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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Research
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Science
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Volume & Issues
Volume 49, Issue 4 - Dec 2009
Volume 49, Issue 3 - Sep 2009
Volume 49, Issue 2 - Jun 2009
Volume 49, Issue 1 - Mar 2009
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Anti-mycoplasmal and anti-inflammatory effect of Origanum vulgare extract against Mycoplasma hyopneumoniae
Choi, Myung-Jin ; Hwang, Mi-Hyun ; Choi, Jae-Young ; Lee, Joong-Su ; Park, Seung-Chun ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 185~193
In the present study, ten herbal extracts, Citrus unshiu Markovich, root and stem of Berberis koreana, Morus alba, Dendrobium moniliforme, Aster gramineus, A. scabar, Alisma canaliculatum, Fallopia japonica and Origanum (O.) vulgare were determined to examine anti-mycoplasmal activity. Among them, O. vulgare extract (OVE) showed strong anti-mycoplasmal activity and was analyzed by gaschromatography/ mass spectrometry (GC/MS). As the results, OVE was consisted of carvacrol (68.78%), o-cymene (9.80%), terpinene (7.61%) and thymol (4.03%) as main ingredients. To investigate inflammatory activity by intact pathogenic Mycoplasma hyopneumoniae (M. hyo) at 30
g/ml, we examined induced transcription of proinflammatory cytokines such as cyclooxygenase-2, tumor necrosis factor-a, interleukin (IL)-1, IL-6 and inducible nitric oxide synthase in RAW 264.7 cells. With the above results, we further investigated whether OVE could reduce inflammation induced by M. hyo at minimal inhibitory concentration. The result showed that 32
g/ml of OVE inhibited nitric oxide production by 60%. This study also evaluated the combination of OVE with antibacterials against M. hyo for application. Based on these results, it could be concluded that M. hyo induces inflammation in RAW 264.7 cells and OVE protects this inflammation, indicating that OVE may be useful for industrial animals.
Antibiotic resistance pattern of avian pathogenic Escherichia coli isolated from chickens
Kim, Myeong Suk ; Kwon, Hyuk Moo ; Sung, Haan Woo ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 195~200
Avian pathogenic Escherichia coli (APEC) is an important bacterial pathogen of chickens and causes colibacillosis such as airsacculitis, perihepatitis, omphalitis, peritonitis, salpingitis, and pericarditis. As the transfer of antibiotic resistance from animal to humans can be possible, surveillance on antibiotic resistance of APEC is very important. A total 34 APEC isolates from diseased chickens during the period from 2007 to 2009 were obtained. The susceptibility of the isolates to 13 antibiotics was determined by disc diffusion assay. Resistance to erythromycin was found in 97.1% of APEC isolated, followed by resistance to tetracycline (85.3%), doxycycline (82.3%), ampicillin (73.5%), sulfisoxazole (67.6%), enrofloxacin (67.6%), ciprofloxacin (64.7%), norfloxacin (61.7%) trimethoprim/sulfamethoxazole (52.9%), gentamycin (26.5%), amoxicillin (8.8%), colistin (5.9%), and amikacin (2.9%). The blaTEM genes were detected in 25 (100%) of the 25 ampicillin-resistant APEC isolates. Among the 29 tetracycline-resistant APEC isolates, tetA and tetB genes were detected in 18 (62.1%) and 9 (31%) isolates, respectively. Twenty six (76.5%) isolates were multiresistant to at least 6 antibiotics and seven (20.1%) isolates were multiresistant to at least 10 antibiotics. This results indicated that multiple antibiotic-resistant APEC is widespread in chicken flocks in Korea.
