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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Research
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Science
Editor in Chief :
Volume & Issues
Volume 54, Issue 4 - Dec 2014
Volume 54, Issue 3 - Sep 2014
Volume 54, Issue 2 - Jun 2014
Volume 54, Issue 1 - Mar 2014
Selecting the target year
Serological monitoring on brucellosis in livestock of Korea
Sung, So-Ra ; Kim, Ji-Yeon ; Her, Moon ; Lee, Kichan ; Kang, Sung-Il ; Lee, Hyang-Keun ; Cho, Hyo Rim ; Lee, Jin Ju ; Jung, Suk Chan ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 197~201
DOI : 10.14405/kjvr.2014.54.4.197
In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.
Stimulatory effects of Bordetella bronchiseptica antigen on bone marrow cells and immune memory responses
Yim, Seol-Hwa ; Joo, Hong-Gu ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 203~208
DOI : 10.14405/kjvr.2014.54.4.203
Bone marrow is a hematological and immunological organ that provides multiple immune cells, including B lymphocytes, and thus plays a critical role in the efficacy of vaccine. We previously demonstrated that Bordetella (B.) bronchiseptica antigen has high immunogenicity in spleen cells, a peripheral immune organ. In this study, we investigated the immunogenicity of B. bronchiseptica antigen in bone marrow cells, a central immune organ. B. bronchiseptica antigen increased the cellular activity of bone marrow cells and significantly enhanced the production of nitric oxide, IL-6, and TNF-
. Bone marrow cells primed with B. bronchiseptica antigen in vivo were harvested and stimulated with the same antigen in vitro. The stimulation of B. bronchiseptica antigen significantly increased the cellular activity and proliferation rate of the primed cells. B. bronchiseptica antigen also greatly induced the production of antigen-specific antibody in the primed cells. Taken together, the present study demonstrated that B. bronchiseptica antigen can stimulate bone marrow cells, a central immune organ, and recall the immune response of the primed bone marrow cells.
Increased expression of galectin-9 in experimental autoimmune encephalomyelitis
Cho, Jinhee ; Bing, So Jin ; Kim, Areum ; Yu, Hak Sun ; Lim, Yoon-Kyu ; Shin, Taekyun ; Choi, Jonghee ; Jee, Youngheun ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 209~218
DOI : 10.14405/kjvr.2014.54.4.209
Experimental autoimmune encephalomyelitis (EAE), an animal model of human multiple sclerosis (MS), reflects pathophysiologic steps in MS such as the influence of T cells and antibodies reactive to the myelin sheath, and the cytotoxic effect of cytokines. Galectin-9 (Gal-9) is a member of animal lectins that plays an essential role in various biological functions. The expression of Gal-9 is significantly enhanced in MS lesions; however, its role in autoimmune disease has not been fully elucidated. To identify the role of Gal-9 in EAE, we measured changes in mRNA and protein expression of Gal-9 as EAE progressed. Expression increased with disease progression, with a sharp rise occurring at its peak. Gal-9 immunoreactivity was mainly expressed in astrocytes and microglia of the central nervous system (CNS) and macrophages of spleen. Flow cytometric analysis revealed that
cells were dramatically increased in the spleen at the peak of disease. Increased expression of tumor necrosis factor (TNF)-R1 and p-Jun N-terminal kinase (JNK) was observed in the CNS of EAE mice, suggesting that TNF-R1 and p-JNK might be key regulators contributing to the expression of Gal-9 during EAE. These results suggest that identification of the relationship between Gal-9 and EAE progression is critical for better understanding Gal-9 biology in autoimmune disease.
Characteristic of bacterial flora from the uterus in HanWoo cattle
Kim, Kiju ; Park, Soyeon ; Cho, Youngjae ; Jung, Bae-Dong ; Park, Joung-Jun ; Hahn, Tae-Wook ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 219~224
DOI : 10.14405/kjvr.2014.54.4.219
Uterine sterilization is important for improving fertility in cattle. This study compared bacterial flora in the uterus between healthy and repeat breeder cows (RBCs). The uterine flushing of six heifers, 13 healthy HanWoo cows and eight RBCs (HanWoo) were sampled, and 15 frozen semen samples were selected. Overall, 35 bacteria were identified from in HanWoo uterine flushing and semen. The bacterial genera identified from HanWoo uterine flushing were Alloiococcus, Bacillus, Enterobacter, Enterococcus, Erysipelothrix, Gardnerella, Granulicatella, Kocuria, Pantoea, Pasteurella, Rothia, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The bacterial genera identified from HanWoo semen were Bacillus, Escherichia, Kocuria, Oligella, Pseudomonas, Serratia, Sphingomonas, Staphylococcus, Stenotrophomonas and Streptococcus. The prevalence and presence of the identified bacteria between healthy cows and RBCs differed significantly. Further studies are needed to determine the role of these bacteria in the uterus of HanWoo cattle with reproductive disorder.
