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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
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Korean Journal of Veterinary Research
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Science
Editor in Chief :
Volume & Issues
Volume 53, Issue 4 - Dec 2013
Volume 53, Issue 3 - Sep 2013
Volume 53, Issue 2 - Jun 2013
Volume 53, Issue 1 - Mar 2013
Selecting the target year
Surveillance of wild birds for avian influenza virus in Korea
Lee, Dong-Hun ; Song, Chang-Seon ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 193~197
DOI : 10.14405/kjvr.2013.53.4.193
Avian influenza viruses (AIV) have been isolated from a wide range of domestic and wild birds. Wild birds, predominantly ducks, geese and gulls form the reservoir of AIV in nature. The viruses in wild bird populations are a potential source of widespread infections in poultry. Active surveillance for AIV infection provides information regarding AIV distribution, and global AIV surveillance can play a key role in the early recognition of highly pathogenic avian influenza (HPAI). Since 2003 in Korea, there have been four H5N1 HPAI outbreaks caused by clade 2.5, 2.2 and 2.3.2. Therefore, improvement of AIV surveillance strategy is required to detect HPAI viruses effectively. This article deals with the major events establishing the role of wild birds in the natural history of influenza in Korea. We highlighted the need for continuous surveillance in wild birds and characterization of these viruses to understand AIV epidemiology and host ecology in Korea.
Evaluation of systemic and mucosal immune responses in mice administered with novel recombinant Salmonella vaccines for avian pathogenic Esherichia coli
Oh, In-Gyeong ; Lee, John Hwa ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 199~205
DOI : 10.14405/kjvr.2013.53.4.199
Avian pathogenic Escherichia coli (APEC) is a causative agent for a number of extra intestinal diseases and account for significant losses to the poultry industry. Since protective immunity against APEC is largely directed to virulence antigens, we have individually expressed four different viulence antigens, papA, papG, IutA, and CS31A, using an attenuated Salmonella Typhimurium and a plasmid pBB244. Following oral immunization of mice with combination of two or four of these strains, serum IgG and mucosal IgA responses were elicited against each antigen represented in the mixture. The antigen-specific mucosal IgA responses were significantly higher in the group of mice immunized with the heat-labile Escherichia coli enterotoxin B subunit (LTB) strain than those in the group of mice immunized without the LTB strain. While, there was no significant difference between these two groups in antigen-specific serum IgG responses. The results showed that LTB could act as mucosal immune adjuvant. To assess the nature of immunity, the distribution of antigen-specific IgG isotypes was analyzed. All groups promoted Th1-type immunity as determined by the IgG2a/IgG1 ratio. Thus, our findings provided evidence that immunization with a combination of several vaccine strains is one of the strategies of developing effective vaccines against APEC.
Conservative neural symmetry of the caprine mandible
Pares-Casanova, Pere M. ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 207~210
DOI : 10.14405/kjvr.2013.53.4.207
Fifteen dry dentate and complete mandible samples from the White Rasquera goat breed were studied for symmetry. Thirty-one landmarks were digitally located on the images of the lateral and medial aspects of each hemimandible. Distances between these landmarks allowed the evaluation of the whole hemimandible and also the neural mandible. In the studied samples, the mandible was rather symmetrical, especially in the medial neural part, and in general, there was no side dominance. Only the diastema differed significantly between the sides, and this was related to the rostral part (incisive arch). The incisive region was the least symmetrical region of the caprine mandible, indicating a modular structure more conservative for the neural part. If unsigned asymmetry is interpreted as a measure of developmental stability, then the studied breed presented a marked ability to develop in good fitness despite the harsh environment. The measurements presented here can also be used as a reference for researchers designing experimental studies, especially on mandibular catch-up growth, and as an aid for zooarchaeologists comparing results from dead animals with those from living goat populations.
