In general, a system can be stable when it is designed with a rigid material. However, the use of a rigid system can be limited, such as grasping a glass or using a small surgical instrument. To resolve this limitation, a variable stiffness mechanism was developed using a flexible material. Previous research verified the variable stiffness mechanism where flexible segments and rigid segments were connected alternately in series. However, research into the design parameters of the variable stiffness structure is needed to satisfy the desired stiffness. Therefore, a variable stiffness structure was tested by varying the design parameters to confirm the trend of the stiffness variation. When the radius of the structure becomes larger, the stiffness increases. The stiffness increased with decreasing length of the flexible segments. Under the same design parameters, the length of the flexible segments had a greater effect on the stiffness than the length of the rigid segments. In addition, the stiffness was estimated using the pseudo rigid body model and was compared with the experimental results. This parametric study can be used as a design guideline for designing the variable stiffness mechanism to satisfy the desired stiffness.