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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Research
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Science
Editor in Chief :
Volume & Issues
Volume 52, Issue 4 - Dec 2012
Volume 52, Issue 3 - Sep 2012
Volume 52, Issue 2 - Jun 2012
Volume 52, Issue 1 - Mar 2012
Selecting the target year
Effects of vitamin C on oxidative status in pigs anesthetized with isoflurane
Lee, Jae Yeon ; Kim, Myung Cheol ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 153~155
This study investigated the effects of vitamin C on oxidative stress induced by volatile anesthetics in pigs. One group of pigs was used as an anesthesia control group (group 1), and they were anesthetized with isoflurane in oxygen and saline (0.9% NaCl) was injected intravenously. The other group (group 2) was anesthetized with isoflurane and injected intravenously with vitamin C. Total oxidant status, total antioxidant status, and the oxidative stress index in group 2 were significantly different compared with those in group 1. The results showed that intravenous administration of vitamin C decreased oxidative stress during isoflurane anesthesia in pigs.
Safety and outcomes of subconjunctival allogenic mesenchymal stem cell transplantation in canine experimental corneal defects
Kim, Ju-Won ; Lee, So-Young ; Park, Hee-Myung ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 157~162
Corneal injury is very common clinical condition in veterinary medicine and delayed or incomplete corneal healing has the potential of vision loss due to the loss of corneal transparency. For the reconstruction of corneal epithelium, tissue graft and cell transplantation have been prosperously investigated. The purpose of this study was to evaluate the clinical value and short-term safety of application of cultured allogenic mesenchymal stem cells (MSCs) in the treatment of canine experimental corneal defect. Corneal defects were surgically generated in the central corneas of healthy beagle dogs and cultured canine allogenic MSCs were transplanted via subconjunctival injection. Although mean healing time, the rate of epithelial regeneration, and the degree of corneal transparency were not significantly improved after MSC transplantation, significant immune reaction or incompatibility reaction was not detected except transient local irritation. These results propose the possibility of MSC application as a new regenerative medicine in canine ocular disorders.
Effects of 5-HT
selective receptor agonist, mosapride citrate on electrocardiogram in dogs
Chae, Ji Sang ; Ahn, Jin Ok ; Coh, Ye Rin ; Park, Chong Woo ; Youn, Hwa Young ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 163~167
Mosapride stimulated dietary motility was introduced because of the arrhythmogenic effect of cisapride. Cisapride, 5-HT receptor agonist, induces prolongation of QT interval. Additionally, this condition can raise the possibility of acute, "malignant" arrhythmias such as torsade de pointes. It is hard to find any reports about effects of mosapride on cardiac parameters in dogs. By confirming electrocardiogram (ECG) parameters, the surface extremity leads ECG that was obtained from the four-limb electrodes and which was recorded by an ECG recorder after administration of mosapride 3 mg/kg PO b.i.d, and mosapride 3 mg/kg with itraconazole 5 mg/kg PO b.i.d, respectively. QT interval was shortened on the days of 3, 5, and post-day 1 in both mosapride 3 mg/kg administrated group and mosapride with itraconazole group. Heart rate increased significantly. QTc was slightly prolonged in mosapride administration group and mosapride with itraconazole group. However, all dogs of QTc were in normal variation (150~250 msec). Besides, the dogs showed no side effects reported in human medicine during the administration with these drugs. Although mosapride can increase the heart rate, this study suggest that mosapride may be useful for the dogs with disorders of gastrointestinal motility because of no fatal arrhythmogenic effect inspite of administration with itraconazole in dogs.
