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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Research
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Science
Editor in Chief :
Volume & Issues
Volume 55, Issue 4 - Dec 2015
Volume 55, Issue 3 - Sep 2015
Volume 55, Issue 2 - Jun 2015
Volume 55, Issue 1 - Mar 2015
Selecting the target year
Korean native calf mortality: the causes of calf death in a large breeding farm over a 10-year period
Kim, Ui-Hyung ; Jung, Young-Hun ; Choe, Changyong ; Kang, Seog-Jin ; Chang, Sun-Sik ; Cho, Sang-Rae ; Yang, Byung-Chul ; Hur, Tai-Young ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 75~80
DOI : 10.14405/kjvr.2015.55.2.75
Calf losses have an economic impact on larger Korean native cattle (KNC) breeding farms due to replacement, productivity, and marketing. However, little research on KNC calf mortality or causes of calf death on large-scale breeding farms has been conducted. Based on medical records and autopsy findings from the Hanwoo experimental station of the National Institute of Animal Science, calf death records from 2002 to 2011 were used to identify the causes of mortality. Mortality rate of KNC calves was 5.7%. Large differences (1.8~12.6%) in yearspecific mortalities were observed. Calf deaths were due to digestive diseases (68.7%), respiratory diseases (20.9%), accidents (6.0%), and other known diseases (2.2%). The main cause of calf death was enteritis followed by pneumonia, rumen indigestion, and intestinal obstruction. The greatest number of calf deaths occurred during the fall followed by summer. These results indicated that enteritis and pneumonia were the main reasons for calf death. However, autopsy findings demonstrated that other factors also caused calf death. This study suggested that seasonal breeding and routine vaccinations are the most important factors for preventing calf death, and improving calf health in high land areas with low temperature.
Selection and immunomodulatory evaluation of lactic acid bacteria suitable for use as canine probiotics
Park, Su-Min ; Park, Ho-Eun ; Lee, Wan-Kyu ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 81~88
DOI : 10.14405/kjvr.2015.55.2.81
This study was conducted to isolate lactic acid bacteria (LAB) from dog intestine and identify potential probiotic strains for canine use. One hundred and one LAB were isolated from feces of 20 healthy dogs. Acid, bile, and heat resistance along with adherence to Caco-2 cells and antimicrobial activity against pathogens were examined. To analyze immunomodulative effects, the production of nitric oxide (NO), TNF-
, and IL-
was measured using RAW 264.7 macrophages. Additionally, RAW BLUE cells were used to evaluate nuclear factor-
) generation. Ultimately, three strains were selected as canine probiotics and identified as Lactobacillus reuteri L10, Enterococcus faecium S33, and Bifidobacterium longum B3 by 16S rRNA sequence analysis. The L10 and S33 strains showed tolerance to pH 2.5 for 2 h, 1.0% Oxgall for 2 h, and
for 5 min. These strains also had strong antimicrobial activity against Escherichia coli KCTC 1682, Salmonella Enteritidis KCCM 12021, Staphylococcus aureus KCTC 1621, and Listeria monocytogenes KCTC 3569. All three strains exerted better immunomodulatory effects than Lactobacillus rhamnosus GG (LGG), a well-known commercial immunomodulatory strain, based on NO, NF-
, and TNF-
production. These results suggested that the three selected strains could serve as canine probiotics.
Discovery of antigens for early detection of Mycobacterium avium subsp. paratuberculosis and analysis of characteristics using bioinformatics tools
Park, Hong-Tae ; Park, Hyun-Eui ; Shin, Min-Kyoung ; Cho, Yong-Il ; Yoo, Han Sang ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 89~95
DOI : 10.14405/kjvr.2015.55.2.89
Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is one of the most widespread and economically important diseases in cattle. Current diagnostic methods are based on the detection of anti-MAP antibodies in serum or isolation of the causative agent. However, these techniques are often not applicable for cases of subclinical infection due to relatively low sensitivity. Therefore, finding new antigen candidates that strongly react with the host immune system had been attempted. To effectively detect infection during the subclinical stage, several antigen candidates were selected based on previous researches. Characteristics of the selected antigen candidates were analyzed using bioinformatics-based prediction tools. A total of nine antigens were selected (MAP0862, MAP3817c, MAP2077c, MAP0860c, MAP3954, MAP3155c, MAP1204, MAP1087, and MAP2963c) that have MAP-specific and/or high immune responses to infected animals. Using a transmembrane prediction tool, five of the nine antigen candidates were predicted to be membrane protein (MAP3817c, MAP3954, MAP3155c, MAP1087, and MAP1204). Some of the predicted protein structures identified using the I-TASSER server shared similarities with known proteins found in the Protein Data Bank database (MAP0862, MAP1204, and MAP2077c). In future studies, the characteristics and diagnostic efficiency of the selected antigen candidates will be evaluated.
