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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of the Korean Society of Food Science and Nutrition
Journal Basic Information
Journal DOI :
The Korean Society of Food Science and Nutrition
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Volume & Issues
Volume 24, Issue 6 - Dec 1995
Volume 24, Issue 5 - Oct 1995
Volume 24, Issue 4 - Aug 1995
Volume 24, Issue 3 - Jun 1995
Volume 24, Issue 2 - Apr 1995
Volume 24, Issue 1 - Feb 1995
Selecting the target year
Study on the Antioxidative Activity of Human Milk
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 651~657
The antioxidative activity of each fraction in human milk was examined using H2O2 and FeSO4-induced lipid peroxidation of mouse liver homogenate in order to elucidate the antioxidative substances of human milk. High molecular weight(~>20KD) fraction had more antioxidative effect on lipid peroxidation than low molecular weight(~ <20KD) fraction. Furthermore, the changes of antioxidative enzyme activities were estimated during lactation to study the roles of human milk. The human milk showed high activities of catalase, glutathione(GSH) peroxidase and GSH S-transferase. These results suggest that the antioxidative activities may mostly be attributed to high molecular weight fraction containing catalase, GSH peroxidase and GSH S-transferase.
Effect of Schizandra chinensis Extract in Hyperlipidemic Rats
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 658~662
The purpose of this study was to investigate the effect of Schizandra chinensis extract on the serum and hepatic lipids inhyperlipidemic rats fed with high-fat diet. The Schizandra chinensis extract decreased significantly in total serum cholesterol, serum triacylglycerol and heptic triacylglycerol but not in the total hepatic cholesterol. The Schizandra chinensis extract also decreased in very low density lipoprotein increased by the highfat diet without affecting high density lipoprotein cholesterol. These results suggest that Schizandra chinensis extract may be benificial for the regulation of hyperlipidemia.
Dietary Patterns of Elderly People by Smoking Status
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 663~675
It has been proposed that differences in diet between smokers and nonsmokers may partially explain the positive association between cigarette smoking and chronic diseases such as cancer and coronary heart disease. To investigate the potential for this confounding, the authors studied the relation between cigarette smoking and dietary pattern including nutrient intake in 152 Korean elderly people aged 59∼84years. Dietary intake was determined by a 24-hour recall method and a food frequency questionnaire. Male smokers were lighter than nonsmokers and had a lower body mass index(BMI)(p<0.05). But there were no differences in body weight and BMI between female smokers and nonsmokers. Female smokers had lower intakes of energy and carbohydrates than nonsmokers(p<0.05), but in male, smokers and nonsmokers did not show the difference in energy intake. The mean intake of fiber, protein, fat, minerals, and vitamins did not vary significantly by smoking status. However, both male and female smokers consumed fewer fruits than nonsmokers and ate fresh fruits less often than nonsmokers. The total food frequency score which reflect dietary quality of smokers was lower than those of nonsmokers(p<0.05). Male exsmokers' diet were similar to those of smokers, but in female, dietary patterns of exsmokers were similar to those of nonsmokers. These results confirm that elderly smokers have partially different nutrient and food intake patterns from nonsmokers. The decreased ingestion of fruits and less balanced diet by cigarette smokers could contribute to their increased risks of chronic disease compared with those by nonsmokers. The increased ingestion of nutrients and improved nutritional balance in the diet of exsmokers may help to reduce their risks as well.
A Study on the Nutritional Knowledge, Food Habits, Food Preferences and Nutrient Intakes of Housewives Living in Urban Area and Rural Area
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 676~683
This nutritional survey was conducted from January to March 1994, in order to investigate the nutirtional knowledge, food habits, nutritional attitude, food preferences and nutrient intakes of housewives living in urban area and rural area. The subjects of this survey were 164 urban housewives living in Daegu and Pohang area and 170 rural housewives living in Andong area. The results obtained are summarized as follows ; The subjects had a high level of perceived knowledge(81.2%), that is the knowledge that each subject believed she had, but the accuracy of the knowledge was only 66.3%. Most of the urban housewives belong to "Fair" or "Good" food habit group, while rural housewives belonged to "Fair" or "Poor" food habit group. The correlation between nutritional knowledge score and food habit score was low. And the correlation between nutritional attitude score and food habit score was low, too. Food perferences for kimchi, cooked rice, potato, milk were high in both group. Most of the nutrient intakes of the urban and rural housewives were not significantly different each otehr at p<0.05.ficantly different each otehr at p<0.05.
