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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Research
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Journal DOI :
The Korean Society of Veterinary Science
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Volume & Issues
Volume 48, Issue 3 - Dec 2008
Volume 48, Issue 4 - Dec 2008
Volume 48, Issue 2 - Aug 2008
Volume 48, Issue 1 - 00 2008
Selecting the target year
Generation and characterization of calmodulin-DHFR sandwich fusion protein
Han, Chang Hoon ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 243~250
A calmodulin-dihydrofolate reductase (DHFR) sandwich fusion protein was generated by insertion of calmodulin into the
-bulge region of DHFR to observe the effects of structurally constraining the calmodulin structure. The calcium binding properties of the sandwich protein were almost identical to calmodulin. Similar to calmodulin (
), the sandwich protein bound four equivalents of calcium, with half saturation (
) observed at a [
. However, nicotinamide adenine dinucleotide (NAD) kinase activation property of the sandwich protein was lower than that of calmodulin. The sandwich protein activated NAD kinase, but to only half of the level obtained with calmodulin. The K 0.5 for both calmodulin and the sandwich protein were approximately the same (1-2 nM). Methylation analyses of the sandwich protein show that insertion of calmodulin into DHFR results in a large decrease in methylation. The
observed with the sandwich protein (95 nmole/min/ml) was only 22% of the value observed with calmodulin (436 nmol/min/ml) in the presence of calcium. Addition of trimethoprim to the reaction significantly inhibited the observed methylation rate. Overall, the data suggest that the insertion of calmodulin into the DHFR structure has little effect on calcium binding by the individual lobes of calmodulin, but may constrain the lobes in a manner that results in altered interaction with the calmodulin-dependent proteins, and severely perturbed the methyltransferase recognition site.
Loss of cholinergic innervations in rat hippocampus by intracerebral injection of C-terminal fragment of amyloid precursor protein
Han, Chang-Hoon ; Lee, Young Jae ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 251~258
The neurotoxicity of C-terminal fragments of amyloid precusor protein (CT) is known to play some roles in Alzheimer's disease progression. In this study, we investigated the effects of the recombinant C-terminal 105 amino acid fragment of amyloid precusor protein (CT105) on cholinergic function using CT105-injected rat. To study the effects of CT105 on septohippocampal pathway, choline acetyltransferase (ChAT) positive neurons were examined in the medial septum and in the diagonal band after an injection of CT105 peptide into the lateral ventricle. Immunohistological analysis revealed that the number of ChAT-immunopositive cells decreased significantly in both medial septum and diagonal band. In addition, CT105 decreased ChAT-immunopositive cells in the hippocampal area, particulary in the dentate gyros. To study the effect of amyloid beta peptide (
) and CT105 on the cholinergic system, each peptide was injected into the left lateral ventricle, and acetylcholine (ACh) levels were monitored in hippocampus. ACh level in the hippocampal area was reduced to 60% of control level in
-treated group, and the level was reduced to 15% of control level in CT105-treated group, at one week after the injection. ACh level was further reduced to 35% of control in
-treated group, whereas the level was slightly increased to 30% of control in CT105-treated group at 4 weeks after the injection. Taken together, the results in the present study suggest that CT105 impairs the septohippocampal pathway by reducing acetylcholine synthesis and release, which results in damage of learning and memory.
Survey of extended-spectrum β-Lactamase (ESBL) in pathogenic Escherichia coli isolated from poultry in Korea
Sung, Myung-Suk ; Kim, Jin-Hyun ; Cho, Jae-Keun ; Seol, Sung-Yong ; Kim, Ki-Seuk ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 259~265
This study was conducted to investigate incidence of extended-spectrum
-lactamase (ESBL) producing strains and characteristics of ESBL gene in pathogenic Escherichia coli isolated from poultry during the period from April 2003 to December 2005 in Korea. Among 203 isolates, 4 isolates (3 from broilers and 1 from layer) were confirmed as ESBL producing strains by double disk synergy test, polymerase chain reaction and sequencing for
were detected in these 4 isolates and were transferred to recipient by conjugation, respectively. Also, these ESBL producing strains were associated with multiple drug resistance. In conclusion, these results exhibit incidence of CTX-M and CMY-2
-lactamase in pathogenic E coli from poultry in Korea, and clinically important meaning in human. And they also suggest the needs for rapid and broad surveillance to monitor ESBL genes and R plasmid transferring resistant gene in poultry.