Performance assessment and improvement plan of the regulatory management system of veterinary medical devices in Korea
An, Hyo-Jin ; Yoon, Hyang-Jin ; Kim, Chung-Hyun ; Wee, Sung-Hwan ; Moon, Jin-San ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 97~103
DOI : 10.14405/kjvr.2015.55.2.97
In this study, the Korean veterinary medical devices management system was evaluated relative to systems in the USA, EU, and Japan. Veterinary medical devices are regulated in Korea based on the Medical Appliance Act of 1997. This was initially supervised by the Ministry of Agriculture, Food and Rural Affairs and Korea Animal Health Products Association, and subsequently by the Animal and Plant Quarantine Agency (QIA) in 2000. These devices were classified approximately 1,400 categories as instruments, supplies, artificial insemination apparatus, and other categories. Each of these devices was assigned to four regulatory grades by the QIA in 2007. The ranking system for veterinary medical devices was implemented in 2014 with 820 products from 162 companies registered by that year. However, in vitro diagnostic devices (IVDDs) for animals were managed as medical devices and biological medicine. In vitro diagnostic reagents for treating infection diseases are not subjected to either a classification or grading system. Veterinary medical devices are currently exempt from good manufacturing practices (GMP) and device tracking requirements. Due to gradual growth of the domestic veterinary medical devices market since 2008, regulation of these devices should be improved with re-examination of IVDDs and GMP certification for the effective operating system.
Identification of Brucella melitensis isolates originating from Mongolia and diagnostic real-time PCR evaluation using a specific SNP
Kang, Sung-Il ; Kim, Ji-Yeon ; Kim, Suk Mi ; Lee, Jin Ju ; Sung, So-Ra ; Kim, Yeon-Hee ; Jung, Suk Chan ; Her, Moon ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 105~110
DOI : 10.14405/kjvr.2015.55.2.105
A real-time PCR assay using hybridization probe (HybProbe) has been developed to detect Brucella (B.) melitensis strains. The primer and HybProbe sets were designed based on the gap gene of chromosome I with a specific single nucleotide polymorphism of B. melitensis. Specificity of the assay was confirmed by comparison to reference Brucella species and other related strains. In the melting curve analysis, B. melitensis generated a peak at
unlike those for other Brucella species observed at
. Sensitivity of the assay for B. melitensis ranged from 20 ng to 200 fg of genomic DNA. The ability to identify 94 Mongolian B. melitensis isolates using the real-time PCR assay was identical to that of classical biotyping methods and differential multiplex PCR. These data showed that this new molecular technique is a simple and quick method for detecting B. melitensis, which will be important for the control and prevention of brucellosis.
Influence of various photoperiods on stress hormone production, immune function, and hematological parameters in ICR mice
Park, Seung-Hyu ; Kim, Il-Gyue ; Kim, Hyung-Chan ; Gang, Mi-Jeong ; Son, Song-Ee ; Lee, Hu-Jang ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 111~116
DOI : 10.14405/kjvr.2015.55.2.111
In the present study, the effects of different photoperiods on stress, immunity, and hematological parameters in ICR mice were evaluated. Fifty male ICR mice 7 weeks old (body weight,
) were divided into five groups: DP-0 (0/24-h light/dark cycle), DP-6 (6/18-h light/dark cycle), DP-12 (12/12-h light/dark cycle), DP-18 (18/6-h light/dark cycle), and DP-24 (24/0-h light/dark cycle). During the experimental period, no significant differences in body weight or feed intake were observed between the groups. Hematological analysis revealed that white blood cell, red blood cell, and hemoglobin values for the DP-0 group were significantly different compared to those of the other groups. After 28 days, no significant difference in serum cortisol concentration was observed among the groups, but serum cortisol levels increased in a light exposure-dependent manner. Total serum immunoglobulin G (IgG) concentrations of the DP-0 and PD-6 groups were significantly increased compared to those of the other groups (p < 0.05), and serum total IgG levels decreased in a light exposure-dependent manner. Results of the present study indicated that various photoperiods affect hematological parameters and total serum IgG levels in ICR mice while having no significant effects on body weight, feed intake, or cortisol levels.