Isolation, characterization, and evaluation of Bacillus thuringiensis isolated from cow milk
Kweon, Chang-Hee ; Choi, Sang-Yoon ; Kwon, Hyog-Young ; Kim, Eun-Hye ; Kang, Hyun-Mi ; Moon, Jin-San ; Jang, Geum-Chag ; Lee, Hee-Soo ; Kang, Seung-Won ; Kim, Jong-Man ; Pyo, Suhkneung ; Rhee, Dong-Kwon ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 169~176
Probiotics colonize the intestines and exert an antibacterial effect on pathogens. Therefore, probiotics could be used as a preventive agent against lethal infections. To isolate probiotic microorganisms, 116 bacterial strains were isolated from healthy cow's milk and were subjected to Gram-stain, morphology and biochemical analyses, Vitek analysis, and 16S rRNA analysis. One of the strains identified as Bacillus (B.) thuringiensis 87 was found to grow very well at pH 4.0~7.0 and to be resistant to high concentrations of bile salts (0.3~0.9% w/v). B. thuringiensis was susceptible to the antibiotics used in the treatment of bovine mastitis, yet it exhibited an antimicrobial effect against Staphylococcus (S.) aureus 305. Moreover, it protected mice from experimental lethal infections of E. coli O55, Salmonella typhimurium 01D, and S. aureus 305 through a significant induction of interferon-
, even at four-week post-administration of B. thuringiensis. Although oral administration of B. thuringiensis 87 did not provide significant protection against these lethal challenges, these results suggest that B. thuringiensis 87 could be a feasible candidate as a probiotic strain.
Serotype and antimicrobial susceptibility of Actinobacillus pleuropneumoniae isolates from pigs in Korea
Jung, Ji-Youl ; Jang, Hyun ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 177~181
Actinobacillus (A.) pleuropneumoniae is the causative agent of pleuropneumonia which is one of the most important respiratory diseases in pigs worldwide. A total of 32 A. pleuropneumoniae isolates from diseased pigs during 2008 to 2010 were serotyped by polymerase chain reaction method. The susceptibility of the isolates to 13 antimicrobial agents were determined by disk diffusion test. In all the 32 isolates examined in this study, serotype 5 (16 isolates: 50%), 1 (7 isolates: 21.9%), 2 (5 isolates: 15.6%) and 12 (1 isolate: 3.1%) were found. Of all tested antimicrobial agents, resistance to oxytetracycline was found in 96.9% of isolates, followed by resistance to amikacin (81.2%), neomycin (68.7%), kanamycin (53.1%), penicillin (50.0%), gentamicin (43.7%), florfenicol (25.0%), ampicillin (18.7%), colistin (9.4%), trimethoprim/sulfamethoxazole, ceftiofur (8.3%), amoxicillin/clavulanic acid (3.1%) and enrofloxacin (0%). Oxytetracycline or florfenicol-resistant isolates were examined for the presence of resistance gene. Among the 31 oxytetracycline-resistant isolates, tetB, tetH and tetO genes were detected in 22 (71%), 8 (26%) and 1 (3%) isolates, respectively. The floR genes were detected in 8 (100%) of the 8 florfenicol-resistant A. pleuropneumoniae isolates.
The expression of Foxp3 protein by retroviral vector-mediated gene transfer of Foxp3 in C57BL/6 mice
Hwang, Insun ; Ha, Danbee ; Bing, So Jin ; Jeon, Kyong-Leek ; Ahn, Ginnae ; Kim, Dae Seung ; Cho, Jinhee ; Lim, Jaehak ; Im, Sin-Hyeog ; Hwang, Kyu-Kye ; Jee, Youngheun ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 183~191
The maintenance of peripheral immune tolerance and prevention of chronic inflammation and autoimmune disease require
T cells (regulatory T cells). The transcription factor Foxp3 is essential for the development of functional, regulatory T cells, which plays a prominent role in self-tolerance. Retroviral vectors can confer high level of gene transfer and transgene expression in a variety of cell types. Here we observed that following retroviral vector-mediated gene transfer of Foxp3, transductional Foxp3 expression was increased in the liver, lung, brain, heart, muscle, spinal cord, kidney and spleen. One day after vector administration, high levels of transgene and gene expression were observed in liver and lung. At 2 days after injection, transductional Foxp3 expression level was increased in brain, heart, muscle and spinal cord, but kidney and spleen exhibited a consistent low level. This finding was inconsistent with the increase in both
T cell and
T cell frequencies observed in peripheral immune cells by fluorescence-activated cell-sorting (FACS) analysis. Retroviral vector-mediated gene transfer of Foxp3 did not lead to increased numbers of
T cell and
T cell. These results demonstrate the level and duration of transductional Foxp3 gene expression in various tissues. A better understanding of Foxp3 regulation can be useful in dissecting the cause of regulatory T cells dysfunction in several autoimmune diseases and raise the possibility of enhancing suppressive functions of regulatory T cells for therapeutic purposes.
