Go to the main menu
Skip to content
Go to bottom
REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Journal of the Korean Society of Food Science and Nutrition
Journal Basic Information
Journal DOI :
The Korean Society of Food Science and Nutrition
Editor in Chief :
Volume & Issues
Volume 29, Issue 6 - Dec 2000
Volume 29, Issue 5 - Oct 2000
Volume 29, Issue 4 - Aug 2000
Volume 29, Issue 3 - Jun 2000
Volume 29, Issue 2 - Apr 2000
Volume 29, Issue 1 - Feb 2000
Selecting the target year
Analysis of Organic Acid in Korean Apple Juice by High Performance Liquid Chromatography
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 181~187
The contents of organic acid in Korean apple juice were analyzed by HPLC using YMC-peak ODS-AQ column and enzymatic assay. Model apple juices were prepared at the laboratory and commercial apple juices were purchased from the market. Individual organic acid contents were as follows: DL-malic acid 62~402mg%, L-malic acid 48~360mg%, citric acid 1.81~15.74mg%, fumaric acid nd~0.50mg%. Together, these tests gave useful information about the quality and authenticity of a particular apple juice smaple. The presence of D-malic acid was a clear indication of adulteration because this isomer did not occur naturally. Fumaric acid and citric acid levels above trace amounts were also inconsistent with pure apple juice.
Physicochemical Properties of Introduced and Domestic Sesame Seeds
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 188~192
To obtain basic information for the quality evaluation, the introduced or domestic sesame seeds were investigated to measure proximate components (crude protein, ash, mineral and oil) and isolated by preparative HPLC system for lignan contents. Although crude protein contents were the highest in Sudan sesame seeds, lipid, ash and mineral contents were not significantly(p<0.05) different between introduced and domestric sesame seeds. Unsaturated fatty acids such as oleic and linoleic acids were the highest in the domestic sesame seeds. The Mg content of domestic sesame seeds also was larger than that of introduced sesame seeds. A Ca content of domestic sesame seeds, however, was significantly (p<0.05) lower than that of China. Lignan contents, the most important component known as antioxidant, were significantly (p<0.05) higher in domestic sesame seeds than other sesame seeds tested. Our findings suggest that domestic sesame seed has the best quality in terms of the functional components.
Effects of Methanol Extracts of Black Soybean on Enzymes Activities of Serum in Rats Fed Pb and Cd Solution
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 193~197
The effects of black soybena extracts on enzymes activies of rat were evaluated in present study. Eighty-four male Sprague-Dawley rats weighing 100
10g were divided into twelve groups which consisted of black soybean extract, Pb and Cd solution, and black soybean extract plus Pb or Cd soln groups. The weight gain was increased in black soybean extracts and Pb soln solution group but decreased in Cd soln solution group. The results obtained form the experiment were as follows: Glutamate pyruvate trasaminase (GPT) and glutamate oxaloacetate oxaloacetate transaminase (GOT) activities were not significantly different among experimental groups. The lactate dehydrogenase (LDH) activities of black soybean extract administered groups were decreased than those of Pb and Cd solution group. Black soybean group increased cholinesterase (ChEase) activity as compared to administration of Pb and Cd soln group.
Rheological Properties of Biopolymer Produced by Bacillus coagulans CE-74
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 198~203
A highly viscous biopolymer from Bacillus coagulans CE-74 was purified and its rheological properties were studied The rheological properties of biopolymers produced by Bacillus coagulans CE-74 were studied at the temperature ranges with 20~8
, at the concentration of 0.5~4.0%, at the pH ranges from 3 to 1 and at the shear rate fo 7.34~73.38 sec-1. The apparent viscosity of biopolymer was decreased with increasing shear rate, and thereby biopolymer showed pseudoplastic characteristics. Biopolymer solution showed a characteristic of non-Newtonian fluid properties. At the concentration of 1%, the consistency index and the flow behavior index were shown at 2.64 poise. sec11 and 0.8571, respectively. All dispersions were pseudoplastic fluids described accurately by Herschel-Bulkley model. The change of the biopolymer viscosity on pH showed the highest value at the pH 7.0 and it showed lower at acidic conditon that at alkaline condition comparatively.