A comparison of single dose efficacy of Mycoplasma hyopneumoniae bacterin in swine farms with different serological patterns of PRRSV and PCV2
Kim, Hye Kwon ; Moon, Hyoung Joon ; Kim, Eun Mi ; Yang, Jeong Sun ; Pakr, Seong Jun ; Luo, Yuzi ; Lee, Chul Seung ; Song, Dae Sub ; Kang, Bo Kyu ; Lee, Jaebum ; Park, Bong Kyun ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 267~274
This study was to evaluate the efficacy of single dose Mycoplasma hyopneumoniae (M. hyo)-vaccination in the swine farms which had different serological patterns of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2). A minimum of 240 pigs from each farm was applied, allocating M. hyo vaccinated and control groups. The PRRSV and PCV2 infections were analyzed by serological method (commercial ELISA kit). After administrating pigs a single dose of M. hyo vaccine or control saline at 3 weeks of age, serum antibodies to M. hyo, PRRSV and PCV2 were monitored at 4, 10, 16 and 22 weeks of age. Mortality, weight changes, feed conversion ratio (FCR) and lung score were also evaluated. A single-dose vaccination of M. hyo bacterin was efficacious to reduce mycoplasmal lung lesions and induce good humoral immune response. However, FCR was improved only in one of the three farms where showed seronegative status to both PRRSV and PCV2 in the period from 4 to 16 weeks of age. These results might imply that M. hyo vaccine alone could not overcome the PRRSV and PCV2 infection-associated wasting in the field condition. Therefore, the control of PRRSV and PCV2 should be considered to obtain the better effects of M. hyo vaccination.
Antimicrobial resistance and transfer of R plasmid of pathogenic Eseherichia coli isolated from poultry in Korea
Sung, Myung-Suk ; Kim, Jin-Hyun ; Cho, Jae-Keun ; Seol, Sung-Yong ; Kim, Ki-Seuk ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 275~285
Antimicrobial drugs are widely used in poultry industry as growth promoters or to control infectious diseases. However, this practice is reported to have caused high resistance to antimicrobial drugs in normal chicken flora and pathogens. Antimicrobial resistance to Escherichia coli (E. coli) from chicken has been mainly reported in normal flora, but rare in pathogenic organism in Korea, recently. Therefore, this study was conducted to investigate prevalence of antimicrobials resistance, transfer of R plasmid, and association between antimicrobial drug resistance and O serotype of 203 pathogenic E. coli from poultry in Korea during the period from April 2003 to December 2005. These isolates showed a high resistance to tetracycline (Tc, 93.6%), nalidixic acid (Na, 92.6%), streptomycin (Sm, 81.8%), ampicillin (Ap, 77.3%), ciprofloxacin (Ci, 70.9%), sulfisoxazole (Su, 66.5%), and trimethoprim (Tp, 58.1%). Two hundred-one (99.0%) of the isolates were resistant to one or more drugs. They showed 57 different resistant patterns, and the most prevalent resistant pattern among them was Tc, Sin, Su, Ap, Tp, Ci, Na. Sixty-eight (33.8%) of the isolates transferred all or a part of their antimicrobial resistant pattern to the recipient strain by R plasmid. The most common antimicrobial resistant pattern was Tc, Sm, Su, Ap, Tp, Ci, Na in serotype O78, O88 and O15, respectively. These results exhibit high individual and multiple resistance to antimicrobials of pathogenic E. coli from poultry in Korea. They also suggest the needs for surveillance to monitor antimicrobial resistance in pathogenic bacteria that can be potentially transmitted to humans from food animals and to regulate the abuse of antimicrobials on food-producing animals in Korea.
Genogroup position of aquabirnavirus GC-1 isolated from rockfish Sebastes schiegeli in Korea
Joh, Seong-Joon ; Lee, Youn-Jeong ; Song, Chang-Sun ; Kang, Shien-Young ; Mo, In-Pil ; Heo, Gang-Jun ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 287~293
The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.
