• Title, Summary, Keyword: 암세포 증식억제 효과

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Enhancement of Anticarcinogenic Effect by Combination of Sedum sarmentosum Bunge with Platycodon grandiflorum A. Extracts (도라지 추출물 첨가에 의한 돌나물의 항발암 상승효과)

  • 박윤자;김미향;배송자
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.1
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    • pp.136-142
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    • 2002
  • Anticarcinogen is one of the major strategies for cancer control. It is well established that dietary factors play an important role in modulating the development of certain types of human cancer. We investiagted the anticarcinogenic effects of Sedum sarmentosum Bunge (SS) with Platycodon grandiflorum A. extracts on HepG2, HeLa and MCF-7 cell lines. By the MTT assay, among the five partition layers of methanol extract of SS (SSM), the ethylether partition layer of SS (SSMEE) showed the strongest cytotoxic effects on all cell lines. We also investigated the synergistic effect of the combination of SS and PG extracts on growth inhibition of the HepG2, HeLa and MCF-7 cell lines compared to the effects of five partition layers of SSM. Combination of SS and PG extracts significantly increased cytotoxic effects on all cell lines. Therefore, we were able to conclude that ethylether partition layer, SSMEE might have potentially useful cytotoxic materials on all the human cancer cells which we used. And we could suggest that the combination of SS with PG enhanced the anticarcinogenic effect on HepG2, HeLa and MCF-7 cell lines. We also determined QR activity of partition layers of SSM, among them, SSMEE on HepG2 cells showed the highest QR activity, 3.21 as control value of 1.0.

Effect of Mycelia Extracts from Lentinus edodes Mushroom-Cultured Lonicera japonica Thunberg on Anticancer and Antiallergy Activities (인동초로 배양한 표고버섯 균사체 추출물의 항암 및 알레르기 억제효과 검증)

  • Bae, Man-Jong;Ye, Eun-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.4
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    • pp.424-430
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    • 2007
  • This study was conducted to investigate the effect of mycelia of Lentinus edoes mushroom-cultured Lonicera japonica Thunberg (LLJ) on proliferation of the cancer cell lines (Hep3B, MCF-7 and HeLa), sarcoma 180 (S-180) and antiallergy. In an anti-cancer test using Hep3B (hepatic cancer cell), MCF-7 (breast cancer cell) and HeLa (uterine cancer cell), LLJ extract showed higher antiproliferating effect than that of LJ (Lonicera japonica Thunberg) extract. In an anti-cancer testing using Hep3B cells, LLJ extract showed growth-inhibitory effect of $85.60{\pm}4.66%$ at 3mg/rnL. In an anti-cancer testing using MCF-7 cells, LLJ and LJ extracts showed high antiproliferating effect. LLJ showed the tumor suppressive effect in mice injected with S-180 cells. The growth-inhibitory rates against tumor cells were 61% for LLJ, 37% for LJ. LLJ inhibited histamine release from rat peritoneal mast cells activated by compound 48/80. These results suggest that Lentinus edodes mushroom-cultured herb has an antiproliferating effect against cancer cell lines (Hep3B, MCF-7 and HeLa) and S-180 tumor, and will be beneficial in the treatment of allergic reaction.

Inhibitory Effects on Cell Survival and Quinone Reductase Induced Activity of Aster yomena Fractions on Human Cancer Cells (쑥부쟁이 분획물의 in vitro 암세포증식 억제 및 QR 유도효과)

  • Jung, Bok-Mi;Lim, Sing-Sn;Park, Yun-Ja;Bae, Song-Ja
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.1
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    • pp.8-12
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    • 2005
  • This study was performed to determine the inhibitory effects on cell survival and Quinone reductase induced activity of Aster yomena (AY) on human cancer cells which, using methanol, was extracted and fractionated into five different solvent types: hexane (AYMH), ethylether (AYMEE), ethylacetate (AYMEA), butanol (AYMB) and aqueous (AYMA) partition layers. The experiment was conducted to determine cytotoxicity of various Aster yomena partition layers on HepG2, HeLa and MCF-7 cells by MTT assay. Among various partition layers of Aster yomena, A YMEE and A YMEA showed the strong cytotoxic effects on all cancer cell lines we used. The Quinone reductase (QR) induced activity on HepG2 cells, A YMH at a does of 100 $\mu$g/mL was 2.46 times more effective compared to the control value of 1.0.

Antiproliferative Activity of Vegetable Soup in Human Cancer cells for Wellness Convergence (융복합적인 웰리스를 위한 야채수프의 인간 암세포 증식 억제효과)

  • Sim, Jae-Geun;Lee, Jae-Hyeok;Park, Jeong-Suk
    • Journal of Digital Convergence
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    • v.13 no.8
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    • pp.543-548
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    • 2015
  • The present sturdy was designed to determine the effect of the antiproliferation in human cancer cells using the ordinary vegetable soup (VS), the soup with broccoli (VSB) and the soup with tomatoes(VST). Human cancer cells identify the cancer cell growth with MTS, using stomach cancer cell line(AGS), human promyelocytic leukemia (HL-60) and lung cancer cell line (A549). VSB and VST are effective on the cancer cell growth inhibition activities of AGS and have a significance compared with VS. VST has a significance with HL-60 and VSB works well in A549 more than VS. Mixed vegetable soup is considered to applicate with functional materials and using for wellness life.