The Effects of Solvent Fractions of Kimchi on Plasma Lipid Concentration of Rabbit Fed High Cholesterol Diet
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 204~210
The antioxidative effects of solvent fractions of kimchi on LDL oxidatiojn in vitro as well as hypolipidemic effects of these fractions in rabbit fed atherogenic diet were studied. Methanol extract of deffated kimchi was fractionated sequentially with dichloromethane, ethylacetate, butanol and water. All solvent fractions of kimchi inhibited Cu2+-induced LDL oxidation. Among these fractions, the dicholoromethane fraction at the concentration of 25
/mL showed the highest antioxidant effects against LDL oxidation in the aspect of inhibiting TBARS production by 28.03% or prolonged lag phase duration 2-fold compared to those of control. Based on the results from in vitro study, New Zealand White Rabbits grouped six each were fed for 8 weeks either basal diet containing 1% cholesterol or experimental diet containing dichloromethane, ethylacetate or water fraciton added to the basal diet. The amount of solvent fraction of kimchi added to the experimental diet was equivalent to 5% of freeze-dried kimchi. The hypolipidemic effects was observed from all experimental gropus, especially from dichloromethane fraction added group. The plasma and LDL cholesterol levels of this group were decreased by 49% and 47%, respectively while that of HDL increased by 91% compared to those of control. The calculated atherogenic index for the dichloromethane group was the lowest among groups. However, TG lowering effect of experimental group was not observed since solbent fraction of kimchi was used instead of freeze-dried kimchi. The TBARS concentration of LDL isolated from rabbit fed dichloromethane fraction was decreased 21% than that of control. These results indicate that active principles responsible for inhibiting LDL oxidation and lowering plasma cholesterol may present abundantly in dichloromethane fraction of kimchi.
Quality Assessment of Commercial Doenjang Prepared by Traditional Method
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 211~217
To standardize quality characteristics of commercial doenjang prepared by traditional method, general components, organic acids, amino acids and fatty acid composition were investigated. Moisture, crude protein, crude lipid, titratable acidity (expressed as milliliter of 0.1 N NaOH), acid value and salinity were 54.7%, 13.8%, 8.0%, 14.4mL, 45.7mg/g and 11.8%, respectively. The content of amino type nitrogen was 345.3mg% (w/w) in the range of 207.6 to 443.5mg% being 1.1~2.2-fold for all samples. Average value of Hunter color for L(lightness), a(redness) and b(yellowness) were 37.4, ＋9.7 and ＋21.3, respectively. Browning index value(expressed as optical density at 425 nm) of water-soluble compound(2.58) was above 7.1 times higher than that of the water-insoluble compound(0.38). Total contents of free and total amino acid were 3.81 and 9.72%, respectively, and then the former(1.1~3.7 times) was more different in all samples tested than that of the latter(1.1~2.0 times). Among amino acids, glutamic acid was abundant and its content of free and total amino acids was 0.57 and 1.24%, respectively. Of organic acids, lactic acid was the most abundant being 0.34%(average) and ranged from 0.15 to 0.86%. Linoleic acid(52.17%, as area%) was the highest content of the total fatty acids. Unsaturated and polyunsaturated fatty acids were 83.89 and 61.189%, respectively. Polyunsaturated fatty acid was markedly different among all samples and ranged from 51.52 to 64.91%.