Development and evaluation of protective capacity of Salmonella Enteritidis polyphosphate kinase-deleted and temperature-sensitive mutant
Kim, Kiju ; Park, Soyeon ; Cho, Youngjae ; Kwak, Jeong-Yeon ; Kang, Zheng-Wu ; Kim, Eun-Hee ; Choi, Hwan-Won ; Won, Ho-Keun ; Noh, Yun-Hee ; Hahn, Tae-Wook ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 211~216
DOI : 10.14405/kjvr.2013.53.4.211
This study was focusing on evaluating the protection of polyphosphate kinase (ppk) deleted and/or temperature-sensitive (ts) Salmonella Enteritidis (SE) as an attenuated vaccine in chickens. We constructed SEppk, SEts and SEppk::ts mutants and screened those mutants by growth capability in vitro, protection study in mice model and antibody response in chickens. Among the mutants, SEppk::ts-3 was selected because it showed higher growth capability, good protection against highly virulent SE in mice model, and good antibody response in chickens. SEppk::ts-3 also showed good protection against highly virulent SE isolate because it decreased colonization of virulent SE challenge strain in spleen, liver and cecum compared with the non-vaccinated control. The SEppk::ts-3 mutant showed cross-protection against S. Gallinarum (SG) challenge although the its cross-protection rate was a little lower than that of SG9R, a commercial vaccine against SG infection. To use for live attenuated vaccine in chickens, it should further be characterized.
Expression of the S glycoprotein of transmissible gastroenteritis virus (TGEV) in transgenic potato and its immunogenicity in mice
Ahn, Dong-Joo ; Youm, Jung Won ; Kim, Suk Weon ; Yoon, Won Kee ; Kim, Hyoung Chin ; Hur, Tai-Young ; Joung, Young Hee ; Jeon, Jae-Heung ; Kim, Hyun Soon ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 217~224
DOI : 10.14405/kjvr.2013.53.4.217
Transgenic plants have been tested as an alternative host for the production and delivery of experimental oral vaccines. Here, we developed transgenic potatoes that express the major antigenic sites A and D of the glycoprotein S from transmissible gastroenteritis coronavirus (TGEV-
) under three expression vector systems. The DNA integration and mRNA expression level of the TGEV-
gene were confirmed in transgenic plants by PCR and northern blot analysis. Antigen protein expression in transgenic potato was determined by western blot analysis. Enzyme-linked immunosorbent assay results revealed that based on a dilution series of Escherichia coli-derived antigen, the transgenic line P-2 had TGEV-
protein at levels that were 0.015% of total soluble proteins. We then examined the immunogenicity of potato-derived TGEV-
antigen in mice. Compared with the wild-type potato treated group and synthetic antigen treated group, mice treated with the potato-derived antigen showed significantly higher levels of immunoglobulin (Ig) G and IgA responses.
Alternatively activated M2 macrophages increase in early stages of experimental autoimmune myocarditis in Lewis rats
Oh, Hanseul ; Ahn, Meejung ; Matsumoto, Yoh ; Shin, Taekyun ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 225~230
DOI : 10.14405/kjvr.2013.53.4.225
To better understand the role of macrophages in early stages of experimental autoimmune myocarditis (EAM), we compared the expression of inducible nitric oxide synthase (iNOS) and arginase-1, markers for classically activated M1 and alternatively activated M2 macrophages, respectively, in the hearts of EAM-affected and control rats. Immunohistochemical evidence revealed that both iNOS-positive and arginase 1-positive macrophages were found in EAM lesions, while some cells were co-localized with both markers. This finding suggests that the increased level of arginase-1, which is partly from M2 macrophages, contributes to the modulation of EAM, possibly through the reduction of nitric oxide in the lesion.
Attraction effect against Lycorma delicatula, antioxidant activity and local irritation test of Ailanthus altissima extract
Lee, Seung-Jin ; Park, Seung-Chun ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 231~237
DOI : 10.14405/kjvr.2013.53.4.231
This study aimed to assess the attraction effect against Lycorma delicatula and antioxidant activity of hexane, chloroform, butanol and water fraction obtained from Ailanthus altissima methanol extract. The attraction effect of chloroform fraction showed the highest activity (47%) as compared to that of other fractions. In the DPPH radical scavenging activity, methanol and butanol fraction showed higher antioxidant activity than other solvent fractions. From the above results, the potential chloroform fraction was further performed by local irritation test in New Zealand white rabbits. In eye irritation test, chloroform fraction showed moderate irritant at high concentration 0.5 g/site/mL, but there was no eye irritation at low concentration (0.05 g/site/mL). In accordance with the Draize evaluation of skin irritation, the primary irritation index was calculated to 3.3 and 0.68 at high (0.5 g/site/mL) and low concentration (0.05 g/site/mL) causing moderate and mild irritation, respectively. On the basis of this study, Ailanthus altissima chloroform fraction could be safely considered to be a candidate of attractant against Lycorma delicatula.
