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REFERENCE LINKING PLATFORM OF KOREA S&T JOURNALS
> Journal Vol & Issue
Korean Journal of Veterinary Research
Journal Basic Information
Journal DOI :
The Korean Society of Veterinary Science
Editor in Chief :
Volume & Issues
Volume 55, Issue 4 - Dec 2015
Volume 55, Issue 3 - Sep 2015
Volume 55, Issue 2 - Jun 2015
Volume 55, Issue 1 - Mar 2015
Selecting the target year
Evaluation of conservation of endangered species through somatic cell nuclear technology
Hyun, Sang Hwan ; Jeong, Yeon Woo ; Hwang, Kyu Chan ; Lee, Guk Jin ; Yang, Il Suk ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 155~161
DOI : 10.14405/kjvr.2015.55.3.155
The number of wild animal species is gradually decreasing due to poaching, hunting and habitat loss. While several endangered animal species have been successfully preserved at the zoo, assisted reproductive technology (ART) must be applied to restore wild animals. In the case of critically endangered animals, somatic cell cloning is considered the most appropriate method of ART. Somatic cell cloning can be beneficial for the reproduction of endangered species with limited female populations. However, gene and cell banks, and understanding of reproductive physiology and optimization of ART for wild animals are urgently required for further activation of artificial reproduction of endangered species, which enlarges its application and maintains biodiversity. Care should also be taken to consider ethical and legal issues associated with somatic cell cloning for conservation of endangered animals.
Isolation and identification of canine parvovirus type 2b in Korean dogs
Yang, Dong-Kun ; Kang, Kyung-Suk ; Jo, Hyun-Ye ; Kim, Ha-Hyun ; Choi, Sung-Suk ; Song, Jae-Young ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 163~167
DOI : 10.14405/kjvr.2015.55.3.163
Canine parvovirus (CPV) is a major diarrhea-causing agent in puppies. Since CPV type 2 (CPV-2) emerged in 1978, new antigenic variants including CPV-2a, CPV-2b, and CPV-2c have been identified in many countries. Two puppies died suddenly at a veterinary clinic in Gyeonggi province, South Korea. Two viruses were isolated in A72 cells, confirmed as CPV strains based on a CPV rapid kit and an indirect fluorescence test and designated QIACP1403 and QIACP1404. The nucleotide sequences of complete VP2 genes of QIACP1403 and QIACP1404 were determined, and the corresponding amino acid sequences were deduced. Molecular analyses revealed that the QIACP1403 and QIACP1404 isolates were type CPV-2b. Several mutated amino acids were detected on VP2 gene residues of the two isolates. Phylogenetic analyses showed that the two isolates were most closely related to strain CPV-BM11, which was isolated from Chinese dogs in 2011. Our results suggest that these isolates may be a candidate for a vaccine to prevent CPV infection in dogs after conducting passages of the isolates in an in vitro culture system.
Retrospective study of the medical status of 34 Formosan sika deer (Cervus nippon taiouanu) at the Taipei Zoo from 2003 to 2014
Kang, Chu-Lin ; Yu, Jane-Fang ; Lai, Hsueh ; Guo, Jun-Cheng ; Wang, Lih-Chiann ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 169~173
DOI : 10.14405/kjvr.2015.55.3.169
The Formosan sika deer (Cervus nippon taiouanus) is an endemic subspecies in Taiwan. The original wild deer has been extinct since the late 1960s. The largest captive population is located at the Taipei Zoo. Except for infectious disease outbreaks, no systemic medical research has been reported for this subspecies. This study was conducted to analyze the medical status of the captive Formosan sika deer population, including the hematological and serum chemistry characteristics. To accomplish this, medical records for 34 Formosan sika deer from January 2003 to January 2014 were acquired and analyzed. The most common illness and cause of death was trauma, followed by gastrointestinal and respiratory disease, respectively. The hematologic and serum chemical values of healthy adults were quite different from those of sika deer (Cervus nippon yesoensis). This study provides a closer medical understanding of this subspecies and the results will facilitate its management.
Characterization of proteases isolated from Kudoa septempunctata
Shin, Sang Phil ; Zenke, Kosuke ; Yokoyama, Hiroshi ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 175~179
DOI : 10.14405/kjvr.2015.55.3.175
Proteases play important roles in parasite development and host parasite interactions. The protease of Kudoa spp. has been recognized as a key factor of severe proteolysis of fish muscle post-mortem; however, there is little information available regarding the protease of Kudoa (K.) septempunctata, which was recently identified as a cause of food poisoning in humans. The present study was conducted to isolate and characterize proteases to elucidate the type of protease contained in the parasite and determine the optimal pH for protease activity. We confirmed the cysteine protease and metalloprotease produced by K. septempunctata. While the cysteine protease showed optimal activity at pH 5 that decreased rapidly with increasing pH, the optimal activity of metalloprotease was pH 7, and it remained stable from pH 6 to pH 8. These results indicate that the pH of cysteine protease is not proper for fish muscle postmortem, and that metalloprotease can act in human intestines. Overall, the present study provides important information that improves our understanding of the role of protease physiology and the subsequent food poisoning caused by K. septempunctata.
