• Title, Summary, Keyword: 남조류

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Comparison of strip analysis and HPLC analysis for the quantitative analysis of cyanobacterial toxin (남조류 독소 정량을 위한 스트립분석법과 HPLC 분석법의 비교)

  • Pyo, Dongjin;Yim, Miyeon
    • Analytical Science and Technology
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    • v.28 no.3
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    • pp.168-174
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    • 2015
  • Cyanobacterial toxins, such as microcystins, which exist in Korean lakes, are strongly toxic in fish, cattle, and humans. This study performs a quantitative analysis of cyanobacterial toxins in water by comparing the strip method and the HPLC method. Because the detection ranges of the strip method and the HPLC method are different, the water samples were diluted. The comparison of the strip method and the HPLC method was made using seven samples that contained different concentrations of microcystin. The quantitative results produced by the strip analysis were significantly aligned with the results of the HPLC analysis. The results of correlation analysis were r = 0.99998 and p = 0.00001.

Analysis of Water Quality Variation by Lowering of Water Level in Gangjeong-Goryong Weirin Nakdong River (낙동강 강정고령보 수위저하 운영에 따른 수질 변동특성 분석)

  • Park, Dae-Yeon;Park, Hyung-Seok;Kim, Sung-Jin;Chung, Se-Woong
    • Journal of Environmental Impact Assessment
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    • v.28 no.3
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    • pp.245-262
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    • 2019
  • The objectives of this study were to construct a three-dimensional water quality model (EFDC) for the river reach between Chilgok Weir and Gangjeong-Goryong Weir (GGW) located in Nakdong River, and evaluate the effect of hydraulic changes, such as water level and flow velocity, on the control of water quality and algae biomass. After calibration, the model accurately simulated the temporal changes of the upper and lower water temperatures that collected every 10 minutes, and appropriately reproduced changes in organic matter, nitrogen, phosphorus, and cyanobacteria. However, the simulated values were overestimated for the diatoms and green algae cell density, possibly due to the uncertainties of the parameters associated with algae metabolism and the lack of zooplankton predation function in the simulations. As a result of scenario simulation of running the water level of GGW from EL. 19.44 m to EL. 14.90 m (4.54 m drop), Chl-a and algae cell density decreased significantly.In particular,the cyanobacteria on the surface layer, which causes algal bloom, declined by 56.1% in the low water level scenario compared to the existing management level. The results of this study are in agreement with the previous studies that maintenance of critical flow velocity is effective for controlling cyanobacteria, and imply that hydraulic control such as decrease of water level and residence time in GGW is an alternative to limit the overgrowth of algae.

Diversity of Repetitive Sequences in Toxigenic Cyanobacteria Detected by Repetitive Oligonucleotides-Primed PCR (반복염기 프라이머 PCR에 의해 탐색된 독성 남조류에 분포한 반복염기의 다양성)

  • Koo, Jung-Mo;Yoo, Soon-Ae;Park, Sang-Ho;Choi, Chang-Won
    • Korean Journal of Ecology and Environment
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    • v.33 no.3
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    • pp.206-212
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    • 2000
  • Since some cyanobacterial isolates under selective culturing conditions are lacking of characteristic specialized cells or showing altered morphology, the morpho-taxonomic criteria are not accurate enough to discriminate between species. Instead of morphological parameters, a method based on the single or the combination of repetitive oligonucleotides in a single PCR, repetitive oligonucleotides-primed PCR (ROP-PCR), was applied to generate DNA profiles for members of the cyanobacterial genera Anabaena and Oscillatoria, both of which are responsible for causing poisonous blooms in various freshwater systems. ROP-PCR performed on 10 isolates of the cyanobacteria with ERIC and REP sequences from gram-negative bacteria, STRR1A and LTRR sequences derived from cyanobacterial genome, and eukaryotic repetitive sequences, led to the identification of distinct genotypes, and provided specific and repeatable DNA fingerprints for cyanobacterial isolates. Grouping analysis of cyanobacterial isolates showed a signifiant difference depending on the primer used in PCR.

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Isolation of the Microbes Having Cyanobacteria Lytic Activity from Blooming Reservoirs (수화발생 저수지로부터 남조류 분해능을 가지는 미생물의 분리)

  • 신규철;한명수;최영길
    • Korean Journal of Environmental Biology
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    • v.20 no.1
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    • pp.20-24
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    • 2002
  • We have from water samples of Kwalim, Dochang, and Mulwang reservoirs in Kyonggi-Do, where cyanobacteria blooming occurred. Isolated microbes which have lytic activity for cyanobacteria. Water samples were smeared on the Anabaena cylindrica lawn and incubated in light chamber at $28^\circ{C}$, under 3000 lux for 13 days. A fungus having cyanobacterial lytic activity was isolated from the samples of Dochang reservoir. The isolate was identified as Cryptococcus laurentii by Vitek system. From the culture of the isolate, four major extracellular protein bands (29, 35.2, 40.9, 51.1 kDa) have been detected and the 29 kDa protein band was more thickly appeared in the culture with cyanobacteria.

