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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of the Korean Society of Food Science and Nutrition
Journal Basic Information
Journal DOI :
The Korean Society of Food Science and Nutrition
Editor in Chief :
Volume & Issues
Volume 24, Issue 6 - Dec 1995
Volume 24, Issue 5 - Oct 1995
Volume 24, Issue 4 - Aug 1995
Volume 24, Issue 3 - Jun 1995
Volume 24, Issue 2 - Apr 1995
Volume 24, Issue 1 - Feb 1995
Selecting the target year
Effects of Salt and Heparin on the Activation of Lipoprotein Lipase(LPL)
Kim, Tae-Woong ; Lee, Jae-Bok ; Son, Heung-Soo ; Kim, Sung-Wan ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 355~361
Lipoprotein lipase(LPL) is an acylglycerol hydrolase and is the extrahepatic enzyme responsible for the hydrolysis of triglyceride-rich plasma lipoproteins. LPL has been isolated from bovine milk by affinity chromatography on heparin-sepharose in 2M NaCl, 5mM barbital buffer, pH 7.4. Para-nitrophenyl butyrate(PNPB) was used as a substrate for the determination of LPL activity. Molecular weight of LPL was 55KD on 10% SDS-PAGE. When the effects of heparin on LPL activation were compared, LPL activity of heparin added group increased approximately 5 times higher than that of heparin non-added groups. These results indicated that heparin involved in the stabilization of LPL structure that led to increase enzyme activity. Furthermore, LPL activity increased about 4 times compared to the absence of heparin at various pH. LPL was stabilized when heparin was added either low or high salt concentrations. With the presence of heparin, NaCl concentration did not affect LPL activity at pH range 6∼9.
Functional Properties of Modified Low Density Lipoprotein and Degradation of Modified LDL by Human Monocyte-Macrophages
Kim, Tae-Woong ; Park, Jae-Hoon ; Park, Young-June ; Son, Heung-Soon ; Yang, Ki-Sook ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 362~370
Human plasma low density lipoprotein(LDL) is the main carrier for cholesterol, and recent studies suggest the normal LDL can be readily oxidized by free radical and not interact with LDL receptor. Lipoprotein pariticles are consisted of lipid andprotein, and fatty acids of lipoproteins are prone to oxidation. LDL particles readily undergo oxidative modification by copper. From the results, oxidized LDL altered its biological properties. A marked increase in the electrophoretic mobility of LDl on agarose gel indicated that negative surface charge of the LDL particles was increased. Also, the results from the HPLC showed that oxidized LDL was degraded into several polypeptides nonenzymatically. Degradation tests which measured the amount of 5-IAF labelled oxidized LDL were carried out by monocyte and hepatocyte cell culture. Hepatocyte cell culture of modified LDL did not show consistent pattern. However, binding rate of modified LDL with HMDM(human monocyte derived macrophage) was enhanced with oxidation, but was retarded by addition of antioxidants(hyaluronic acid, vitamin A, vitamin E). Also comparisons of oxidized-LDL, acetyl-LDL and MDA-LDL showed significant differences in the chemical properteis and binding affinity to HMDM. Thus, modificaition of normal LDL altered its biological properties.
-Carotene Supplementation on Oxidative Damage by Lipid Oxidation in Rat Liver
Song, Yeong-Ok ; Kim, Hyun-Young ; Jun, Yeong-Soo ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 371~377
The effect of
-carotene supplementation on reducing the oxidative damag in the liver of rats were studied. Forth-five male Sprague Dawley aged 4 weeks were randomly assigned to 9 groups of five for the 12 weeks of the study. Nine groups, sardine oil, sardine oil＋Vt E, sardine oil＋
-carotene, soybean oil, soybean oil＋Vt E, soybean oil＋
-carotene, lard, lard＋Vt E, lard＋
-carotene group, were prepared. Sardine oil, soybean oil, or lard was used for dietary fat and 200% of
-tocopherol or 150% of
-carotene was supplemented to each diet. Each diet supplied 65% of total energy as carbohydrate, 15% as protein, and 20% as lipid. The MDA value and protein carbonyl contents of sardine oil group were significantly different(p<0.05) to those of other fat groups indicating that the most severe lipid oxidation occurred in the group fed diet containing highly polyunsaturated fatty acid. When
-carotene was supplemented to the sardine oil diet, MDA value(-35%, -15%, respectively) and protein carbonyl content(-44%, -32%, respectively) decreased significantly(p<0.05). Cu, Zn-superoxide dismutase(SOD) and catalase activities of three different sardine oil groups with or without antioxidants were lower than those of soybean oil or lard group. The reducing effect of
-tocopherol on oxidative damage in sardine oil group supplemented with
-tocopherol was noticeable(p<0.05). However the adverse effect of
-carotene was observed. SOD and catalase activities of
-carotene supplemented groups were that the lowest among the same fat groups, but the differences were not statistically significant. The possible cause of decreased enzyme activity seemed to be related to the vitamin A(Vt A) toxicity in the liver where retinol converted from dietary
-carotene in the intestinal mucosa was stored.