Expression analysis of ciliary rootlet coiled coil protein mRNA during Xenopus development
Rahman, Md. Mahfujur ; Kim, In-Shik ; Ahn, Dong-Choon ; Cho, Ho-Seong ; Kim, Won-Il ; Kim, Bumseok ; Shin, Gee-Wook ; Kwon, Jungkee ; Akanda, Rashedunnabi ; Park, Byung-Yong ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 181~184
DOI : 10.14405/kjvr.2015.55.3.181
Ciliary rootlet coiled coil protein (CROCC), the structural component that originates from the basal body at the proximal end of the ciliary rootlet, plays a crucial role in maintaining the cellular integrity of ciliated cells. In the current study, we cloned Xenopus CROCC and performed the expression analysis. The amino acid sequence of Xenopus laevis was related to those of Drosophila, cow, goat, horse, chicken, mouse and human. Reverse transcription polymerase chain reaction analysis revealed that CROCC mRNA encoding a coiled coil protein was present maternally, as well as throughout early development. In situ hybridization indicated that CROCC mRNA occurred in the animal pole of embryo during gastrulation and subsequently in the presumptive neuroectoderm at the end of gastrulation. At tailbud stages, CROCC mRNA expression was localized in the anterior roof plate of the developing brain, pharyngeal epithelium connected to gills, esophagus, olfactory placode, intestine and nephrostomes of the pronephric kidney. Our study suggests that CROCC may be responsible for control of the development of various ciliated organs.
Serosurveillance and establishment of a reverse transcription-polymerase chain reaction assay for bovine parainfluenza virus type 5
Yang, Dong-Kun ; Choi, Sung-Suk ; Lee, Beom-Joo ; Kim, Ha-Hyun ; Jo, Hyun-Ye ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 185~189
DOI : 10.14405/kjvr.2015.55.3.185
Bovine parainfluenza virus type 5 (bPIV5) was isolated from cattle with downer cow syndrome in 2012, and included both respiratory and neurotropic pathogens from a variety of animals. In the current study, we conducted serosurveillance using sera obtained from seven Korean farms and optimized a reverse transcription-polymerase chain reaction (RT-PCR) assay to detect bPIV5. The overall seropositive rate for Korean cattle was 21.4% (163/760). A farm located near the city of Milyang in Gyeoungnam province had a markedly elevated seropositive rate for bPIV5 compared to that of the other six farms. The regional seropositive rates were 4.2% (8/192) for Haman, 19.5% (18/55) for Hwasung, 73.9% (65/88) for Milyang, 26.0% (50/192) for Namwon, 1.0% (1/96) for Uljin, 13.5% (13/96) for Yeongju, and 32.7% (8/41) for Yongin. The sensitivity and specificity of three RT-PCR primer sets used to amplify the conserved fusion gene of bPIV5 were also evaluated. An RT-PCR assay using the bPIVFR3 primer set was 10-fold more sensitive than the assays using the two other primer sets and did not result in non-specific amplification. These results demonstrated that the bPIFR3 primer set can be used to detect bPIV5.
Virulence factors, antimicrobial resistance patterns, and genetic characteristics of hydrogen sulfide-producing Escherichia coli isolated from swine
Park, Hyun-Eui ; Shin, Min-Kyoung ; Park, Hong-Tae ; Shin, Seung Won ; Jung, Myunghwan ; Im, Young Bin ; Yoo, Han Sang ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 191~197
DOI : 10.14405/kjvr.2015.55.3.191
Escherichia (E.) coli is commensal bacteria found in the intestine; however, some pathogenic strains cause diseases in animals and humans. Although E. coli does not typically produce hydrogen sulfide (
-producing strains of E. coli have been identified worldwide. The relationship between virulence and
production has not yet been determined. Therefore, characteristics of
-producing isolates obtained from swine feces were evaluated including antibiotic resistance patterns, virulence gene expression, and genetic relatedness. Rates of antibiotic resistance of the
-producing E. coli varied according to antibiotic. Only the EAST1 gene was detected as a virulence gene in five
-producing E. coli strains. Genes conferring
production were not transmissible although the sseA gene encoding 3-mercaptopyruvate sulfurtransferase was detected in all
-producing E. coli strains. Sequences of the sseA gene motif CGSVTA around Cys238 were also identical in all
- producing E. coli strains. Diverse genetic relatedness among the isolates was observed by pulsed-field gel electrophoresis analysis. These results suggested that
-producing E. coli strains were not derived from a specific clone and
production in E. coli is not associated with virulence genes.
