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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Journal of the Korean Society of Food Science and Nutrition
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The Korean Society of Food Science and Nutrition
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Volume & Issues
Volume 26, Issue 6 - Dec 1997
Volume 26, Issue 5 - Oct 1997
Volume 26, Issue 4 - Aug 1997
Volume 26, Issue 3 - Jun 1997
Volume 26, Issue 2 - Apr 1997
Volume 26, Issue 1 - Feb 1997
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Changes in Soluble Proteins during Softening of Persimmon and Jujube Fruits
Seo, Chi-Hyeong ; Shin, Seung-Ryeul ; Jeung, Yong-Jin ; Kim, Kwang-Soo ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 175~179
Changes of protein contents and chromatogram patterns by gel filtration chromatography was investigated for the purpose of studying changes of Proteins during softening of persimmon and jujube fruits. Contents of water-soluble and salt-soluble proteins were increased during softening of persimmon and jujube fruits, but that of cell wall-bound proteins was decreased. After performing a gel filtration of water-soluble protein, one peak was separated in mature persimmon fruits and three peaks in soft persimmon fruits. In the case of jujube fruits, there were three peaks in both of mature and soft fruits. Pattern of salt-soluble and cell wall-bound proteins by gel filtration chromatography hardly changed during softening. During softening of two fruits, the contents of water- soluble and salt-soluble proteins appeared to be increased on the same fractions with the decreasing in content of cell wall-bound proteins.
Changes in Polygalacturonase during Softening of Persimmon and Jujube Fruits
Seo, Chi-Hyeong ; Shin, Seung-Ryeul ; Jeung, Yong-Jin ; Kim, Kwang-Soo ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 180~185
This work was carried out to investigate change of polygalacturonase activities and polygalacturonase patterns by gel filtration chromatography during softening of persimmon and jujube fruits. During softening of two kinds of fruit, polygalacturonase activities of water-soluble and salt-soluble proteins were increased, but that of cell wall-bound proteins was decreased. In water-soluble and salt-soluble proteins of persimmon fruits, two peaks of polygalacturonase activity were separated in mature stages, but one peak in soft stages. During softening of those fruits, the peaks of polygalacturonase activity in water-soluble and salt-soluble proteins appeared on the same fraction with the peaks of polygalacturonase activity in cell wall-bound proteins.
Modulation of Cellulalr Quinone Reductase Inducibility by Roasting Treatment and Acid Hydrolysis of Perilla
Hong, Eun-Young ; Kang, Hee-Jung ; Kwon, Chong-Suk ; Nam, Young-Jung ; Suh, Myung-Ja ; Kim, Jong-Sang ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 186~192
Increased activities of phase 2 enzymes including quinone reductase(QR) have been reported to be associated with protection of animals from neoplastic, mutagenic, and other toxic effects of many carcinogens. In previous study, we found that methanol extract of roasted and defatted perilla meal induced the activity of quinone reductase, an anticarcinogenic marker enzyme, in murine hepalc1c7 cells. Current study showed that unroasted perilla had a limited QR-inducing activity, suggesting that roasting cause the generation of active component(s). Thus we hypothesized that QR inducer in perilla might be covalently linked to sugar moiety and released during roasting process. Methanol extract of defatted raw perilla was subject to acid treatment in order to hydrolyze the potential sugar moiety. Prolonged hydrolysis of methanol extract of defatted raw perilla at
increased the ability to induce cytosolic QR activity of hepalclc7 cells. Furthermore roasting at 180 and
resulted in significant induction of QR activity. The result strongly support the idea that QR inducer(s) is present in bound form in raw perilla and released during roasting. Cellular QR activity was induced proportionately with the increase of concentration of methanol extract of roasted perilla. The induction of QR by defatted perilla was also examined in the cytosols of liver, small intestine, stomach, lung and kidney of male ICR mice. Induction patterns showed specificity with respect to target tissue and roasting of perilla. Unroasted perilla meal (defatted) significantly induced QR in liver and lung, while roasted perilla meal induced QR in liver and stomach. The observation that raw perilla showed similar QR induction patterns to roasted perilla is consistent with our proposal that QR inducer(s) is present in bound form and released by physical and chemical treatments as digestive or microbial enzymes could release the inducers from inactive glycoside forms in gastrointestinal tract of mice. In conclusion, perilla could exert protective effect against chemically induced carcinogenesis by inducing phase 2 enzymes in biological systems regardless of chemical and physical process such as roasting.