Effects of Phospholipid Extract from Squid Viscera on Lipid Oxidation of Fish Oil
Hong, Jeong-Hwa ; Jeong, Yong-Sil ; Kim, In-Soo ; Byun, Dae-Seok ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 378~383
Phospholipid(PL), phosphatidylcholine(PC) and phosphatidylcholine free PL(PCF) were extracted from squid viscera and the antioxidant effects of each fraction on the oxidation of refined fish oil were evaluated. Polyunsaturated fatty acid contents were the highest in PC(46.7%) followed by PL(44.8%) and PCF(40.9%). The effects of each phospholipid fraction on stabilizing fish oil were compared by incubating at 40
for 10 days. At the initial period(2 days), changes in peroxide value did not show any significant difference ; however, as incubation time was extended, PC fraction showed the strongest antioxidant activity. PL and PCF added to fish oils also resulted in increased stability against oxidation. Antioxidative effect of PC at the 5% level was equivalent to 0.05% BHT, 1% catechin and 1% tocopherol.
Oxidation Stability Model of Fish Oil
Jeong-Hwa Hong ; Jin-Woo Kim ; Dae-Seok Byun ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 384~388
High content of polyunsaturated fatty acid in fish oil makes it very susceptible to oxidation, which prevent fish oil from successful application to food processing or functional foods. To resolve this problem, oxidation stability model of fish oil was developed using the following differential equation :
. This differential equation can be intergrated using analytical techniques to give :
. At 50, 60, 70 and
were 0.00535, 0.01345, 0.02516 and 0.04675, respectively. The proposed model was well agreed with the measured data except for some minor deviations. In addition,
was expressed as a function of temperature :
. Where T is absolute temperature(
Changes in Solution Properties of Pectins by Enzymatic Hydrolysis of Sidechains
Hwang, Jae-Kwan ; Jozef L. Kokini ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 389~395
The neutral sugar sidechains of apple pectins were hydrolyzed by commercial hemicellulase produced from Aspergillus niger, and the corresponding changes in solution viscosity were investigated in dilute(c
c*) pectin solutions. Pectinase activity included in hemicellulases was removed by Epoxy-activated Sepharose 6B affinity chromatography using polygalacturonic acid as a ligand. Enzymatic hydrolysis of sidechains did not affect the specifc viscosity of dilute(0.5%) pectin solutions, while viscosity significantly decreased in concentrated(2.0∼6.0%) region. These results strongly suggest that the sidechains of pectins exists as an entangled state in concentrated solutions. It was also found that in the concentrated region the extent of viscosity reduction was dependent on pectin concentrations.
Analysis of Dietary Fiber Content of Common Korean Foods
Sung, Chung -Ja ; Hwang, Sun-Hee ; Kim, Jung-In ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 396~403
Eightly nine common Korean foods were analyzed by the enzymatic-gravimetric method developed by Prosky et al. and adopted by AOAC to determine total dietary fiber(TDF) content. The average TDF content of the foods analyzed was 4.78
3.55% for cereal and grain products, 2.56
1.65% for potatoes and starches, 1.94
0.77% for sugar and sweets, 10.81
6.57% for pulse and pulse products, 9.70
5.92% for nuts and seeds, 15..38
15.76% for seasonings, and 4.98
4.35% for beverages. The foods containing the highest TDF values in the food groups were whole grain barley(17.88%) in cereals, yellow soybean(21.05%) in pulses, black sesame(21.34%) in nuts and seeds, and red pepper powder(39.37%) in seasonings except San Cho powder(52.43%). TDF content of rice, the main staple food of Korea, was 2.75% for brown rice and 0.96% for well-milled rice. The TDF value of the foods analyzed ranged from 0.12 to 23.4 times that fo crude fiber reported in the Korean food composition tables. When we consider dietary fiber contents of foods and food consumption patterns together, it is recommended to consume brown rice instead of well-milled rice and increase the consumption of minor cereals and pulses to raise dietary fiber intake.