Antimicrobial resistance and distribution of tetracycline resistance determinants in Escherichia coli isolated from aquatic birds
Cho, Jae-Keun ; Lee, Sang-Min ; Kim, Ki-Seuk ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 295~303
One hundred and sixty nine Escherichia (E.) coli strains isolated from fecal samples of aquatic birds in Geumho river basin and Dalseong park were tested by agar dilution method to determine their susceptibility patterns to 14 antimicrobial agents. The distribution of tetracycline resistance determinants (tetA, tetB, tetC, tetD and tetE) were also examined by PCR in 76 tetracycline-resistant (
) E. coli isolates. The high resistance was observed in tetracycline, cephalothin and ampicillin (45.0~36.7%). Resistance of E. coli isolates derived from Dalseong park to tetracycline, cephalothin, ampicillin and streptomycin (65.7~44.8%) were significantly higher than those isolated from Geumho river basin (31.4~14.7%). About seventy percent (70.4%) of the strains isolated were resistant to one or more drugs tested. Thirty (39.5%) of 76
E. coli isolates which were resistant to one or more drugs transferred all or a part of their resistance patterns to the recipient strain of E.coli J53 by conjugation. All of
E. coli isolates contained at least one or more of 5 tet genes examined. The most common genes found in these isolates were tetA (60.6%) and followed by tetB (7.9%) and tetC (1.3%). However, tetD and tetE were not found in any of the isolates tested. Twenty one (27.6%) of
E. coli isolates had two determinants, tetA/tetB (20 strains), tetA/tetC (1 strain). And two strains (2.6%) contained three determinants (tetA/tetB/tetC).
A case of transitional carcinoma in the nasal cavity of a dog
Kang, Hwa-Jung ; Kang, Sang-Chul ; Jung, Ji-Youl ; Jo, Suk-Hee ; Roh, In-Soon ; Lee, Joo-Myung ; Cheong, Jongtae ; Kim, Jung-Hun ; An, Min-Chan ; Bae, Jong-Hee ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 305~310
A 10-year-old female Yorkshire terrier with the clinical signs of nasal swelling, epistaxis and nasal discharge was presented to the Veterinary Teaching Hospital in the Cheju National University. Abnormal nasal mass was detected in physical examination and radiographic findings. After surgical excision, the sample of nasal mass was referred to Pathology Department of Veterinary Medicine. Grossly, the mass was soft, friable, and
cm in size. Histopathologically, the mass was composed of mediumsized non-keratinizing columnar to polyhedral cells arranged in anastomosing ribbon and large nest. It has complex in-folding of thick epithelial layers separated by fibrovascular septa. Tumor cells showed characteristic palisading arrangement of columnar cells, and perpendicularly distributed to the basement membrane. The cells had pale basophilic cytoplasm, oval nucleus and one or more nucleoli, and indistinct cellular border. Many tumor cell emboli were presented in lymphatics. Immunohistochemistry revealed that tumor cells were cytokeratin (CK) 19 and CK clone MNF116 positive and but CK7 and CK high molecular weight negative. Based on the gross, histopathologic, and immunohistochemical findings, the mass was diagnosed as transitional carcinoma in nasal cavity. In our best knowledge, this is the first report of transitional carcinoma originated from transitional zone of canine nasal cavity in Korea.
Concurrent occurrence of apocrine sweat gland adenocarcinoma and mammary gland complex adenoma in a dog
Park, Ji-Sung ; Jung, Ji-Youl ; Jo, Suk Hee ; Cheong, Jongtae ; Kang, Tae-Young ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 311~316
A 7-year-old female Shih Tzu dog with lots of masses in the whole mammary gland was presented to the surgery department of the Veterinary Teaching Hospital in the Cheju National University. After surgical excision, all mammary samples were referred to Pathology Department of Veterinary Medicine. Grossly, masses were measuring up to
and on cut surface of masses in right 1st, 3rd, 4th, 5th and left 1st, 3rd, 4th, 5th mammary masses were well delineated and firm, sulphur yellow, solid round to oval shape. Microscopically, most neoplastic sweat glands were severely proliferated in dermis and subcutis. Most tubules were lined by round to oval shaped epithelium with eosinophilic cytoplasm, hyperchromatic nuclei with high mitotic figures and severe central necrosis. The neoplastic epithelium also had PAS-positive diastase-resistant cytoplasmic granules, but negative with Perls iron stain. The left 2nd mass was well delineated, and had several dark brown areas and yellowish white glittered areas. Mass was well circumscribed with dense connective tissue. Neoplastic areas contained irregular sized mammary gland with papillary grown luminal epithelial cells in single or double cells layer with mitotic figures and small amounts of proliferated myoepithelial cells. Proliferated myoepithelial cells also produced slightly basophilic mucinous materials. Based on the gross, histopathologic and special staining characteristics, this dog was diagnosed as 90% of apocrine sweat gland aenocarcinoma and 10% mammary. complex adenomas in mammary masses. In our best knowledge, this is the first report for concurrent occurrence of apocrine sweat gland adenocarcinoma and mammary gland complex adenoma in mammary masses of the same dog.