Fractionation of Anticarcinogenic Enzyme Inducer(s) from Roasted Perilla
Hong, Eun-Young ; Kang, Hee-Jung ; Suh, Myung-Ja ; Nam, Young-Jung ; Kwon, Chong-Suk ; Kim, Jong-Sang ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 193~197
Elevation of the activities of phase 2 enzymes such as quinone reductase(QR) provides protection against several types of neoplasia. In this study, we performed partial purification of QR inducer(s) from roasted and defatted perilla meal by solvent fractionation and thin layer chromatography. Cellular QR induction was most notable in chloroform fraction of roasted perilla extract, compared with other solvent fractions. QR inducer(s) was partially purified by TLC, with 0.8 of
value in n-butanol : n-propanol : 2N-ammonium hydroxide(10 : 60 : 30). AHH-inducing activity in TLC fractions isolated from methanol extracts of roasted perilla comigrated with QR-inducing fraction, suggesting that QR and AHH are induced by the same compound. TLC fractions shown strong QR-inducing activity also had a potent antioxidative activity, suggesting that cellular QR enzyme is induced by antioxidant(s) present in roasted perilla.
The Nature of Viscous Polysaccharide Formed Kimchi Added Sucrose
Hahn, Young-Sook ; Woo, Kyung-Ja ; Park, Young-Hee ; Lee, Tae-Young ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 198~202
Jangkimchi is a kind of Kimchi which is made with soy-sauce instead of salt. Occasionally, when sugar is added to Jangkimchi for condiment, the kimchi juice becomes viscous. In this study. the nature of the viscous material and the condition for producing viscous property in Kimchi juice were investigated. HPLC analysis showed that the viscous material in Jangkimchi is polysaccharide composed of glucose. Sucrose was more effective in forming viscous juice than glucose and the viscosity increased with the addition of sucrose up to 10%. Soy-sauce also played a role in increasing the viscosity of Kimchi juice compared with salt. Aerobic fermentation condition was found to be another factor to make the juice viscous. The population of Leuconostoc mesenteroides, well-known producer of viscous dextran was not different in the Kimchi juice prepared with the addition of sucrose from that without sucrose, which implys that the Jangkimchi preparation methods such as addition of sucrose and soy-sauce would do some effects on the production of viscous material in Jangkimchi.
A Study on the Antioxidative Activities of Korean Soybeans
Bae, Eun-A ; Moon, Gap-Soon ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 203~208
To investigate the antioxidative effect of Korean soybeans, four kinds of soybeans(yellow, brown, black and small black bean) were selected and determined their antioxidative activities in vitro by POV method. Dark colored soybeans such as brown, black and small black soybean showed stronger antioxidative effect than pale yellow one. When compared the antioxidative effect of these soybeans in hull and dehulled condition, in dehulled soybeans, they showed the same antioxidative effect in each soybeans, but in soybean hull, dark colored soybeans showed stronger antioxidative effect than yellow soybean hull, which means the pigments of soybeans play important roles in their antioxidative activities. To separate the main components showing antioxidative effect in soybeans, these soybeans were extracted with hexane, methanol and water. The methanol extract showed the strongest antioxidative effect among them, which means the major antioxidative effect materials contained in methanol extract.
Identification of Volatile Essential Oil, and Flavor Characterization and Antibacterial Effect of Fractions from Houttuynia cordata Thunb -I. Identification of Volatile Essential Oil Compounds from Houttuynia cordata Thunb -I. Identification of Volatile Essential Oil Compounds from Huttuynia cordata Thunb-
Kang, Jung-Mi ; Cha, In-Ho ; Lee, Young-Kuen ; Ryu, Hong-Soo ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 209~213
Since Houttuynia cordata is well known as a medicinal herb, due to its antibacterial activity on various microorganisms, present investigation was performed to identify the flavor compounds for volatile essential oil. Volatile essential oil was collected by simultaneous distillation-extraction(SDE), and then the oil components were separated on HP-5 capilliary column
and identified those components by GC-MS. Fifty two compounds were isolated from the volatile essential oil of Houttuynia cordata and forty four were positively identified by GC-MS. The volatile compounds were composed mainly of terpenoids(25 classes), aldehydes(7 classes), alcohols(4 classes), ketones(3 classes), acids(1 class) and miscellaneous compounds(4 classes). Of these, the major compounds were
, decanal, 2-undecanone and geranyl propionate.