Quality Maintenance of Minimally Processed Chinese Cabbage for Kimchi Preparation
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 218~223
The objective of this study was to investigate the effectiveness of preservatives for extending storage life and maintaining the quality of minimally processed Chinese cabbage. Cut Chinese cabbage was treated with either 1% CaCl2, 1% NaCl, 3% sucrose, 1% Ca-lactate, 1% vitamin C, 0.05% chitosan＋1% vitamin C, 0.1% Sporix＋1% vitamin C or hot water(6
) and then packed with polyethylene film(60
) and stored at either 2
. To evaluate biochemical changes and quality of minimally processed Chinese cabbage, the samples were tested to determine the amount of vitamen C, titratable acidity, organic acid and fiber contents. Changes in color were also examined. The quality of kimchi prepared form minimally processed cabbage was affected by the treatments. REsults indicate that the minimally processed Chinese cabbage treated with either 1% CaCl2 at 2
or 1% NaCl at 2
minimized biochemical changes in plant tissue and those treatments were most effective in maintaining product quality. The cabbage treated with 1% vitamin C or 1% NaCl at 4
resulted in kimchi with improved color, texture, flavor and the best overall acceptability, as determined by a sensory test.
Preparation and Quality of Uncooked-Colored Wine Using Black Rice
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 224~230
In order to study the quality of uncooked-colored wine by using polished black rice(PBR) and glucoamylase, the mixture ration of PBR ws investigated. The growths of yeast and lactic acid bacteria, pH and alcohol concentration of the colored wine prepared by adding PBR in the range of 20 to 100% were higher than those of polished rice only, whereas the contents of residual sugars, total free amino acids and fusel oil of the colored wine were lower. The colored wine prepared by 20% PBR and that of over 80% PBR showed a light red color and a dark red, respectively. The optimal addition ratios of PBR evaluated by palatability of color and flavor, and sensory overall quality was 40 to 60%.
Effects of Reusing Times on the Oxidative Stability of Frying Fat for Frozen Battered Pork
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 231~234
To evaluate the effect of frying times on the oxidative stability of soybean oil and shortening, they were reused up to 10 times and measured by thiobarbituric acid(TBA) value, acid value(AV), peroxide value(POV) and sensory evaluation. As the reusing time was increased, TBA value of "battered pork" fried with shortening showed more sharply increased than that with soybean oil, while the value of shortening, itself, showed more decreased than that of soybean oil. In frying fat, AV and POV increased with the increase of reusing times. Compared with soybean oil, shortening had rapidly increased for POV as reusing time was increased. Among the sensory analyses TBA of "battered pork" with soybean oil and shortening exhibited the highest correlation with flavor and texture, respectively. For the "batgtered pork" fried with soybean oil and shortening, reusing shelf-lives predicted were approximately 9 and 8 times, respectively.
Physiological Effects and Utilization of Corbicula elatior Products -Effect of Cockle Extracts on Carcinogen-induced Cytotoxicity and Immune REsponse RElated to Its Antitumor Activity-
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 235~240
Inhibitory effects of cockle extracts on carcinogen-induced cytotoxicity in C3H/10T1/2 cells were studied. Soup (62
/mL), solubility (28
/mL) and liposolubility (9
/mL) of the cockle inhibited 3-methyl-cholanthrene(MCA)-induced cytotoxicity in C3H/10T1/2 cells by 53 and 94%, respectively. These results suggest that the extracts cockle might have anticarcinogen-induced cytotoxicity of C3H/10T1/2 cells. The effects of cockle extracts on the immune response related to its antitumor activity in vitro and in vivo were investigated. The cockle extracts showed a direct cytotoxic effect on sarcoma-180 cells, tumor cells in vitro. Soup (0.49 mg/mL), solubility (0.11 mg/mL) and liposolubiliy (0.05 mg/mL) of the cockle markedly decreased the total numbers of sarcoma-180 cells, but not their viability. The phagocytic acitivity of peritoneal macrophage of mice was significantly augmented by these extracts of the cockle compared with that of control in vivo. These extracts also raised the phagocuytic index, indicating that the number of phagocytize dmicrobes per macrophage increased. Thus, cockle extracts might show a antitumor activity by enhancing the phagocytic cell activities.