Inhibitory Effect of Linum usitatissimum and Perilla frutescens as Sources of Omega-3 Fatty Acids on Mutagenicity and Growth of Human Cancer Cell Lines (식물성 오메가-3계 지방산 급원인 아마씨 및 들깨의 항돌연변이 및 암세포 증식 억제 효과)

  • Lim, Sun-Young
    • Journal of Life Science
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    • v.19 no.12
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    • pp.1737-1742
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    • 2009
  • It has been known that Linum usitatissimum and Perilla frutescens are dietary sources of possible chemopreventive compounds such as lignans and $\alpha$-linolenic acid. Here, we investigated and compared the inhibitory effects of methanol extracts from Linum usitatissimum and Perilla frutescens on mutagenicity using the Ames test, and growth of human cancer cells (AGS human gastric adenocarcinoma, HT-29 human colon cancer, Hep 3B hepatocellular carcinoma cells). In the Ames test system using Salmonella typhimurium TA100, aflatoxin $B_1$ ($AFB_1$)-induced mutagenicity was significantly inhibited by treatment with the methanol extract from either Linum usitatissimum or Perilla frutescens (p<0.05) in a dose dependent manner. As for N-methyl-N'-nitro-N-nitrosoguamidine (MNNG)-induced mutagenicity, the methanol extracts (5 mg/assay) from Linum usitatissimum and Perilla frutescens showed 63% and 78% inhibitory rates, respectively, indicating that Perilla frutescens possessed stronger antimutagenic activity than did Linum usitatissimum. Inhibitory effects of methanol extracts from Linum usitatissimum and Perilla frutescens on the growth of human cancer cells (AGS, HT-29 and Hep 3B) appeared to increase dose dependently, and the inhibition was more effective against AGS and HT-29 compared to Hep 3B cells. Our results suggested that the methanol extract from Perilla frutescens showed stronger antimutagenic activity than that from Linum usitatissimumas assayed by the Ames mutagenic test, whereas the methanol extract from Linum usitatissimum was more effective than its counterpart for growth inhibition of human cancer cells. It is concluded that intake of Linum usitatissimum and Perilla frutescens as sources of omega-3 fatty acids will be beneficial for preventing cancer.

Inhibitory Effects of Methanol Extracts from Korean Orysa sartiva and Coix lachryma-jobi var. ma-yuen on Mutagenicity and Growth of Human Cancer Cells (한국산 현미 및 율무 추출물에 의한 돌연변이 및 인체 암세포주 증식 억제 효과)

  • Lim, Sun-Young
    • Journal of Life Science
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    • v.18 no.10
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    • pp.1415-1419
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    • 2008
  • This study was carried out to determine the inhibitory effects of methanol extracts from Korean Orysa sartiva and Coix lachryma-jobi var. ma-yuen on mutagenicity using Ames test and growth of AGS human gastric adenocarcinoma and HT-29 human colon cancer cells. Both treatments of methanol extracts (5 mg/assay) from Orysa sartiva and Coix lachryma-jobi var. ma-yuen to Ames test system inhibited aflatoxin $B_1$ ($AFB_1$) induced mutagenicity by 76%. In case of N-methyl-N'-nitro-N-nitrosoguamidine (MNNG) induced mutagenicity, the methanol extracts (5 mg/assay) from Orysa sartiva and Coix lachryma-jobi var. ma-yuen showed 79% and 69% inhibitory rate, respectively and the inhibitory effect was a little stronger in Orysa sartiva Inhibitory effects of methanol extracts from Orysa sartiva. and Coix lachryma-jobi var. ma-yuen on the growth of AGS and HT-29 human cancer cells were increased as dose dependent patterns and the inhibitory effects on AGS and HT-29 cells were similar. The above results indicate that the consumption of these cereals, which contain many nutrients with good quality, may be recommended as potent functional foods for improving health.

