intake record, duplicated diet collection, 24-hours urine collection, and venous blood sampling before anthropometry. The mean daily intakes of Ca, P and Mg estimated for 3 days were 452.5
567.8mg and 240.4
78.5mg and the serum concentrations of Ca, P and Mg were 8.9
0.5mg / dl, 3.3
0.6mg / dl, and 2.1
0.8mg /dl, respectively. The 24-hour urinary excretions of Ca, P and Mg were 80.4
161.1mg and 64.8
35.3mg, respectively. The serum concentrations of total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol were 158.8
9.9mg/dl and 92.2
28.5mg/dl, respectively. In the relation between Ca, P, Mg and serum lipids it showed positive correlation with serum Ca and HDL-cholesterol at the level of significance of 5%. It can be concluded that the high level of Ca in serum may be useful in the prevention of cardiovascular disease, but the study verifies the need for more systematic studies.
The Dietary, Blood and Urinary Levels of Lead, Iron and Copper in Self Selected Dietary Rural People
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 716~723
This study was carried out to estimate intake level of Pb, Fe, Cu in rural area of Korea(12 males and 18 females). Analyses for the nutritional status of the record, duplicated diet collections, 24-hour urine collections, and venous blood sampling before measuring of blood pressure. The mean age and blood pressure of the subjects were 45.8
11.1years and 117.5
15.6mmHe in males, and 41.9
8.5mmHg in females, respectively. In respect to both males and females, mean BMI were 22.0
3.0, mean Rohrer index were 131.8
20.2 and mean skinfold thickness were 12.4
7.4mm, respectively. The daily mean intakes of Pb, Fe, and Cu estimated for 3 days were 277.2
7.6mg/day, and 3.0
1.4mg/day in males and 192.0
5.8mg/day, and 3.7
1.7mg/day in females, respectively. The mean concentrations of serum Pb, Fe, Cu were 11.0
/dl in males and 9.0
/dl in females, respectively. The mean levels of Hb and Hct were 15.1
3.3% in males and 13.1
2.6% in females, respectively. The 24-hour urinary excretions of Pb, Fe, Cu were 35.5
/day in males, and 25.3
/day in females, respectively. In conclusion, the Pb intake in self selected diet of this subjects was not in the level that antagonized to Fe and Cu metabolism seriously.
Relationships of Obesity in Childhood to Plasma Lipids, Blood Pressure and Blood Glucose
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 724~733
In order to investigate the relationships of obesity in childhood to plasma lipid, blood pressure and blood glucose concentration, we selected 21 subjects for the moderate obese group(MO), 9 for mild obese group(MI), and 19 for the control group(C) among children aged 10~12. While the level of plasma triglyceride and VLDL-cholesterol of the MO group was much higher than that of group C, a significantly lower percentage of HDL-cholesterol was found in the MO compared to the percentage found in group C. Also the level of the total cholesterol and LDL-cholesterol of the MI group as well as the MO was much higher than that of C. The elevated total-cholesterol level of the Mi group was due to increased LDL-cholesterol and that of the MO was due to increases in both VLDL-cholesterol and LDL-cholesterol. As the result of these differences, the atherogenic index of the MO was significantly higher than that of C. The incidence of hypercholesterolemia( 200mg/dl) of the MI and MO was 60.0% and 77.8% respectively, All of the physical parameters and indexes except height were positively correlated with plasma lipid levels, systolic blood pressure, and blood glucose concentration. The analyses of the correlated with plasma lipid levels, systolic blood pressure, and blood glucose concentration. The analyses of the correlation indicated that central fat to peripheral fat ratio and waist to thigh girth ratio seemed to be closely associated to plasma lipid levels and atherogenic index. The MO had significantly higher systolic blood pressure than C and significantly higher blood glucose concentration was found in both MI and MO than in C. These results confirmed that obesity in childhood may be relevant to chronic metabolic diseases such as abnormal lipid metabolism, atherosclerosis, high blood pressure and diabetes mellitus.
A Computerized Nutrition Counseling System for Patients with Diabetes
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 734~742
A computerized nutrition counseling system for patients having diabetic symptoms has been developed using a personal computer compatible with IBM PC 386. This system is composed of three programs. The first program is designed to find out a personal dietary history and to give suggestions about his incorrect dietary habit. The second one is analyzing the energy and nutrients of food consumed. The analyzed data present the evaluated personal dietary status. With these data, patient could replan his food including snacks as well as regular meals. The third one is the diet and menu planning program that provides the patient with a suggested meal pattern using food exchange table. Practicing these programs, diabetic patient could help himself very conveniently in organizing his meal plan and in improving his dietary behavior.