Structure and Properties of Starch on Rice Variety
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 684~689
The molecular structure of rice starch was investigated using Korean rice[3 varieties of Japonica type and 3 varieties of Tongi type(Japonica-Indica breeding type)]. The λmax of iodine complex and inherent viscosity of Japonica type were higher than those of Tongil type.
-Amylolysis limit of the starches was not different between the two rice types. In the distribution of molecular weight of rice starch, the molecular size of amylose and amylopectin for Japonica type were smaller than those for Tongil type. The chain of rice starch distributed F1 of above DP 55, F2 of DP 40~50 and F3 of DP 15~20, and the ratio of F3 against F2 for Japonica type was higher than that of Tongil type. The results suggest that rice of Japonica and Tongil type was different molecular structure of starch.
A Safety Test on Grapefruit Seed Extract
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 690~694
Effects of grapefruit seed extract on the lethality, the primary skin irritation, and the acute eye irritation potentials were evaluated in rats. The acute oral
of GFSE for Sprague-Dawley derived rats was 3.75g/kg with 95% confidence limits of 2.55 and 5.52g/kg. Patch application to the intact and abraded skin of rabbits led to mild to moderate erythema and no or mild edema. Application to the eyes of rabbits led to severe corneal opacity, iritis, conjunctival erythema, edema, and discharge.
Isolation and Antimicrobial Activity of Antimicrobial Substance Obtained from Leaf Mustard(Brassica juncea)
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 695~701
The ethanol extract of leaf mustard(Brassica juncea) exhibiting high antimicrobial activities was fractionated in the order of hexane, chloroform, ethylacetate and butanol fractions to test antimicrobial activity. The highest antimicrobial activity for the bacteria tested was found in the ethylacetate fraction, but a lesser extent in the butanol fraction. In contrast to antimicrobial activity for the bacteria, both ethylacetate and butanol fractions showed weak antimicrobial activity for yeasts. Unknown compound A in the ethylacetate fraction which exhibited a strong antimicrobial activity was isolated by silica gel column chromatography and HPLC, and exhibited 9 times more antimicrobial activity than the ethylacetate fraction.
Structural Analysis of Major Antimicrobial Substance Obtained from Leaf Mustard(Brassica juncea)
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 702~706
A major component(compound A) in the ethylacetate fraction exhibited a strong antimicrobial activity was identified by UV, IR, FABMS and NMR. The compound A showed strong absorbance at 209, 259 and 359nm, indicating a flavonoid ring structure. IR spectrum possessed absorbance of OH at 3400∼3300cm-1, ketone at around 1650cm-1, and aromatic C=C at around 1660cm-1. Molecular weight of the compound A calculated as 478 from the information of m/z 479(M＋H)+ and m/z 477(M-H)+ in the FABMS spectrum. Molecular formula of this compound was found to be C22H22O12 from m/z 479.1220(＋3.1mmu for C22H23O12) of HRFABMS spectrum and from 13C-NMR spectrum. 1H-NMR and 13C-NMR spectra of the compound A revealed aromatic proton and benzene rings. Distortionless enhancement by polarization transfer(DEPT) exhibited that the compound A possessed 10 quaternary carbons and 3 substituted benzene rings including a methoxy group substitution. The compound A was identified as isorhamnetin 3-O-β-glucopyranoside by spectrophotometric methods in conjunction with 1H-1H COSY, 1H-13C COSY and HMBC, which revealed a flavone with OH group at 3, 5, 7, and 4' and methoxy group at 3' positions esterified to glucose.