Localization of Klotho in cisplatin induced acute kidney failure
Park, So-Ra ; Kim, Tae-Won ; Kim, Young-Jung ; Kim, Hyun-Tae ; Ryu, Si-Yun ; Jung, Ju-Young ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 225~231
DOI : 10.14405/kjvr.2014.54.4.225
Klotho deficiency is an early event in acute kidney injury (AKI) that exacerbates acute kidney damage. The present study explored the expression of Klotho and inflammation related factors in cisplatin-induced AKI. Rats (n = 18) were treated with cisplatin intraperitoneal injection (5 mg/kg) or left untreated as controls (n = 6), then sacrificed at 5 (n = 6) and 10 days (n = 6) treatment. Five days after cisplatin injection, the serum kidney enzymes and kidney cell apoptosis were significantly increased. Moreover, the expression of Klotho was decreased when compared to the control group, especially in the cortex and outer medulla regions. In contrast, inflammation related signals including nuclear factor kappa B, tumor necrosis factor-
, and tumor necrosis factor-like weak inducer of apoptosis were enhanced. However, 10 days after cisplatin injection, Klotho expression was enhanced upon both IHC and Western blot analysis, with slightly recovered renal function and decreased apoptosis. Furthermore, inflammation related signals expression was decreased relative to the 5 days group. Overall, this study confirmed the opposite expression patterns between Klotho and inflammation related signals and their localization in cisplatin-induced AKI kidney.
Effect of medicinal plant extract for hangover relief
Hyun, Chang-Su ; Park, Garyoung ; Oh, Young Mi ; Lee, Youngjae ; Han, Chang-Hoon ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 233~238
DOI : 10.14405/kjvr.2014.54.4.233
The present study was performed to evaluate the effect of medicinal plant extract on relieving hangovers in mice administered alcohol. The animals were divided into three groups. Each group was treated with fermented plant extract, non-fermented plant extract, or water 30 min after consuming ethanol (2 mL/kg). A locomotor activity test showed that all groups had decreased motor activity until 40 min after plant extract administration. The mice treated with water had lower motor activity until 100 min post-administration. However, the group treated with non-fermented plant extract showed increased motor activity 40 min post-administration, and the higher activity level was maintained until 120 min post-administration. The animals treated with fermented plant extract had a level of motor activity between those of the groups treated with water or non-fermented plant extract. Blood was collected from each mouse 120 min post-administration and aldehyde concentration was measured. The group treated with non-fermented plant extract had a significantly higher (p < 0.05) aldehyde concentration than the other groups. These results demonstrate that the non-fermented medicinal plant extract helped alleviate hangovers 40 min after administration by reducing aldehyde concentrations in the blood.
Serological prevalence of brucellosis of cattle in selected dairy farms in Bangladesh
Hassan, Abdullah Al ; Uddin, M. Bashir ; Islam, Md. Rafiqul ; Cho, Ho-Seong ; Hossain, Md. Mukter ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 239~243
DOI : 10.14405/kjvr.2014.54.4.239
This study was conducted to investigate the status of brucellosis in dairy cattle from five selected dairy farms in the Mohammadpur Beribadh area of Bangladesh. A cross-sectional study was carried out from October 2010 to March 2011 in which a total of 334 serum samples from cattle in five herds were screened by the Rose-Bengal plate-agglutination test (RBPT) and the positives were confirmed using an indirect enzyme-linked immunosorbent assay (I-ELISA). A structured questionnaire was used to collect epidemiological information describing the animals. Overall, 4.20% of the animals were RBPT positive, while subsequent confirmatory tests with I-ELISA revealed that the overall animal-level prevalence derived from the samples was 1.20%. Additionally, the prevalence was relatively higher in females than in males. A significant association was found between abortion, age of the animals, and the occurrence of brucellosis (p < 0.05). Considering the overall low prevalence of brucellosis in the selected farms in the present study, a brucellosis eradication program for dairy farms using a test-and-slaughter policy would be possible.
