• Title, Summary, Keyword: 병징

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Symptoms of Cucumber Virus Diseases Occurred in Sangju and Gurye in 2006 and 2007 (2006년과 2007년 상주와 구례에서 발생한 오이 바이러스병의 병징 특성)

  • Cho, Jeom-Deog;Lee, Jung-Hwan;Ko, Sug-Ju;Choi, Hong-Soo;Lee, Su-Heon;Choi, Gug-Seoun;Kim, Jeong-Soo
    • Research in Plant Disease
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    • v.17 no.2
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    • pp.196-204
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    • 2011
  • Virus diseases occurring on cucumber was surveyed at main plantation areas of 'Sangju' and 'Gurye' in 2006 and 2007. Viral infection rate on cucumber was ranged from 14% to 90% in fields and the average infection rate was 46% at Sangju area. Cucumber cultivated at Gurye area had viral incidence ranged 9-100% and averaged 48%. The majorly infected viruses were Cucumber green mottle mosaic virus, Zucchini yellow mosaic virus, Papaya ring spot virus and Watermelon mosaic virus-2, and their infection rates were 23.5%, 13.0%, 9.0% and 2.0%, respectively in 2006 and 2007. Mixed infection rate of duplex, triplex and tetraplex was 31.5%, 7.5% and 2.5%, respectively. The rate of infection type was 47.7% by single infection, 31.5% by double infection and 88.7% by both infection types. Important viruses involved in mixed infection types were Zucchini yellow mosaic virus, Cucumber green mottle mosaic virus, Papaya ring spot virus and Broad been wilt virus2, orderly. Symptom in single infection was almost same on 'Dadagi' line and 'Chicheong' line of cucumber cultivars. Cucumber green mottle mosaic virus produced various symptoms of chlorotic spot, vein chlorosis and vein wrinkle and so on. Main symptoms of vein chlorosis, and severe mosaic and malformation were induced by Zucchini yellow mosaic virus. Mild symptoms were occurred relatively by Papaya ring spot virus and Watermelon mosaic virus2.

Histopathological Observation and Identification of Fusarium spp. Causing Soybean Sporut Rot (콩나물 부패를 일으키는 Fusarium spp.의 동정과 병태조직학적 관찰)

  • 오병준;박원목
    • Korean Journal Plant Pathology
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    • v.12 no.4
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    • pp.471-475
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    • 1996
  • 콩나물의 생육부진과 부패는 콩나물 재배에 중요한 제한요인이다. 콩나물 부패 병원 진균으로 Fusarium moniliforme, F. oxysporum과 F. solani를 분리 동정하였다. 이들은 종자 발아 후 3∼5일이 지난후 콩나물의 중간 이하에 연한 갈색의 외부 병징을 만들며 발아 후 6∼7일 경에 병징이 더욱 진전하여 진한 갈색의 외부 병징을 나타냄으로써 콩나물에 대한 병원성이 인정되었다. Hand-section과 microtome-section에 의한 병태 조직학적 관찰에서 F. oxysporum 접종 후 4일이 경과하여 초기 병징을 보인 조직에서 균사가 피층, 세포간극, 목부, 사부, 수에 분포하고 있음이 관찰되었다.

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Identification of Virus from Azuki Bean Plant (팥에 발생하는 바이러스 분리 동정)

  • 허남기;강문석;하건수;김혜자;최장경
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.2
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    • pp.160-165
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    • 1997
  • Three types of virus disease symptoms were observed in azuki bean plants: yellow mosaic; mosaic; severe mosaic with dwarf. The symptoms developed in the indicator plant inoculated with a virus- infected leaf of azuki bean showed similar host range with those of AMV, CMV and AzMV. In antiserum response, yellow mosaic symptom formed sediments with AMV antiserum, mosaic type with CMV antiserum, respectively, From the electron microscope observation, eclliptic particle (18~58$\times$18nm), isometric particle (30nm), and filamentous(730$\times$12nm) combined with inclusion body were observed in yellow mosaic, mosaic, and severe mosaic with leaf curling symptoms, respectively, The results demonstrate that yellow mosaic, mosaic, and severe mosaic with dwarf are caused by AMV, CMV and AzMV.

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Classification of Potato Virus Y Strains based on Reactions of Differential Plants (감자바이러스 Y 계통분류를 위한 판별 식물)

  • Park E. K.;Choi J. K.
    • Korean journal of applied entomology
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    • v.23 no.4
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    • pp.203-208
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    • 1984
  • Four varieties of tobacco, Nicotiana tabacum var. NC 2326. NC 95, NC 744 and Havana. and two plant species, Physalis floridana Rydb., N. repanda Wild. were used for classification of ten isolates of potato virus Y(PVY) obtained Iron potato or tabacco plants showing various symptoms. Each of the 10 isolates could be distinguished by the host reactions showing necrosis, vein banding and/or mottling, or no symptom on these hosts. Five isolates of PVY, PVY-VN, PVY-N, PVY-NSNR, PVY-Chile, and PVY-Argentina, showed necrotic symptom on NC 2326, but others showed vein handing and/or mottling symptom. On NC95, each of the isolates tested showed similar symptons as on NC2326, except necrotic symptom by the isolate PVY-MSNR. Havana showed mottling reaction to the PVY-NSNR, PVY-MSNR, PVY-MSMR, PVY-VB, and necrosis to PVY-Chile, PVY-Argentina, but showed no symptom to the others. NC744 showed mottling symptom to the PVY-MSNR and PVY-MSMR, necrosis to the PVY-Chile and PVY-Argentina, and no symptom to the others. On N. repanda, necrotic reaction to PVY-Argentina. no symptom to PVY-VN and PVY-C, and nettling to others were observed.