Effects of Some Natural and Synthetic Gums on the Foaming Properties of Sodium Caseinate
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 743~752
To elucidate the effects of natural gums, i.e. carrageenan, locust bean gum and xanthan gum and synthetic gums, i.e. carboxymethyl cellulose(CMC) and methyl cellulose(MC) on the foaming properties of sodium caseinate, surface tension, absolute viscosity, turbidity, foaming ability, foam stability, and heat stability of the foams of the caseinate solutions with added gums were examined. The surface tension of sodium caseinate solution(5%) adding to gums at pH 4.0 and 5.0 were higher than those at pH 7.0 and 8.0. The viscosities of the solutions adding to gums(0.1, 0.2, 0.3 and 0.5%) ap pH 4.0, 5.0, 7.0 and 8.0 were 2.4~170.0, 2.5~190.0, 5.3~180.0 and 6.3~99.0 cps, respectively. Especially xanthan gums predominantly increased the viscosity. Turbidities were 97.3~98.2 at pH 4.0 and 5.0 and 93.0~95.8 at pH 7.0 and 8.0. Foaming abilities of the solution adding to xanthan gum, carrageenan and locust bean gum were increased, while those of CMC and MC were decreased. Additions of xantham gum, CMC and MC increased the foam stability, especially xanthan gum did predominantly. Xanthan gum, MC and locust bean gum were more effective than CMC and carrageenan for heat stability. For improving the foaming properties of sodium caseinate xanthan gum were best of five kinds of gums. Surface tensions and turbidities of the solution were related to foaming ability, and the viscosities were related to foam stability and heat stability.
Amylograph Pasting Properties of Commercial Acorn Flours
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 753~757
Pasting properties of the two commercial products of air-dried sediment of acorn were studied with viscoamylograph. The concentrations used were 7.0~9.5% and heating temperatures were 86.5
. The viscosity increased as the heating temperature increased at a fixed concentration, but sample A at 8.5% concentration and sample B at 9.5% concentration maintained the constant peak viscosity regardless the heating temperatures. Samples heated to 92.5
showed breakdown at all concentrations. The 15-min height and cold viscosity were negatively correlated with the heating temperature at a constant concentration. There was a 1% difference in concentration to give the same indices of amylogram between two samples.
Effect of Sodium Hexametaphosphate on the Extractability of Sesame Meal Protein and Amino Acid Composition and Color of Its Protein Concentrate
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 758~762
The effect of sodium hexametaphosphate(SHMP) on the extraction of defatted sesame meal protein and the color and amino acid composition of protein concentrate have been studied. The highest amount of protein could be extracted with 1.5% SHMP and the extraction was effective at pH 12.0. The extraction rate tended to increase with increasing the flour to solvent ratio and about 60% of protein was obtained when adjusted the ratio to 1 : 40. Color of sesame protein concentrate was slightly improved by SHMP treatment. Lysine and methionine content were decreased in SHMP-treated protein concentrate but valine and leucine content were increased.
Changes of Protein Composition and Muscle Tissues in Top Shell Meat during Frozen Storage
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 763~770
To investigate the quality changes during frozen storage, top shell, Omphalius pfeifferi capenteri, was stored at -18
immediately after shelling and water holding capacity, protein composition and histological features were examined with the lapsed period of the storage. During the storage period, amount of free drip was increased with higher frozen temperature and longer frozen period, but with the longer storage period, the lower water holding capacity was observed. The extractability and composition of muscle protein, sarcoplasmic protein and stroma protein were rather stable regardless of frozen temperature and frozen storage period. However, the extractability of myofibrillar protein was decreased with higher frozen temperature and longer frozen storage period. On the changes of muscle tissue structure, following points were observed. 1) In the muscle tissue structure of fresh sample, fine muscle fiber was closely distributed all over the tissue regardless of cross and longitudinal section. 2) In tissue structure under frozen state, it was observed that ice crystals apparently grew with the higher storage temperature. Empty spaces between muscle bundles which wee formed by aggregations of muscle fiber were observed after 3 months storage at -18
. 3) Tissue structure in thawed state was restored satisfactorily after 1 month storage regardless of storage temperature. After 3 months storage at -3
, muscle tissue was well restored, but at -18
, empty spaces were apparent due to incomplete restoration.
Effect of Extraction Temperature on Some Quality of Sea Tangle Extract
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 771~776
Aqueous extraction of sea tangle at the temperature range of 60~10
was studied for temperature effects on soluble solids and protein yields, amino nitrogen, turbidity and viscosity of extracts. The solids and protein yield were increased as the temperature increased and most of solids and protein were extracted during 1 hour. The supernatant ratio after centrifugation showed significantly low for the extraction at 6
. More temperature effects were found on turbidity and viscosity than yields. A significantly higher total amino nitrogen contents was obtained from higher temperature at initial stage of extraction and then the differences of them became to be narrow as the extraction prolonged further. The low values of 24.1% solids and 13.5% protein yields after 2 hours of extraction at 10
indicated that most of solids in sea tangle are insouble.