Synergistic Effect of Panax ginseng and Cinnamoum Blume Mixture on the Inhibition of Cancer Cell Growth in vitro (인삼과 계피 혼합물에 의한 in vitro에서 암세포 증식억제의 상승 효과)

  • Chung Hwa Ryung;Lee Ji Young;Kim Dong Chung;Hwang Woo Ik
    • Journal of Ginseng Research
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    • v.23 no.2
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    • pp.99-104
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    • 1999
  • The effects of ginseng and cinnamon extract alone or mixture on the various cancer cell lines in vitro have been examined. Human colon cancer cell line (HT-29), human rectal cancer cell line (HRT-18) and human hepatoma cell line (HepG2) were used for the experiment. When given separately, the proliferation of all cancer cell lines was inhibited in proportion to the concentration of ginseng or cinnamon extract, respectively. Based on the cytotoxic activity, mixture of ginseng and cinnamon extract demonstrated a synergistic inhibition of cancer cell growth. The progression of cell cycle from G1 to S phase was significantly inhibited by ginseng and cinnamon mixture in the HT-29 and HRT-18 cell lines.

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Total Phenolics of Dried Platycodon grandiflorum and Its Effect on Growth of Human Cancer Cell Lines (건조 도라지 추출물 및 분획물의 총 페놀계 화합물 함량 및 인체 암세포 증식 억제효과)

  • Hwang, Seong Yeon;Choi, Hyang Mi;Lim, Sun-Young
    • Korean Journal of Food Science and Technology
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    • v.45 no.1
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    • pp.84-89
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    • 2013
  • We investigated the effects of extracts from dried Platycodon grandiflorum on total phenolic content and growth of cancer cell lines (HT-29 and AGS). Total phenolic contents of acetone/methylene chloride (A+M) extract and methanol (MeOH) fraction were 4.53 and 27.22 TAE mg/100 g, respectively. Among the fractions, n-butanol (n-BuOH) fraction contained the highest phenolic content. Treatments of crude extracts and fractions significantly inhibited the growth of HT-29 and AGS cancer cell lines (p<0.05). Among the fractions, n-BuOH fraction exhibited the highest inhibitory effect and was then sub-fractionated by reverse phase flash column chromatography (rfc). The rfc 1-3 exhibited a higher inhibitory effect on proliferation of both cancer cells. The rfc 1 contained the highest phenol content. Our results showed that n-BuOH fraction possessed a potent inhibitory effect on proliferation of human cancer cells. We suggested that this anticancer activity was partially related to the content of phenolic compounds.

Inhibition effects of extracts and its solvent fractions isolated from Limonium tetragonum on growth of human cancer cells (염생식물 갯질경 [Limonium tetragonum]의 추출물이 암세포성장에 미치는 효과)

  • Kong, Chang-Suk;Um, Young-Ran;Lee, Jung-Im;Kim, You-Ah;Lee, Jeom-Sook;Seo, Young-Wan
    • KSBB Journal
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    • v.23 no.2
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    • pp.177-182
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    • 2008
  • In this study, crude extracts of the halophyte Limonium tetragonum and their solvent fractions were evaluated on anticancer activity in AGS and HT-29 human cancer cells using MTT assay. Each of the crude extracts (MeOH and $CH_2Cl_2$) of Limonium tetragonum showed a significant inhibitory effect on the growth of human cancer cells. The combined crude extracts of MeOH and $CH_2Cl_2$ were partitioned between $CH_2Cl_2$ and water. The organic layer was further partitioned between 85% aq. MeOH and n-hexane, and then the aqueous layer was fractionated with n-BuOH and $H_2O$, successively. Growth inhibition effects of crude extracts and their solvent fractions from Limonium tetragonum increased in a dose-dependent manner. Among them, 85% aq. MeOH, n-hexane and n-BuOH fractions revealed very good inhibition effects on the growth of human cancer cells. These results suggest that we can isolate active compounds from Limonium tetragonum to show much more strong anticancer activity.

Effect of Solvent Extracts from Sargassum hemiphyllum on Inhibition of Growth of Human Cancer Cell Lines and Antioxidant Activity (짝잎모자반(Sargassum hemiphyllum)의 암세포주 증식 억제 및 항산화 효과)

  • Choi, Hyung-Ju;Seo, Young-Wan;Lim, Sun-Young
    • Journal of Life Science
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    • v.17 no.11
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    • pp.1533-1538
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    • 2007
  • This study was carried out to determine the inhibitory effects of solvent extracts from Sargassum hemiphyllum on growth of cancer cell lines (AGS human gastric adenocarcinoma and HT-29 human colon cancer cells) and production of lipid peroxides. Inhibitory effects of acetone with methylene chloride extract from S. hemiphyllum on the growth of AGS and HT-29 cancer cells were increased as dose dependent patterns (p<0.05). The methanol extract was more effective on inhibition of growth of AGS. The treatments of hexane, 85% aq. methanol, butanol and water fractions significantly inhibited the growth of cancer cells (p<0.05) and the inhibitory effect was stronger in HT-29. In DCFH-DA (dichlorodihydrofluorescin diacetate) assay, acetone with methylene chloride and methanol extracts showed a stronger inhibitory effect on the production of cellular lipid peroxides (p<0.05) compared with the butanol and hexane fractions. These results indicate that the consumption of S. hemiphyllum may be recommended as a potent functional food for preventing cellular oxidation and cancer.