Histopathologic Lesion of Mannheimia haemolytica pneumonia in calves
Jean, Young Hwa ; Roh, In Soon ; Lee, Kyung Hyun ; Lee, Kyung Woo ; Lee, Hee Soo ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 317~321
Four 5 month old calves were died after showing respiratory distress after long-distance transportation at winter season. They were diagnosed as fibrinous lobar pneumonia caused by Mannheimia (M.) haemolytica. Grossly, lungs were attached onto the pleura by fibrin, with a rich yellowish fluid in thorax. The cut surface of the lung was showed marbled pattern of the reddish or greyish consolidation and widened interlobular septa by fibrin deposition. Histopathologically, parenchymal necrosis was delineated by a band of the degenerated inflammatory cells, and distended interlobular septa with serofibrinous exudates and vascular thrombosis with alveolar capillaries degeneration and abundant serofibrinous exudates in alveoli. M. hemolytica were isolated from all calves, and bovine viral diarrhea virus and parainfluenza type 3 virus in one calf were detected by RT-PCR. Thus, it was concluded that this case was diagnosed as pneumonic mannheimiosis suggested by complex infection with viruses after long-distance transportation and coldness.
Granulosa cell tumor in a sow
Kim, Hyeong-Seok ; Kang, Sang-Chul ; Jung, Ji-YouI ; Kim, Hyun-Sup ; Kim, Dae-Yong ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 323~326
A two-year-old mixed breed sow was requested to the Veterinary Pathology Laboratory of Cheju National University with a clinical signs of severe abdominal pain and sudden death. Grossly, there was severe hemorrhage in abdominal cavity. Most of internal parenchymas and subcutaneous muscle showed severe pale discoloration. Both ovaries were enlarged with oval to round protruding multilobular masses and dark red in color. And they were firm and contained multiple small cysts in their cut surface. Histopathologically, numerous neoplastic granulosa cells had spherical-to-oval, hyperchromatic nuclei and scant eosinophilic cytoplasms were distributed with follicular pattern in ovarian masses. And the typical Call-Exner bodies, distinctive microcavityies, were observed in the center of small neoplastic follicles. Based on the gross and histopathologic findings, this case was diagnosed as granulosa cell tumor. In our best knowledge, this is believed to be the first report of granulosa cell tumor in a sow in Korea.
Effect of bitter melon (Momordica Charantia) on anti-diabetic activity in C57BLI/6J db/db mice
Jeong, Jae-Hwang ; Lee, Sang-Hwa ; Hue, Jin-Joo ; Lee, Ki-Nam ; Nam, Sang Yoon ; Yun, Young Won ; Jeong, Seong-woon ; Lee, Young Ho ; Lee, Beom Jun ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 327~336
Many herbal extracts have been reported to have a preventive or therapeutic effect of on diabetes mellitus. Momordica Charantia commonly known as bitter melon or karela has been reported to be a medicinal plant for treating various diseases including cancers and diabetes. The objectives of this study were to investigate anti-diabetic effects of bitter melon (BM) as determined by blood glucose levels, glucose tolerance test (GTT), insulin tolerance test (ITT), insulin and HbA1C activities in serum, serum biochemical and lipid levels, histopathology, immunohistochemistry and AMPK-
expression of skeletal muscle in male C57BL/6J db/db mice. There were four experimental groups including vehicle control, BM 10 mg/kg, BM 50 mg/kg, and BM 250 mg/kg. BM at doses of 10, 50, and 250 mg/kg was orally administered to the diabetic mice everyday for 8 weeks. The treatments of BM 10, 50, and 250 mg/kg significantly decreased the blood glucose level in the diabetic mice compared with vehicle control (p < 0.05). The treatments of BM 10 and 50 mg/kg significantly decreased the GTT, ITT and HbA1c levels in the diabetic mice compared with vehicle control (p < 0.05). All BM groups significantly decreased GOT, GPT, BUN, LDL and glucose levels in the diabetic mice compared with the vehicle control mice (p < 0.05). The livers of mice treated with the BM 10, 50, and 250 mg/kg showed a remarkable decrease in the number of lipid droplets compared with the vehicle control. The pancreas of mice treated with the BM 10, 50, and 250 mg/kg showed a remarkable increase in insulin concentration of
-cells compared with the vehicle control. In addition, the treatments of BM 10, 50, and 250 mg/kg actually increased the expression of AMPK-
compared with vehicle control. These results suggest that BM has a respectable anti-diabetic effect resulting from inhibition of blood glucose level and lipid level in serum and that consumption of BM may give a benefit for controlling diabetes mellitus in humans.
