• Title, Summary, Keyword: 누에번데기

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Simultaneous Production System of Silkworm Dongchunghacho and Male Pupae Using Both Parent Sex-limited Larval Marking Variety (한성반문잠품종을 이용한 누에동충하초 및 숫번데기의 동시 생산체계)

  • Ji, Sang-Duk;Kim, Nam-Suk;Kang, Pil-Don;Sung, Gyoo-Byung;Hong, In-Pyo;Ryu, Kang Sun;Kim, Young-Ki;Nam, Sung-Hee;Kim, Mi-Ja;Kim, Kee-Young
    • Journal of Sericultural and Entomological Science
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    • v.50 no.2
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    • pp.101-108
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    • 2012
  • This study was conducted to confirm the mass production of male pupae and sex-limited larval marking variety as a host for synnemata production of Isaria tenupes in RDA(Rural Development Administration). Silkworm pupation, infection rate and synnemate formation of I.tenuipes were examined. Among the silkworm varieties tested, male Hansaengjam showed the highest pupation rate at 98.7%. I. tenuipes infection rate of larvae of newly-exuviated 5th instar silkworm was 83.7 ~ 90.4% in the spring rearing season and 91.7 ~ 96.6% in the autumn rearing season. Synnemata production of I. tenuipes was execellent in female Yangwonjam with an incidence rate of 99.5% followed by male Yangwonjam(99.5%) and Baegokjam(99.4%) in the spring and autumn rearing season. Synnemata living weight ranged from 0.93 ~ 1.25 g in the spring rearing season. The female Hansaengjam had the heaviest synnemata weight(1.25 g). Synnemata dry weight ranged from 0.27 ~ 0.35 g in the spring rearing season. The female Yangwonjam had the heaviest synnemata weight(0.35 g).

Pathomorphological Peculiarity of Dometic Silkworm, Bombyx mori. Infected with Cordyceps, Paecilomyces tenuipes (동충하초 감염 누에의 병리형태학적 특성)

  • Yun, Jae-Su
    • The Korean Journal of Pesticide Science
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    • v.13 no.3
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    • pp.177-184
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    • 2009
  • This study was carried out to investigate infection process, symptoms and main component of the domestic silkworm, Bombyx mori. larvae and pupa infected with the entomopathogenic fungus Cordyceps, Paecillomyces tenuipes. The Cordyceps, Paecillomyces tenuipes, was highly infectious to the silkworms. A pathogenicities of Cordyceps, Paecillomyces tenuipes, may be highly virulent because of the low resistance or high susceptibility of the silkworms. The silkworm larva infected with Cordyceps formed phialospores on the phialides at the imperfect stage of the genus Cordyceps, But silkworm pupa infected with Cordyceps formed ascospores in the asci at the perfection stage of the genus Cordyceps. The results of analysis of health silkworm pupa and silkworm pupa infected with Cordyceps were obtained that amino acid, fatty acid, and nucleoside were very different.

Effect of Protease on the Extraction and Properties of the Protein from Silkworm pupa (Protease 처리가 누에번데기 단백질 추출 및 기능성에 미치는 영향)

  • Kwon, Hyo-Jung;Lee, Kyoung-Hwan;Kim, Jeung-Hoan;Chun, Sung-Sook;Cho, Young-Je;Cha, Won-Seup
    • Applied Biological Chemistry
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    • v.49 no.4
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    • pp.304-308
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    • 2006
  • To extract insoluble proteins from silkworm pupa meal, the meal was treated with pretense produced by Bacillus sp. JH-209. The extraction of insoluble silkworm pupa protein was enhanced at alkaline pHs ranged from 7 to 11 by treatment with the protease. The optimum extraction temperature was $40^{\circ}C$ for in soluble protein treated with pretense. The optimum protease treatment time for extraction of protein was 11 hrs and optimum amount of enzyme treated for extraction of protein was 60 Unit, respectively. The treatment of enzyme extracted more protein than ordinary extraction method without pretense. The foaming capacity, foaming stability, emulsion capacity, and emulsion stability of silkworm pupa meal protein extracted by the treatment of the enzymes increased at all pH ranges. Further more oil absorption as well as water absorption capacities of the protein extracted by the treatment of the enzymes were also increased.