Dietary Fiber in Godulbaegi(Korean Lettuce, Ixeris sonchifolia H.) Kimchi
Hong-Soo Ryu ; Eun-Young Hwang ; Soon-Sil Chun ; Kun-Young Park ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 404~408
The effect of processing conditions on the changes in the contents of dietary fiber fractions and its physical properteis of Godulbaegi(Korean lettuce, Ixeris sonchifolia H.) was determined during preparation and fementation for kimchi. Water holding capacity(WHC) and oil adsorption capacity(OAC) were also checked on the subject of freeze dried powder from different stages of the kimchi processing. Neutral detergent fiber(NDF) content in young samples(leaf and root) decreased with prolonged soaking and fermentation period. Every young samples had a higher level in NDF than in ripe samples. Noticeable decrease in acid detergent fiber(ADF) without a change in ripe roots was showed after fermentation(
, 60 days). The water holding capacity of freeze dried young plants ranged from 5.78ml/g for roots to 6.31ml/g for leaves. Soaking and fermentation resulted in decreasing WHC and about 50% of WHC(raw leaves) was lowered after kimchi fermentation(
, 40 days). OAC of all samples were lower than WHC in same samples significantly and those were also decreased after soaking and fermentations as WHC.
Antioxidative Characteristics of Dihydroxyphenylalanine, Melanin and Enzymatic Browning Reaction Products of Tyrosine in a Model System
Hong-Sik Cheigh ; Soo-Hyoun Um ; Hae-Gyoung Kim ; Chang Y. Lee ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 409~414
Antioxidative characteristics of dihydroxyphenylalalnine(DOPA), melanin and enzymatic oxidation products of tyrosine(EOPTs) were studied in a model system. EOPTs were prepared by the tyrosine-tyrosinase reaction at pH 6.5 and
at various time intervals(0~120min). All EOPTs were brown in varied intensities with increased absorption at 200~210, 280, 310~320nm, and 450~490nm. EOPTs obtaiend at the early stage of the reaction(1~3min especially) showed a higher antioxidative activity than those from the later stage on the inhibition of peroxide, conjugated dienoic acid and malonaldehyde formations in linoleic acid autoxidation. Additionally among the substances of tyrosine, DOPA and melanin, DOPA showed the highest antioxidative activity while that of tyrosine was the lowest during the linoleic acid autooxidation. It was observed that DOPA and melanin had the ability of free radical scavenging, which may party contribute to their antioxidative activity.
Activity Change of Ornithine Decarboxylase(ODC) after Hepatectomy
Chang Sung ; Jou, Kab-Yeo ; Ha, Duk-Mo ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 415~419
Ornithine decarboxylase(ODC) catalyzes the first and key step in the polyamine biosynthetic pathway. Ornithine decarboxylase is known to the enzyme that increase substantially its activity in regenerating liver. We found that activity and mRNA level for ODC increase significantly after partial hepatectomy in the rat. After laparotomy, there was significant decrease in activity ; however, mRNA content was unaltered in contrast to previous reports of no change in ornithine decarboxylase and thymidine kinase after sham hepatectomy. This may be mediated by the decrease in food intake after hepatectomy. Therefore it is necessary to examine the effect of food intake after hepatectomy on the ODC activity and mRNA level in the future.
Volatile Compounds in Oyster Hydrolysate Produced by Commercial Protease
Cha, Yong-Jun ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 420~426
Volatile compounds in raw oyster and oyster hydrolysate produced with protease were compared by vacuum simultaneous steam distillation-solvent extraction/gas chromatography/mass spectrometry. Sixty-two volatile compounds were detected in both samples. Of these, 57 were positively identified, composed mainly of aldehydes(12), ketones(9), alcohols(14), nitrogen-containing compounds(9), acids(6), terpenes(4), and miscellneous compounds(8). Levels of acids decreased after hydrolysis, whereas several other compounds such as aldehydes, ketones, and nitrogen containing compounds increased. Pyrazines, found in high abundance, were only detected in oyster hydrolysate.
Response Surface Methodology in Development of Oyster Hydrolysate
Cha, Yong-Jun ; Kim, Eun-Jeong ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 427~433
The optimal condition for hydrolysis of oyster was evaluated with proteases using response surface methodology(RSM). Among 11 commerical proteases, APLTM 440 was selected as the suitable protease for producing oyster hydrolysate on the basis of cost per unit enzyme activity. The effect of autolysis on degree of hydrolysis in oyster was negligible comparing to that of APL 440 protease treatment. From RSM and ridge analysis, the conditions favoring the highest degree of hydrolysis were pH 9.95, 61.1
, 2.64 hr reaction time, 49.2% substrate, and 0.35% enzyme/substrate ratio. Oyster hydrolysate prepared under optimal conditions shwoed virtually 51.98% of hydrolysis.