Evaluation of stability and potency of live attenuated rinderpest vaccine of lapinized-avianized tissue culture strain origin for the establishment of expiration period
Yeh, Jung-Yong ; Kim, Hyun-Ju ; Nah, Jin-Ju ; Park, Jee-Yong ; Lee, Ji-Hye ; Seo, Hyun-Ji ; Kweon, Chang-Hee ; Cho, In-Soo ; Moon, Jin-San ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 201~205
In this study, we evaluated the stability and potency of live attenuated rinderpest vaccines of lapinized-avianized tissue culture strain origin, which had been produced annually from 2005 to 2008. When immune responses to the vaccines were evaluated using two Holstein calves weighing 100~150 kg, neutralizing antibody titer of 1 : 16 was induced at 21 days post vaccination. When calves were also inoculated with vaccines lots that had been stored for 39 months at
, same level of antibody titer was observed. Using the virus titer test, we found that all batches of the vaccine that had been kept for 3, 10, 15, 22, 27, 34, 39, and 45 months showed no significant loss of titers, and fulfilled the requirement necessary (
) to be used as the national rinderpest vaccine reserve in Korea. In this study, we demonstrated that stability and potency of the rinderpest vaccines were maintained over three years when kept at
storage. This indicates that it maybe feasible to extend the expiration period of this vaccine from one year to three years.
Identification and molecular characterization of a rabbit hemorrhagic disease virus variant (KV0801) isolated in Korea
Yang, Dong-Kun ; Kim, Byoung-Han ; Lee, Kyung-Woo ; Kim, Ji-Yeon ; Kim, Hee-Jin ; Choi, Sung-Suk ; Chun, Ji-Eun ; Son, Seong-Wan ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 207~213
Rabbit hemorrhagic disease (RHD) is caused by RHD virus (RHDV) and is one of the most fatal diseases of rabbits. Acute death of rabbits occurred in a farm located in the Gyeonggi province of South Korea. The virus was isolated and confirmed as RHDV based on reverse transcription polymerase chain reaction and hemagglutination assay (HA), and the isolate was designated as KV0801. The nucleotide sequence of the complete VP60 gene of KV0801 was determined and the corresponding amino acid sequence was deduced. Molecular analysis showed that the KV0801 isolate can be classified as a pandemic antigenic variant strain, RHDVa. The VP60 nucleotide sequence and deduced amino acid homology between KV0801 and other Korean isolate, RHF89, which was isolated in 1988, were 92.1 and 94.3%, respectively. The pathogenicity of the KV0801 isolate at an HA titer ranging from 16,384 to 0.16 HA units was evaluated in five-month-old SFP rabbits. The rabbits inoculated with KV0801 isolate containing more than 1.63 HA units died within six days of inoculation. These results suggest that a highly pathogenic RHDVa is circulating in the rabbit populations of Korea.
Detection of potentially xenozoonotic viruses in the porcine ovary in Korea
Kang, Sang-Chul ; Jung, Ji-Youl ; Yang, Hyoung-Seok ; Park, Bong-Kyun ; Kim, Dae-Yong ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 215~220
The prevalence of potentially xenozoonotic viruses in the reproductive tract of female pigs in Korea was investigated by polymerase chain reaction (PCR). These viruses include porcine endogenous retrovirus (PERV), porcine reproductive and respiratory syndrome virus (PRRSV), swine hepatitis E virus (SHEV), porcine lymphotropic herpesvirus (PLHV), and porcine circovirus type 2 (PCV-2). Histopathological examination and PCR analysis were conducted using the ovaries of 70 slaughtered pigs that were collected from 14 farms in Jeju. Histopathologically, infiltrations of mononuclear inflammatory cells around the thick-walled coiled vessels in the ovarian medulla were observed in 15 cases. Based on the PCR method, PERV, PLHV, PRRSV, SHEV, and PCV-2 were detected in 69 (98.6%), 35 (50%), 5 (7.1%), 4 (5.7%), and 1 sample (1.4%), respectively. These results suggest that PERV and PLHV are the major xenozoonotic viruses in the porcine ovary. This study should aid in the development of a monitoring protocol for potential xenozoonotic agents and in the production of germ-free pigs for xenotransplantation.