Clinico-pathological Features of Chlamydophila psittaci Infection in Parrots and Genetic Characterization of the Isolates
Kim, Il-Hwan ; Jang, Jin-Wook ; Lee, Su-Hyung ; Kim, Dae-Yong ; Seong, Won-Jin ; Kwon, Hyuk-Joon ; Kim, Jae-Hong ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 193~198
Avian chlamydiosis is caused by Chlamydophila psittaci and considered as one of an important zoonotic disease throughout the world. Among more than 400 avian species including poultry and pet birds susceptible to the disease, psittacine birds were known to be mostly susceptible hosts. In Korea, no outbreak of the disease and genetic analysis of the agent in poultry and pet birds have been reported. With histopathological findings and genetic identification of a causative agent, avian chlamydiosis was identified in parrots submitted from the same pet bird farm in 2006 and 2009 for the diagnosis. Based on genetic sequences and phylogenetic analysis of ompA gene, the two isolates of Chlamydophila psittaci showed 100% of genetic similarity and belonged to genotype A, suggesting that the same agent might be continuously circulated in the farm. This result indicates that serological survey of the disease in pet bird farms and impact of the disease on significance in public health may be further studied.
An immunohistochemical study on the initial development of 155 kd neurofascin in rat sciatic nerve
Won, Hee-Young ; Cho, Ik-Hyun ; Lee, Jong-Hwan ; Nahm, Sang-Soep ; Choe, Nong-Hoon ; Park, Jae-Hyung ; Chang, Byung-Joon ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 199~203
Neurofascin, one of the members of L1CAM, has been known to have some important roles during the development of nerve fibers. In order to investigate the role of neurofascin associated with the development of nerve fibers in the rat sciatic nerve, the initial development of NF155 in the paranode was studied with immuno-fluorescence and immuno-electron microscopy. The result of the present study showed NF155 was not detected in the fetal sciatic nerve and began to reveal at the postnatal day 0 (P0) and dramatically increased by time lapse until postnatal day 7 (P7). NF155 was prominently localized in the axolemma of paranode and not detected in the central region of node of Ranvier. According to the present study, NF155 is likely to have some relationships with the formation of paranode and myelin sheath.
Diagnostic imaging of malignant insulinoma in a dog
Choi, Jihye ; Keh, Seoyeon ; Kim, Sungsoo ; Lee, Su-Hyung ; Kim, Hyejin ; Choi, Heeyeon ; Lim, Younji ; Kim, Hyunwook ; Kim, Ahyoung ; Kim, Dae-Yong ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 205~208
Endocrine test data from a 13-year old intact female Maltese was indicative of the presence of an insulinoma, however ultrasonography identified a pancreatic mass only after 10 months after the first admission. Following identification of both pancreatic tumor and hepatic metastasis on computed tomography (CT), surgical excision of the mass was attempted. However, total excision failed because of tumor adhesion to adjacent large vessels. The pancreatic mass was monitored over the next 25 months via ultrasonography, CT, and positron emission tomography-computed tomography (PET-CT). Histopathological and immunohistochemical data confirmed the diagnosis of insulinoma with hepatic metastasis.
Application of mycophenolate mofetil for immune-mediated hemolytic anemia in two dogs
Park, Hyung-Jin ; Lee, Dae-Yong ; Song, Kun-Ho ;
Korean Journal of Veterinary Research, volume 52, issue 3, 2012, Pages 209~211
A 5-year-old castrated male Shih-tzu dog and a 9-year-old intact female Schnauzer dog were presented with anorexia and depression. These 2 dogs were diagnosed based on which criteria as primary immune-mediated hemolytic anemia (IMHA). Blood examination showed anemia, spherocytosis, auto-agglutination and total bilirubinemia in 2 cases. These dogs were recovered by treatment of mycophenolate mofetil and prednisolone, and showed good prognosis until now. Mycophenolate mofetil is recommended as initial treatment in canine IMHA.