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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Research
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Science
Editor in Chief :
Volume & Issues
Volume 53, Issue 4 - Dec 2013
Volume 53, Issue 3 - Sep 2013
Volume 53, Issue 2 - Jun 2013
Volume 53, Issue 1 - Mar 2013
Selecting the target year
Pathological description and immunohistochemical demonstration of ovine abortion associated with Toxoplasma gondii in Iran
Rassouli, Maryam ; Razmi, Golam Reza ; Movassaghi, Ahmad Reza ; Bassami, Mohammad Reza ; Sami, Mehrdad ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 1~5
DOI : 10.14405/kjvr.2013.53.1.001
The obligatory intracellular protozoan parasite Toxoplasma gondii is a major world wide cause of infectious ovine abortion. In some different diagnostic techniques that are being used to detect this pathogen in ovine fetuses, immunohistochemistry (IHC) is a very sensitive and expensive one. Histopathology is not truly a specific and sensitive test for Toxoplasma infection but it can be helpful to choose some suspected tissues for IHC. In this study 9.5% of 200 samples (aborted ovine fetuses internal organs such as brain, liver, heart, lung, kidney, spleen) (4.6~14.4% with 95% CI) were positive in IHC with a very good logical agreement among different diagnostic techniques (
, 0.8) and with no significant difference among different fetal age groups (p > 0.05).
Application of varimax rotated principal component analysis in quantifying some zoometrical traits of a relict cow
Pares-Casanova, P.M. ; Sinfreu, I. ; Villalba, D. ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 7~10
DOI : 10.14405/kjvr.2013.53.1.007
A study was conducted to determine the interdependence among the conformation traits of 28 "Pallaresa" cows using principal component analysis. Originally 21 body linear measurements were obtained, from which eight traits are subsequently eliminated. From the principal components analysis, with raw varimax rotation of the transformation matrix, two principal components were extracted, which accounted for 65.8% of the total variance. The first principal component alone explained 51.6% of the variation, and tended to describe general size, while the second principal component had its loadings for back-sternal diameter. The two extracted principal components, which are traits related to dorsal heights and back-sternal diameter, could be considered in selection programs.
Sequential hepatic ultrastructural changes and apoptosis in rabbits experimentally infected with Korean strain of rabbit hemorrhagic disease virus (RHDVa)
Park, Jung-Won ; Chun, Ji-Eun ; Bak, Eun-Jung ; Kim, Han ; Lee, Myeong-Heon ; Hwang, Eui-Kyung ; Kim, Jae-Hoon ; Lee, Chung-Bok ; Woo, Gye-Hyeong ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 11~17
DOI : 10.14405/kjvr.2013.53.1.011
In this study, to understand the pathogenesis of new rabbit hemorrhagic disease virus (RHDVa) serotype, we carried out to administrate RHDVa to rabbits, and to examine sequential electron microscopic changes and relationship between pathogenesis and apoptosis. TUNEL-positive cells began to be observed from 24 hours after inoculation (HAI) and the number of positive cells was slightly increased with the course of time. Whereas marked increase of positive cells was seen in the liver from the rabbits died acutely. Typical viral particles with cup-like projections and a diameter of 30~40 nm were detected in homogenized liver samples and tissues at 36 and 48, and 48 HAI, respectively. Ultrastructurally, glycogen deposition was observed from the first stage of hepatocellular degeneration by RHDVa infection and then, swelling and disruption of cristae of mitochondria by viral particles, swelling of smooth endoplasmic reticulum, vacuoles and vesicles were detected. Condensation, margination and fragmentation of chromatin were observed in degenerative hepatocytes at 36 and 48 HAI, indicating apoptotic bodies. These data offer that hepatocytic apoptosis by RHDV infection could be closely related with mitochondrial impairment in the hepatocytes.
Classification of acute clinical mastitis on the base of vital signs and complete blood count test in dairy cows
Hur, Tai-Young ; Kang, Seog-Jin ; Jung, Young-Hun ; Lee, Hyun-June ; Ki, Kwang-Seok ; Choe, Chang-Yong ; Suh, Guk-Hyun ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 19~23
DOI : 10.14405/kjvr.2013.53.1.019
This study was carried out to evaluate the diagnosis of acute clinical mastitis (ACM) which was based on the vital signs and complete blood count (CBC) tests in dairy cows. Twenty eight dairy cows diagnosed with ACM, were selected for the study between Jan 2003 and July 2006 in the National Institute of Animal Science. Based on their vital signs (rectal temperature, depression, rumen contraction and, dehydration status), ACM was divided into three different classes; mild, moderate and severe forms. In addition, ACM cows were subjected to CBC tests for further diagnosis of ACM. Of the 27 dairy cows diagnosed with ACM, 3 cows were determined to have a mild form, while moderate and sever forms were each observed in twelve cows. Among of them, 4 cows died, 5 cows were culled and 18 cows were recovered. In the mild form, all haematological parameters were comparable with normal values. However, leukopenia, due to neutropenia and lymphocytopenia, appeared characteristically in the moderate and severe forms. Using the observation of vital signs in conjunction with CBC tests, the diagnosis of ACM is more accurate, and is helpful in making decisions of whether treatment or culling of dairy cows infected with ACM is most appropriate.
Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp.
Shin, Seung Won ; Cha, Seung Bin ; Lee, Won-Jung ; Shin, Min-Kyoung ; Jung, Myunghwan ; Yoo, Anna ; Jung, Byeng Yeal ; Yoo, Han Sang ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 25~28
DOI : 10.14405/kjvr.2013.53.1.025
The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle (
) of Salmonella spp. was
while non-Salmonella spp. was
. Correlation coefficients of standard curves constructed using
versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity (
; slope = 3.563). Minimum level of detection with the method was >
colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.
Changes of characterization of Salmonella Typhimurium isolate following sequential exposures to porcine neutrophil
Lee, Hee-Soo ; Kim, Aeran ; Youn, Min ; Lee, Ji-Youn ; Lim, Suk-Kyung ; Kang, Ho-Young ; Yoo, Han Sang ; Park, Jung-Won ; Wee, Sung-Hwan ; Jung, Suk-Chan ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 29~35
DOI : 10.14405/kjvr.2013.53.1.029
To develop a live vaccine candidate using an attenuated strain of Salmonella Typhimurium (ST), biochemical properties, plasmid profile, PFGE patterns and pathogenic analysis of the ST isolate were carried out after sequential passage of the ST isolate in porcine neutrophils. By the passage, the ability of the neutrophil-adapted isolate to utilize d-xylose was lost, while the ability of the strain to ferment trehalose was delayed after 2 or more days of the culture. Also, changes including deletion of the gene fragments were observed in PFGE analysis of the neutrophil-adapted isolates. Two plasmids, 105kb and 50kb, were cured in the strain passaged over 15 times in porcine neutrophils. The 50% of lethal dose (
) of the parent strain was changed from
by the passage in intraperitoneal injection of the strains into mice. These results suggested that bacterial genotypic and phenotypic responses might be globally altered depending on the inside environment of neutrophils.
Characterization of multipotent mesenchymal stem cells isolated from adipose tissue and bone marrow in pigs
Lee, Ah-Young ; Choe, Gyeong-Im ; Nah, Jin-Ju ; So, ByungJae ; Lee, Kyung-Woo ; Chang, Ki-Yoon ; Song, Jae-Young ; Cha, Sang-Ho ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 37~42
DOI : 10.14405/kjvr.2013.53.1.037
Mesenchymal stem cells (MSCs) have ability to differentiate into multi-lineage cells, which confer a great promise for regenerative medicine to the cells. The aim of this study was to establish a method for isolation and characterization of adipose tissue-derived MSC (pAD-MSC) and bone marrow-derived MSC (pBM-MSC) in pigs. Isolated cells from all tissues were positive for CD29, CD44, CD90 and CD105, but negative for hematopoietic stem cell associated markers, CD45. In addition, the cells expressed the transcription factors, such as Oct4, Sox2, and Nanog by RT-PCR. pAD-MSC and pBM-MSC at early passage successfully differentiated into chondrocytes, osteocytes and adipocytes. Collectively, pig AD-MSC and BM-MSC with multipotency were optimized in our study.
Efficacy and clinical trials of Salenvac-T, bivalent killed vaccine containing Salmonella Enteritidis and Salmonella Typhimurium
Cho, Youngjae ; Kang, Zheng-Wu ; Kang, Kyung-Soo ; Jeong, Seunghwan ; Yoon, Hee-Jun ; Suh, Seungwon ; Hahn, Tae-Wook ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 43~48
DOI : 10.14405/kjvr.2013.53.1.043
Commercial bivalent killed Salmonella vaccine Salenvac-T has been used in several countries in order to prevent salmonellosis with Salmonella enterica serovars Enteritidis (SE) and Typhimurium (ST) in poultry. However, this vaccine has not been used in poultry farms in South Korea. In this study, we evaluated the efficacy of Salenvac-T vaccine to protect against the challenge of virulent SE and ST, and the effect of the vaccine on egg production and mortality in layer hens. The colonization of liver, spleen and cecum with challenged SE and ST was reduced in vaccinated chickens compared with that of unvaccinated control group. The twice vaccination with Salenvac-T induced elevated antibody responses against both SE and ST detected by enzyme-linked immunosorbent assay (ELISA). The higher average hen-day production was observed in the vaccinated layer hens than in the unvaccinated layer hens without significance. The average mortality was lower in the vaccinated layer hens during the experiment period. The antibody responses to both SE and ST were persistently detected in the vaccinated layers. In summary, vaccination with Salenvac-T reduces colonization of internal organs and induces good antibody responses, thereby results in higher performance and lower egg contamination with SE and ST in layer hens.