Antimicrobial Activity and Heat Stability of Water-Pretreated Extract of Leaf Mustard Dolsan(Brassica juncea)
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 707~712
Natural antimicrobial activities in leaf mustard Dolsan(Brassica juncea) were evaluated for food preservation. Antimicrobial activities and changes in volatile allylisothiocyanate(AIT) concentration were examined during incubation of leaf mustard homogenate for 72 hours at
. The concentration of volatile AIT was highest at 2 hours of incubation. Antimicrobial activities, which were insignificant in raw homogenate without incubation, were occurred at 21 hours and were showed highest at 48~72 hours of incubation. Water-pretreated extract(WPE) showed the antimicrobial activity by 1.2~1.4 times higher than the water extract. Ethylacetate fraction of leaf mustard showed high antimicrobial activities. The WPE had strong antimicrobial activities against Salmonella typhimurium and Staphylococcus aureus. Heat treatment of the WPE for 30 min at
did not lose the antimicrobial activities.
The Effects of Puerariae Radix Catechins Administration on Liver Function in Carbon Tetrachloride-Treated Rats
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 713~719
These studies were carried out to investigate the effects of Puerariae radix catechins(PRC) administration on the biochemical parameters of liver function in liver of carbon tetrachloride(CCl4)-treated rats. Thirty six healthy Sprague-Dawley rats weighing about 120g were used for this experiment and divided intot he following 3 groups : normal control group(NCON),
control group(CCON), PRC treated group(PRC). Fifty percent
in oil was administered(I.P.) by 2ml per kg body weight two times a week for 3 weeks. PRC treated groups were administered orally at the leaves of 1% per day in distilled water for 8 weeks. Lipid hydroperoxides were analyzed by using chemiluminescence-high performance liquid chromatography(CL-HPLC) method as a phosphatidylcholine hydroperoxide value(PCOOH) in liver tissues.
treatment significantly(p<0.05) resulted in an increase in GPT & GOT activities and liver hydroperoxide values comparing with those of the untreated control, while administration of PRC to the
rats significantly(p<0.001) decreased GPT & GOT activities and liver hydroperoxide value. Their ultrastructual changes of hepatocellular organelles were shown to clarify the morphologic nature of protective effects of PRC on hepatocytic injuries.
treatment observed to change the ultrastructual nature of outer membrane of hepatocytes. However, the hepatic changes on PRC treatment to
group was not found. PRC administration may inhibit the formatiion of liver lipid hydroperoxides in vivo and were very effective in recovering the liver function in
The Chemical Composition of Persimmon (Diospyros kaki, Thumb) Leaf Tea
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 720~726
Chemical components relevant to the characteristic taste of the Korean native persimmon(Diospyros kaki, Thumb) leaf tea were analyzed. Samples were processed by using three different methods ; SHT(steamed and then hot-air dried), DHT(dried in the shade, steamed and then hot-air dried) and RHT(roasted and then hot-air dried). The components analyzed were general compositions of dried perisimmon leaves and extracted solution. The composition of moisture, ash, crude lipid and total nitrogen did not show significant variation among different processing methods of the persimmonleaf tea. The contents of caffeine, tannin and vitamin C in persimmon leaf tea were in the range of
, 29.1~38.5mg% and 325.3~2084.7mg%, respectively. The vitamin C content was significantly higher in the RHT than other treatments. The contents of caffeine, tannin and vitamin C in the tea extracted solution were in the range of
, 15.4~25.9mg% and 111.0~1274.3mg%, respectively. The vitamine C in the tea solution was the highest in the RHT treatment and 61.1% of vitamin C in the leaf tea was extracted out in these processing methods. The major amino acids contained in the leaf tea were in decreasing order glutamic acid, aspartic acid, leucine and phenylalanine, these four amino acids consisting 38.9~39.8% of the total amino acid contained in the persimmon leaf tea. The major amino acids contained in the tea solution were glutamic acid, proline, histidine and arginine. Six kinds of 5'-nucleotides, CMP, AMP, UMP, IMP, GMP and hypoxanthine were detected and CMP was the most abundant component in fresh leaf, leaf tea and tea solution. The second highest 5'-nucleotides in both leaf tea and tea solutions were GMP, AMP and UMP in all processing method. The highest free sugar contained in the fresh leaf tea and tea solution was sucrose.