Souvenir made of tiger part revealed to be Banteng (Bos javanicus): species identification using DNA sequence analysis
Lee, Seo-Jin ; Hyun, Jee Yun ; Min, Mi-Sook ; Lee, Mu-Yeong ; Lee, Hang ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 239~243
DOI : 10.14405/kjvr.2013.53.4.239
Tigers are one of the most endangered species over the world and protected internationally or locally. However, they are still traded illegally for the Traditional East Asia Medicine or souvenirs / mementos as well as fake products. In the present study, to identify the species of the specimen known as the tiger's genitalia that was sold for mementos in China, two approaches of genetic tools and morphological comparisons were applied. On the basis of the entire sequences of mitochondrial cytochrome b gene (1,140 bp), the result of nucleotide comparisons showed that the specimen examined here is matched with banteng, Bos javanicus (Identities = 1,138/1,140; 99%). In addition, the examination of external morphological characters revealed that the genitalia of the specimen is much more similar to the one of cows, not to tigers. The outcomes highlighted that there are still some fake products of tigers traded in market and molecular genetic tools could help identifying species of wildlife products.
Pathologic studies in lymph nodes of pigs infected with porcine circovirus type 2, porcine reproductive and respiratory syndrome virus
Jung, Ji-Youl ; Kim, Jae-Hoon ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 245~251
DOI : 10.14405/kjvr.2013.53.4.245
Porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) have been suspected to have immunosuppressive effects on pigs. To investigate the correlation between these virus infection and the lesions of lymph nodes including sub-mandibular and inguinal lymph node, 44 pigs (PCV2 single, n = 14; PRRSV single, n = 10; PCV2/PRRSV, n = 14; negative control, n = 6) were examined by histopathology and immunohistochemistry. Histopathologically, granulomatous lymphadenitis characterized by lymphoid depletion with histiocytic cells infiltration was observed in PCV-2 single and PCV-2/PRRSV group. Immunohistochemically, there were significant reduction of B and T lymphocytes in lymph nodes of these groups, while the number of macrophages was increased. In only PRRSV infected group, germinal center hypertrophy and lymphoid necrosis were observed. Immunohistochemically, the number of CD3+ T lymphocytes was slightly increased. Severe lymphocytic depletion in PCV-2 infection-related lymph nodes might be associated with producing immunocompromised state in pig. Comparing with PCV-2 infected group, PRRSV produced minor effects on the changes in immune cell population in the lymph nodes of pigs. PRRSV may increase susceptibility of the disease in pigs by disruption of the first defense lines in target organs, such as the alveolar macrophages in lungs.
Minocycline as a treatment of dog with calcinosis cutis
Jang, Hye-Jin ; Kang, Min-Hee ; Sur, Jung-Hyang ; Park, Hee-Myung ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 253~256
DOI : 10.14405/kjvr.2013.53.4.253
An 8-year-old, castrated male, Schnauzer dog was presented for evaluation of gradually worsening erythematous papules. Physical examination revealed multiple erythematous papules having a firm, gritty texture located in bilateral ears, dorsal midline, perianal and inguinal area. Skin biopsy revealed aberrant structure, characterized by atrophic epidermal-dermal layer structure with granular materials which was presumed as calcinosis cutis secondary to iatrognic hyperadrenocotricism. By initiating minocycline for 14 days, there was reduction in size, number of calcium deposit with remarkably decreased erythema. This case report presents the clinical trial of minocycline as a potential agent in treating dogs with calcinosis cutis.
Palmar annular ligament desmitis in horses: retrospective study between desmotomy and desmectomy techniques
Escodro, Pierre Barnabe ; Lopes, Priscila Faria Rosa ; Bruhn, Fabio Raphael Pascoti ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 257~261
DOI : 10.14405/kjvr.2013.53.4.257
This study evaluated two different surgical techniques for the treatment of palmar annular ligament desmitis (PAL) in horses. The group 1 (G1) consisted of 11 Thoroughbred submitted to desmotomy, and G2 of 22 horses that underwent PAL desmectomy. There was no statistically significant difference between mean palmar annular ligament thickness in both groups evaluated (p = 0.800). In this study, the horses of G1 had 12 times more chance to return to physical activity, when compared to animals of G2 (p = 0.033; O.R. = 12.0; C.I.95% = 1,142 - 126,122). Desmectomy was more efficient in promoting the resolution of the injury and the return to sports activity.