Identification of Volatile Essential Oil, and Flavor Characterization and Antibacterial Effect of Fractions from Houttuynia cordata Thunb -II. Flavor Characterization and Antibacterial Effect of Fraction from Houttuynia cordata Thunb by Prep-HPLC-
Kang, Jung-Mi ; Cha, In-Ho ; Lee, Young-Kuen ; Ryu, Hong-Soo ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 214~221
The volatile essential oil isolated from Houttuynia cordata were separated to 11 fractions by Prep-HPLC, of these, a fraction(Fr. 6) which carried the characteristic Houttuynia cordata flavor(fishy) contained 2-undecanone,
, 1-decanol and decanoyl acetaldehyde, as identified by GC-MS. From this observation, it may be inferred that 2-undecanone and decanoyl acetaldehyde could be the compounds which play a crucial role in flavoring of Houttuynia cordata. In test of antibacterial activity of eleven fractions of volatile essential oil from H.C., the growths of nine Gram negative bacteria were inhibited obviously when treated with and Fr. 6 including 2-undecanone,
, 1-decanol and decanoyl acetaldehyde, and Fr. 5 including decanal, endobornylacetate, fenchene and decanoic acid, respectively.
Flavor Compounds in Commercial Toha-jeot
Lee, Jung-Suck ; Joo, Dong-Sik ; Kim, Hun ; Jang, Sung-Min ; Choi, Heung-Gil ; Cho, Soon-Yeong ; Cha, Yong-Jun ; Lee, Eung-Ho ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 222~228
Toha-jeot, a traditional salt-fermented seafood in Korea, was purchased on the market in order to analyze the flavor compounds. Volatile flavor compounds in unfermented and fermented Toha-jeot were compared by vacuum simultaneous steam distillation-solvent extraction/gas chromatography/mass spectrometry. A total of 104 volatile flavor compounds were detected in both samples. Of these, 66 were positively identified, composed of aldehydes(14), ketones(8), alcohols(30), terpenes(20), sulfur-containing compounds(10), aromatic compounds (6), esters(12) and miscellaneous compounds(8). Levels of several other compounds such as aldehydes, terpenes, sulfur-containing compounds and esters decreased with fermentation time, whereas alcohols, ketone and aromatic compounds increased. Particularly, levels of alcohols in fermented Toha-jeot was 21 times higher than those of unfermented one. Major volatile flavor compounds in both samples were composed of terpenes, sulfur-containing compounds, esters and ethanol.
Composition of Amino Acids, Sugars and Minerals of Domestic Wheat Varieties
Kim, Chong-Tai ; Cho, Sung-Ja ; Hwang, Jae-Kwan ; Kim, Chul-Jin ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 229~235
The chemical components of 5 domestic wheats(Kru, Suwon 257, Eunpa, Chokwang and Tapdong), Australian standard white wheat(ASW) and dark northern spring wheat(DNS) were determined in terms of amino acids, sugars and minerals for whole wheat and white wheat flour of 70% milling yield. The protein content of whole Eunpa and Tapdong was approximately 15%, and reduced its content approximately 1% after milling compared to that of whole wheats. The high levels of glutamic acid and proline were observed in domestic wheats. The limiting amino acid of domestic wheat was higher than that of ASW, but lower than that of DNS. Especially, Suwon 257 and Tapdong exhibited the higher limiting amino acid content than other domestic wheats. Glutamic acid and proline content increased, while lysine and threonine was decreased after milling. In general, whole wheat exhibited the higher levels of arabinose and xylose, and the lower level of glucose than flour. The results also showed domestic wheat contained the higher amount of arabinose and xylose than the foreign wheats. Domestic wheat and flour possessed the higher amount of mineral such as P, K, Na and Ca than ASW and DNS, while Al and Si were similar to others.
Screening and Characterization of Anticholesterogenic Substances from Edible Plant Extracts
Park, Jeong-Ro ; Park, Jong-Cheol ; Choi, Seong-Hee ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 236~241
Inhibitory effects of several edible plant extracts against 3-hydroxy-3-methylglutaryl coenzyme A(HMG-CoA) reductase, a rate-limiting enzyme in the biosynthesis of cholesterol, were screened. Inhibition rates of
were observed with hot water extracts of Allium fistulosum, Allium sativum and Cucurbita maxima. Methanol extracts of Aster scaber, Allium sativum, Zingiber officinale, Oenanthe javanica and Angelica keiskei effectively reduced the enzyme activity with inhibition rates of
. The methanol extract of Angelica keiskei was fractionated sequentially with chloroform, ethyl acetate and n-butanol. Of the fractions ethyl acetate fraction showed the highest inhibition against the enzyme.