The Components of the Sap from Birches, Bamboos and Darae
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 727~733
This stduy was attempted to establish the basic data for evaluating chemical components in the sap from birches(Betula platyphylla Sukatschev, Betula costata Trautv, Betula davurica Pallas), bamboos(Phyllostachys pubescens, Phyllostachys bambusoides, Phyllostachys nigra), Darae(Actinidia arguta). Calcium and potassium in five kinds of mineral detected in the sap were dominant mineral, magnesium, sodium and iron in order and calcium, potassium and magnesium are abundant in the sap from bamboo more than the other sample and the contents were 242.0~422.1mg/L, 793.8~ 2504.1mg/L and 72.6~165.9mg/L, respectively. Free sugars of the sap determined were glucose, fructose and sucrose, but maltose was not detected. The contents of glucose and fructose of the sap from Betula platyphylla Sukatschev(#2) were the highest and 42.1g/L and 36.9g/L, respectively. The detectabel nucleotides and their related compounds were CMP, UMP, GMP, IMP, AMP and hypoxanthine. The total contents of composition amino acids detected from eighteen kinds of the sap were in the range of 2.4~30.4mg%. The major amino acids were taurine, glycine, lysine, alanine and threonine in the sap from birch(#1, #2), glutamic acid and lysine in the sap from Betula costata Trauty(#3) and Betula davurica Pallas(#4), lysine, valine, alanine, serine, tyrosine and glutamic acid in the sap from bamboos, and glutamic acid, leucine, alanine in the sap from Darae.
Kinds and Characteristics of Traditional Special Kimchi in Pusan and Kyungnam Province
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 734~743
This study was conducted to summarize the known kinds and characteristics of traditional special kimchies in Pusan and Kyungnam province. The taste of kimchi varies from region to region within Korea. In the southern part of the country, where the weather is much warmer, more salt is added along with salted anchovy(Myulchi Jeot), and the kimchi is less juicy and richer in flavor. More than 180 different varieties of kimchi are reported in Korea, but there are 30~40 different kinds of kimchi in Pusan and Kyungnam province depending on the main ingredients are known. Puchu kimchi(leek kimchi), Uung kimchi(burdock kimchi), Kongnip kimchi(soybean leaf kimchi) are the typical traditional special kimchi in Pusna and Kyungnam province. Todays, dietary cultures has been gradually changed, and diminished the local characteristics. Also, these tendency appears in the taste of the traditional special kimchi. However, it still remains the distinciton in the taste, ingredients and the methods of preparation of kimchies of Pusan and Kyungnam province. In this paper, the dispersed informations on the kinds, characteristics, and the preparation method of the special kimchies known in literatures and inherited persons in Pusan and Kyungnam province were tried to put together.
Effect of Licorice Root(Glycyrrhiza Uralensis Fischer) on Dongchimi Fermentation
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 744~751
Effect of licorice root(Glycyrrhiza Uralensis Fischer) on the Dongchimi(watery radish kimchi) fermentation was investigated by measuring physicochemical, microbiological and sensory properties during fermentation up to 41 days. Dongchimi with the various levels(0, 0.5, 1.0, 1.5%) of licorice root was fermented at two temperatures : Group A was fermented at
and group B was fermented at
after keeping at room temperature(
) for 24 hours. During the fermentatiion, pH was slowly lowered in all Dongchimi samples and pH of Dongchimi added licorice root was a little higher than that of Dongchimi without licorice root. Total acidity of group A was slightly larger than that of group B and Dongchimi added licorice root showed small content than that of Dongchimi without licorice root. Reducing sugar content was reached maximum value in 13 days of fermentation in Dongchimi added licorice root of group A and B, group A decreased remarkably in 32 days of fermentation in all samples and group B was maintained in the rest sample except for Dongchimi without licorice root and Dongchimi added 1.5% licorice root. Total vitamin C of Dongchimi added licorice root was slightly higher than that of Dongchimi without licorice root of two groups. Lightness lowered gradually with the fermentation and Dongchimi without licorice root decreased a little than that of Dongchimi added licorice root. The number of lactic acid bacteria in Dongchimi added licorice root was more numerous than that of Dongchimi without licorice root. As a result of the sensory evaluation, group B showed higher scores than group A and Dongchimi added 0.5% licorice root was the most preferable one.