Influence of Rainfall on Cyanobacterial Bloom in Daechung Reservoir

  • Ahn, Chi-Yong;Kim, Hee-Sik;Yoon, Byung-Dae;Oh, Hee-Mock
    • Korean Journal of Ecology and Environment
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    • v.36 no.4
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    • pp.413-419
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    • 2003
  • The water quality and algal communities in the Daechung Reservoir, Korea, were monitored from summer to autumn in 1999 and 2001. Although the average weekly precipitations during June and July were very similar in 1999 and 2001, they were much different during August and September, the so-called blooming season. The rainfall in 1999 increased about 70% after late August, whereas it decreased to the one-fifth level in 2001. The higher concentrations of chlorophyll- a, phycocyanin, and cyanobacteria were observed in 2001, which resulted in the dense algal bloom. In addition, in 2001, the cyanobacterial percentage remained above 80% during the investigation period, and the cyanobacteria were exclusively composed of Microcystis spp. Conversely, there was no report on the algal bloom in 1999. However, the peak bloom seasons were the same for both years, from late August to early September, irrespective of the amount of precipitation. These results suggest that the magnitude and duration of rainfall before bloom season are important factors determining the extent of cyanobacterial bloom in this system.

Characteristics of Algae Occurrence in Lake Paldang (팔당호의 조류 발생 특성)

  • Kim, Jin-Keun;Lee, Song-Hee;Bang, Ho-Hee;Hwang, Su-Ok
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.5
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    • pp.325-331
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    • 2009
  • This study was performed to evaluate characteristics of algal occurrence in Lake Paldang. Chlorophyll a concentration was highest during spring, based on weekly measurements from 2006 to 2008, which revealed a different trend compared to the data before 2000 that showed the highest level during summer. Total algae cell quantity was also highest during spring, based on monthly data. Overall, Bacillariophyceae was dominant and represented 87% of total algae cells and occupation was more significant during spring and winter. Cyanophyceae was dominant during summer. 2-MIB and geosmin originating in algae which caused odor problems was high in July 2008, and more research on the relationship between algae and production of odor causing material is needed.

Novel Algicidal Substance (Naphthoquinone Group) from Bio-derived Synthetic Materials against Harmful Cyanobacteria, Microcystis and Dolichospermum (유해 남조류 Microcystis와 Dolichospermum에 대하여 선택적 제어가 가능한 생물유래 살조물질 (Naphthoquinone 계열))

  • Joo, Jae-Hyoung;Cho, Hoon;Han, Myung-Soo
    • Ecology and Resilient Infrastructure
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    • v.3 no.1
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    • pp.22-34
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    • 2016
  • We developed a biologically-derived substance naphthoquinone (NQ) derivate for the eco-safe mitigation of harmful cyanobacteria blooms such as Microcystis and Dolichospermum. NQ was reacted with various substituents ($R_n$) to produce different NQ derivatives. We tested a total of 92 algicidal compounds based on the algicidal activity of Microcystis and Dolichospermum. 22 compounds of NQ were selected as candidates (algicidal activity >80% at $1{\mu}M$). Among them, NQ 40 compound showed the highest algicidal activity of 99.6% and 100% at the optimal concentration of $1{\mu}M$ on Microcystis and Dolichospermum, respectively. No algicidal effects of NQ 40 ($1{\mu}M$) were observed against non-target algae such as Stephanodiscus, Cyclotella and Peridinium. According to the results of acute eco-toxicity assessment, the $EC_{50}$ values of NQ 40 compound for Selenastrum capricornutum and Daphnia magna were 3.2 and $14.5{\mu}M$, respectively, and the $LC_{50}$ for Danio rerio was $15.7{\mu}M$. In addition, for D. magna chronic eco-toxicity assessment, no toxicity toward survival, growth and reproduction was observed. Therefore, we suggested the NQ 40 ($1{\mu}M$) compound as an alternative eco-safe algicidal substance to effectively mitigate harmful cyanobacteria blooms.