Study on Bread-Making Quality with Mixture of Buckwheat-Wheat Flour
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 241~247
The possibility of buckwheat-wheat flour mixture as bread was studied by adding 10, 20, and 30% of buckwheat to wheat flour. To improve bread-making quality of the mixture, gluten, ascorbic acid, and hydroxy propyl methyl cellulose (HPMC) were added to 30% buckwheat-wheat flour and sensory evaluation was also exercised on it. The weight of bread increased but the volume of that decreased as the percentage of buckwheat to wheat flour increased, and improved bread-making quality by adding gluten, ascorbic acid, HPMC to 30% buckwheat-wheat composite flour. When it comes to color, color of the bread got darker as the percentage of buckwheat increased, got brighter when gluten and HPMC were added and showed little difference when ascorbic acid was added. Texture of bread increased in maximum weight, strength, hardness but decreased in springiness and cohesiveness. The use of additives showed influence to the some results. In terms of the aging process, enthalpy increased with storage time, and it could be slowed down by the use of the additives. The sensory evaluation shwoed that 10% buckwheat-wheat bread was most excellent, 20% bread was similar to other bread made from wheat flour, and the bread made by mixing additives were better than just 30% buckwheat-wheat flour in terms of quality.
Effect of Packaging Films on Quality of 의Chuichung의 Cucumbers during Storage
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 248~251
To investigate the effects of packaging films (functional MA films/FC30, FC50, MA film/0.03 mm LDPE, corrugated paperboard box/control) weight loss, firmness, total ascorbic acid, chlorophill and overall appearence were evaluated. Weight loss of cucumbers packed with FC30 and FC50 at 2
after 7 days were 1.0 and 0.6%, that of the control was 2.9%, those of LDPE was 0.5%. The firmness of FC30 and FC50 at 2
after 7 days were 1.0 and 0.6%, that of the control was 2.9%, those of LDPE was 0.5%. The firmness of FC30-, FC50 and LD was higher than that of the control. Total ascorbic acid contents (TAA) of cucumbers packed with control, LD, FC30 and FC50 box were 9.96, 10.86, 11.37 and 11.29 mg% after 7 days. TAA of FC box was 10% higher than that of the control. Chlorophill content of FC boxes wase 16% higher than of the control. Overall appearence of cucumber packed with FC boxes was better than that of the control.
Effects of Extruded Job s-tear Cereal on Lipid Metabolism in High Fat Fed Rats
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 252~256
Coronary heart disease (CHD) is caused by elevated blood lipids and the death rate from CHD is continuously increasing in recent years. In Korea, death rate from CHD is more than 30%. Recently, it has been reported that Job's tear decreaseds blood cholesterol level. However, most of the reports used Job's tear itself. Therefore, we have examined the effect of extruded Job's-tear cereal on blood and liver lipid levels in rats fed with a high fat diet for 5 weeks. The energy intake and body weight gain were higher in Job's tear cereal group (JFD) than in high fat fed group(HFD). Blood triglyceride concentration was significantly reduced in JFD group (p<0.05). And Atherogenic Index was decreased about 20% in JFD suggesting favorable effect of Job's-tear cereal on hyperlipidemia. Liver lipids levels were not affected by Job's-tear cereal.
A Comparative Study of Nutrient Intakes and Factors to Influence on Nutrient Intake between Low-income Elderly Living in Urban and Rural Areas
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 257~267
This study was carried out to compare the nutrients intakes, factors to influence on nutrient intake and frequency of food group intake between the two gruops of elderly living in Yangcheon, Seoul(n=105) and in Yongin(n=159). The results obtained by cross-sectional survey using questionnaires were as follows. 1) The average age of the subjects was 71.9 and there was no significant difference in age and gender distribution according to the residential areas. 2) The intakes of energy, protein, Fe, vitamin A, niacin, thiamin, riboflavin, and vitamin C were significantly higher in the urban elderly than those of the rural elderly(p<0.05).3) The average daily intakes of energy, protein, Ca and Fe of the subjects were lower than those of the Korean RDAs in general and the rural elderly showed more severe deficiencies. 4) Individual nutrient intakes and food group intakes were related to the pocket money, number of different foods taken daily, education level, residential area, and the degree of exercise. These results suggested the needs of nutrition intervention for the low-income, rural elderly.