and hyperoside isolated from the ethyl acetate fraction of Angelica keiskei inhibited the enzyme activity by 65.5% and 14.8%, respectively, at the concentration of
Isolation of Flavone Glycoside from Circium japonicum var ussuriense and Biological Activity on the Cardiovascular System
Lim, Sang-Sun ; Lee, Jong-Ho ; Park, Jong-Cheol ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 242~247
A flavone glycoside was separated from the aerial part of Circium japonicum var ussuriense Kitamura and the effect on cardiovascular system was investigated. The cadiovascular contractility of this compound was observed in the artria and aortae from normal rats. It increased the spontancous beat in right atria and the contractile force in left atria, and caused the contraction of thoracic aortae. For the blood pressure, it had ascending effect. The effective component, which acted on stimulation and contraction in the artria and aortae of rats was fractionated with n-BuOH, separated and identified by column chromatography, UV, IR,
. The chemical structure for this component was determined to be
Activation Mode and Glycosidic Linkage of Anti-Complementary Polysaccharide Isolated from Young Stems of Cinnamomum cassia Blume
Ra, Kyung-Soo ; An, Hyun-Jung ; Kweon, Mee-Hyang ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 248~253
The paper describes the activation mode and the glycosic linkage of anti-complementary polysaccharide isolated from Cinnamomum cassia. The polysaccharide fractions, CC-IIIa, CC-IIIb, and CC-IIIc, activated C3 component existed in normal human serum and produced C3 cleavage segments, C3a and C3b. The polysaccharide, CC-2-IIIa-3 activated the complement system both in the presence and absence of
, suggesting that it involved in both classical and alternative complement pathways. Methylation of CC-2-IIIa-3 was performed with methylsulphinyl carbanion and methyl iodide in DMSO. The methylated products was hydrolyzed, then converted into the partially methylated alditol acetates. Gas chromatography-mass spectrometry(GC-MS) revealed derivatives of terminal
Effects of Kimchi Extracts on the Growth of Sarcoma-180 Cells and Phagocytic Activity of Mice
Choi, Moung-Won ; Kim, Kwang-Hyuk ; Park, Kun-Young ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 254~260
Effects of kimchi extracts on the immune response related to its antitumor activity in vitro and in vivo were investigated. The extracts of kimchi fermented for 0(fresh) and 3 weeks at
showed a direct cytotoxic effect on sarcoma-180 cells, tumor cells in vitro. Methanol extract(4mg/ml), MSF(methanol soluble fraction : 3mg/ml) and hexane extract(fresh : 2.0mg/ml, 3 weeks : 0.3mg/ml) of the kimchi(3 weeks,
) markedly decreased the total numbers of sarcoma-180 cells, but not their viability. The phagocytic activity of peritoneal macrophage of mice was significantly augmented by these extracts of the kimchi compared with that of control in vitro and in vivo. These extracts also raised the phagocytic index, indicating that the number of phagocytized microbes per macrophage increased. Thus, kimchi might show a anti-tumor activity by enhancing the phagocytic cell activities.
Effect of Ascorbic Acid or BHA on the Formation of Cholesterol Oxidation Products during Storage of Salted Mackerel, Scomber japonicus
Kim, Yoon-Sook ; Lee, Il-Sook ; Lee, Joo-Hee ; Sung, Nak-Ju ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 261~269
The autoxidation of cholesterol and lipid was investigated in mackerel during its salting for 50 days. Furthermore, the effects of antioxidants such as ascorbic acid and BHA on their autoxidation were studied. The cholesterol of mackerel during salting was continuously decreased. Its content was quantified by 23.3mg/100g in salted control sample after 50 days and that is only about 33% of total cholesterol content in fresh mackerel. The addition of BHA in mackerel during salting inhibited cholesterol oxidation more effectively than ascorbic acid.7-Ketocholesterol, unique cholesterol oxidation products was detected in this experiment and malonaldehyde, one of lipid oxidation products, contineuosly increased in control sample all the salting days by the almost same pattern but in the additive samples of ascorbic acid or BHA by different patterns, respectively. BHA was more effective antioxidant against cholesterol and lipid autoxidation than ascorbic acid.