Study on analysis of components and artificial cultural practice on several culture media of Paecilomyces japonica (눈꽃동충하초 배지별 인공재배법과 성분분석에 관한연구)

  • 이희덕;김용균;김홍규;이가순
    • Korean Journal of Plant Resources
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    • v.12 no.2
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    • pp.102-106
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    • 1999
  • This experiment was carried out to find the method for mass production by artifical cultivition and to analyze the components of Paecilomyces japonica according to several media. Time of inoculation of the Paecilomyces japonica using silkworm was on first day of five molting and infection rate was 72.0%. Optium medium for mass production of the Paecilomyces japonica was known effective for increasing dry weight and fruitbody at brown rice 80g plus pupa powder 20g. Dry weight of Paecilomyces japonica using fungus of silkworm was 1.2g including pupa and length of fruitbody was appeared 3.0cm to 3.5cm. Content of $\beta$ - glucan was very high as 40.5% at inoculation on the first day of the five molting while 16.4% at brown rice, 20.7% at pupa, 23.1% at brown rice plus pupa powder, and 28.7% at pine sawdust plus wheat bran. Mycelium was poor and pinkly conidiospore was formed on media of centipede, maggot and powder of silkworm.

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The Detection and Multiplicity of Entomopathogenic Nematodes Using Silkworms (Bombyx mori) (누에를 이용한 곤충병원성 선충의 검색 및 배양)

  • 한상미;이광길;여주홍;권해용;우순옥;이용우;백하주;한명세
    • Journal of Sericultural and Entomological Science
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    • v.45 no.2
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    • pp.103-107
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    • 2003
  • Entomopathogenic nematodes were isolated through the investigation in soils collected from cultivated and non-cultivated fields using silkworms (Bombyx mori) and Galleria mellonella trap. The detectable rate of entomopathogenic nematodes of silkworms trap was higher than the G. mellonella trap. This study indicates the detection of entomopathogenic nematodes from soils that silkworms are sensitive superior to the G. mellonella to entomopathogenic nematodes. The steinernema, rhabditidae, and diplogatroidae strains successfully cultured on the silkworms host as well as on artificial media. Reproductivity in the living silkworm larva and pupa was 1.5 to 3.5${\times}$ 10$\^$5/ nematodes per host However, G. mellonella could be multiplied less than 5${\times}$l0$\^$5/ nematodes. The dried pupa of the silkworm following mositurize was cultured 0.5 to 2${\times}$10$\^$5/) nematodes per host. The culture methods of the steinernema, rhabditidae, and diplogatroidae strains, using silkworm powder, extracted chicken intestine, and food waste fertilizer could be applicative, but rate of reproduction was low.

Increased Muscle Mass after Resistance Exercise Training and Ingestion of Silkworm Pupae Powder (Bombyx mori L.) in ICR Mice (누에나방(Bombyx mori L.) 번데기 분말 섭취가 마우스의 저항성운동 훈련 후 근육량 증가에 미치는 영향)

  • Kim, Yiseul;Kim, Heebin;Jeon, Byungduk;Lee, Dong Woon;Ryu, Sungpil
    • Korean journal of applied entomology
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    • v.55 no.1
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    • pp.1-9
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    • 2016
  • Edible insects are an interesting alternative global food resource. The purpose of this study was to investigate whether ingestion of silkworm (Bombyx mori) pupae powder with and without resistance exercise training (isometric contraction training) increased muscle mass in ICR mice. To achieve this, 28 ICR mice were grouped into control (CON), resistance exercise training (EX), silkworm pupae powder ingested control (SP), and silkworm powder ingestion with resistance exercise training (SPEX) groups. The change in body weight ratio was significantly decreased in the EX and SPEX groups compared to the CON and SP groups. Total blood protein levels were the highest in SPEX mice compared to those in other groups. The albumin concentration increased only in the EX group. Blood GOT and GPT levels were not significantly affected. Changes in Akt and Gsk-$3{\beta}$ protein expression were not significant but there was a tendency for Akt to increase and for Gsk-$3{\beta}$ to increase following the ingestion of the powder. The size of the gastrocnemius muscle increased significantly in response to resistance exercise training only. Furthermore, the ingestion of silkworm pupae powder tended to increase muscle mass without significance. These results suggested that the ingestion of silkworm pupae powder with resistance exercise training might enhance muscle mass without hepatotoxicity. However, future study may be needed to obtained detailed results and practical suggestions.