Chinese Radish Juice as a Growth Substrate for the Production of Candida utilis ATCC 42416 Biomass
Hyun Ku Kim ; Sue-Young Suh ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 434~439
Chinese radish juice(CRJ) was used as a culture medium for the production of Candida utilis ATCC 42416 biomass. Soluble solid and total sugar contents of Chinese radishes were in the range between 5.5 and
Brix and 3.5 and 6.5%, respectively. Since sugar in radishes are in readily usable forms, pretreatm ent procedures were not necessary after the extraction of juice from fresh radishes. In shake flask experimetns, C. utilis ATCC 42416 grew well in CRJ and completed growth in 24 hrs at
and 200 rpm. Maximum cell dry weight obtainable from a liter of CRJ(1.0% sugar
) was 21.5g, when the yeast was grown on CRJ diluted 5 times or more with tap water to make sugar content to be eual to or less than 1.0%. Supplementation of 5-fold diluted CRJ with some nutrients did not greatly influence the growth rate, yeast biomass production, or cell protein content significantly, indicating that CRJ itself was a good substrate for the production of biomass by C. utilis ATCC 42416.
Antimutagenic Effects of Juices from the Peppers in Salmonella Assay System
Park, Kun-Young ; Kweon, Young-Mi ; Rhee, Sook-Hee ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 440~445
The antimutagenic effects of juices from green pepper(GP), red pepper leaf(RPL), red pepper(RP) and sweet pepper(SP) were examined by the Ames method using Salmonella typhimurium TA100. The juice supernatants of GP, RPL and RP showed antimutagenic activities against afltoxin B1(AFB1) in Salmonella typhimurium TA 100. The juice supermatants of GP and RPL also exhibited the inhibitory effects(p<0.05) to the mutagenicities induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and 4-nitro-quinoline-1-oxide(4-NQO). The juice of RP showed antimutagenic activities against indirect mutagen of AFB1, however, the activity was reduced at higher concentration(5.0%), furthermore, as the adding concentration of sample increased to 5.0%, it exhibited slight comutagenicith on direct mutagen of MNNG. Theantimutagenic activities of GP and RPL juices were reduced significantly after heating at 100℃ for 20min, supposing that the antimutagenic compound(s) in the juices were heat labile.
Changes in Hydrophobic Surface of Collagen by Chondroitin Sulfate : Fluorescence Intensity Measurements with Bis-ANS as the Probe
Kim, Sung-Koo ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 446~453
The improtant components of extracellular matrix(ECM) are collagen and chondroitin sulfate. The hydrophobic surface of collagen is one of the determining factors of diameter of collagen fiber and also is closely related to the aging phenomena. The controlling mechanism of the diameter of collagen fiber influenced by the interaction with chondroitin sulfate was evaluated using bis-ANS as a hydrophobic probe. Hydrophobic surface area of collagen molecule shielded by chondroitin sulfate was evaluated. Relative fluorescence intensity of collagen in thepresence of chondroitin sulfate was measured using bis-ANS as a hydrophobic probe. The fluorescence intensity decreased with the increase in chondroitin sulfate up to 3.8 chondroitin sulfate/collagen(mole/mole). Further increase in the ratio of chondroitin sulfate to collagen did not change the fluorescence intensity. Similar changes in the relative fluorescence intensity were observed for both rat tail and lathyrific rat skin collagen. The fluorescence intensity indicated by the binding between bis-ANS and hydrophobic sites of collagen was pH dependent, and the shielding effect of collagen-chondroitin sulfate interaction could not be detected at pH above 6.0. This is probably due to the charge repulsions caused by negative charged collagen molecules at higher pH.
Quantitative Analysis of Alkylpyrazines in Snow Crab Cooker Effluents
Cha, Yong-Jun ; Baek, Hyung-Hee ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 454~458
Alkylpyrazines in snow crab cooker effluent(SCCE) and effluent concentrate(EC) were quantitatively analyzed and compared by simultaneous steam distillation-solvent extraction/gas chromatography/mass spectrometry(SDE/GC/MS). A total of 11 pyrazines were identified in both SCCE and EC. Amounts of tetramethylpyrazine, 2,5-dimethylpyrazine, trimethylpyrazine, and 2,6-dimethylpyrazine were 23.0, 21.1, 13.8, and 13.3 times higher, respectively, in EC than those in SCCE. The total amount of pyrzines in EC (1664.0
171.1ng/g) was 8.1 times higher than that in SCCE(204.5
32.2). The compounds, ethylpyrazine and 2-ethyl-3,6-dimethylpyrazine, were only detected in EC.