Comparison of Dietary Carotenoids Metabolism and Effects to Improve the Body Color of Cultured Fresh-water Fishes and Marine Fishes
Ha, Bong-Seuk ; Kweon, Moon-Jeong ; Park, Mi-Yeon ; Baek, Sung-Han ; Kim, Soo-Young ; Baek, In-Ok ; Kang, Seok-Joong ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 270~284
Effects of dietary carotenoids were investigated on the metaboβsm and body pigmentation of rainbow trout(Salmo gairdneri), masu salmon(Oncorhynchus macrostomos), eel(Anguilla japonica), rock fish(Sebastes inermis) and black rock fish(Sebastes schlegeli). Three weeks later after depletion, these fishes were fed diet supplemented with
, lutein, canthaxanthin', astaxanthin or
for 4 to 5 weeks, respectively. Carotenoids distributed to and changed in integument were analyzed. In the integument of rainbow trout. zeaxanthin,
and canthaxanthin were found to be the major carotenoids, while lutein, isocryptoxanthin and salmoxanthin were the minor carotenoids. In the integument of masu salmon, zeaxanthin was found to be the major carotenoids, while triol, lutein, tunaxanthin,
and canthaxanthin were the minor carotenoids. In the integument of eel,
was found to be the major carotenoids, while lutein, zeaxanthin and
were the minor carotenoids. In the integument of rock fish, zeaxanthin,
and lutein were found to be the major carotenoids, while
and astaxanthin were the minor carotenoids. Likely in the integument of black rock fish,
, astaxanthin and zeaxanthin were found to be the major carotenoids, whereas
, lutein and canthaxanthin were the minor contributor. The efficacy of body pigmentation by the accumulation of carotenoids in the integument of rainbow trout and masu salmon were the most effectively shown in the canthaxanthin group and of eel, rock fish and black rock fish were the most effectively shown in the lutein group. Based on these results in the integument of each fish, dietary carotenoids were presumably biotransformed via oxidative and reductive pathways. In the rainbow trout,
was oxidized to astaxanthin via successively isocryptoxanthin, echinenone and canthaxanthin. Lutein was oxidized to canthaxanthin. Canthaxanthin was reduced to
via isozeaxanthin, and astaxanthin was reduced to zeaxanthin via triol. In the masu salmon,
was oxidized to zeaxanthin. Lutein was reduced to zeaxanthin via tunaxanthin. Canthaxanthin was reduced to zeaxanthin via
. and astaxanthin was reduced to zeaxanthin via triol. In the eel,
and lutein were directly deposited but canthaxanthin was reduced to
, and cholesterol lowering effect by Meju supplementation might be resulted from the modulation of fecal axanthin, astaxanthin and
were oxidized and reduced to tunaxanthin via zeaxanthin. In the black roch fish,
was oxidized to
. Lutein was reduced to
. Canthaxanthin was reduced to
and zeaxanthin. Astaxanthin converted to tunaxanthin via isocryptoxanthin and zeaxanthin, and
was reduced to
Distribution of Insulin-Like Growth Factor-Binding Proteins(IGFBPs) and IGFBP-3 Proteolysis in Noninsulin-Dependent Diabetes Mellitus Serum
Lee, Hwa-Jin ; Kim, Sung-Hyun ; Kwon, Mi-Jin ; Nam, Taek-Jeong ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 285~290
The insulin-like growth factors(IGFs) are bound to several binding proteins(IGFBPs) that appear to regulate IGF transport, receptor binding, and its action. The concentration of these peptides are altered by catabolic conditions. To determine IGF-I and IGFBP levels in noninsulin-dependent diabetes mellitus (NIDDM), sera was obtained from 5 patients and 7 controls. Serum levels of IGF-I in NIDDM were lower than those in either of the controls. By western immunoblot analysis, especially IGFBP-1 levels are increased, whereas IGFBP-3 levels decreased and their fragments was increased in NIDDM serum. IGFBP-3 proteolytic activity in NIDDM sera was inhibited by phenylmethylsulfonylfluoride (PMSF), aprotinin, and ethylenediaminetetraacetic acid(EDTA). This pattern of inhibition was consistent with a metal-dependent serine protease. By gelatin zymography, these proteolytic enzymes were identified as the size of 97 and 69 kDa. IGFBP-1, which is primarily insulin regulated, was increased in NIDDM and may modulate circulating IGF-I levels by regulating capillary passage of IGF-I. IGFBP-3 proteolysis markedly reduces its affinity for the IGFs, particularly for IGF-I. This accelerates their kinetics of dissociation, thereby increasing the proportions of IGF-I in free form and its availability to the cells.