Decision Algorithm of Natural Algae Coagulant Dose to Control Algae from the Influent of Water Works (정수장 유입조류 전처리를 위한 천연조류제거제(W.H.)의 최적주입농도 결정)

  • Jang, Yeo-Ju;Jung, Jin-Hong;Lim, Hyun-Man;Yoon, Young H.;Ahn, Kwang-Ho;Chang, Hyang-Youn;Kim, Weon-Jae
    • Journal of Korean Society of Environmental Engineers
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    • v.38 no.9
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    • pp.482-496
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    • 2016
  • Algal blooms of cyanobacteria (blue-green Algae) due to the eutrophication of rivers and lakes can cause not only the damage by its biological toxins but also the economic loss in drinking water treatment. The natural algae coagulant, a commercial product known as W.H. containing the algicidal and allelopathic material derived from oak, can control algal problems proactively through the coagulation flotation process. However, because there have been no applications of the process for pre-treatment in drinking water plants, we could find no report on the optimum injection dose of W.H.. In this study, we have conducted several sets of jar-tests while changing W.H. dose and concentration of chl-a for (1) Han-river samples and (2) subcultured cyanobacteria samples, and monitored the removal mechanisms of algae intensively. Based on these jar-test results, two linear equations with variables of chl-a and turbidity have been deduced to predict the optimal W.H. dose after the multiple regression analysis using IBM-SPSS. Also, prototypes of automatic control logic have been suggested to inject the optimal W.H. dose promptly in response to the variation of water quality.

Application of Various Plants as an Inhibitor of Algal Growth: Studies in Barge Enclosure and Artificially Eutrophicated Pond (식물체를 이용한 조류제어 현장 적용성 실험)

  • Kim, Sang-Hun;Jun, Sun-Ok;Lim, Byung-Jin
    • Korean Journal of Ecology and Environment
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    • v.35 no.2
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    • pp.123-132
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    • 2002
  • Experiments have been carried out in barge enclosure near Bukhangang area and arificially eutrophicated pond. Applied plants are three terrestrial plants (Big cone pine, Pine needles and Ginkgo) and one aquatic plant (Water chestnut). In the experiment pond, applied plants are big cone pine, pine needles and ginkgo at the concentration of 0.5 g/l and water chestnut of 0.3 g/l. And it used pine needles at the concentration of 0.3 g/l in barge enclosure. The dominant species was Microcystis aeruginosa. Chlorophyll a concentration decreased to the extent of average 65% as compared with the beginning of experiment by the input of big cone pine and pine needles in artificially eutrophicated pond. But it was decreased to the extent of average 34% by the input of water chestnut. Especially, pine needles significantly inhibited the growth of algae from the early days in barge enclosure, it was declined at a rate of average 74%. It was proved that pine needles was the most inhibitory to the growth of algae at the treatment with $0.3{\sim}0.5\;g/l$, Ginkgo and water chestnut are needed more study. We purified that the dissolved phenolic compounds from the various plants can affect algal growth.

Prediction of cyanobacteria population based on Poisson regression based on hydro-meteorological condition (수문기상 조건을 고려한 Poisson regression 기반의 Cyanobacteria 개체수 예측)

  • Cho, Hemie;Huong, Nguyen Thi;Moon, Jangwon;Kwon, Hyun-Han
    • Proceedings of the Korea Water Resources Association Conference
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    • pp.208-208
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    • 2020
  • 지구온난화와 하천환경의 변화로 수질 오염이 심각해지고 녹조 현상 등의 피해가 증가하고 있다. 특히, 기후변화로 인해 온도와 강우량의 변동성이 동시에 증가하고 있어 하천의 수환경 관리측면에서 어려움이 증가하고 있다. 최근 하천 개발 사업으로 인한 인공 구조물 축조로 하천의 오염도 변화는 중요한 논점으로 대두되었으며, 그에 따라 정확한 수질 전망이 요구되고 있다. 녹조평가에 있어 주요 대리변수(proxy variable)로 chlorophyll-a(Chl-a)가 사용되고 있지만, Chl-a는 규조류와 남조류(cyanobacteria) 모두에서 발견되는 지표로서, 녹조의 유해성을 Chl-a 수질 지표만을 사용하여 판단하기에는 한계가 있다. Chl-a뿐만 아니라 수질에 대한 유량, 온도, 영양염류 등의 영향 또한 기존 연구에서 밝혀진 바 있다. 하지만 기존의 물리기반의 결정론적모형은 수질의 추계학적(stochastic) 특성을 반영하는데 제한적이며, 다양한 수문기상학적 조건을 고려한 시나리오 기반의 분석을 수행하는데 한계가 있다. 따라서 본 연구에서는 특정 지점의 보 건설 이후 수문기상 자료를 이용하여 유해 남조류 개체수와 관계있는 수문기상학적 요인을 평가하고 최종적으로 Bayesian Poisson Regression 기반의 중·장기 녹조 예측 모형을 개발하였으며, 해설결과에 대한 불확실성 정보도 제공할 수 있도록 하였다.

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