Anti-inflammatory effects of 4,4'-diaminodiphenyl sulfone (dapsone) in lipopolysaccharide-treated spleen cells: selective inhibition of inflammation-related cytokines
Moon, Sun-Young ; Joo, Hong-Gu ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 199~204
DOI : 10.14405/kjvr.2015.55.3.199
4,4'-diaminodiphenyl sulfone (dapsone) is a sulfone drug that has antibacterial effects on a variety of bacteria, especially Mycobacterium leprae; thus, it has been used to treat leprosy. Previous studies demonstrated that dapsone inhibits integrin-mediated adherence of neutrophils and production of prostaglandin
by polymorphonuclear leukocytes. Hence, dapsone may act in immune cells and regulate cell-mediated inflammation processes. However, its anti-inflammatory effects remain unclear. The present study demonstrated that dapsone modulates the production of inflammation-related cytokines in immune cells. We employed the spleen cells of mice, which are major immune cells, and lipopolysaccharide (LPS) as a causative agent of inflammation for experiments. Dapsone induced a proportional change in splenocyte subsets and the apoptosis of spleen cells. Interestingly, dapsone decreased the production of tumor necrosis factor-alpha and interleukin (IL)-10, but not IL-6, in LPS-treated spleen cells. In other assays, we measured the dapsone-induced production of nitric oxide (NO) and the expression of activation markers of spleen cells. Dapsone decreased NO production in LPS-treated spleen cells. Taken together, our results demonstrate that dapsone has anti-inflammatory effects in immune cells and provide new insight into the potential uses of this agent.
Fecal microbiota analysis of obese dogs with underlying diseases: a pilot study
Park, Hyung Jin ; Lee, Sang Eun ; Kim, Hyeun Bum ; Kim, Jae Hoon ; Seo, Kyoung Won ; Song, Kun Ho ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 205~208
DOI : 10.14405/kjvr.2015.55.3.205
Ten dogs were enrolled in this study: two healthy dogs, two obese dogs without other medical issues and six obese dogs with underlying diseases including pemphigus, chronic active hepatitis, hyperadrenocorticism, narcolepsy, otitis media and heartworm infection. Pyrosequencing of the 16S rRNA gene to explore the gut bacterial diversity revealed that distal gut bacterial communities of samples from patients with pemphigus, otitis media and narcolepsy consisted primarily of Firmicutes, while the major phylum of the distal gut bacterial communities in patients with chronic active hepatitis and hyperadrenocorticism was Fusobacteria. Proteobacteria were the dominant phylum in heartworm infected obese patients.
Hypertrophic cardiomyopathy secondary to severe right and left ventricular outflow tract obstruction in a Maltese dog
Yoon, Won-Kyoung ; Suh, Sang-Il ; Oh, Yeon-Su ; Hyun, Changbaig ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 209~211
DOI : 10.14405/kjvr.2015.55.3.209
An 8-year-old spayed female Maltese (2.5 kg of body weight) presented with the primary complaint of loud heart murmur and exercise intolerance. Diagnostic imaging revealed severe pulmonic stenosis (peak velocity 5.2 m/s) with right ventricular hypertrophy. The dog revisited after 2 years, at which time, diagnostic imaging revealed severe biventricular hypertrophy, dynamic left ventricular outflow tract obstruction, left atrial dilation and pulmonary hypertension with worsened pre-existing pulmonic stenosis. Postmortem investigation revealed hypertrophic cardiomyopathy and regional myocardial infarction. The case was diagnosed as hypertrophic cardiomyopathy secondary to severe right and left ventricular outflow tract obstruction.
Atropine-induced atrial bigeminy during general anesthesia in a Cocker Spaniel dog
Suh, Sang-Il ; Kim, Sung-Eon ; Choi, Ran ; Hyun, Changbaig ;
Korean Journal of Veterinary Research, volume 55, issue 3, 2015, Pages 213~214
DOI : 10.14405/kjvr.2015.55.3.213
A 12-year-old female Cocker Spaniel (7.5 kg of body weight) was presented for resection of a mammary gland tumor. During surgery, the heart rate was remarkably decreased due to a second-degree type I atrioventricular block. Atropine (0.05 mg/kg) was administered to increase the heart rate. Although the heart rate was elevated, atrial bigeminy occurred and persisted until the dog fully recovered from general anesthesia. These results highlight the possibility of atrial bigeminy caused by atropine administration during anesthesia.