Sex-Hormone Replacement Effect of Silkworm Pupa and Mixture with Herbs (미성숙 흰쥐에서 누에 번데기 및 한약재 혼합물의 여성호르몬 대체효과)

  • Yang Ji-Won;Choi Eun-Mi;Kwon Mu-Gil;Koo Sung-Ja
    • Korean journal of food and cookery science
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    • v.21 no.6
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    • pp.769-775
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    • 2005
  • In this study we isolate substances to serve as dietary resources in order to replace the female hormone. Silkworm (Bombyx mori) is one of the most attractive hosts for large-scale production of eukaryotic proteins which have been proven safe as a dietary resource. We report on the estrogenicity of a mixture of silkworm pupa and herbs (Ginseng,Ulkeum, and Hasuo) using the immature rat uterotrophic assay in vivo. Silkworm pupa aqueousextract (KW) and silkworm oil extract (KO) induced effects on the immature rat uterotrophic assay. KO showed neither positive uterotrophic response nor inhibition on E2 induced effect, while KW and MK (mixture of KW and herbs) showed both of the effects. It is concluded that ethanol extracts from silkworm might be a good, therapeutic, natural product for hormone-deficient diseases.

Investigation of the Condition of Fruiting Body Formation by Cordyceps scarabaeicola (풍뎅이동충하초(Cordyceps scarabaeicola)의 자실체 형성 조건)

  • Lee, Jae-Keun;Sung, Jae-Mo;Park, Young-Joon
    • The Korean Journal of Mycology
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    • v.30 no.1
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    • pp.11-17
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    • 2002
  • This experiment was carried out to study formation of fruitbody with Cordyceps scarabaeicola (EFCC C-252) isolate. This isolate was the one of best fruitbody formation on brown rice 60 g plus 30 g silkworm pupa media among EFCC C-251, EFCC C-252, EFCC C-1092 from EFCC (Entomopathogenic Fungal Culture Collection) of Kangwon National University. Fruiting body was formed only isolate EFCC C-252 among tested isolates on the medium of brown rice (60 g) and silkworm pupae (30 g). The optimal temperature and light for fruiting body formation were $25^{\circ}C$ and fluorescent light (300 lux). The maximal fruiting body formation was observed at 70 g of brown rice and 80 g of silkworm pupa medium which was treated separately. Fruiting body was formed maximally by 2 days interval of irrigation.

Sex-Discrimination of Silkworm Pupa, Bombyx mori with Image Analyzer (화상처리장치에 의한 번데기 암수판별의 효과)

  • ;Tohru Nakada
    • Journal of Sericultural and Entomological Science
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    • v.35 no.2
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    • pp.105-113
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    • 1993
  • To produce F1 hybrid of silkworm sex discrimination has to be followed at the pupal stage by sexual organ. However it requires a lot of labour and may bring about a wrong classification. In these regards, this study has been implemented to find out an effective measure for the pupal sex discrimination by use of variation of cocoon weight and image analysis of cocoon. As a result, it was found that in case of the pupal weight the percentage of a wrong classification fell on 0.3% and in case of single cocoon weight 0.4%. The discrimination rate was 99% in the weight variables of cocoon but analysis by single cocoon weight and cocoon shape variables, it was 98.7%. Efficiency of discrimination was increased by 2.7% as compared to variable of single cocoon weight. The minimum cocoon sampling size may be 15 cocoons sexual-wise.

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