Antibacterial activities of bark extracts from Fraxinus rhynchophylla Hance and Geranium koreanum Kom. against clinical strains of Clostridium perfringens in chickens
Oh, Jae-Young ; Lee, Kwang-Jae ; Wei, Bai ; Roh, Jae-Hee ; Kang, Min ; Cha, Se-Yeoun ; Jang, Hyung-Kwan ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 117~123
DOI : 10.14405/kjvr.2015.55.2.117
Necrotic enteritis (NE) caused by Clostridium (C.) perfringens commonly occurs in domestic broiler farms since antibiotic supplementation in poultry feed has been banned. We evaluated the antibacterial activities of medicinal plant extracts against C. perfringens isolates to select alternative compounds for preventing NE. We compared antibacterial activities using two methods and evaluated susceptibilities of the isolates based on minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). Two (Fraxinus rhynchophylla Hance [FRH] and Geranium koreanum Kom. [GKK]) of the 30 plant extracts had potent antibacterial activities against C. perfringens ATCC 13124 in two assays. The MIC values for FRH and GKK against 20 C. perfringens isolates were
, respectively. The geometric MIC mean values for the two extracts were
, respectively. The MBCs for the two extracts against the same strains were
, respectively. The geometric mean MIC and MBC for GKK were about two-fold lower than those of FRH. The modified spot-on-lawn assay may be useful for measuring primary antibacterial potential. FRH and GKK are expected to be used as feed additives to prevent or treat NE in veterinary practice.
Data analysis for improving population management in animal shelters in Seoul
Cho, Yoon Ju ; Lee, Young-Ah ; Hwang, Bo Ram ; Kim, Hyung Joon ; Han, Jin Soo ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 125~131
DOI : 10.14405/kjvr.2015.55.2.125
A total of 11,395 animals were impounded in shelters in Seoul in 2013. The Animal Protection Division of the Seoul metropolitan government has annual contracts with local veterinary associations as well as Korean animal rescue and management organizations for providing shelter to animals, and collects monthly statistics from these groups. In 2013, the collected intake and outcome data for 25 districts were reviewed to analyze shelter capacity in terms of housing capacity (monthly daily average intake, required holding capacity, and adoption-driven capacity), staff capacity (staff hours required for daily care), and live release rate. Seasonal variations in the monthly daily average intake were observed, indicating that management of these shelters requires various strategies. This study was performed to analyze and interpret meaningful statistics for improving the efficiency of animal shelters in Seoul. However, inconsistent collection of animal statistics limited data compilation. Creation of a basic animal statistics matrix with reference to well-designed matrices from recognized professional animal shelters is essential. These complied statistical data will help plan for future animal shelter needs in Seoul.
Comparison of teratogenecity induced by nano- and micro-sized particles of zinc oxide in cultured mouse embryos
Jung, A Young ; Jung, Ki Youn ; Lin, Chunmei ; Yon, Jung-Min ; Lee, Jong Geol ; Lee, Beom Jun ; Yun, Young Won ; Nam, Sang-Yoon ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 133~139
DOI : 10.14405/kjvr.2015.55.2.133
The increasing uses of zinc oxide nanoparticles (nZnO) in industrial and personal care products raise possible danger of using nZnO in human. To determine whether ZnO induces size-dependent anomalies during embryonic organogenesis, mouse embryos on embryonic day 8.5 were cultured for 2 days under 50, 100, and
of nZnO (< 100 nm) or micro-sized ZnO (mZnO;
), after which the morphological changes, cumulative quantity of Zn particles, and expressions of antioxidant and apoptotic genes were investigated. Although embryos exposed to
of ZnO exhibited no defects on organogenesis, embryos exposed to over
of ZnO showed increasing anomalies. Embryos treated with
of nZnO revealed significant changes in Zn absorption level and morphological parameters including yolk sac diameter, head length, flexion, hindbrain, forebrain, branchial bars, maxillary process, mandibular process, forelimb, and total score compared to the same dose of mZnO-treated embryos. Furthermore, CuZn-superoxide dismutase, cytoplasmic glutathione peroxidase (GPx) and phospholipid hydroperoxidase GPx mRNA levels were significantly decreased, but caspase-3 mRNA level was greatly increased in nZnO-treated embryos as compared to normal control embryos. These findings indicate that nZnO has severer teratogenic effects than mZnO in developing embryos.