Effect of Fermentation Temperature on the Physicochemical Properties of Mustard Leaf(Brassica juncea) Kimchi during Various Storage Days
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 752~757
The effect of fermentation temperature on the changes of pH, acidity, salt content, color and vitamin C of mustard leaf kimchi during various storage days was investigated. The conditions of fermentation temperature were set at
(sample A) and
(sample B), and
after keeping at
for 12 hours(sample C) and
for 36 hours(sample D). As the fermentation proceeded, pH of sample stored at low temperature(sample A) was drop ped gradually from initial pH of 5.24 but there was great pH drop in the sample stored at high temperature(sample B, D). The salt content of the sample B at high temperature increased remarkably, and then the values showed D > A > C. The Humter values of L and a increased at the optimum ripening period, the higher the initial fermentation temperature(B) and the later the initial fermentation time at
those values, then decreased. The Hunter value of b constantly increased until day of 108. As fermentation time passed, the content of total vitamin C decreased to the range of 9.0mg% to 14.0mg% up to 24 days of fermentation, and at the optimum ripening period, it increased to the range of 14.0mg% to 22.0mg%, and at the fermentation period(until day 108), it decreased gradually.
A Survey on the Children한s Notion in Kimchi(I) - Children한s Preferences for Kimchi -
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 758~764
A survey on the kimchi preference among elementray school students in Pusan was conducted in April of 1995 to get basic information needed for the development of special kimchi for the children. Total of 1100 children in 2nd, 4th and 6th grade from public and private elementary school participated in this survey. Sixty eight percent of students answered that they like kimchi. However, kimchi preference(17.6%) among other side dishes-pork cuttlet.ham.sausage(54.1%), egg roll(9.4%), soysauced beef(8.2%), toasted laver(6.3%), bean sprout namul(3.0%), danmooji(1.0%), and squash namul(0.4%)-was relatively low. It can be interpreted that elementary school student prefers processed food specially animal food to kimchi. The hot taste of the kimchi was the number one reason of their dislike of kimchi, and it was the most important reason for those also like kimchi. Thus it can be thought as the representative taste of kimchi. Chinese cabbage kimchi was found to be the most favorite kimchi and kakdugi(seasoned pickles of cubed radish), nabak-kimchi(mildly seasoned water based kimchi that is mixture of chinese cabbage and cubed radish) and jchonggag kimcchi(seasoned pickles of pony tail radish) and cucumber kimchi were followed in order. Among the various ingredients in kimchim children like cabbage best but they didn't like galic, ginger, green onion and fermented fish sauce which give strong flavor in kimchi.
A Survey on the Children한s Notion in Kimchi(II) - Children한s Opinions for Kimchi and Their Actual Consuming Behavior -
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 765~770
A survey on the children's thought for kimchi intake and practical amount of kimchi consumption were conduction in April of 1995 to get basic information needed for the development of special kimchi for the children. And the effects of nutritional guidance on consumming kimchi was also investigated. Total of 1100 students in 2nd, 4th, and 6th grade from public and private elementary school participated in this survey. The eighty three percent of student think they should eat kimchi mainly because kimchi is good for the health(48.0%) and kimchi is our traditional food(31.8%). However, only 26.5% of children consume more than 5 pieces(
) of kimchi per mela and 64.5% consume less than 1~2 pieces. This shows that children's opinion(p<0.001), preference(p<0.001) and actual consumption(p<0.001) for kimchi was found to be very significant. Thus the nutritional guidance is important as much as the development of children's kimchi. Children wanted kimchi which is sweet, mild hot and not too strong in flavor. Ingredients children wanted in kimchi were pear, apple, tangerine, carrot and onion. Children's most favorite foods cooked with kimchi were stir fried kimchi rice and kimchi stew. But children did not like kimchi hamberger, pizza topped with kimchi and rice cooked with bean sprout and kimchi.