Immunological relationships of FMD vaccine strain and Asia1 field isolate from East Asia
Park, Jong-Hyeon ; Ko, Young-Joon ; Kim, Su-Mi ; Lee, Hyang-Sim ; Lee, Kwang-Nyeong ; Cho, In-Soo ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 221~229
Foot-and-mouth disease (FMD) is the most contagious disease of mammals. The use of inactivated vaccine can be chosen to prevent or control FMD. However, vaccination against one serotype of FMDV doses not cross-protect against other serotypes and may not protect fully against some strains of the same serotype. Appropriate selection of vaccine strain is an important element in the control of FMD. The immunity of vaccine antigens should be matched against newly circulating viruses. The phylogenetic analysis of serotype Asia1 reported from China, Mongolia, North Korea and Russia since 2005 shows that they are all classified into genetic group V, but the strain, Asia1/Shamir (ISR/89) which have been used as a vaccine strain in Korea, is clustered into different genetic group. So, in this study the serological relationship between the isolate (Asia1/MOG/05; MOG) and the Shamir strain was determined by ELISA and virus neutralization test. Even though the matching value of the virus (MOG) against the vaccinated sera in target animals was not so high, the vaccinated animals elicited antibodies enough for protection after vaccinated once or twice. Conclusively, we suggest that the vaccine containing Asia1/Shamir antigen could protect the genetic group V strains circulating in East Asia currently if vaccinated twice or the more.
Relationship between biofilm formation and the antimicrobial resistance in the Staphylococcus spp. isolated from animal and air
Seo, Yeon-Soo ; Lee, Deog Young ; Kang, Mi Lan ; Lee, Won Jung ; Yoo, Han Sang ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 231~236
Biofilm has been described as a barrier, which produced by microorganisms to survive and protect themselves against various environments, like antibiotic agents. Staphylococcus spp. is a common cause of nosocomial and environmental infection. Thirty-six and thirty-five Staphylococci were isolated from animals and air, respectively. Based on the biofilm forming ability of the bacterium reported in our previous report, relationship between biofilm formation and antibiotic-resistance was investigated in this study. Regarding antibiotics susceptibility, cefazolin was the most effective agent to the bacteria. Strong biofilm-forming Staphylococcus spp. isolates might have a higher antibiotic resistance than weak biofilm isolates regardless of the presence of antibiotic resistance genes (p < 0.05). This result suggested that the chemical complexity of the biofilm might increase the antibiotic resistance due to the decrease of antibiotic diffusion into cells through the extensive matrix.
Existence of antibodies against bovine enterovirus in humans and various animals in Korea
Park, Jong-Hyeon ; Kim, Su-Mi ; Bang, Min-Woo ; Lee, Kwang-Nyeong ; Ko, Young-Joon ; Lee, Hyang-Shim ; Shim, Hang-Sub ; Cho, In-Soo ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 237~242
Bovine enteroviruses (BEVs) were separated into two groups, BEV-1 and BEV-2. BEVs, found in cattle worldwide, usually cause asymptomatic infections and are excreted in the feces of infected animals. Antibodies against BEV have been found in different species including human, cattle, sheep, goats, dogs, horses and monkeys in the world. This study aimed to investigate prevalence of the neutralizing antibodies for BEVs in human and animals in Korea. Antibodies against BEV-1 in humans, cattle, pigs, goats, horses and dogs were shown to be 46.8%, 48.3%, 70.6%, 11.5%, 11.5% and 6.3% respectively. Also, antibodies against BEV-2 were shown to be 98.7%, 68.1%, 89.2%, 59.4%, 9.4% and 96.9% respectively. We found that the neutralizing antibodies against these viruses are common in Korea. The prevalences of antibodies against BEV-1 were lower than those against BEV-2 in humans and in all animals except horses. These results showed that the BEV is considered endemic in cattle in many regions in Korea.