Musical murmur in a dog with acute chordae tendineae rupture
Min, Sa-Hee ; Kang, Min-Hee ; Park, Hee-Myung ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 263~264
DOI : 10.14405/kjvr.2013.53.4.263
A 6 year-old, spayed female, Maltese dog was presented with precordial thrill and mild coughing. Thoracic auscultation revealed a grade V/VI systolic murmur with maximal intensity over the left apex characterized by musical murmur. Echocardiography revealed mild myxomatous degeneration of mitral valve and ruptured chordae tendineae. Musical murmur was produced due to the vibration of ruptured piece of chordae tendineae along with regurgitant flow. After treatment with furosemide and ramipril, clinical signs resolved and precordial thrill reduced. This case report describes typical clinical signs and phonocardiogram of musical murmur in a dog with acute chordae tendineae rupture.
Brainstem auditory evoked potential findings in a French bulldog with bilaterally congenital sensorineural deafness
An, Daegi ; Jung, Dong-In ; Kim, Ha-Jung ; Kang, Ji-Houn ; Chang, Dong-Woo ; Yang, Mhan-Pyo ; Kang, Byeong-Teck ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 265~267
DOI : 10.14405/kjvr.2013.53.4.265
A 3-month-old, intact male French bulldog was suspected of deafness. The dog was irresponsive to environmental noises generated out of sight, but normal responses were noted for visual stimuli. No abnormalities were observed on the neurological, otoscopic, radiographic, and blood examinations. To diagnose the apparent deafness, brainstem auditory evoked potential (BAEP) was recorded in the presented dog together with a normal dog. While the BAEP from the control dog showed a normal wave consisting of 5 peaks, absence of all peaks was noted in the suspected deaf dog. Therefore the dog was definitively diagnosed as bilaterally congenital sensorineural deafness.
Devastating endosulfan poisoning in Korean native cattle
Lee, Bo-Ram ; Lee, Hyun-Kyoung ; Lee, Kyung-Hyun ; Yoon, Soon-Seek ; Kim, Meekyung ; Park, Jung-Won ; Jeong, Seon-Hyang ; Lee, Myoung-Heon ; Bae, You-Chan ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 269~271
DOI : 10.14405/kjvr.2013.53.4.269
Here, we report the poisoning case of 10 cows. Several distinct clinical signs such as convulsion, excessive salivation, circling, lateral recumbency, and death were observed. Necropsy and histopathological examination did not reveal any significant abnormal findings. Moreover, no bacteria or viruses were detected in tissue, blood, and feeding food. However, endosulfan was detected from the stomach contents and microbials. Our results strongly suggest that death of cows may be closely associated with endosulfan poisoning.
Case report of Johne's disease in Korean black goat (Capra hircus aegagrus)
Rhyoo, Moon-Young ; Jung, Ji-Youl ; Lee, Kyung-Hyun ; Her, Ji-Woong ; Bae, You-Chan ; Lee, Hyun-Kyoung ; Baek, Kang-Hyun ; Lee, Bo-Ram ; Jean, Young-Hwa ; Kim, Jae-Myung ; Lee, Myoung-Heon ; Yoon, Soon-Seek ;
Korean Journal of Veterinary Research, volume 53, issue 4, 2013, Pages 273~276
DOI : 10.14405/kjvr.2013.53.4.273
Two Korean black goat (approx. 2 and 3 years old) showing diarrhea and chronic weight loss were submitted to Animal and Plant Quarantine Agency. At necropsy, there were thickening of small intestine and enlargement of mesenteric lymph nodes. Microscopically, they had granulomatous enteritis in the small and large intestine and granulomatous lymphadenitis. By polymerase chain reaction (PCR) and acid fast stain, strong positive reaction and acid-fast rod bacteria were detected. According to the result of histopathology and PCR, we confirmed this case as Johne's disease. As far as we know, this is the first report of Johne's disease in Korean black goat.