Optimum Production Condition of Oligosaccharide by Bacillus cereus IAM1072
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 771~778
This study was carried out to produce the oligosaccharides(G2~G7) directly from the culture broth of Bacillus cereous IAM1072. The maximal production of oligosaccharides was obtained after cultivation at
for 24hrs in the mixture of 12% soluble starch, 1.5% peptone and 0.25%
adjusted to pH 7.0. Among the oligosaccharides produced
oligosaccharide was purified. After methylation, the result of GC analysis was suggested to be oligosaccharide consisting of 5 glucose unit with
Production of 5균-GMP by Immobilized 5균-GMP Producing Fusant RC102
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 779~784
The effective production of 5'-GMP(5'-Guanylic acid) by immobilized 5'-GMP producing fusant RC102(intergeneric protoplast fusion between Brevibacterium ammoniagenes ATCC21263 and Corynebacterium glutamicum ATCC21171) was investigated. The Fusant RC102 was immobilized by entrapping in -carrageenan, agar, polyacrylamide or Ca-alginate. 3% k-carrageenan was selected as the most suitable matrix. In the production of 5'-GMP using the immobilized whole cells of fusant RC102, the optimum conditions were
, pH 8.0,
. In order to use fermentation medium containing CSL(Corn Steep Liquor) plentiful in
, the optimum conditions of penicillin G, D-cycloserine and POESA(polyoxyethylene stearylamine) for production of 5'-GMP were 0.8unit/ml, 0.8unit/ml, 0.8unit/ml and 5mg/ml, respectively. Cationic surfactant, POESA was effective and superior to the antibiotics, penicillin G or D-cyloserine in 5'-GMP productivity. The condinuous fermentation using immobilized fusant RC102 showed that 5'-GMP productivity was stable for more than 15 days.
Optimization of Batch Expression of Sesame Oil
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 785~789
In order to optimize the batch expression of sesame oil, recovery of expressed oil(REO) from roasted and unroasted sesame seeds were observed at different temperature, pressure, pressing duration and moisture content, and relatinship between REO and effects of expression factors were analysed. REO was high at 2.5~4.5% moisture content, 30~
and 30~50MPa, and decreased abruptly with increasing moisture content above 4.5%. The optimum temperature, pressure, pressing duration and moisture content were
, 54.4MPa, 21.7min and 1.3% for unroasted seeds and
, 37.8MPa, 14.4min and 2.52% for roasted seeds, respectively. REO in optimum condition was 84.6% in unroasted seed and 81.7% in roasted seed. From the statistic analysis between effects of expression factors and REO, importance of their effects was decreased in the order of moisture content, pressure, temperature and pressing duration. And also interaction effects were high in
content and temperature pressure. The multiple regression equation between REO(Y) and temperature(T), pressure(P), moisture content(M), and pressing duration(D) were as follows ;
, for unrosated sesame seed,
for roasted sesame seed.
Isolation of Bioflocculant-Producing Microorganism and Its Culture Characteristics
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 790~795
bioflocculant;flocculation activity;flocculation of solid materials;ArcuadendronFlocculant-producing microorganisms were isolated from soil samples using kaoline as the flocculating test material. One strain that had high flocculating activity among them was selected and identified as Arcuadendron sp. TS-49. The favorable medium for production of the flocculant was 3% glucose, 0.2% yeast extract, 0.1%
, and 0.05%
in 150ml of T.W. with initial pH 7.0.The optimum culture temperature and pH were
and pH 7.0, respectively. The flocculant activity was observed most highly after 4 to 5 days of cultivation at the optimum condition and decreased significantly with the lapse of cultivation time. The flocculant was produced constituently and seemed to be degraded for ressimilation during cultivation. The productivity achieved by this system was about ten-fold higher than that of scrrening mediuim. This bioflocculant flocculated all tested solids, including various microorganisms and organic/inorganic compounds. Several qualitative analyses of the bioflocculant showed that it was a kind of glycoprotein containing sugars and protein.