The Bleaching Effects of Potato Lipoxygenase Isoenzymes on
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 777~784
The bleaching effect of potato lipoxygenase Isoenzymes on
was studied. Two lipoxygenase Isoenzymes(LOX-1, LOX-2) from potato tuber were purified by CM-cellulose, DEAE-cellulose ion exchange chromatography. LOX-1 and LOX-2 seemed to have bleaching effect on
in the presence of linoleic acid, which the decrease in the formation of conjugated dienes. LOX-2 was founded to have a greater pigment bleaching activity than that of LOX-1.
Kinetic Studies on the Change of Fructose from the Rice Stored at Fluctuating Temperature Conditions
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 785~791
Kinetic studies were performed to investigate free-fructose content change in rice during storage. The rice was stored at various water activities(0.44, 0.52, 0.65 and 0.75) and at fluctuating temperature(
) over period of 22 weeks. At early stage of storage free-furctose content increased by zero-order reaction, whereas at later stage, it decreased by first-order reaction. The reaction rates were positively related to water activity and/or temperature during storage. Activation energies calculated at range of aw 0.44~0.75 were found to be 10.76~19.56kcal/mole and 4.53~8.40kcal/mole, for the period of increase and decrease in the free-fructose contents, respectively. The shelf-life assessed at
was found to be 252(aw 0.44), 212 (aw 0.52), 193(aw 0.65) and 162(aw 0.75) days.
Synthesis of Alcohol-Oxidase in Candida boidinii
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 792~796
The synthesis alcohol-oxidase[EC 184.108.40.206] was investigated in the yeasts, Candida boidinii CBS 8106 and C. boidinii CBS 2428, during growth on different carbon sources. Alcohol-oxidase was undetectable in all strains submitted to the test in the mineral salts medium containing 1.0% glucose, but its production was rapidly increased when the carbon source was changed glucose to 1.0% methanol after 24hrs of incubation. When cells were grown on the various carbon sources (glucose, xylose, lactose, glycerol, galactose, saccharose, sorbose, lactic acid or acetic acid), the alcohol-oxidase activity was undetected. These carbon sources together with methanol yielded far better synthesis of alcohol-oxidase than in the case of carbon sources alone. Alcohol-oxidase was active towards alcohol of shorter alkyl-chain length than C5 and unsaturated alcohols. Its affinity for these alcohols decreased with the increasing length of the alkyl-chain. The apparent Km values for the methanol of Candida boidinii CBS 8106 and C. boidinii CBS 2428 were 1.96 and 1.21, respestively.
Comparison of the Characteristics of Alcohol-Oxidase by the Various Forms
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 797~802
The properties of the alcohol-oxidase from yeasts assimilating of methanol(Hansenula polymorpha CBS 4732, Pichia pastoris CBS 2612 and Candida boidinii CBS 8106) as free(cellules, purified enzymes) and immobilized forms(immobilized cellules, immobilized enzymes) were investigated. Immobilization enhanced the activity and stability of alcohol-oxidase to a certain degree. The optimum temperature of the immobilized alcohol-oxidase was lower than those of the free forms. The pH / activi쇼 profiles of alcohol-oxidase did not change by immobilization, but changed by the microorganisms. When the immobilized cellules were stocked at 4
in 10mM potassium phosphate buffer(pH 7.5 or 8.0), alcohol-oxidase was more stable than those were stocked in potassium phosphate buffer containing 0.65M sucrose. The immobilization modifies the conditions of oxidation on the various substrates. alcohol-oxidase in immobilized forms showed some with higher Km value for methanol than that in free ones.
The properties of Proteolytic Enzymes from the Fruit of Broussonetia Kazinoki Siebold
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 803~806
The properties of proteolytic enzymes from the fruit of Broussonetia Kazinoki Siebold were investigated. The protease activity of the enzymes from the fruit of Broussonetia Kazinoki Siebold was 1.6 unit. The optimum temperature and pH of the enzymes were
and 7.0, respectively. The enzymes were stable at pH values from 6 to 8 for 1 hr. at
of incubation and also retained all activity after incubation for 1 hr. at
. The enzyme preparations showed strong activities toward hemoglobin and collagen.