Effect of the Ethanol Extract of Lycium chinense on the Oxygen Free Radical and Alcohol Metabolizing Enzyme Activities in Rats
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 268~273
To investigate an effect of the ethanol extract of Lycium chinense(EELC) on the activities of enzymes scavenging oxygen free radicals or detoxicating alcohol. The ground Lycium chinense was extracted with 30% edible ethanol and then diluted with 6% ethanol to contain 2% EELC(w/v). Three different groups of male Sprague-Dawley rats had taken a drink EELC, ethanol(ETH) or water(control), respectively for 2 months. At the end of experimental period, the animals were sacrificed and obtained the following findings. The EELC-treated animals showed the highest activity of hepatic glucose-6-phosphatase among three groups. The activities of xanthine oxidase and cytochrome p-450 from EELC treatment group were lower than those from ETH-treated group. However, the activity of superoxide dismutase was higher in the EELC-treated group than the ETH-treated(p<0.005). Furthermore, hepatic alcohol or aldehyde dehydrogenase activity, and glutathione content and glutathione peroxidase were significantly higher in EELC-treated animals than in ETH-treated those. The activity of glutathione S-transferase in liver was appeared the orderly higher value in EELC, ETH and control-treated group. As the result, EELC may affect the reduction of oxygen free radical production and help the detoxication of ethanol.
Effect of Potato Polyuphenolics on the Hyperlipidemia in Rats
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 274~279
Effect of potato polyphenolic compound on the concentrations of serum and liver lipids, serum glucose, and urine protein was investigated in Sprague Dawley rats by feeding a diet containing 0.5% of cholesterol for 2 weeks. Polyphenolic compound extracted from potato (Solanum tuberosum variety Dejima) was supplemented at a 0.5% level in the basal diet or the cholesterol diet. The supplementation of potato polyphenolic compounds decreased slightly the concentrations of total cholesterol and VLDL+LDL-cholesterol in serum, and atherosclerotic index in rats fed the cholesterol diet, while those measurements were not altered by the supplementation of potato polyphenolic compounds in rats fed the basal diet. In the rats fed the basal diet or the cholesterol diet containing potato polyphenolic compounds, urine protein increased by 12% and 27% at 1st week, respectively, but this change was not seen at 2nd week. The concentration of serum glucose, however, was not significantly different in the dietary groups.
In vitro Screening of Dietary Factors on Buckwheat(Fagopyrum esculentum Moench) Influencing the Regulation of Blood Pressure, Glucose and Cholesterol Level
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 280~287
Generally, buckwheat has been regarded as a crop of secondary importance in many countries. In vitro functionalities of buckwheats as a food were evaluated in this study. Five of buckwheat cultivars were extracted with methanol, and the extractant were dried and lyophilized, separately. Or water soluble buckwheat components were digested with the commercial enzymes and the obtained protein hydrolysate was again fractionated by acid precipitation. The antioxidant capacity of the methanol extracts determined using Fe2+-ascorbic acid system was dependent ont the cultivars: The extract of Suwon 4 showed 3.3 times stronger activity than ascorbic acid in terms of IC50. Also, the extracts of buckwheats inhibited efficiently the activities of
-amylase and lens aldose reductase. Buckwheat soluble protein or rutin suppressed the in vitro activities of angiotensin-converting enzyme, and the inhibitory degree depended largely on the cultivars. Buckwheat proteins exerted higher hydrophobicity being related to the sterol binding capacity than casein. The results suggested that buckwheat seeds may be desirable and functional food resources in human living in current society.