Indirect Assessment of Obesity and Correlation between Energy Intake and Energy Expenditure of Middle School Students
Kim, Yun-Hwa ; Jang, Hyun-Sook ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 291~299
This study was accomplished to investigate the prevalence of obesity in 371 Kumi-city middle school students. The means of this study were anthropometric measurement and questionnaires about nutrient intake and energy expenditure. Anthropometric measurements were height, weight, arm circumference and skinfold thickness(biceps, triceps, subscapular, supraliac, calf). The results obtained are as follows: Energy, calcium, iron intake for boys were lower than RDA. Iron intake was low in girls. Energy intake is lower than energy expenditure and RDA for boys. Energy intake, energy expenditure and RDA for girls were balanced. The average height, weight and arm circumference of boys were respectively 160.0cm, 49.4kg, 23.1cm and those of girls were respectively 155.9cm, 48.9kg, 22.6cm. Prevalence of obesity was respectively 5%, 4%, 7%, 4%, 59%, 15%, 14%, 24% for boys and 6%, 10%, 13%, 9%, 30%, 7%, 1%, 3496 for girls by RBW, BMI, RI, OI, %Fat I, %FatII, %FatIII, triceps. Correlation between subscapular skinfold thickness and BMI, RI, OI and %FatII showed high.
Effect of Aster scaber and Ixeris dentata on Contractility and Vasodilation of Cardiovascula and Endothelial Cell in Hyperlipidemic Rat
Lim, Sang-Sun ; Lee, Jong-Ho ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 300~307
The effects of Aster scaber and Ixeris dentata on cadiovascular system in hyperlipidemic rats were examined. Five groups of thirty Sprague Dawley rats were fed with the diet contained 1% cholesterol, 0.25% sodium cholate, 10% coconut oil and 5% lard(control group) for 4 weeks. Each experimental diet group was added with 5% plant powder or extract of the 5% plant powder by dry weight. Contractile or relaxation responses in the isolated artria and thoracic aortae were measured and the morphological changes of the aortic endotherium from the rats fed the experimental diet were inspected. In response to isoproterenol, the number of right atrial spontaneous beat was significantly lower in Cham chyi powder group
and Sumbagui powder group
than control at
concentration. The contraction forces by injection of phenylephrine and calcium in isolated thoracic aorta were significantly low in each experimental groups compared with the control. The relaxation rates by acetylcholine represented comparatively higher value in
than control. The morphological changes of endothelial cell surface was a little in
compared with control, while the damages were considerably advanced in Cham chyi and Sumgbagui extract diet group
Effects of Angelica keiskei on Lipid Metabolism in Rats
Park, Jeong-Ro ; Park, Seok-Kyu ; Cho, Young-Sook ; Chun, Soon-Sil ; Choi, Seong-Hee ; Park, Jong-Cheol ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 308~313
To investigate the effects of Angelica keiskei Koidz on cholesterol metabolism, male Sprague Dawley rats were fed diets containing 5% of A. keiskei flour for 6 weeks. A. keiskei decreased plasma contents of total cholesterol, LDL-cholesterol and triglyceride while showing no change in HDL-cholesterol, resulting in reduction of atherosclerotic index. Decrease in liver concentration of triglyceride and increases in fecal excretion of cholesterol, total neutral steroid and bile acid were also observed. Those results suggest that A. keiskei improves hypercholesterolemia through, at least in part, reducing the absorption of cholesterol and enhancing the catabolism of cholesterol to bile acids.
Effects of Meju Powder Supplementation on Lipid Metabolism in Rats Fed Hypercholesterolemic Diet
Kim, Jong-Dai ; Lee, Young-Il ; Kim, Bok-Rhan ; Choi, Yong-Soon ; Lee, Sang-Young ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 314~318
This study was performed to examine the effects of Meju supplementation to high cholesterol diet on lipid metabolism in rats. Male Sprague-Dawley rats were divided into two groups(Steamed soybean, Meju) and were fed with 0.5% cholesterol diet. Serum and liver lipid profiles and fecal bile acids excretion were examined after four weeks of experimental diet. Food intake, weight gain and liver weight were not significantly different between the two groups. Total cholesterol concentrations in serum was significantly lower in Meju group compared to steamed soybean group(p＜0.05). HDL-cholesterol, triglyceride, phospholipids and nonestrified fatty acid concentrations in serum were not significantly different between the two groups. Cholesterol, triglyceride, and phospholipids concentrations in liver were not significantly different between the two groups. Fecal bile acid excretion were markedly higher in Meju groups than the group fed steamed soybean(p＜0.05). These results suggest that the cholesterol lowering effect by Meju supplementation might be resulted from the modulation of fecal bile acid excretion in rats.