Properties of the Extracellular Proteins Produced by Bacillus sp. WY-60
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 807~810
Extracellular proteins of Bacillus sp. WY-60 were obtained, and then the properties of the isolated proteins were characterized. The proteins were composed of two kinds of protein in size. The molecular weight of the major protein was around 21,000 according to the gel filtration chromatography and SDS-polyacryamide gel electrophoresis. The amino acid composition showed that glutamic acid was a major amino acid with the concentration of 26.16mg/g. The isoelectric point of the proteins was about pH 7.5
Studies on the Primary Structure of the Alkaline Protease in Neungee [Sarcodon aspratus (Berk.) S. Ito] I. Amino Acid Composition, Chemical Modification and Sequence of the N-terminal Amino Acid
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 811~814
Properties of a protease purified from Sarcodon asparatus(Berk.) S. Ito have been investigated. The enzyme displays as a glycosylated serine protease. The sequence for the 21 amino acids of the N-terminal side in the enzyme was determined by automated sequence analysis. The sequence was V-T-T-K-Q-T-N-A-P-W-G-L-G-N-I-S-T-T-N-K-L-.
Reverse-Phase High Performance Liquid Chromatographic Determination of Amino Acids after Precolumn Derivatization with 1-Dimethyl-aminonaphthalene-5-sulfonyl chloride
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 815~822
This experiment was carried out to determine the separation condition of 1-dimethylaminonaphthalene-5-sulfony(Dansyl) derivatives of amino acids by reverse-phase high performance liquid chromatography with Nova-Pak C18 column. Determined solvent system was solvent A(200mA phosphate buffer pH 6.8 15%, acetonitrile 11%, water 74%) and solvent B(acetonitrile 65%, methanol 28%, water 7%). Linear gradient of solvent B was applied from 12% to 80% for 50min. Complete separation of 20 amino acids including asparagine and glutamine which constitute protein was achieved within 50min. As the detection limit was the range of picomole, the resolution power was excellent. Reproducibility of the retention time was less than mean
0.05min. According to the above optimum chromatographic conditions, the amino acid composition of some food and human blood was examined. The most affluent amino acid was alanine in human blood, aspartic acid and glutamic acid in soy sauce, alanine and threonine in soy milk and proline in milk and yoghurt.
Alleviating Effects of Vitamin C on the Gramoxone Toxicity in the Total Lipid Contents, Lipid Peroxidation and Protein Patterns of Rat Liver
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 823~832
This study was made to determine alleviating effects of vitamin C(Vt C) on the gramoxone toxicity in rats with respect to the growth gain, feed effeciency ratio, total lipid contents, lipid peroxication and protein patterns in rat liver. Growth gain, feed efficiency ratio and liver weight in the gramoxone group were decreased significantly as compared with the control group, while those values obtained in the gramoxone-Vt C group were increased as compared with the gramoxone group. Lipid contents and TBA value of rat liver of the gramoxone group were increased significantly as compared with the control group. However, those values of the gramoxone+Vt C group were decreased as compared with the gramoxone group. No significant differences were observed in the liver protein patterns between the gramoxone and the control group. The contents of liver protein were decreased significantly in the gramoxone group fed for 2 weeks as compared with the control group. The change of the liver protein patterns, such as the decrease of high molecular weight protein and the increase of low molecular weight protein were observed in the gramoxone+Vt C group.
Purification and Characterization of Lipoxygenase Inhibitor Produced by Penicillium sp.
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 833~838
A strain of Penicillium sp. extracellularly produced an inhibitory substance for lipoxygenase. These purification procedures were followed : ethanol treatment, chromatographies on Dowex 50W, Sephadex G-25, silica gel column and HPLC. The inhibitor was stable in pH range from 3.0 to 5.0 at
, and a treatment at 10
for 2 hours didn't diminish its original activity. The purified inhibitor was charred at temperature near 22
and decomposed. Molecular weight of the inhibitor was estimated to be approximately 270 by Sephadex G-25 column chromatography. The inhibitor rapidly formed EI complex with lipoxygenase and inhibited enzyme activity.
Cellular Resistance to Antifolates
Journal of the Korean Society of Food Science and Nutrition, volume 22, issue 6, 1993, Pages 839~845
One of the major problems of cancer chemotheraphy is the development of drug resistance in tumors, resulting in reduced responsiveness to subsequent treatments. The folate antagonists are being used to treat such diverse illnesses as cancer, leukemia, psoriasis, rheumatoid arthritis, etc. Previous studies have established that resistance to antifolates may occur in mammalian tumor cells by one or more of five mechanisms ; (a) an increase in the levels of the target enzyme, generally as a consequence of gene amplification ; (b) an alteration in the target enzyme, leading to an enzyme with a decreased binding affinity for the drug ; (c) a decrease in the uptake of the drug into the cells ; (d) increased extrusion of drugs out of cells ; (e) impaired ability to polyglutamylate the parent drug which is capable of being intracellularly metabolized to longer chain length.