Effect of Crude Saponin Fraction from Korean Red Ginseng on Physiological Events of Ovariectomized Rat
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 288~293
This study was carried out to investigate the effect of crude saponin fracton from Korean red ginseng on physiological events of ovariectomized rats. The saponin fractions was prepared by Diaion HP-20 adsorption chromatography and spirit. Total 50 rats were divided into 5 groups : normal control (NC), sham-ovariectomized (SO), ovariectomized (OR), ovariectomized and saponin treated (OS), and normal control treated with saponin (NS). Saponin was intraperitonally administered for 8 weeks. Ovariectomy was carried out at 1 st and 2nd weeks of experiment. Right-side ovary of rat was removed at 1st week, the otehr was done at 2nd week. The body weights fo ovariectomized rats showed no significant changes but that of NS group showed significant increase (p<0.05) compared with NC group. Ovariectomy of rats reduced the weights of femur by 6 to 8% compared with that of NC group. In addition, femur weight of NS group was 5 to 6% higher than that of NC. Uterus weight showed no significant differences by saponin treatment or ovariectomy. Serum testosterone level of ovariectomized rats increased by 60 times compared with that of NC. However, administration of crude saponin to ovariectomized rat attenuated testosterone level to almost that of NC. These results suggest that Korean red ginseng saponin attenuates physiological disorders induced by malfunction of ovary.
The Effects of Hot Water Soluble Polysaccharides from Lentinus edodes on Lipid Metabolism in the Rats Fed Butter Yellow
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 294~299
The effects of hot water soluble polysaccharides extract(PS) of Lentinus edodes on the lipid components in the liver and plasma of the rats fed the butter yellow were investigated. Twenty four rats were divided into four groups and fed with the diet containing 15% casein, 30% sucrose and 10% soybean oil (basal diet; NO group), supplemented with butter yellow (BO group) or/and PS (NP, BP group). The contents of triglyceride and phospholipid in liver were significantly lower in BP group than BO group but the content of total lipid and total cholesterol were not significant difference between BO and BP. The concentrations of total cholesterol and triglyceride of plasma were significantly lower in BP than BO. AS the results PS of Lentinus edodes prevent the triglyceride and cholesterol rise and then improve the lipid metabolism of rats fed the butter yeollow.
Evaluation on the Safety of
-Irradiated Angelica gigas Nakai: Stability of Active Components and Safety in Genotoxicity Test
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 300~306
In the present studies, we assessed the stability of active components and toxicological safety of irradiated Angelica gigas Nakai(Danggui). In order to confirm the stability of active components in the
-irradiated roots of Danggui, the quantitative analysis of decursin and decursinol angelate of
-irradiated sample was carried out by high performance liquid chromatographic (HPLC) methods using reverse phase columns and normal phase columns. From the root of Danggui, decursin and decursinol angelate were isolated by a silica gel column chromatography(toluene : ether (1 : 1), Hexane : EtOAc(15 : 1)). And then the structures were confirmed in the 1H and 13C-NMR analysis. The HPLC chromatograms of decursin and decursinol angelate in
-irradiated Danggui were similar with those of non-irradiated sample. In the examination of in vitro genotoxicity of the water extract from
-irradiated Danggui using Salmonella reversion assay(Ames test) and micronucleus test in Chinese hamster ovary (CHO) cells, mutagenicity was not exhibited in the two assays with or without metabolic activation. These resutls suggest that active components in the
-irradiated Danggui should be stable and that the safety of
-irradiated Danggui could be revealed in further test in vivo.