Phytic acid does not affect the formation of colonic aberrant crypt foci in Fe-overloaded male F344 rats
Lee, Yea Eun ; Hue, Jin-Joo ; Lee, Ki-Nam ; Nam, Sang Yoon ; Ahn, Byeongwoo ; Yun, Young Won ; Jeong, Jae-Hwang ; Lee, Beom Jun ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 337~345
There are accumulating evidences that high levels of dietary iron may play a role in colon carcinogenesis. Elevated iron status has been associated with oxidative stress. Phytic acid (PA) functions as an antioxidant by chelating divalent cations and prevents formation of reactive oxygen species responsible for cell injury and carcinogenesis. The protective effect of PA was investigated on formation of aberrant crypt foci (ACF) induced by azoxymethane (AOM) in iron-overloaded male F344 rats. After acclimation with AIN-93G purified diet (35 ppm Fe, normal control diet) for one week, animals were fed iron-overloaded diet (350 ppm Fe) and PA (0.5% or 2% PA in water) for 8 weeks. Animals received two (1st and 2nd week) injections of AOM (15 mg/kg b.w.) to induce colonic ACF. The colonic mucosa was examined for the total numbers of aberrant crypt (AC) and ACF after staining with methylene blue. The blood and serum were analyzed with a blood cell differential counter and an automatic serum analyzer. Iron-overloaded diet increased the concentration of iron in liver of the rats. But iron-related parameters in blood were not changed among experimental groups. The numbers of ACF per colon and AC per colon were
in the iron-overloaded F344 rats. The total AC was significantly increased, compared with normal-diet AOM control group (p < 0.05). The treatments of PA at the dose of 0.5% slightly decreased the number of ACF and AC per colon to
. However, there were no significant differences in the total numbers of ACF and AC between the AOM control group and PA (0.5% or 2%)-treated groups. These results suggest that PA may not affect the formation of ACF or AC induced by AOM in ironoverloaded F344 rats.
Toxicological effects of perfluorooctanoic acid in rats
Kim, Yong-Hoon ; Cho, Eun-Sang ; Kim, A-Young ; Kim, Sung-Hwan ; Park, Min-Sik ; Cho, Sung-Whan ; Ryu, Si-Yun ; Jung, Joo-Young ; Son, Hwa-Young ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 347~355
Perfluorooctanoic acid (PFOA), a member of the perfluoroalkyl acids that have wide commercial applications, is persistent organic pollutants widely spread throughout the environment and human population. But little is known about the adverse biological effects of the PFOA. In the present study, the toxicological effects of PFOA were investigated in rats. Sprague-Dawley rats (N = 10 in each group) were orally administered with PFOA in drinking water for 4 weeks (0, 100, 200, or 400 ppm in male, and 0, 200, 400, or 800 ppm in female). Three female rats given 800 ppm died during the study. PFOA treatment decreased the body weight gain and increased the liver weights in both genders. Serum biochemical investigations revealed significant increases in the aspartate aminotransferase, alanine transaminase, alkaline phosphatase, blood urea nitrogen, total cholesterol, and total bilirubin in male but in female. Serum estradiol (E2) levels were increased in all treated rats. Histopathologically, hepatocellular hypertrophy around central vein was noted in the liver of treated rats. No significant histopathological changes were noted in other organs. In conclusion, PFOA induced toxicological changes in the liver and increased serum E2 level which was not related to histopathological changes of endocrine and reproductive system.