The Changes in Firmness, Ca Content and Polygalacturonase and Pectinesterase Activities during Oyijangachi Preparation
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 796~802
Oyijangachi, a traditional Korean brinded cucumber, was prepared by brinning the cucumbers in five different solutions for 48 hrs and then, was dipped into dipping sources(Kochujang, Doenjang and Ganjang) for 30 days of aging. Firmness, calcium content and enzyme activities(pectinesterase and polygalacturonase) changes were measured among the cucumbers which were treated by five different solutions during aging. The firmness of Kochujang Oyijangachi were the lowest after 10 days of aging for all from the five brining solutions because of "hollow phenomena" of cucumbers. Calcium contents of cucumbers after dipping into the five solutiosn increased as calcium content of the solutions increased and also increased when the cucumbers dipped into the dipping bases(Kochujang, Doenjang and Ganjang) because of calcium migration from the dipping sources into the cucumbers during aging. The calcium contents of the three dipping bases were ranged from 70mg% to 120mg% of Ca. The activity of polygalacturonase in the Oyijangachi decreased generally during aging and decreased rapidly during initial 5 days of aging. The activity of pectinesterase of cucumbers treated with 12% salts solutions(treatment 3, 4 and 5) were higher than those of cucumbers treated with 6% salts solutions(treatment 1 and 2).
Optimal Conditions of Co-Immobilized Mixed Culture System with Aspergillus awamori and Zymomonas mobilis
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 803~810
Co-immobilized mixed culture system(A-Z system) composed of two different oxygen-demanding strains, aerobic(Aspergillus awamori) and anaerobic(Zymomonas mobilis) strains, in a Ca-alginate gel beads was developed to increase ethanol production from raw starch as a carbon source. Optimal mixture ratio of A. awamori and Z. mobilis was
and 0.5g cells/L-gel, respectively. After 120 hours of cultivation, gel beads distinguished oxygen-rich surface for A. awamori from oxygen-deficient central part for Z. mobilis. At A-Z culture system, yield of ethanol on glucose,
, was very low and there was high leakage of cells from surface of gel beads. At A-Z 36 cultrue system with changing silicon check valve for cotton plug at 36 hours in A-Z culture system, there was no cell leakage from gel beads, pH was maintained at around 4.3 during cultivation, and yield of ethanol on glucose,
, showed 2 times higher than that of control culture system(cotton plug culture).
Effect of Deamidation with Neutrase on the Solubility of BSA, Egg Albumin, and Soy Protein Isolate
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 811~815
Effect of deamidation with Neutrase on the solubility of bovin serum albumin(BSA), egg albumin(EA), soy protein isolate(SPI) was investigated. Solubility of deamidated BSA in distilled water was decreased from 98% to 83% against native BSA at pH 4~8, minimum solubility of deamidated BSA was pH 6. Solubilities of native BSA and deamidated BSA in 0.2M NaCl solution were shown 100% as compared greately decreasing both solubilities in 1.0M NaCl at acidic pH. According to deamidation, solubility of EA in distilled water was increased below pH 4 and above pH 6, while solubility of EA in NaCl solution was decreased by deamidation at acidic pH. Solubility of SPI in distilled water was greately increased by deamidation at overall pH, deamidation was increased solubility in NaCl solution above pH 5. There was, however, no difference on solubility by deamidation below pH 5.
Effects of Heat Treatment on Protein Quality as Lysine Damage
Journal of the Korean Society of Food Science and Nutrition, volume 24, issue 5, 1995, Pages 816~828
During the industrial preparation and the storage of foods, the side chain of some protein-bound amino acids can react chemically each other or with other molecules present in the food. The following reactions have been described : destruction of amino acids, racemization, protein-protein interactions, reactions of proteins with reducing sugars, oxidizing agents, or polyphenols. Apart from total destruction, the main reacitons are the forming of Maillard reactions products(e.g. fructoselysine) and the crosslinking with other amino acids in the same or in another protein molecule(e.g. lysinoalanine). The most often involved amino acid is lysine because of its free functional
acid group. Generally derivatives of amino acids or crosslinks in polypeptides influence the bioavailability and the overall digestibility of the protein. This work reviews the technological, analytical, nutritional, and physiological problems related to the formation of fructoselysine and lysinolalnine in human foods, and evaluates the possible health risk for humans. A summary of the available information is of help in considering whether or not the presence of fructoselysine/lysinoalanine in foods represents a danger to man. The reduction in protein quality through these reactions is not a problem for the general population, but it is extremely important in infant foods, since infants are often nourished with a limited number of food product(e.g. formular foods) which are sensitive to the Mailard reaction.