GC-MS Analysis of Diterpene Quinone Constituents of Salviae Miltiorrhizae Radix and Biological Activity
Park, Hee-Juhn ; Lee, Seung-Bae ; Lee, Eun ; Cha, Bae-Chun ; Park, Moo-Young ; Lee, Sung-Mok ; Chung, Won -Tae ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 459~465
The ether extract of Salviae miltiorrhizae Radix(SMR) was fractioned to give five subfractions, so that two subfractions of them were recrystallized to yield each pure diterpene quinone pigment. On the basis of spectral evidence, these two compounds were identified as tanshinone II and crytotanshinone. Cryptotanshinone exhibited both of a potent platelet anti-aggregating activity in vitro and a potent antimicrobial activity. GC-MS analysis of the other extract showed that tanshinone II was contained in the largest proportion of all the diterpene quinones. In addition, GC-MS analysis gave other valuable analytical informations.
Antiviral Effect of Lithium-Ascorbate Derivatives
Kil-Jin Kang ; Akira Murata ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 466~469
The effect of lithium-ascorbate derivatives on viruses was investigated using a wide variety of bacterial viruses(phage). Lithium-ascorbate derivatives exerted an inactivating effect on all phages examined. Lithium-ascorbate derivatives have antiviral effects. The antiviral effect of lithium 2-o-octadecyl ascorbate was stronger than that of lithium ascorbate. Even at 10∼20 times lower concentration, the lithium 2-o-octadecyl ascorbate showed very much similar phage inactivating effect to that of ascorbate and lithium ascorbate.
Study of Rat Mammary Epithelial Stem Cells In Vivo and In Vitro
Nam Deuk Kim ; Kee-Joo Paik ;
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 3, 1995, Pages 470~486
Mammary epithelial cells contain a subpopulation of cells with a large proliferativ potential which are responsible for the maintenance of glandular cellularity and are the progenitor cells of mammary cancer. These clonogens give rise to multicellular clonal alveolar or ductal units(AU or DU) on transplantation and hormonal stimulation. To isolate putative mammary clonogens, enzymatically monodispersed rat mammary epithelial cells from organoid cultures and from intact glands are sorted by flow cytometry according to their affinity for FITC labeled peanut lectin(PNA) and PE labeled anti-Thy-1.1 antibody(Thy-1.1) into four subpopulations : cells negative to both PNA and Thy-1.1(B-), PNA+cells, Thy-1.1+cells, and cells positive to both reagents(B+). The in vivo transplantation assays indicate that the clonogenic fractions of PNA+cells from out-growths of organoids in primary cultures for three days in complete hormone medium(CHM) are significantly higher than those of cells from other subpopulations derived from cultrues or from intact glands. Extracellular matrix(ECM) is a complex of several proteins that regulated cell function ; its role in cell growth and differentiation and tissue-specific gene expression. It can act as a positive as well as a negative regulator of cellular differentiation depending on the cell type and the genes studied. Regulation by ECM is closely interrelated with the action of other regulators of cellular function, such as growth factors and hormones. Matrigel supports the growth and development of several different multicellular colonies from mammary organoids and from monodispersed epithelial cells in culture. Several types of colonies are observed including stellate colonies, duct-like structures, two- and three-dimensional web structures, squamous organoids, and lobulo-duct colonies. Organoids have the greatest proliferative potential and formation of multi-cellular structures. Phase contrast micrographs demonstrate extensive intracellular lipid accumulation within the web structures and some of duct-like colonies. At the immunocytochemical and electron micrograph level, casein proteins are predominantly localized near the apical surface of the cells or in the lumen of duct-like or lobulo-duct colonies. Squamous colonies are comprised of several layers of squamous epithelium surrounding keratin pearls as is typical fo squamous metaplasia(SM). All-trans retinoic acid(RA) inhibits the growth of SM. The frequency of lobulo-ductal colony formation increased with the augmentation of RA concentration in these culture conditions. The current study models could provide powerful tools not only for understanding cell growth and differentiation of epithelial cells, but also for the isolation and characterization of mammary clonogenic stem cells.