Evaluation of thermally cross-linked superparamagnetic iron oxide nanoparticles for the changes of concentration and toxicity on tissues of Sprague-Dawley rats
Hue, Jin Joo ; Lee, Hu-Jang ; Jon, Sangyong ; Nam, Sang Yoon ; Yun, Young Won ; Kim, Jong-Soo ; Lee, Beom Jun ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 245~252
DOI : 10.14405/kjvr.2014.54.4.245
This study was investigated the change of concentration and toxicity of thermally cross-linked superparamagnetic iron oxide nanoparticles (TCL-SPION) on tissues of Sprague-Dawley rats. TCL-SPION at the dose of 15 mg/kg body weight was intravenously injected into the tail vein of the male Sprague-Dawley rats. The fate of TCL-SPION in serum, urine and tissues was observed during 28 days. Serum iron level was maximal at 0.25 h post-injection and gradually declined thereafter. In addition, the sinusoids of liver and the red pulp area of spleen were mainly accumulated iron from 0.5 h to 28-day post-injection. In kidney, iron deposition was detected in the tubular area until 0.5 h after injection. Malondialdehyde concentration in the liver slightly increased with time and was not different with that at zero time. In the liver and spleen, TNF-
and IL-6 levels of TS treated with TCL-SPION were not different with those of the control during the experimental period. From the results, TCL-SPION could stay fairly long-time in certain tissues after intravenous injection without toxicity. The results indicated that TCL-SPION might be useful and safe as a contrast for the diagnosis of cancer or a carrier of therapeutic reagents to treat diseases.
Epitheliogenesis imperfecta in a bovine fetus of Korean native cattle
Rhyoo, Moon-Young ; Jung, Ji-Youl ; Her, Ji-Woong ; Lee, Myoung-Heon ; Ku, Kyung-Nyer ; Choi, Kwon-Rac ; Yoon, Soon-Seek ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 253~256
DOI : 10.14405/kjvr.2014.54.4.253
In this study, we describe a case of epitheliogenesis imperfecta (EI) observed in the fetus of Korean native cattle. The fetus had multifocal areas of skin defect, especially on the distal portions of the four limbs, and the affected areas were bright-red and glistening. Histopathologically, these areas were characterized by complete absence of squamous epithelium, infiltration of inflammatory cells into the dermis, atrophy of hair follicles, sebaceous and sweat glands. To the best of our knowledge, this is the first report of epitheliogenesis imperfecta in Korean native cattle.
Fatal cryptosporidiosis in a calf
Baek, Kang-Hyun ; Lee, Hyun-Kyoung ; Lee, Kyung-Hyun ; Kim, Ha-Young ; Park, Jung-Won ; Lee, Bo-Ram ; Her, Ji-Woong ; Lee, Myoung-Heon ; Bae, You-Chan ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 257~260
DOI : 10.14405/kjvr.2014.54.4.257
A calf suffering from diarrhea was admitted to the Animal and Plant Quarantine Agency for diagnostic evaluation. Postmortem examination revealed that the mesenteric lymph node was enlarged and small intestine wall was thin. Microscopically, a large number of small round organisms were attached to the small intestine villi. Villous atrophy and proprial neutrophil infiltration were also observed. Based on modified Ziehl-Neelsen staining, electron microscopy, and ELISA results, the calf was diagnosed with fatal cryptosporidiosis.
Cricopharyngeal achalasia in an old dog
Im, Ji-Eun ; Yoon, Hun-Young ; Kim, Seung-Gon ; Lee, Chang-Min ; Eom, Ki-Dong ; Park, Hee-Myung ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 261~263
DOI : 10.14405/kjvr.2014.54.4.261
A 10-year-old castrated male papillon presented with nasal discharge, coughing and chronic dysphagia. On physical examination, the dog exhibited sneezing, gurgling and movement of the throat with repeated attempts to swallow fluids. A diagnosis of cricopharyngeal achalasia (CPA) was made based on video fluoroscopic demonstration of failure of relaxation of the upper esophageal sphincter. This report describes the diagnosis of CPA in an old dog, which is rarely diagnosed in older animals.
Metastatic lipid-rich mammary carcinoma in a dog
Kim, Myung-Chul ; Kim, So-Yeon ; Lee, Su-Hyung ; Kim, Dae-Yong ; Yoon, Jung-Hee ; Kim, Wan Hee ; Lee, Jeong-Ha ; Kim, Yongbaek ;
Korean Journal of Veterinary Research, volume 54, issue 4, 2014, Pages 265~268
DOI : 10.14405/kjvr.2014.54.4.265
An adult female dog was presented for evaluation of mammary gland masses. Complete blood count and serum chemistry data were within normal limits. Fine-needle aspiration cytology of the mammary masses revealed clusters of malignant epithelial cells with clear cytoplasmic vacuoles. Based on histopathological findings, a diagnosis of lipid-rich mammary carcinoma was made. Approximately 5 weeks after surgical removal, the tumor recurred at the surgery site and metastasis to the tibia was detected. Due to the poor prognosis and deterioration of the condition, the dog was euthanized.