Temporospatial clustering analysis of foot-and-mouth disease transmission in South Korea, 2010~2011
Bae, Sun-Hak ; Shin, Yeun-Kyung ; Kim, Byunghan ; Pak, Son-Il ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 49~54
DOI : 10.14405/kjvr.2013.53.1.049
To investigate the transmission pattern of geographical area and temporal trends of the 2010~2011 foot-and-mouth disease (FMD) outbreaks in Korea, and to explore temporal intervals at which spatial clustering of FMD cases space-time analysis based on georeferenced database of 3,575 burial sites, from 30 November 2010 to 23 February 2011, was performed. The cases represent approximately 98.1% of all infected farms (n = 3,644) during the same period. Descriptive maps of spatial patterns of the outbreaks were generated by ArcGIS. Spatial Scan Statistics, using SaTScan software, was applied to investigate geographical clusters of FMD cases across the country. Overall, spatial heterogeneity was identified, and the transmission pattern was different by province. Cattle have more clusters in number but smaller in size, as compared to the swine population. In addition, spatiotemporal analysis and the comparison of clustering patterns between the first 7 days and days 8 to 14 of the outbreak revealed that the strongest spatial clustering was identified at the 7-day interval, although clustering over longer intervals (8~14 days) was also observed. We further discussed the importance of time period elapsed between FMD-suspected notice and the date of confirmation, and emphasized the necessity of region-specific and species-specific control measures.
A morphological study of vomeronasal organ of Korean black goat (Capra aegagrus hircus)
Park, Changnam ; Yang, Wonjun ; Bae, Yeonji ; Lee, Yongduk ; Kang, Wanchoul ; Ahn, Meejung ; Shin, Taekyun ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 55~60
DOI : 10.14405/kjvr.2013.53.1.055
The vomeronasal organ (VNO) plays an important role in reproduction and social activities in ruminants including goats. A morphological study on the structure of VNO and its epithelial cells was carried out in Korean black goats. Grossly, the VNO of Korean goats opens into mouth through incisive ducts. Microscopically, the epithelium of VNO consisted of medial sensory epithelium and lateral non-sensory epithelium. Several blood vessels and nerve bundles were observed in the lamina propria encased by vomeronasal cartilage. Immunohistochemical staining showed that protein gene product (PGP) 9.5 was immunostained in the receptor cells of the sensory epithelium and in some cells of the non-sensory epithelium. Galectin-3 was mainly observed in the supporting cells of sensory and non-sensory epithelium. Lectins including wheat germ agglutinin, Ulex europaeus agglutinin, Bandeiraea simplicifolia lectin Isolectin B4, Dolichos biflorus agglutinin and soybean agglutinin used in this study were bound in VNO sensory, non-sensory epithelia as well as in the lamina propria with varying intensity. Collectively, this is a first descriptive morphological study of VNO of Korean black goat with special reference to lectin histochemistry.
Hepatic extraskeletal osteosarcoma with systemic metastasis in a dog
Johnson, Crystal ; Kim, Yongbaek ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 61~64
DOI : 10.14405/kjvr.2013.53.1.061
A ten-year-old dog was presented with pancreatitis and increased hepatic enzymes. On necropsy a large firm mass was observed in the liver extending to the gall bladder. Smaller masses with similar texture were found in multiple organs including lung, stomach, pancreas, lymph nodes, omentum, and mesentery. Neoplastic cells were spindle shaped with prominent osteoid production and occasional trabeculae of woven bone. Tumor cell emboli were observed in the blood vessels and lymphatics of the omentum and stomach. Hepatic osteosarcoma with systemic metastasis is very rare and may serve to broaden the diagnostic spectrum of hepatic and pancreatic diseases in dogs.
Seroprevalence of hepatitis E virus in zoo animal species in Korea
Song, Young-Jo ; Kim, Bo-Sook ; Park, Woo-Jung ; Park, Byung-Joo ; Lee, Seul-Kee ; Shin, Jong-Il ; Lee, Nak-Hyung ; Lee, Joong-Bok ; Park, Seung-Yong ; Song, Chang-Seon ; Seo, Kun-Ho ; Choi, In-Soo ;
Korean Journal of Veterinary Research, volume 53, issue 1, 2013, Pages 65~68
DOI : 10.14405/kjvr.2013.53.1.065
Hepatitis E virus (HEV) can infect not only human but also several animals. This study has been conducted to evaluate the comprehensive anti-HEV seroprevalence in zoo animals in Korea. Anti-HEV antibodies were identified in 14 of 64 zoo animal species. HEV antibodies were detected for the first time in Eurasian Lynx, Setland Pony, Fallow Deer, Ezo Sika, Formosa Deer, East Wapitis, Barasingha, Corriedale, American Bison, Guanacos, Reticulated Giraffe, and Saanen. These results indicate that the several zoo animal species were exposed to HEV.