Genetic analysis of canine parvovirus vaccine strains in Korea
Yang, Dong-Kun ; Kim, Byoung-Han ; Kim, Yeon-Hee ; Lee, Kyung-Woo ; Choi, Sung-Suk ; Son, Seong-Wan ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 243~248
After the original identification of canine parvovirus (CPV) type 2 (CPV-2) in 1978, new antigenic variants such as CPV-2a, CPV-2b and CPV-2c have become widespread in the most countries. In this study, the genetic analysis of canine parvovirus was investigated in a total of 13 CPV vaccines, which have been licensed in Korea since late 1980s, and a field isolate of CPV from a dog with CPV infection clinical symptom. The partial VP2 gene of CPV was amplified and sequenced from 13 vaccine strains and one field isolate. The results showed that of the 13 vaccine strains, 10 strains belong to the CPV-2, 2 strains to CPV-2b, the remaining and one isolate to CPV-2a type, respectively. Several mutations of amino acids were detected at residues of the critical region of the commercial vaccine strains. These data suggest that new type of vaccines containing CPV-2a or CPV-2b/2c type may be required for the better prevention of new CPV infection in dog population in Korea, because CPV-2 contained in most licensed vaccines has been replaced by antigenic variants designated CPV-2a or CPV-2b/c in the worldwide dog population.
Primary hepatic hemangiosarcoma (HSA) in a Schnauzer dog
Kang, Min-Hee ; Heo, Ra-Young ; Park, Hee-Myung ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 249~252
A 6-year-old, neutered male, Schnauzer was presented with a one year history of weight loss and exercise intolerance. Physical examination revealed abdominal distention and ecchymosis on the abdominal skin. CBC and serum chemistry profiles revealed anemia and increased serum liver enzymes. Ultrasonography revealed a large liver mass which was characterized by multiple hypoechoic lesions. Postmortem examination revealed primary hepatic hemangiosarcoma. The tumor had extended to the mesentery and diaphragm, but distant metastasis was not found. This case report describes primary hepatic hemangiosarcoma which is very rare in a dog.
Acute and subacute toxicity of trichlorfon in guppies (Poecilia reticulata)
Heo, Gang-Joon ; Shin, Gee-Wook ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 253~256
The aim of the present study was to determine the acute and subacute potential of trichlorfon in guppies (Poecilia reticulatus). We first defined the 24 h median tolerance limit (
) of the fish to trichlorfon. Guppies were then treated with
trichlorfon concentrations to evaluate if any histological alterations occurred. The
value of trichlorfon was 11 ppm. This concentration resulted in acute toxicity to the gills and kidneys with edema, hyperplasia of the gill lamellae, and vacuolated degeneration and necrosis of renal tubular cells. In the case of subacute toxicity using a 10-fold dilution of the
value (1.1 ppm), no behavioral changes, external lesions or histopathological changes were observed. Therefore, safe concentration of trichlorfon might be 1.1 ppm in guppy for controlling parasitic infections.
Dehydrating and bacterial elimination effects of fecal dehydrating system for reducing bovine mastitis derived from environmental contamination
Kim, Dong Hyeok ; Lim, Jung Ju ; Lee, Jin Ju ; Kim, Dae Geun ; Chang, Hong Hee ; Lee, Seung Joo ; Lee, Yun Beom ; Chang, Dong Il ; Lee, Hu Jang ; Min, Won-Gi ; Kim, Sang Hun ; Oh, Kwon Young ; Kim, Suk ;
Korean Journal of Veterinary Research, volume 49, issue 3, 2009, Pages 257~263
Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. Escherichia coli and Staphylococcus aureus are the major causative agents of bovine mastitis. These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Bovine mastitis causative micro-organisms can survive in 1-2 weeks in feces and bed complexes. Low level of percentage of water content (PWC) of feces and bed complexes can reduce the spreading of bovine mastitis incidence from environmental contamination. In this study, we developed the fecal dehydrating system and determined the elimination rates of bovine mastitis causative agent from feces and bed complexes. To develop the fecal dehydrating system, the screw pressurized dehydrating system was used and the maximum rate of dehydrating was reached to 52% PWC using 90% PWC (wet base) of fecal and bed complexes. The elimination rates of the dehydrating system for E. coli and S. aureus were reached at 41.19
7.84% to 62.55
8.71% in various percentages of PWC of feces and bed complexes (80, 85 and 90%). These results suggested that the application of fecal dehydrating system would reduce the exposure of dairy cattle to bovine mastitis causing agents contaminated feces and bed complexes, and can be used for environmental bovine mastitis control avoiding misuse or abuse of chemical disinfectants and antibiotics in dairy farm.