Trichophyton mentagrophytes infection in an African lion cub (Panthera leo) and transmission to a zookeeper
Kim, Kyoo-Tae ; Lee, Seung-Hun ; Kwak, Dongmi ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 141~143
DOI : 10.14405/kjvr.2015.55.2.141
Dermatophytosis was found on the right front leg of a 4-month-old female African lion cub (Panthera leo) kept at a zoo with locally marginal alopecia. For diagnosis, culturing on sabouraud dextrose agar was performed and skin scrapings from the lesion were analyzed. The ones from the culture and skin scrapings were identified as Trichophyton mentagrophytes. A zookeeper that had been in contacted with the lion for artificial rearing developed skin lesions with well-defined erythematous plaques on the right arm about 1 month after the lesion in the lion was observed. The ringworm was probably transmitted from the lion through continuous contact.
Congenital systemic melanosis in a black mongrel pig
Jeong, Yea-Ji ; Jung, Ji-Youl ; Her, Ji-Woong ; Baek, Kang-Hyun ; Lee, Jong-Hyeong ; Lee, Myoung-Heon ; Yoon, Soon-Seek ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 145~148
DOI : 10.14405/kjvr.2015.55.2.145
Four 3-day-old piglets with retarded growth were submitted to the Animal and Plant Quarantine Agency for diagnosis. Necropsy showed that one piglet had black spots ranging from 2 mm to 1 cm in diameter in the cerebellum, lungs, regional lymph nodes, and cecum. Histological findings were consistent with the gross appearance in which melanin pigmentation was observed in the organs mentioned above. Based on Fontana-Masson staining, we diagnosed this animal with systemic melanosis. To the best of our knowledge, this is the first case of systemic melanosis in black breeds of swine in Korea.
A case of malignant dysgerminoma in a slaughtered dairy cow
Jung, Ji-Youl ; Her, Ji-Woong ; Jeong, Yea-Ji ; Ku, Kyung-Nyer ; Choi, Kwon-Rac ; So, Byung-Jae ; Yoon, Soon-Seek ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 149~152
DOI : 10.14405/kjvr.2015.55.2.149
An abnormally enlarged right ovary and a mass in fat surrounding the right kidney were discovered in a dairy cow during routine postmortem examination at slaughter. The ovary was dark reddish and multinodular in shape. Numerous cystic structures were identified in the mass. Histopathologically, the ovary was completely replaced with large, uniform, polyhedral neoplastic cells containing vesicular nuclei and prominent nucleoli. The mitotic index was high. In the lymphatic vessels, tumor emboli were observed. Another mass in the fat surranding the right kidney had the same histological features as the ovarian mass. This animal was diagnosed with malignant dysgerminoma and metastasis to other peritoneal organs.
Cervical lung lobe herniation in a Pekingese dog
Choi, Ran ; Suh, Sang-Il ; Hyun, Changbaig ;
Korean Journal of Veterinary Research, volume 55, issue 2, 2015, Pages 153~154
DOI : 10.14405/kjvr.2015.55.2.153
An 8-year-old intact male Pekingese (weighing 13 kg) was presented for evaluation of chronic coughing. Thoracic radiography found a redundant tissue swelling (protruded on expiration and collapsed on inspiration) on the ventral neck just cranial to the thoracic inlet. Fluoroscopy also identified that the cranial portion of the right/left cranial lung lobe was seen to protrude through the thoracic inlet into the ventral neck on expiration. On the echocardiogram, there were no abnormal jets in all cardiac valves and no dilation in all cardiac chambers. Based on diagnostic imaging studies, the case was diagnosed as cervical lung lobe herniation.