Sample size and statistical power consideration for diagnostic test research
Kim, Eu Tteum ; Park, Choi Kyu ; Pak, Son Il ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 357~361
Although power analysis is of important tool of research, investigators in veterinary medicine are unaware of the concepts of the statistical power. Two types of error occur in classical hypothesis testing and, those errors should be avoided, if possible. Since power is highly dependent on the sample size, whenever declaring non-statistically significant result they should consider the potential for committing a Type II error in their studies, which refers to the probability of falsely stating that two treatments are equivalent despite true difference between them. Also, sample size determination is one of the most important tasks facing the researcher when planning a diagnostic study, and provides valuable information on the characteristics of a test performance. This type of analysis forms the basis for proper interpretation of test results. The aim of this article was to re-evaluate some selected studies on diagnostic test reported in the domestic veterinary publications to determine the power and necessary sample size for inequality testing to ensure the desired power. Power calculations were illustrated using real-life examples of comparison of a new test and a reference test for detecting antibodies of various animal diseases. Factors affecting to the power were also discussed.
A T-cell type multicentric Lymphoma affecting central nervous system in a Cocker Spaniel dog
Kim, Ju-Won ; Jung, Dong-In ; Kang, Byeong-Teck ; Yoo, Jong-Hyun ; Park, Chul ; Kim, Dae-Young ; Park, Hee-Myung ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 363~367
A 4-year-old female Cocker spaniel was presented with respiratory distress and abdominal distension. Pleural effusion, ascites, hepatosplenomegaly, and superficial lymphadenopathy were observed and multicentric lymphoma was diagnosed by cytological examination. Immunophenotyping of lymph node and bone marrow using polymerase chain reaction for antigen receptor rearrangement identified a stage V lymphoma originating from T-cell. Despite of systemic chemotherapy using L-asparagenase, vincristine, cyclophoaphamide and prednisolone, neurologic deficits came out and progressed. Cerebrospinal fluid analysis revealed neoplastic lymphocytic pleocytosis indicating central nervous system involvement of lymphoma. The postmortem diagnosis was confirmed based on the histology and imunohistochemistry.
Diagnostic imaging of nasal malignant melanoma in a dog
Jung, Joohyun ; Kwon, Jungkook ; Chang, Jinhwa ; Oh, Sunkyoung ; You, Mi-Hyeon ; Kim, Dae-Yong ; Yoon, Junghee ; Choi, Mincheol ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 369~374
A neutered male, 8-year-old, Pekingese, weighing 4.3 kg with a history of anorexia, sneezing, nasal discharge, and epistaxis for one month was referred. Soft tissue swelling around the nasal bone and small defects of the hard palate with a tiny round dark red mass were found on physical examination. The laboratory tests represented mild leukocytosis. On skull radiographs, soft tissue swelling and osteolytic change of the incisor bone, nasal bone, and maxilla were found. On computed tomography scan images, there was soft tissue attenuating opacity with calcified spots in the bilateral nasal cavities and frontal sinuses. Loss of nasal turbinate pattern and nasal septum was found. And destruction of the insicor bone, nasal bone, maxilla, hard palate, perpendicular palatine bone, and cribriform plate were identified. Nasal malignant melanoma was confirmed by nasal biopsy.
Evaluation for canine hip dysplasia in Golden and Labrador retrievers using PennHIP method in Korea
Choi, Jihye ; Kim, Hyunwook ; Kim, Hyejin ; Jang, Jaeyoung ; Kim, Mieun ; Yoon, Junghee ;
Korean Journal of Veterinary Research, volume 48, issue 3, 2008, Pages 375~379
Hips of the Golden and Labrador retrievers in Korea were evaluated with the University of Pennsylvania Hip Improvement Program (PennHIP) and the severity of joint laxity and degenerative joint disease (DJD) were compared to the PennHIP database. The distraction index (DI) of domestic Golden and Labrador retrievers was significantly higher than the DI of the PennHIP database. In the two breeds, the prevalence of DJD increased according to the DI. However, the severity of DJD did not show a positive correlation with the DI. Overweight dogs and dogs kept indoors showed more severe DJD and more prevalence of clinical signs. This report is thought to be the first case presentation of a large population of Golden and Labrador retrievers in Korea and the findings are representing the overall level of canine hip dysplasia (CHD) of domestic Retrievers, as the PennHIP method was not available in Korea until 2001. We can respect that the stock of retrievers can have a tighter hip joint through control of CHD using an accurate diagnostic method keyed to a phenotype especially concern for joint laxity using PennHIP method and an organized screening program. The clinical manifestation of dysplastic dog can be reduced through control of bodyweight and the environment with regular monitoring the hips with concern for joint laxity using PennHIP method.