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Sooty Leaf Blight of Cymbidium spp. Caused by Pseudocercospora cymbidiicola (Pseudocercospora cymbidiicola에 의한 심비디움 검은잎마름병)

  • Han, Kyung-Sook;Park, Jong-Han;Lee, Jung-Sup;Cheong, Seung-Ryong
    • Research in Plant Disease
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    • v.13 no.2
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    • pp.126-129
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    • 2007
  • Sooty leaf blight disease of Cymbium spp. was observed on orchid fields located at Gyeonggi-do in 2005-2006. Symptoms of the disease appeared on leaves and leaf spots were circular to nearly-circular, these circular blemished were yellow, with greater amounts of brown to black flecks forming as the spots enlarge. Severely infected leaves were dry and defoliated. These symptoms were realized wrongly as symptoms by virus. But Pseudocercospora cymbidiicola were isolated from the diseased plants. Conidiophores were produced on the lesion surface of the leaf with the blemished areas andconidia formed dark brown, cylindrical and straight to slightly curved, 5-9 septate, $23.7-85.0\;{\times}\;2.0-3.4\;{\mu}m$. Mycelial growth was mostly slow on potato dextrose agar and the optimum temperature for growth was $25^{\circ}C$. We were identified as Pseudocercoepora cymbidiicola based on the morphological characteristics.

Cross-Protection Effectiveness of Cucumber mosaic virus (CMV) Isolates Associated with Satellite RNA for Prevention of CMV Disease in Pepper Plants (Satellite RNA 보유 Cucumber mosaic virus(CMV)의 고추 CMV병에 대한 교차방어 효과)

  • 최장경;성미영;정혜진;홍진성;이상용
    • Research in Plant Disease
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    • v.7 no.3
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    • pp.155-163
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    • 2001
  • Two attenuated Cucumber mosaic virus (CMV) isolates, Paf-CMV and Rs2-CMV that had been selected from CMV isolates associated with satellite RNA (satRNA) were tested for cross-protection effect in pepper plants. The viruses selected as attenuated strains appeared to be identical serologically and physically to the challenge virus (Mf-CMV), but they were lower in the dilution end-point of infectivity of crude sap than Mf-CMV When symptoms were observed in several indicator plants after inoculation, Paf-CMV and Rs2-CMV were symptomless or showed mild mosaic symptoms while another satRNA isolate Ap-CMV developed severe mosaic symptoms on the leaves as Mf-CMV. The nucleotide sequences of the satRNAs were determined by sequencing full-length cDNA clones. Paf-, Rs2- and Ap-satRNAs were 386, 335, and 347 nucleotides long, respectively, The sequences were then compared with the other known Y-satRNA, revealing that nucleotide sequences of the satRNAs consisted of 5'- and 3'-terminal conserved regions. However variations occurred on the middle regions of the sequences, especially those related to symptom interference, showing significant differences between Paf-satRNA and other isolates. Infectious transcripts of Paf-satRNA and Rs2-satRNA induced mild mosaic symptoms in pepper plants when supported by genomic RNAs of Mf-CMV. Under greenhouse conditions, Paf-CMV and Rs2-CMV were tested for cross-protection effect in pepper and tobacco (Nicotiana tabacum cv, Xanthi nc) plants against Mf-CMV. No symptoms were developed on the plants vaccinated with Paf-CMV until 3 weeks after inoculation with the virulent strain; however another attenuated isolate, Rs2-CMV, showed less effectiveness in cross-protection. Depending on the concentration of the challenged virus, symptoms sometimes appeared later in the upper leaves. However, in plants challenged with low concentrations (below 0.2 mg/ml) of the challenge inoculum, symptoms caused by the virulent strain did not develop on the plants vaccinated with Paf-CMV. In the field experiments, the number of pepper plants with severe mosaic symptoms in the control plots was progressively increased after transplanting and reached approximately 50% after 50 days. On the other hand, the incidence of mosaic disease appeared very low on the plants that had received the protective inoculation with Paf-CMV.