Effect of Methionine and Selenium Levels on Alcohol Metabolic Enzyme System in Rats
Kim, Myung-Joo ; Park, Eun-Mi ; Lee, Mi-Kyung ; Cho, Soo-Yeal ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 319~326
This study was conducted to investigate the effects of methionine(Met) and selenium(Se) levels on alcohol metabolic enzyme system in rats. Sprague-Dawley male rats were fed on diets containing one of the three levels of Met(0, 3, 9g/kg diet) with or without Se(0.45mg/kg diet). Alcohol was administrated with 25%(v/v) ethanol orally at the same time once a day in alcohol group and isocaloric sucrose was administrated to the control group. The rats were sacrificed after 5 and 10 week of feeding periods. Alcohol dehydrogenase(ADH) and microsomal ethanol oxidizing system(MEOS) activities of hepatic tissuedom were increased more in alcohol treated groups than control group. Increment of activities preinated in simultaneous deficiency of dietary Met and Se(LMet-Se+EtOH) group. Aldehyde dehydrogenase (AIDH) activity was decreased more in alcohol treated groups than control group and significantly decreased in Met and Se supplemented(NMet+Se+EtOH) group. Hepatic cytochrome P-450 content and xanthine oxidase(XO) activity were significantly increased in alcohol treated groups Compared to control group and predominated in Met deficiency(LMet) group and excessive Met administration (HMet) group. Superoxide dismutase(SOD), catalase, glutathione S-transferase(GST) activities tended to increase by alcohol administration, the degree of increase predominated in 10 week. The activity of glutathione peroxidase(GSH-Px) was decreased in alcohol groups and tended to increase in proportion to the level of dietary Met.
Effect of Quercus acutissima CARRUTHERS Extracts on the Lipid Metabolism
Sung, In-Suk ; Kim, Myung-Joo ; Cho, Soo-Yeul ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 327~333
This study was conducted to investigate the effect of Quercus acutissima CARRUTHERS extracts on lipid metabolism. Sprague-Dawley male rats
were fed on containing normal and high fat diets. They were orally administrated(0.02g/100g B.W.) of Quercus aculissima CARRUTHERS ethylacetate-extract and water-extract at the same time once a day respectively. The rats were sacrificed after 6 weeks of feeding periods. In high fat diet group, liver and heart weight were increased but kidney weight was decreased. Contents of total lipid, triglyceride and phospholipid were increased in high fat diet groups. But the degree of increment was reduced by administration of Quercus acutissima CARRUTHERS extracts and water extract was more effective. Significant decrease in serum total lipid content by administration Quercus acutissima CARRUTHERS extracts was not due to decrease of triglyceride content but total cholesterol content. Whereas HDL-cholesterol content was significantly decreased in high fat diet group and improved by administration of Quercus acutissima CARRUTHERS extracts. Total lipid, triglyceride and total cholesterol contents in liver were also increased in high fat diet group but phospholipid content was significantly decreased. The results indicate that Quercus acutissima CARRUTHERS extracts were effective in preventing hyperlipidemia and water extract was more effective.
The Effect of Combined Estrogen/Calcium Therapy on Bone Metabolism in Ovariectomized Rats -III. A Study on Skeletal Composition in Ovariectomized Rats-
Lee, Kyung-Hwa ; Lee, Sung-Sug ; Choi, In-Seon ; Oh, Seung-Ho ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 334~343
This study was performed to elucidate the effects of dietary calcium-salt, estrogen-treatment, and estrogen/calcium treatment on bone metabolism. Ovariectomized rats were used as an animal model. Female Sprague-Dawley rats with a body weight of
underwent ovariectomy or sham-operation. The ovariectomized rats were divided into 9 different experimental groups including saline-treated group, estrogen-treated group, high calcium salt-treated group, and estrogen/calcium treated groups, and fed the experimental diet for 6 weeks. The mineral content, weight, length, strength and density in femur and scapula of the animals were determined. The results of the experiment are as follows: there were no difference in weight of wet bone, density, length, strength and content of ash in right femur between ovariectomized rat and sham-operation. When the weight of bones was expressed as bone gram per body weight, it was significantly lower in ovariectomized rat than sham-operation. Estrogen and estrogen/calcium in ovariectomized rats resulted in increased weight of wet bone. Estrogen with its gradual reduction in ovariectomized rats showed the lowest values in strength, content of ash, content of calcium and content of phosphorus among the groups. There were no differences in weight of wet hone, density, content of ash, content of calcium and content of phosphorus in right scapula between ovariectomized rat and sham-operation. In addition skeletal composition was not changed by ovariectomy. Estrogen and estrogen/calcium in ovariectomized rats resulted in decreased skeletal composition however, estrogen with its gradual reduction did not caused the skeletal composition change. Moreover, the gradual reduction of estrogen had a preventive effect on bone loss when the treatment was combined with calcium intensification.