Screening of Immune-Enhancing Substance(s) from Korean Wheats
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 307~311
The purpose of this study was to identify excellent immune-enhancing substance from Korean wheats(Eunpa, Gueru, Alchan, Topdong, Suwon 267, Gobun) compared with imported ones(Australian standard white, ASW; Dark northern spring, DNS). Phagocytic activities of PBS (phosphate buffered saline, pH 7.4) and EA(ethanol-acetic acid) extracts from the wheats were determined using mouse macrophage J774 cell line. In order to set the optimal experimental condition up, the cultured cells were tested in varying experimental conditions. About two to five times higher phagocytic activity was shown in EA extract of Korean wheats compared to that of imported wheats. PBS extracts of wheats did not show increased phagocytic activity compared to control that did not add any extract. The EA extract of Gobun wheat showed the highest phagocytic activity. From the experiment we found that the optimal experimental condition was shown in two hours of reaction time and 0.05mg amout of EA extract added to J774 cells.
Purification of Macrophage Phagocytic Activity-Enhancing Component from Ethanol-Acetic Acid Extract of Korean Wheat
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 312~315
To purify the macrophage phagocytic activity-enhancing component, ethanol-acetic acid (100 : 1, EA) extract of Korean wheat (Gobun wheat) and imported one (Australian Standard White, ASW) were fractionated with ethylacetate : methanol :
(65 : 25 : 4, v/v/v), and identified by TLC and column chromatography. At least five fractions were separated from the EA extract of the wheats but amounts of fraction B, C and D were more in Gobun wheat than in ASW. The effects of all fractions on phagocytic activity were tested in macrophage J774 cells. Among the fractions, only fraction b of Gobun wheat showed significant increase of phagocytic activity against yeast.
The Antigenotoxic Effects of Korean Native Fermented Food, Baechu Kimchi Using Comet Assay
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 316~321
This study carried out to eluciate the cancer chemoprevention of Korean native fermented food, baechu kimchi using Comet assay (in other words, single cell microgel electrophoresis). For this purpose, baechu kimchi was fractionated by water, n-hexane, chloroform and ethyl acetate. 5 strains of dominant fermented bacteria were isolated from baechu kimchi. The water fraction, n-hexane fraction, chloroform fraction, ethyl acetate fraction and water insoluble fractions showed no antigenotoxicitie in non-tumoral normal 3T3 cells. Among 5 bacteria isolates from baechu kimchi, two isolates bacteria 1 and 2 strongly inhibited genotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in non-tumoral normal 3T3 cells (p<0.05). Bacteria 3, 4 and 5 were also not antigenotoxic.
Antimutagenic and Cytotoxicity Effects of Phellinus linteus Extracts
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 322~328
This study was performed to determine the antimutagenic and cytotoxic effect of the Phellinus linteus methanol extract on Salmonella typhimurium TA98, TA100 and human cancer cell lines. In the Ames test, methanol extract of P. linteus alone did not exhibit any mutagenicity but showed substantial inhibitory effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), 4-nitroquinoline-nitroquinoline-1-oxide(4NQO), 3-amino-1,4-dimethyl-5H-pyrdo[4,3-blindol(Trp-P-1) and benzo(α)pyrene(B(α)P). The methanol extracts of P. linteus(200㎍/plate) showed approximately 78.3%, 78.7% and 88.1% inhibitory effect on the mutagenesis induced by 4NQO, Trp-P-1 and B(α)P. The anticancer effects of P. linteus extract against human breast adenocarcinoma(MCF7), human lung carcinoma (A549), human fibrosarcoma (HT1080), human hepatocelular carcinoma (Hep3B) and human epitheloid carcinoma (HeLa) were investigated. The treatment of 1mg/mL P. linteus extracts had the highest cytotoxicity against MCF7 (92.0%), followed by Hep3B (84.9%), A549 (84.2%) and HT1080 (82.9%). In contrast 1mg/mL treatment of P. linteus extracts had only 10∼40% cytotoxicity on normal human liver cell (WRL68).