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Characteristics of Cucumber mosaic virus-VCH Causing Vein Chlorosis on Red Pepper in Korea (고추에 엽맥퇴록병을 일으키는 오이 모자이크 바이러스(CMV-VCH)의 특징)

  • Cho, Jeom-Deog;Lee, Sin-Ho;Kim, Jeong-Soo;Choi, Gug-Seoun;Kim, Hyun-Ran;Chung, Bong-Nam;Ryu, Ki-Hyun
    • Research in Plant Disease
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    • v.12 no.3
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    • pp.226-230
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    • 2006
  • Cucumber mosaic virus(CMV) was occurred on red pepper showing vein chlorosis or vein necrosis with the incidence rate of 52% from 62 specimens collected in natural fields. Among 32 samples infected with CMV, the specimens of 22 red pepper leaves showing vein chlorosis were infected singly with CMV-VCH. CMV-VCH induced vein chlorosis on the inoculated leaves, and vein banding and vein necrosis on the upper leaves of Nicotiana glutinosa, and then killed after showing bud necrosis. The typical symptoms of vein banding, malformation and blister were produced on the upper leaves of Nicotiana benthamiana and N. tabacum 'Ky-57' without symptoms on the inoculated leaves. The commercial cultivars of 'Bugang', 'Manitta' and 'Gwariput' were shown the typical symptom of vein chlorosis by the mechanical inoculation of CMV-VCH. CMV-VCH was detected specifically by RT-PCR. Virus particles of CMV-VCH were isometric shape having 30 nm diameter. Ultraviolet absorption of purified CMV-VCH was maximum at 260 nm and minimum at 242 nm. The ratio of A260/A280 was 1.71. CMV-VCH had the single nucleo-protein having the molecular weight of 24.5 kDa.

Resistance Evaluation of Radish (Raphanus sativus L.) Inbred Lines against Turnip mosaic virus (순무모자이크바이러스에 대한 무 육종 계통 저항성 평가)

  • Yoon, Ju-Yeon;Choi, Gug-Seoun;Kim, Su;Choi, Seung-Kook
    • Research in Plant Disease
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    • v.23 no.1
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    • pp.60-64
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    • 2017
  • Leaves of twenties radish (Raphanus sativus L.) inbred lines were mechanically inoculated with Turnip mosaic virus (TuMV) strain HY to evaluate TuMV resistance of the radish inbred lines. The inoculated radish plants were incubated at $22^{\circ}C{\pm}3^{\circ}C$ and resistance assessment was examined using symptom development for 4 weeks. Based on the reactions of differential radish inbred lines, 16 radish lines were produced mild mosaic, mottling, mosaic and severe mosaic symptoms by TuMV infection. These results were confirmed by RT-PCR analysis of TuMV coat protein gene, suggesting that TuMV is responsible for the disease symptoms. Four resistant radish lines did not induce systemic mosaic symptoms on upper leaves and chlorosis in stem tissues for 4 weeks, showing they were symptomless by 8 weeks. Further examination of TuMV infection in the 4 radish lines showed no TuMV infection in all systemic leaves. These results suggest that the 4 radish lines are highly resistant to TuMV.

Comparative Analysis of Genetic Variation of Cucumber Mosaic Virus from Commelina communis in Korea (국내 닭의장풀에서 분리된 오이모자이크바이러스 분리주들의 외피단백질 유전자와 병징 다양성 비교)

  • Park, Tae-Seon;Hong, Jin-Sung
    • Research in Plant Disease
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    • v.24 no.2
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    • pp.170-173
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    • 2018
  • Three isolates of Cucumber mosaic virus isolated from Commelina communis plants showing chlorosis and mosaic were collected in Chungju and Chuncheon, Korea. To confirm genetic variation of these three isolates (CMV-Co, CMV-Co2, and CMV-Co3), we performed PCR-RFLP and sequence analysis. Sequences of coat protein genes of CMV-C0, -Co2 and -Co3 were compared with CMV-Fny and showed 96.3%, 96.3%, and 95.9% similarities, respectively. In host reactions, three CMV-Co isolates induced systemic necrosis in Cucurbita pepo unlike CMV-Fny and CMV-Co, CMV-Co2 and CMV-Co3 observed differential symptoms responses in Physalis angulata and Nicotiana rustica. These results indicated that three isolates of CMV isolated from C. communis have genetic and biological variation.

Incidences and Causal Agents of Postharvest Fruit Rots in Kiwifruits in Korea (우리나라 참다래 저장병 발병율과 병원균)

  • Koh, Young-Jln;Lee, Jae-Goon;Hur, Jae-Seoun;Jung, Jae-Sung
    • Research in Plant Disease
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    • v.9 no.4
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    • pp.196-200
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    • 2003
  • Sixteen hundred fruits were randomly collected from 16 kiwifruit (Actinidia deliciosa) orchards in Jeonnam, Gyeongnam and Jejn provinces in Korea in 2000 and incidences of postharvest fruit rots were examined. The overall disease incidence was 32% and varied much with locations of orchards ranging from 5 to 68%. The percentages of kiwifruits showing internal, external, and both internal and external symptoms were 21.9%, 4.9%, and 5.2%, respectively. Several fungi were isolated from rotten fruits; Botryosphaeria dothidea, Diaporthe actinidiae and Botrytis cinerea were the major pathogens with the average isolation rates of 83.3%, 11.9% and 1.4%, respectively. Based on the symptoms on kiwifruits and the characteristics, the postharvest fruit rots caused by B. dothidea and D. actinidiae are suggested to be named as ripe rots and stem-end rots, respectively.