Effects of Zinc on the Antioxidative Enzymatic System and Metallothionein Synthesis in Streptozotocin-induced Diabetic Rats
Choe, Won-Kyung ; Rhee, Soon-Jae ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 344~350
The present study was carried out to investigate the effects of zinc and vitamin E on the antioxidative defense mechanism in the liver of streptozotocin(STZ)-induced diabetic rats. Levels of blood glucose of STZ-diabetic rats were higher than that of control, but ZDM(
10mg/kg injection+STZ) group was lower than those of DM(STZ injection) and EDM(vitamin E 400mg/kg diet+STZ) group. Levels of plasma insulin were lower in all three STZ-diabetic groups than those of control. Thiobarbituric acid reactive substances(TBARS) peroxide values(LPO) in liver were increased 2.3-fold in DM group compared with those of control, while LPG in ZDM group was lower than that of DM group, and EDM group had similar tendency compared with that of control. Reduced glutathione(GSH) contents of liver were decreased in DM group compared with those of control, but increased 2.3, 1.7-fold in ZDM and EDM groups, respectively, compared with those of DM group. Oxidized glutathione(GSSG) was increased in DM group compared with control and GSSG in ZDM and EDM group were lower than that of DM group. GSH/GSSG ratio had similar tendency compared with results of GSH. The activities of free radical scavenging enzymes such as superoxide dismutase, glutathione peroxidase and glutathione S-transferase were significantly decreased in DM group compared to those of control, but higher in ZDM and EDM groups than those of DM group. The metallothionein contents in liver and kidney were increased in DM and EDM groups were remarkably increased 20, 5.3-fold in ZDM group, compared with those of control.
Purification and Characterization of Cyclodextrin Glycosyltransferase from Bacillus firmus
Sohn, Cheon-Bae ; Kim, Seong-Ai ; Park, Young-A ; Kim, Myung-Hee ; Moon, Sook-Kyung ; Jang, Sun-Ae ; Lee, Myung-Sun ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 351~357
The cyclodextrin glycosyltransferase(EC 22.214.171.124) from Bacillus firmus was purified by precipitating with ammonium sulfate followed by, DEAE-Sephadex A-50 column chromatography and Sephadex G-100 column chromatography. In this way, we were able to obtain the single band protein on SDS-PAGE with a yield of 12%, whose purity was 49 fold. The purified CGTase was identified as a protein having molecular weight of approximately 80,000 dalton and isoelectric point of 9.6. The optimum pH and temperature for the enzyme activity were 8.0 and
, respectively. The enzyme was stable at between pH 5.5 and 9.0 and up to
. After 24hr of enzyme reaction using soluble starch as substrate, the ratio of
production was 0.01 : 2.90 : 1.00, respectively. And this CGTase pro-duced mainly
Dietary Fiber and Cholesterol Metabolism
Kang, Hee-Jung ; Song, Young-Sun ;
Journal of the Korean Society of Food Science and Nutrition, volume 26, issue 2, 1997, Pages 358~369
Hypercholesterolemia is a main risk factor to develop cardiovascular disease, a major cause of death in Korea currently. Dietary factors which shows hypocholesterolemic effect have been reported, which includes plant proteins, unsaturated fatty acids, milk, calcium, flavonoids. Numerous animal and human studies confirmed the pronounced hypocholesterolemic effects of soluble dietary fiber, such as psyllium husk, pectin, sodium alginate, guar gum. Several hypotheses have been proposed to explain the hypocholesterolemic effect of dietary fiber, including binding of bile acids by fiber, interference of lipid absorption and reduced hepatic cholesterol synthesis by propionate, a product of soluble dietary fiber fermentation. Several other hypotheses have been proposed, and these hypotheses are not mutually exclusive.