Saikosaponin Contents and cAMP Phosphodiesterase Inhibitory Activities in Bupleuri Radix Extracts According to Extraction Conditions
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 329~334
Two major oleananesaponins in Bupleuri radix, saikosaponin
and d, were extracted at various solvent compositions and leaching temperatures. Solvent composition was varied at 0 to 100%(v/v) of ethanol-water and methanol-water, whereas leaching temperature was adjusted to te range of 25~45
. The most effective extractant and leaching temperature were 70% ethanol and 45
, respectively. However, no major differences in saikosaponin content and cAMP phosphodiesterase inhibition were found at various leaching times. The cAMP phosphodiesterase inhibitions were alo the highest when 70% ethanol was used as the extractant.
Screening of Anticoagulant Activities in Extracts from Edible Herbs
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 335~341
In order to search for blood anticoagulant substance from herbal extracts, we extracted with cold water (Fr. I), methanol (Fr. II) and hot water (Fr. III) from 93 commercially available herbs. The anticoagulant activity of herbal extracts was examined through the intrinsic pathway by activated partial thromboplastin time (APTT), the extriansic pathway by prothrombin time (PT) and the common pathway by thrombin time (TT). Onion, garlic, clove, fenugreek, mugwort, thyme and sage had on anticoagulant activity with intrinsic pathway and mint, italian seasoning, rosemary, turmeric, tarragon and wasabie had on anticoagulant activity with common pathway. Nobody had on anticogulant activity with extrinsic pathway. Among commerical herbs tested, clove cultivated in holland was selected because it showed the most potent anticoagulant activity among the samples investigated.
Induction of Nitric Oxide and TNF-
by Herbal Plant Extracts in Mouse Macrophages
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 342~348
In this experiment, we show the effects of herbal plant extracts on the production of nitric oxide (NO) and TNF-
. The extracts of Angelica gigas, Astragalus membranaceus, Acanthopanax sessiliflorus and Houttuynia cordata had no effect on NO synthesis by itself in mouse macrophage cell line (RAW264.7). However, the stimulation with these extracts in the presence of murine interferon-
) resulted in increased NO synthesis. When these extracts were used in combination with mIFN-
, there were a marked cooperative induction of NO and TNF-
synthesis in a dose-dependent manner. The same results were obtained in the mouse peritoneal macrophages used. The optimal concentration of these extracts on NO synthesis was shown at 100
/mL with 100U/mL of mIFN-
. NO synthesis was inhibited by NG-monomethyl-L-arginine. When cell lines were treated with extracts, the expression of inducible NO synthetase (iNOS) was markedly increased in RT-PCR analysis. In addition, synergy between mIFN-
and extracts was dependent on extracts-induced tumor necrosis factor-
). These results suggest that water extracts of herbal plants can induce iNOS, NO and TNF-
synthesis of mouse macrophage cell line (RAW264.7) and peritoneal macrophages in combination with mIFN-
Physiological Activity and Antioxidative Effect of Methanol Extract from Onion (Allium cepa L.)
Journal of the Korean Society of Food Science and Nutrition, volume 29, issue 2, 2000, Pages 349~355
Onion was extracted by methyl alcohol and some physiological activities and antioxidative effect of the extracts on soybean oil, corn oil, lard and shortening were compared with commercial antioxidants such as
-tocopherol and BHT. The electron donating ability was about 70% at concentration above of 30mg/mL. Nitrite scavenging effects of all concentrations diminished at higher pH, while in the case of pH 1.2, it showed a nitrite scavenging effect of more than 80% af concentration above of 30mg/mL. And also, the angiotensin I-converting enzyme inhibitory effect was good above 80%. The soybean oil, corn oil, lard and shortening containing different levels of the onion extract were stored at 6
to evaluate the antioxidative activity of onion extracts. Peroxide value, acid value and TBA value of each oil were detected. Onion extract was very effective to retard oxidation of soybean oil, corn oil and lard, and especially, the very high antioxidative effect was observed for lard.